##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031702_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5871840 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.734875779994006 34.0 31.0 34.0 31.0 34.0 2 32.896417817924196 34.0 31.0 34.0 31.0 34.0 3 32.94300014986784 34.0 31.0 34.0 31.0 34.0 4 36.35012619553666 37.0 37.0 37.0 35.0 37.0 5 36.316472860295924 37.0 37.0 37.0 35.0 37.0 6 36.34848326929889 37.0 37.0 37.0 35.0 37.0 7 36.2997511853184 37.0 37.0 37.0 35.0 37.0 8 36.29099720019619 37.0 37.0 37.0 35.0 37.0 9 38.13946786697185 39.0 39.0 39.0 37.0 39.0 10-11 38.10119391877163 39.0 39.0 39.0 36.0 39.0 12-13 38.046692774326274 39.0 39.0 39.0 35.0 39.0 14-15 39.51797613695196 41.0 39.0 41.0 36.5 41.0 16-17 39.49008241028366 41.0 39.0 41.0 36.5 41.0 18-19 39.511010858606504 41.0 39.5 41.0 36.5 41.0 20-21 39.51078512016676 41.0 39.0 41.0 37.0 41.0 22-23 39.42131606106433 41.0 39.0 41.0 36.0 41.0 24-25 39.35145635780266 41.0 39.0 41.0 36.0 41.0 26-27 39.217579072318046 41.0 39.0 41.0 36.0 41.0 28-29 39.11586070124527 40.5 39.0 41.0 35.5 41.0 30-31 38.937307045151094 40.0 38.5 41.0 35.0 41.0 32-33 38.715765586255756 40.0 38.0 41.0 35.0 41.0 34-35 38.565721307120086 40.0 38.0 41.0 35.0 41.0 36-37 38.4556391182321 40.0 38.0 41.0 34.5 41.0 38-39 38.531348265620316 40.0 38.0 41.0 35.0 41.0 40-41 38.43992751846099 40.0 38.0 41.0 35.0 41.0 42-43 38.32328988868906 40.0 38.0 41.0 34.0 41.0 44-45 38.11461620207635 40.0 38.0 41.0 34.0 41.0 46-47 37.86673487356604 40.0 38.0 41.0 33.0 41.0 48-49 37.58259974726832 40.0 38.0 41.0 33.0 41.0 50-51 37.41477884274776 40.0 37.0 41.0 33.0 41.0 52-53 37.45864175454372 40.0 37.0 41.0 33.0 41.0 54-55 37.37477094062508 40.0 37.0 41.0 33.0 41.0 56-57 37.19772251968719 40.0 37.0 41.0 33.0 41.0 58-59 37.011870214447256 40.0 36.0 41.0 32.0 41.0 60-61 36.75620299599444 39.0 36.0 41.0 32.0 41.0 62-63 36.486988575983 39.0 35.0 41.0 31.0 41.0 64-65 36.17621537712199 39.0 35.0 41.0 31.0 41.0 66-67 35.84213815430938 38.0 35.0 40.5 31.0 41.0 68-69 35.47289725196872 37.0 35.0 40.0 30.5 41.0 70-71 35.12346487642715 37.0 35.0 39.5 30.0 41.0 72-73 34.73667146924984 36.0 35.0 39.0 30.0 41.0 74-75 34.354703976947604 36.0 34.5 39.0 29.0 40.5 76-77 33.43619436156298 35.0 33.5 37.0 28.5 39.0 78-79 33.64318901741191 35.0 34.0 37.0 29.0 39.0 80-81 33.42642136025505 35.0 34.0 37.0 29.0 39.0 82-83 33.16234187239434 35.0 34.0 36.0 29.0 38.0 84-85 32.874989015368264 35.0 34.0 36.0 29.0 37.0 86-87 32.630149237717646 35.0 34.0 35.5 29.0 37.0 88-89 32.40701858361263 35.0 34.0 35.0 28.0 36.0 90-91 32.20541968105398 35.0 34.0 35.0 27.0 36.0 92-93 32.03819109512521 35.0 34.0 35.0 27.0 36.0 94-95 31.857159510477125 35.0 33.0 35.0 26.0 36.0 96-97 31.6884713309627 35.0 33.0 35.0 25.5 35.0 98-99 31.476946578925855 35.0 33.0 35.0 25.0 35.0 100-101 30.749132214093027 34.5 32.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1495392940153124 1101 2 0.12299059922362687 1101 3 0.16288969463725067 1101 4 0.10966565549320251 1101 5 0.05245992998064963 1101 6 0.06063684769695499 1101 7 0.12092590341138276 1101 8 0.1085001507695722 1101 9 0.007355767538633984 1101 10-11 0.12235405365385787 1101 12-13 0.12493963270544839 1101 14-15 0.1260102545494135 1101 16-17 0.07164514344372463 1101 18-19 0.13535094436223005 1101 20-21 0.03674030028101072 1101 22-23 -0.08509878872224164 1101 24-25 0.03385827944977393 1101 26-27 0.09086474893955199 1101 28-29 0.047577743382269944 1101 30-31 0.12274228852487568 1101 32-33 0.16181218039109524 1101 34-35 -0.13260544857550371 1101 36-37 0.0612234883496896 