FastQCFastQC Report
Fri 10 Feb 2017
SRR2031702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871840
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214020.36448540832175264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213120.36295266901005474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97630.16626815444562523No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61000.10388566445952206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113550.061.63431
GTATCAA201500.039.6840481
GTGGTAT46150.034.488951
TCAACGC243000.032.7843864
ATCAACG243550.032.710913
TATCAAC250550.032.0435032
CAACGCA249800.031.8859635
TGGTATC49250.031.3490832
AACGCAG258050.030.8849586
ACGCAGA294250.026.9562157
CGCAGAG299500.026.4202618
GCAGAGT326800.023.61739
GAGTACT213350.023.3205512-13
CAGAGTA311700.022.26336710-11
GTACTTT232250.021.16713314-15
AGAGTAC292350.019.71578610-11
GTACATG162150.019.690611
TACATGG159400.019.3719222
ACATGGG160350.018.5757453
AGTACTT229850.018.0301112-13
Produced by FastQC (version 0.11.5)