##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031701_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2468351
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03587536780628	33.0	31.0	34.0	30.0	34.0
2	32.34091180711333	34.0	31.0	34.0	31.0	34.0
3	32.444763325799286	34.0	31.0	34.0	31.0	34.0
4	35.885750041221854	37.0	35.0	37.0	35.0	37.0
5	35.88996419066818	37.0	37.0	37.0	35.0	37.0
6	35.92018031471213	37.0	37.0	37.0	35.0	37.0
7	35.90474814967563	37.0	37.0	37.0	35.0	37.0
8	35.89817007386713	37.0	37.0	37.0	35.0	37.0
9	37.67791128571261	39.0	38.0	39.0	35.0	39.0
10-11	37.6100270990633	39.0	38.0	39.0	35.0	39.0
12-13	37.557441384957	39.0	38.0	39.0	35.0	39.0
14-15	38.94301701824416	41.0	39.0	41.0	36.0	41.0
16-17	38.86963381626033	41.0	39.0	41.0	36.0	41.0
18-19	38.89262487385303	41.0	39.0	41.0	36.0	41.0
20-21	38.90200319970701	41.0	39.0	41.0	35.5	41.0
22-23	38.80185395026882	41.0	39.0	41.0	35.0	41.0
24-25	38.73255586421867	41.0	39.0	41.0	35.0	41.0
26-27	38.6178302842667	40.0	38.5	41.0	35.0	41.0
28-29	38.504380252241276	40.0	38.0	41.0	34.0	41.0
30-31	38.331501476086665	40.0	38.0	41.0	34.0	41.0
32-33	38.106491945432396	40.0	38.0	41.0	34.0	41.0
34-35	37.9281188129241	40.0	38.0	41.0	33.5	41.0
36-37	37.755393783137	40.0	38.0	41.0	33.0	41.0
38-39	37.56367935516464	40.0	37.5	41.0	33.0	41.0
40-41	37.45437946224017	40.0	37.5	41.0	33.0	41.0
42-43	37.41761625473849	40.0	37.0	41.0	33.0	41.0
44-45	37.264186900485385	40.0	37.0	41.0	32.0	41.0
46-47	37.151757185262554	40.0	37.5	41.0	31.5	41.0
48-49	37.15854451007981	40.0	38.0	41.0	31.5	41.0
50-51	36.594102500009114	39.5	36.5	40.5	30.5	41.0
52-53	36.61129434185008	39.5	37.0	40.5	31.0	41.0
54-55	36.838215472596886	40.0	37.0	41.0	31.0	41.0
56-57	36.709960414868064	40.0	36.5	41.0	31.0	41.0
58-59	36.522418813207686	40.0	36.0	41.0	31.0	41.0
60-61	36.28447980048218	39.0	35.5	41.0	30.0	41.0
62-63	36.00368768461212	39.0	35.0	41.0	29.5	41.0
64-65	35.728894310412095	39.0	35.0	41.0	29.0	41.0
66-67	35.43427920097263	38.0	35.0	41.0	29.0	41.0
68-69	35.098133733816624	37.5	35.0	40.0	29.0	41.0
70-71	34.735619447963444	37.0	35.0	39.5	28.0	41.0
72-73	34.36541723604139	36.5	35.0	39.0	28.0	41.0
74-75	33.9612676641207	36.0	34.0	39.0	27.5	41.0
76-77	33.58263087381009	35.5	34.0	38.0	26.5	40.0
78-79	33.19545842548325	35.0	34.0	37.0	26.0	39.0
80-81	32.848919784909036	35.0	34.0	37.0	26.0	39.0
82-83	32.523447435150025	35.0	34.0	36.0	26.0	38.5
84-85	32.205240057025925	35.0	34.0	36.0	25.0	37.0
86-87	31.93143175342567	35.0	33.5	36.0	25.0	37.0
88-89	31.666115151370285	35.0	33.0	35.0	24.0	36.5
90-91	31.43036322629966	35.0	33.0	35.0	23.5	36.0
92-93	31.222546145179514	35.0	33.0	35.0	22.0	36.0
94-95	30.97888671424769	35.0	33.0	35.0	19.5	36.0
96-97	30.722865589213203	35.0	32.5	35.0	17.5	35.5
98-99	30.478977868220525	35.0	32.0	35.0	4.5	35.0
100-101	29.89177511626183	34.5	31.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05276338118621027
1101	2	0.09261198456368902
1101	3	0.2027894127083698
1101	4	0.22727827539609535
1101	5	0.05071649129600786
1101	6	0.20256833150182274
1101	7	0.14360956291118043
1101	8	0.1492457011785504
1101	9	0.25165438552028974
1101	10-11	0.17692650146147315
1101	12-13	0.1955821578996293
1101	14-15	0.15638661806242027
1101	16-17	0.2636113028541942
1101	18-19	0.32812464585328627
1101	20-21	0.29640086551693656
1101	22-23	0.2869613986918438
1101	24-25	0.08783611620118137
1101	26-27	-0.08566653956189185