1101 38-39 0.089071793449925 1101 40-41 -0.12077640763980924 1101 42-43 0.010528079689620995 1101 44-45 0.10119019331899892 1101 46-47 0.09806811709729146 1101 48-49 0.026860484813035157 1101 50-51 0.16126562587116666 1101 52-53 0.11168320040162882 1101 54-55 0.12344626228316002 1101 56-57 -0.06851439824897199 1101 58-59 0.016802878683769507 1101 60-61 0.028849629058179005 1101 62-63 0.11015519627999737 1101 64-65 0.08458029799526656 1101 66-67 -0.16927092393860477 1101 68-69 -0.12962895053751566 1101 70-71 -0.059622593633577026 1101 72-73 -0.14400153469780008 1101 74-75 -0.12363786763980045 1101 76-77 -0.22358970090366626 1101 78-79 -0.20613468392532752 1101 80-81 -0.15092217173728528 1101 82-83 -0.10466815621243342 1101 84-85 -0.08488780246665328 1101 86-87 -0.11724686650425298 1101 88-89 -0.11272401535725862 1101 90-91 -0.279666000529307 1101 92-93 -0.3883418415111173 1101 94-95 -0.3144193970434159 1101 96-97 -0.17368206845088352 1101 98-99 -0.43026887367032884 1101 100-101 -0.6368231990223165 1102 1 0.1575713542400834 1102 2 0.12767033705652864 1102 3 0.12861776322882434 1102 4 0.08421062026916815 1102 5 0.10197175524477586 1102 6 0.11460707294450856 1102 7 0.17669076758546254 1102 8 0.12588915848279214 1102 9 0.14320056778733914 1102 10-11 0.2001339803823825 1102 12-13 0.1841007973242057 1102 14-15 0.21190401988464913 1102 16-17 0.1642264068114585 1102 18-19 0.18143725242243391 1102 20-21 0.22946788802956064 1102 22-23 0.14579760999514235 1102 24-25 0.07705897889042745 1102 26-27 0.17846197570841582 1102 28-29 0.19230794526620087 1102 30-31 0.08432210740625123 1102 32-33 0.18621051903604524 1102 34-35 0.11963516899135129 1102 36-37 0.22480070190352563 1102 38-39 0.11088021828289385 1102 40-41 0.23543444333000707 1102 42-43 0.29340064454511605 1102 44-45 0.2856704383858286 1102 46-47 0.23596400316555588 1102 48-49 0.25720021557683026 1102 50-51 0.22567985370300647 1102 52-53 0.22971491157482404 1102 54-55 0.36282954848637416 1102 56-57 0.36193827679917234 1102 58-59 0.3312232434622402 1102 60-61 0.43505414735150083 1102 62-63 0.3520193788411419 1102 64-65 0.3436190213537813 1102 66-67 0.3889319595210594 1102 68-69 0.3471479462927931 1102 70-71 0.28420436795884996 1102 72-73 0.32548389476368556 1102 74-75 0.32680600282682803 1102 76-77 0.42410843847197555 1102 78-79 0.3537135740544528 1102 80-81 0.201863977569694 1102 82-83 0.3503442734491884 1102 84-85 0.39420775921198725 1102 86-87 0.3635497303733004 1102 88-89 0.27084183689377284 1102 90-91 0.11942812090378396 1102 92-93 0.26532799548994745 1102 94-95 0.40615274090894715 1102 96-97 0.1787877528591757 1102 98-99 0.12983497488434637 1102 100-101 0.15093015493115658 1103 1 0.16920533290144135 1103 2 0.1181017515822873 1103 3 0.14537981789452203 1103 4 0.09189927946172816 1103 5 0.058661036303846004 1103 6 0.06950375448834478 1103 7 0.13495727857079487 1103 8 0.14635219724346626 1103 9 0.06605418165937493 1103 10-11 0.15323265572472877 1103 12-13 0.06867632170445859 1103 14-15 0.1601624227924674 1103 16-17 0.20530051432761098 1103 18-19 0.20459159125887538 1103 20-21 0.18414574517046134 1103 22-23 0.1686969367163158 1103 24-25 0.06004852895653556 1103 26-27 0.13050380979693443 1103 28-29 0.24343271167588654 1103 30-31 0.18665621485214245 1103 32-33 0.09732565100408408 1103 34-35 0.12887685489371847 1103 36-37 0.12754659584737027 1103 38-39 -0.03864733102823692 1103 40-41 -0.014149749315102156 1103 42-43 0.07802295530427017 1103 44-45 0.1574698655503468 1103 46-47 0.052665022784601945 1103 48-49 0.1748486935263358 1103 50-51 