FastQCFastQC Report
Fri 10 Feb 2017
SRR2031702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871840
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173560.2955802610425352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163840.2790266764761983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.12123967955530122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107850.061.1391451
GTATCAA189900.040.5766261
GTGGTAT46100.034.7279431
TCAACGC227650.033.5541534
ATCAACG228600.033.497833
TATCAAC235200.032.8422782
TGGTATC47650.032.7009962
CAACGCA233900.032.5548975
AACGCAG241350.031.682636
ACGCAGA269900.028.0325727
CGCAGAG274000.027.3729978
GAGTACT177550.024.56247112-13
GCAGAGT303900.023.9608549
CAGAGTA285950.022.81760810-11
GTACTTT195800.022.0550814-15
GTACATG169750.021.1853751
TACATGG171000.020.1692872
AGAGTAC268100.019.4641210-11
ACATGGG171650.019.2893163
AGTACTT196900.017.78164112-13
Produced by FastQC (version 0.11.5)