1101	28-29	-0.06010863198429206
1101	30-31	-0.024936071636169288
1101	32-33	-0.02395218398486776
1101	34-35	0.15740046177047873
1101	36-37	-0.01946122694077701
1101	38-39	0.21080255598084108
1101	40-41	-0.05900179045457321
1101	42-43	-0.12631208827544782
1101	44-45	-0.22183566137703536
1101	46-47	-0.24005166840810688
1101	48-49	-0.10597461119760254
1101	50-51	0.01609905954202162
1101	52-53	-0.06411013511612396
1101	54-55	0.12457533882160732
1101	56-57	-0.05934197558435983
1101	58-59	-0.2862060671639739
1101	60-61	-0.4563245429324212
1101	62-63	-0.17309823150775827
1101	64-65	0.0625986149120834
1101	66-67	-0.2553332795641552
1101	68-69	-0.391899652671853
1101	70-71	-0.2947284892168298
1101	72-73	0.05557423218607482
1101	74-75	0.06501836167223729
1101	76-77	-0.15566078332797417
1101	78-79	-0.2555020688903227
1101	80-81	-0.320808667001657
1101	82-83	-0.3632851825269725
1101	84-85	-0.40747420146797
1101	86-87	-0.4877504497067342
1101	88-89	-0.5337830972718365
1101	90-91	-0.7789281115837419
1101	92-93	-0.7417032145474707
1101	94-95	-0.5160975232032534
1101	96-97	-0.6307260556952023
1101	98-99	-0.6197279663201165
1101	100-101	-0.5586313694463385
1102	1	0.014954167614654068
1102	2	0.11764136218765486
1102	3	0.15116869864192495
1102	4	0.24949921481778148
1102	5	0.24511447151164134
1102	6	0.22239543087202662
1102	7	0.2047752079614611
1102	8	0.04662695337684397
1102	9	0.17534751755537314
1102	10-11	0.20646902495685282
1102	12-13	0.25289962507858377
1102	14-15	0.41402502403657593
1102	16-17	0.3175286400824646
1102	18-19	0.30069337338377267
1102	20-21	0.24804301428540043
1102	22-23	0.34394134762445105
1102	24-25	0.16813793828509915
1102	26-27	0.18460894334538835
1102	28-29	0.14434463991781143
1102	30-31	0.10870571556154829
1102	32-33	0.09950330925044426
1102	34-35	0.24404711227385434
1102	36-37	0.17961021192289195
1102	38-39	0.04746990283012309
1102	40-41	0.10112158661628712
1102	42-43	0.20968715815381955
1102	44-45	0.03195557357504697
1102	46-47	0.05525654828733195
1102	48-49	0.23298320584580523
1102	50-51	0.06467450208583614
1102	52-53	0.07840076346775504
1102	54-55	0.04511322902044412
1102	56-57	0.09108723306792399
1102	58-59	0.009841065016090056
1102	60-61	-0.1538889521982938
1102	62-63	-0.02697990389780358
1102	64-65	-0.05559497221886289
1102	66-67	-0.08655636872088479
1102	68-69	-0.12183404189919145
1102	70-71	-0.035794044500910616
1102	72-73	-0.004164148102233867
1102	74-75	-0.2779982884821024
1102	76-77	0.07460072003668472
1102	78-79	-0.20931308283674355
1102	80-81	-0.001988394516914127
1102	82-83	0.1611701791467084
1102	84-85	0.1468556083404451
1102	86-87	0.04279836368785084
1102	88-89	0.01356734533909787
1102	90-91	-0.021179175935284178
1102	92-93	0.10258284877932056
1102	94-95	-0.21863822900010987
1102	96-97	-0.4741247492076006
1102	98-99	-0.3839991596020589
1102	100-101	-0.42968261852115575
1103	1	0.03125070512530925
1103	2	0.18168306116831445
1103	3	0.20537673079452645
1103	4	0.231249269448071
1103	5	0.36820016180287496
1103	6	0.23873931463590736
1103	7	0.2221778482113521
1103	8	0.2864635766677708
1103	9	0.3115858256016679
1103	10-11	0.18280768135159775
1103	12-13	0.3290362565104914
1103	14-15	0.5329056814853601
1103	16-17	0.3435863253862692
1103	18-19	0.38281427129152945
1103	20-21	0.1633956260418401
1103	22-23	0.2613657328729886
1103	24-25	0.17136391059718648
1103	26-27	0.23351698021119205
1103	28-29	0.09436820592620165
1103	30-31	0.1096815625453118