0.008137030600799733 1103 52-53 -0.0377247550766171 1103 54-55 0.14429615019473374 1103 56-57 0.12088140431539074 1103 58-59 0.11778663266206735 1103 60-61 -0.03860988389720177 1103 62-63 -0.03598767366383271 1103 64-65 0.10869979178119848 1103 66-67 0.1123428134144433 1103 68-69 0.05932885908976715 1103 70-71 0.08174143694152747 1103 72-73 0.08015324174139238 1103 74-75 0.1476700147129577 1103 76-77 0.12030259979734836 1103 78-79 0.15780688448061397 1103 80-81 0.12506026214173716 1103 82-83 0.19608398034824148 1103 84-85 0.10902773998213178 1103 86-87 0.12968326264145702 1103 88-89 0.1655155482579218 1103 90-91 0.13728039383955348 1103 92-93 0.1475270958329702 1103 94-95 0.17734158338231154 1103 96-97 0.1032591175263029 1103 98-99 0.1746680175838975 1103 100-101 0.11804676719914653 1104 1 0.1058083356719024 1104 2 0.07946698915168326 1104 3 0.11155843615017602 1104 4 0.08855676789718814 1104 5 0.12030298775367498 1104 6 0.0936367515287273 1104 7 0.15719070316922057 1104 8 0.15963668017088395 1104 9 0.07211399397421303 1104 10-11 0.12552341809918488 1104 12-13 0.16057237334096897 1104 14-15 0.22279138198991433 1104 16-17 0.1309949682647229 1104 18-19 0.20107490511633586 1104 20-21 0.2553562889668086 1104 22-23 0.12357129420626478 1104 24-25 0.16730020595718287 1104 26-27 0.25026432113758545 1104 28-29 0.1975264330785791 1104 30-31 0.20612388606284782 1104 32-33 0.20545546047789998 1104 34-35 0.15157973253334944 1104 36-37 0.19754826204176368 1104 38-39 0.16458527081743313 1104 40-41 0.1933058240682044 1104 42-43 0.2586285449246546 1104 44-45 0.186222395782913 1104 46-47 0.16440956895352343 1104 48-49 0.22720887081532481 1104 50-51 0.24782554091587627 1104 52-53 0.37153971061251667 1104 54-55 0.3265535997646438 1104 56-57 0.24740276725500365 1104 58-59 0.30058297235439824 1104 60-61 0.4909938278371371 1104 62-63 0.4519819040648727 1104 64-65 0.41386266690121687 1104 66-67 0.3314004013043501 1104 68-69 0.2634532813248569 1104 70-71 0.3223920913938656 1104 72-73 0.3066439038486237 1104 74-75 0.31865353490512405 1104 76-77 0.36170161936571077 1104 78-79 0.4200599709117725 1104 80-81 0.4136214127690394 1104 82-83 0.38063041526486785 1104 84-85 0.4313477254112499 1104 86-87 0.40299459679027194 1104 88-89 0.4209779319799125 1104 90-91 0.44463346486487154 1104 92-93 0.48505287843635614 1104 94-95 0.5409063083845034 1104 96-97 0.47269915429341225 1104 98-99 0.4897907267051451 1104 100-101 0.43626052553150174 1105 1 0.13995505311716272 1105 2 0.08517781261173951 1105 3 0.03523618138502371 1105 4 0.04853309802066974 1105 5 0.03335583277008425 1105 6 0.0309268119070083 1105 7 0.07039185261081116 1105 8 0.08186006151387915 1105 9 0.04324509309000035 1105 10-11 0.09980035698519174 1105 12-13 0.14418870898760616 1105 14-15 0.13029983017688096 1105 16-17 0.11236625156235647 1105 18-19 0.12486330961530001 1105 20-21 0.15338873223196714 1105 22-23 0.15805722081668705 1105 24-25 0.15366614416721802 1105 26-27 0.15723619445920178 1105 28-29 0.22781495925021034 1105 30-31 0.15504806965399354 1105 32-33 0.06564735554825774 1105 34-35 0.06511714956472758 1105 36-37 0.21994804335664497 1105 38-39 0.19034939142104434 1105 40-41 0.11469610924237372 1105 42-43 0.0461706545391678 1105 44-45 0.218135844690039 1105 46-47 0.19120891397674455 1105 48-49 0.16323384366966565 1105 50-51 0.2220983475888545 1105 52-53 0.3005391158890163 1105 54-55 0.21146283483003003 1105 56-57 0.1344865794260386 1105 58-59 0.12127828233113291 1105 60-61 0.1548774315205108 1105 62-63 0.12199174118319434 1105 64-65 0.14213719796770619 