FastQCFastQC Report
Fri 10 Feb 2017
SRR2031701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468351
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78540.3181881345076126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77660.3146230013478634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.14463097023073299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.057.6762161
GTATCAA75850.038.27161
TCAACGC90150.031.6197834
ATCAACG90700.031.4280433
TATCAAC93250.030.9774572
CAACGCA92400.030.9501425
GTGGTAT18400.030.7271
TGGTATC18000.030.6179752
AACGCAG94550.030.3400736
ACGCAGA107250.026.5707597
CGCAGAG109600.025.7875048
GAGTACT77000.023.78521312-13
GCAGAGT120900.022.9843629
CAGAGTA115150.022.0710810-11
GTACTTT85600.021.03524814-15
AGAGTAC105350.019.70518910-11
GTACATG56500.019.340621
TACATGG58650.018.0645792
AGTACTT86300.017.20333312-13
ACTTTTT106950.017.03596516-17
Produced by FastQC (version 0.11.5)