1105 66-67 0.2854604179165463 1105 68-69 0.24344878281566196 1105 70-71 0.21046970079797234 1105 72-73 0.05899013413398535 1105 74-75 0.14206512710038055 1105 76-77 0.09692838873817777 1105 78-79 0.24676376376154963 1105 80-81 0.19187975291439585 1105 82-83 0.22360651282880184 1105 84-85 0.25394339813675515 1105 86-87 0.14901253542740278 1105 88-89 0.12729952103853748 1105 90-91 0.1642031915298361 1105 92-93 0.1471482844415135 1105 94-95 0.05017178055445015 1105 96-97 0.12379900407346511 1105 98-99 0.1724367311860746 1105 100-101 0.08046927975086504 1106 1 0.1070824621681794 1106 2 0.05979448426472089 1106 3 0.004163104181870381 1106 4 0.0638633056986535 1106 5 0.056458539288939846 1106 6 0.051772003508538944 1106 7 0.08589325996294406 1106 8 0.04146373258650016 1106 9 0.07933640608555237 1106 10-11 0.04358302555758797 1106 12-13 0.04375035037205066 1106 14-15 0.15269515242327003 1106 16-17 0.15520905410438246 1106 18-19 0.14576971603155897 1106 20-21 0.18538050253918215 1106 22-23 0.10197674822487102 1106 24-25 0.14368131837802167 1106 26-27 0.07144410250512578 1106 28-29 0.10199244824205778 1106 30-31 0.11644536843257214 1106 32-33 0.16682619479765037 1106 34-35 0.15207333203398576 1106 36-37 0.1769122291584111 1106 38-39 0.11134743506956823 1106 40-41 0.13199889705585122 1106 42-43 0.0834359634141606 1106 44-45 0.13781842569498082 1106 46-47 0.12425140967111048 1106 48-49 0.1128922431410686 1106 50-51 0.03805995917311833 1106 52-53 0.13307672389994707 1106 54-55 0.029956233972647794 1106 56-57 0.18782753770360472 1106 58-59 0.14675938620914053 1106 60-61 0.2005195673592084 1106 62-63 0.26180814382733786 1106 64-65 0.34440973069535374 1106 66-67 0.25332240474426015 1106 68-69 0.2899052270455158 1106 70-71 0.1768056897443273 1106 72-73 0.22354149260676337 1106 74-75 0.29634415196183284 1106 76-77 0.21015060372898375 1106 78-79 0.1849250624439378 1106 80-81 0.16397494598743378 1106 82-83 0.21046692102062536 1106 84-85 0.2510882651820481 1106 86-87 0.21766373929914096 1106 88-89 0.28869454948745243 1106 90-91 0.2752225476878891 1106 92-93 0.25484258303829677 1106 94-95 0.36065927414918164 1106 96-97 0.3110052927857332 1106 98-99 0.3021487541624843 1106 100-101 0.3943662871704703 1107 1 0.17812537382469174 1107 2 0.12436950536457658 1107 3 0.12324912842885993 1107 4 0.05572553406877745 1107 5 0.05883807687602882 1107 6 0.08048938461799082 1107 7 0.10310376342328453 1107 8 0.11195132934300034 1107 9 0.1210443196807276 1107 10-11 0.1369549067199074 1107 12-13 0.17460895745777094 1107 14-15 0.27013999533603794 1107 16-17 0.2211471517733159 1107 18-19 0.21911832249282526 1107 20-21 0.21924367170249326 1107 22-23 0.23864182781743182 1107 24-25 0.2084410090578146 1107 26-27 0.18775380274853148 1107 28-29 0.2165803609640733 1107 30-31 0.21011600242662354 1107 32-33 0.22943259988516473 1107 34-35 0.2679786057183833 1107 36-37 0.2653668108373495 1107 38-39 0.23244886449191426 1107 40-41 0.2714828134823506 1107 42-43 0.2912067040302446 1107 44-45 0.3189366992160174 1107 46-47 0.36467429424179443 1107 48-49 0.31997129141907266 1107 50-51 0.41799404639348126 1107 52-53 0.37401876241521137 1107 54-55 0.3647744030545539 1107 56-57 0.3630324125081472 1107 58-59 0.3181607583214756 1107 60-61 0.42771160386155316 1107 62-63 0.3903182033184649 1107 64-65 0.38328214944100125 1107 66-67 0.371978702288267 1107 68-69 0.3474850804012988 1107 70-71 0.35783194159598963 1107 72-73 0.342691375562282 1107 74-75 0.35614322516066466 1107 76-77 0.3748872129478613 1107 78-79 0.4136231732174167 1107 80-81 0.4239219731400041 1107 82-83 0.40889559241773554 1107 84-85 0.46323521500276854 1107 86-87 0.5046850875033755 1107 88-89 0.5211918150500381 1107 90-91 0.44766918791285804 1107 92-93 0.46544339704448845 1107 94-95 0.5026287239569278 1107 96-97 0.5611936750812951 1107 98-99 0.6161375508865632 1107 100-101 0.7302429470003773 1108 1 0.04155275402477798 1108 2 0.008670570215208784 1108 3 -1.8468856327302774E-4 1108 4 0.018529604271712685 1108 5 0.054278461072179596 1108 6 -0.015332556830095712 1108 7 -0.03892377701183847 1108 8 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-0.565358599211585 2316 50-51 -0.4710759682937109 2316 52-53 -0.5540538760972495 2316 54-55 -0.6037903514249621 2316 56-57 -0.5452123399359721 2316 58-59 -0.561792439741474 2316 60-61 -0.6028906926752526 2316 62-63 -0.5035395800667359 2316 64-65 -0.5189961788698483 2316 66-67 -0.45745231156212185 2316 68-69 -0.578158942839849 2316 70-71 -0.49555578601554373 2316 72-73 -0.6164429491319083 2316 74-75 -0.5080438505292477 2316 76-77 -0.8771081681423567 2316 78-79 -0.5525693153977969 2316 80-81 -0.3833723055236007 2316 82-83 -0.35007737231045866 2316 84-85 -0.36033718949243365 2316 86-87 -0.30841080747576655 2316 88-89 -0.32461535871788527 2316 90-91 -0.39131932899926625 2316 92-93 -0.2813077409800613 2316 94-95 -0.2626106229573537 2316 96-97 -0.24382201781840607 2316 98-99 -0.24697744736187133 2316 100-101 6.562169588590905E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 17.0 5 53.0 6 113.0 7 234.0 8 781.0 9 1687.0 10 2972.0 11 4911.0 12 7505.0 13 10796.0 14 14390.0 15 18431.0 16 24284.0 17 36504.0 18 37752.0 19 22531.0 20 15815.0 21 15233.0 22 15755.0 23 16686.0 24 18631.0 25 22045.0 26 25255.0 27 32020.0 28 40990.0 29 52863.0 30 67520.0 31 87576.0 32 113722.0 33 151933.0 34 218671.0 35 346669.0 36 616842.0 37 1302552.0 38 1926686.0 39 600783.0 40 622.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.532962276704275 19.513330546661255 14.341209258973475 23.612497917660992 2 16.54706190904384 21.496362298700237 38.48899152565465 23.467584266601268 3 18.678845865634834 25.509591672684166 30.91829366322874 24.893268798452254 4 13.967474481648503 18.71706887080496 36.557916118400044 30.757540529146493 5 14.16457192294068 36.495170168124474 34.60675018392872 14.733507725006131 6 30.20061854546445 36.273944794136085 18.36509509795907 15.160341562440394 7 25.840383934167143 32.97850418267528 22.890865554919753 18.290246328237828 8 24.636144717839723 33.99685618136734 21.460496198806506 19.906502901986432 9 25.58719243031145 16.370830949072182 21.310764598490422 36.731212022125945 10-11 23.9100472305345 26.2233096729354 28.937460287129905 20.929182809400192 12-13 25.073899335455213 24.674986831212504 28.382998920781038 21.86811491255125 14-15 22.59290049692315 26.342189368959456 26.569733286885604 24.49517684723179 16-17 21.618408579911787 28.76223525408557 27.617451116036378 22.00190504996626 18-19 21.418106825370025 27.257941745813554 29.947296718377075 21.37665471043935 20-21 22.35719978746015 26.37210823183193 29.7884393988937 21.48225258181422 22-23 22.2539527643805 26.414829082536308 29.456362911795964 21.87485524128723 24-25 21.89909806806725 26.774264966347854 29.61195298237009 21.7146839832148 26-27 21.67836520735071 26.939924026897987 29.865075790024036 21.516634975727268 28-29 21.585287104492963 26.779078602943233 30.037318214955743 21.598316077608057 30-31 22.001363798307718 26.56555802989048 29.743601490232358 21.689476681569445 32-33 21.88848306266125 26.781195893719417 29.434736282161904 21.895584761457428 34-35 21.955899873705224 26.9136809690037 29.510618384533956 21.619800772757117 36-37 21.891944918572424 26.88709274715333 29.63814109889676 21.582821235377487 38-39 21.733638859369464 26.99509864028993 29.670145984904224 21.601116515436388 40-41 21.950308591514755 26.817982097604837 29.609364355977004 21.6223449549034 42-43 22.005299871931115 26.934453254857083 29.23315349192076 21.827093381291043 44-45 21.986779271914767 27.1007469549579 29.038972451565435 21.873501321561896 46-47 22.006611215564455 27.14655882994087 28.900157361236 21.946672593258672 48-49 21.946689623695466 27.30102489168642 28.8854686094989 21.866816875119213 50-51 21.978766451401945 27.477894493037958 28.636185590888037 21.90715346467206 52-53 22.223340554238536 27.479691204120005 28.353803918362896 21.943164323278562 54-55 22.092402040927546 27.548272773100084 28.339694201476878 22.01963098449549 56-57 22.029868152901656 27.762543577698267 28.22731275267316 21.980275516726913 58-59 21.968468903819897 27.730419303376426 28.46549772238517 21.83561407041851 60-61 22.055506544908322 27.64216399227486 28.523342314203287 21.77898714861353 62-63 21.953287435177618 27.65759731713372 28.421665780297744 21.96744946739092 64-65 21.990962417547344 27.611895560793865 28.388407928670777 22.008734092988014 66-67 21.901416240694243 27.763507023808216 28.263373711832052 22.071703023665496 68-69 21.8630769931659 27.715087264541573 28.40502873336873 22.0168070089238 70-71 21.897780633217995 27.56665827916583 28.437655535984717 22.097905551631456 72-73 21.99918299060354 27.621985744999904 28.23320994169462 22.145621322701935 74-75 21.94498826955748 27.753128472828376 28.118964996018885 22.182918261595255 76-77 21.983422121600913 27.794101254985637 28.00389424566702 22.21858237774643 78-79 21.908225750813994 27.86802298882091 27.989561311626115 22.23418994873898 80-81 21.943398357004497 27.86783752762781 27.96145315156704 22.227310963800655 82-83 22.003984536035066 27.785513280253877 27.999200503883536 22.211301679827525 84-85 22.037432199259857 27.789121720003347 27.85445192750084 22.318994153235952 86-87 21.960443563989514 27.834267075769382 27.855453400984242 22.349835959256858 88-89 21.954459880055754 27.786714542862967 27.881693749875996 22.377131827205286 90-91 21.976331098940026 27.740435706695006 27.92525852203057 22.357974672334397 92-93 22.031816262023487 27.691379533502275 27.947840881222923 22.328963323251312 94-95 21.994451483691655 27.660579988555543 27.881490299463202 22.4634782282896 96-97 21.954827265770337 27.622030135876525 27.853456800625565 22.569685797727576 98-99 21.95255079458098 27.653058148582804 27.76329622228955 22.631094834546666 100-101 21.92070832396294 27.804796384876024 27.60971062164917 22.66478466951186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3880.0 1 2422.0 2 1057.5 3 1096.0 4 860.0 5 852.5 6 1477.0 7 3124.0 8 5330.5 9 6495.5 10 5750.0 11 4669.0 12 4571.0 13 4944.5 14 5439.5 15 5901.5 16 6274.5 17 6715.5 18 7297.0 19 7978.0 20 8869.0 21 10081.0 22 11840.0 23 14281.5 24 17378.5 25 21678.5 26 27934.0 27 36433.0 28 47159.5 29 60728.5 30 76478.0 31 93025.0 32 109086.0 33 123354.5 34 136788.0 35 149319.5 36 163258.0 37 174631.0 38 180360.5 39 184556.0 40 190356.5 41 197465.5 42 203485.0 43 210436.0 44 216836.5 45 222173.5 46 226497.5 47 227651.5 48 228155.5 49 225972.0 50 217000.5 51 206527.5 52 197446.5 53 188204.0 54 176844.5 55 162188.0 56 145923.5 57 128910.5 58 114891.0 59 100587.5 60 81992.5 61 66309.5 62 53160.5 63 39359.5 64 28292.5 65 20912.0 66 15794.5 67 12147.5 68 9520.5 69 7190.0 70 5267.5 71 3903.5 72 2680.5 73 1935.5 74 1510.5 75 1126.5 76 728.5 77 404.0 78 243.5 79 136.0 80 73.5 81 45.0 82 40.0 83 25.0 84 15.0 85 13.0 86 9.0 87 7.0 88 4.5 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017677593394915394 2 0.0 3 0.0013624349437314368 4 0.018137415188424752 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.109131038992888E-5 12-13 1.703043679664296E-5 14-15 1.1069783917817923E-4 16-17 0.001328374070138151 18-19 7.663696558489332E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.2576091991607405E-4 28-29 0.005287950625357639 30-31 6.812174718657184E-5 32-33 9.366740238153628E-5 34-35 0.01115493610180114 36-37 0.0041213657047875964 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 8.51521839832148E-6 58-59 2.980326439412518E-4 60-61 0.0017030436796642962 62-63 0.008642946674296303 64-65 0.0023331698411400855 66-67 0.004674854900678493 68-69 0.0031080547153873404 70-71 1.277282759748222E-4 72-73 0.007263481293768223 74-75 0.006216109430774681 76-77 0.0016945284612659745 78-79 5.023978855009674E-4 80-81 0.008361944467151694 82-83 0.010933540423444781 84-85 0.0022224720019619064 86-87 0.002179895909970299 88-89 4.8536744870432435E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 2.554565519496444E-5 98-99 5.109131038992888E-5 100-101 0.001081432736586828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5871840.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.851564014706724 #Duplication Level Percentage of deduplicated Percentage of total 1 54.33457081683546 21.653176271187345 2 20.292273695463503 16.173576883574263 3 9.666062149676437 11.556230835838926 4 5.210935518881306 8.306557216288295 5 2.9668007304190067 5.911582461358587 6 1.953346465941081 4.670634703821128 7 1.299424974821467 3.624888229648445 8 0.8735708738341504 2.785053247998795 9 0.6309714206349364 2.263067816479525 >10 2.6521317616970914 17.288285490021245 >50 0.09226764802831652 2.459949027452929 >100 0.026147463185850837 1.7104061468175102 >500 7.696257177084203E-4 0.21922691728427737 >1k 5.558301899413841E-4 0.37927114192215655 >5k 8.551233685885764E-5 0.2702896860592656 >10k+ 8.551233685885764E-5 0.7278039242473346 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21402 0.36448540832175264 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21312 0.36295266901005474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9763 0.16626815444562523 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6100 0.10388566445952206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.703043679664296E-5 0.0 0.0 0.0 0.0 6 1.703043679664296E-5 0.0 0.0 0.0 0.0 7 5.1091310389928884E-5 0.0 0.0 0.0 0.0 8 5.1091310389928884E-5 0.0 0.0 0.0 0.0 9 5.1091310389928884E-5 0.0 0.0 0.0 1.703043679664296E-5 10-11 1.0218262077985777E-4 0.0 0.0 0.0 1.703043679664296E-5 12-13 1.1921305757650072E-4 8.51521839832148E-6 0.0 0.0 1.703043679664296E-5 14-15 1.6178914956810813E-4 1.703043679664296E-5 0.0 0.0 5.1091310389928884E-5 16-17 1.9585002316139404E-4 1.703043679664296E-5 0.0 0.0 5.1091310389928884E-5 18-19 2.213956783563585E-4 1.703043679664296E-5 0.0 3.406087359328592E-5 1.0218262077985777E-4 20-21 2.895174255429303E-4 1.703043679664296E-5 0.0 5.1091310389928884E-5 1.0218262077985777E-4 22-23 3.065478623395733E-4 2.5545655194964442E-5 0.0 1.362434943731437E-4 1.362434943731437E-4 24-25 4.513065751110385E-4 3.406087359328592E-5 0.0 2.895174255429303E-4 1.5327393116978664E-4 26-27 4.938826671026459E-4 3.406087359328592E-5 0.0 5.534891958908962E-4 1.8733480476307258E-4 28-29 4.938826671026459E-4 3.406087359328592E-5 0.0 0.0010558870813918637 2.0436524155971553E-4 30-31 5.620044142892177E-4 3.406087359328592E-5 0.0 0.00212880459958037 2.0436524155971553E-4 32-33 5.790348510858606E-4 3.406087359328592E-5 0.0 0.004019183084007739 2.0436524155971553E-4 34-35 6.301261614757895E-4 3.406087359328592E-5 0.0 0.006531172511512575 2.0436524155971553E-4 36-37 6.812174718657184E-4 3.406087359328592E-5 0.0 0.009077222812610697 2.1288045995803703E-4 38-39 6.812174718657184E-4 3.406087359328592E-5 0.0 0.010890964331453173 2.2991089675467995E-4 40-41 6.812174718657184E-4 3.406087359328592E-5 0.0 0.01220230796479468 2.3842611515300144E-4 42-43 6.897326902640399E-4 3.406087359328592E-5 0.0 0.01525075615139377 2.554565519496444E-4 44-45 7.237935638573258E-4 3.406087359328592E-5 0.0 0.018452478269162645 2.895174255429303E-4 46-47 7.748848742472547E-4 3.406087359328592E-5 0.0 0.02191817215727949 3.065478623395733E-4 48-49 8.004305294422191E-4 3.406087359328592E-5 0.0 0.029760688302133576 3.2357829913621627E-4 50-51 8.17460966238862E-4 3.406087359328592E-5 0.0 0.03609601079048476 3.2357829913621627E-4 52-53 8.940979318237555E-4 3.406087359328592E-5 0.0 0.047140249053107715 3.406087359328592E-4 54-55 9.537044606120058E-4 3.406087359328592E-5 0.0 0.06795995803700373 3.831848279244666E-4 56-57 9.537044606120058E-4 3.406087359328592E-5 0.0 0.08843054306656857 4.1724570151775253E-4 58-59 9.537044606120058E-4 3.406087359328592E-5 0.0 0.106321016921442 4.25760919916074E-4 60-61 9.877653342052917E-4 3.406087359328592E-5 0.0 0.1294313196544865 4.25760919916074E-4 62-63 9.877653342052917E-4 3.406087359328592E-5 0.0 0.1603160167851985 4.42791356712717E-4 64-65 9.877653342052917E-4 3.406087359328592E-5 0.0 0.1952028665631216 4.42791356712717E-4 66-67 9.877653342052917E-4 3.406087359328592E-5 0.0 0.24850813373661407 4.42791356712717E-4 68-69 0.0010218262077985776 3.406087359328592E-5 0.0 0.31428819586364753 4.42791356712717E-4 70-71 0.0010218262077985776 3.406087359328592E-5 0.0 0.37571698138913867 4.768522303060029E-4 72-73 0.0010218262077985776 3.406087359328592E-5 0.0 0.44262956756314886 4.768522303060029E-4 74-75 0.0010218262077985776 3.406087359328592E-5 0.0 0.5211569116324695 4.768522303060029E-4 76-77 0.0010558870813918634 3.406087359328592E-5 0.0 0.6084804763072563 4.768522303060029E-4 78-79 0.0010729175181885065 3.406087359328592E-5 0.0 0.7017817242976647 4.768522303060029E-4 80-81 0.0010729175181885065 3.406087359328592E-5 0.0 0.799102155372081 4.8536744870432435E-4 82-83 0.0010729175181885065 3.406087359328592E-5 0.0 0.9060873593285921 4.938826671026459E-4 84-85 0.0010729175181885065 3.406087359328592E-5 0.0 1.0368981443636067 4.938826671026459E-4 86-87 0.0010729175181885065 3.406087359328592E-5 0.0 1.2029108422572823 5.109131038992888E-4 88-89 0.0010729175181885065 3.406087359328592E-5 0.0 1.3866692552930597 5.279435406959317E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11355 0.0 61.6343 1 GTATCAA 20150 0.0 39.684048 1 GTGGTAT 4615 0.0 34.48895 1 TCAACGC 24300 0.0 32.784386 4 ATCAACG 24355 0.0 32.71091 3 TATCAAC 25055 0.0 32.043503 2 CAACGCA 24980 0.0 31.885963 5 TGGTATC 4925 0.0 31.349083 2 AACGCAG 25805 0.0 30.884958 6 ACGCAGA 29425 0.0 26.956215 7 CGCAGAG 29950 0.0 26.420261 8 GCAGAGT 32680 0.0 23.6173 9 GAGTACT 21335 0.0 23.32055 12-13 CAGAGTA 31170 0.0 22.263367 10-11 GTACTTT 23225 0.0 21.167133 14-15 AGAGTAC 29235 0.0 19.715786 10-11 GTACATG 16215 0.0 19.69061 1 TACATGG 15940 0.0 19.371922 2 ACATGGG 16035 0.0 18.575745 3 AGTACTT 22985 0.0 18.03011 12-13 >>END_MODULE