1103	32-33	0.03888842924427394
1103	34-35	0.1450241375580461
1103	36-37	0.1755621072726754
1103	38-39	0.07501492461796744
1103	40-41	0.20400103148682547
1103	42-43	0.2291297577844844
1103	44-45	0.11857447265683163
1103	46-47	0.19768157624518778
1103	48-49	0.28710232987584305
1103	50-51	0.2047606037110299
1103	52-53	0.13682379045725668
1103	54-55	0.06875314898170615
1103	56-57	0.0224963304041097
1103	58-59	0.05294382995158031
1103	60-61	0.0599820003397582
1103	62-63	0.19885626092715114
1103	64-65	0.34634922347327546
1103	66-67	0.21393819610064213
1103	68-69	0.13427833796772148
1103	70-71	0.2152309697458108
1103	72-73	0.08082130524349651
1103	74-75	0.16364081309596656
1103	76-77	0.11795189212425328
1103	78-79	0.05764898017447706
1103	80-81	0.09280962336754328
1103	82-83	0.061021391939192426
1103	84-85	-0.1195617585223161
1103	86-87	-0.011432698882043013
1103	88-89	2.1710835031996112E-5
1103	90-91	0.025557360375653815
1103	92-93	-0.5189016383600631
1103	94-95	-0.1664097415522221
1103	96-97	-0.0839457760857023
1103	98-99	-0.20679338121680146
1103	100-101	-0.10503643000165752
1104	1	0.05126824093967741
1104	2	0.13904535999557055
1104	3	0.22154218441209395
1104	4	0.12059165570595809
1104	5	0.07942090913497424
1104	6	0.22935982781208963
1104	7	0.1617329105649361
1104	8	0.10985080450809903
1104	9	0.1331987685647178
1104	10-11	0.23991139420315477
1104	12-13	0.1895341949653755
1104	14-15	0.2626602525462971
1104	16-17	0.3292382198028676
1104	18-19	0.21871124268869124
1104	20-21	0.26320215238748546
1104	22-23	0.3348415196813761
1104	24-25	0.3514796014244226
1104	26-27	0.2767421415168769
1104	28-29	0.2708985783914528
1104	30-31	0.2420267551545976
1104	32-33	0.33971242618844855
1104	34-35	0.307379174653434
1104	36-37	0.32482107936753835
1104	38-39	0.22748996920800124
1104	40-41	0.33806316044234563
1104	42-43	0.28690674653112325
1104	44-45	0.3238647193693822
1104	46-47	0.322215257120277
1104	48-49	0.470126129516224
1104	50-51	0.38794661481014714
1104	52-53	0.35864076582947746
1104	54-55	0.3580537142891629
1104	56-57	0.3127393163371508
1104	58-59	0.2721953384560365
1104	60-61	0.3161940159956984
1104	62-63	0.2834014529153279
1104	64-65	0.21225645342536126
1104	66-67	0.2578733819088441
1104	68-69	0.2454276584290369
1104	70-71	0.2932406030568231
1104	72-73	0.20528418035102192
1104	74-75	0.168918844778446
1104	76-77	0.2851762749805573
1104	78-79	0.20452550381832424
1104	80-81	0.19245994225228458
1104	82-83	0.1545882117768116
1104	84-85	0.2728320043591026
1104	86-87	0.22982118430707388
1104	88-89	0.354123528286582
1104	90-91	0.4042825046009959
1104	92-93	0.288546113919125
1104	94-95	0.3552250435408837
1104	96-97	0.33544299623131124
1104	98-99	0.17266642460562664
1104	100-101	0.08376052410812918
1105	1	-0.1437939165992681
1105	2	0.041171248450460496
1105	3	0.01256661749325616
1105	4	-0.03286748680358187
1105	5	0.10423361323947944
1105	6	0.22346423784145486
1105	7	0.19793887659097464
1105	8	0.16893476978403754
1105	9	0.20569760225328082
1105	10-11	0.22060550959023573
1105	12-13	0.10895350908277379
1105	14-15	0.13188015027679256
1105	16-17	0.20452751077699105
1105	18-19	0.10435554971675032
1105	20-21	0.15435099202195346
1105	22-23	-0.029102533959807886
1105	24-25	0.06555263538923839
1105	26-27	0.07351351703257336
1105	28-29	0.003705775676458245
1105	30-31	0.14940104507675045
1105	32-33	0.1412976949889284
1105	34-35	0.08964483752996699
1105	36-37	0.1820805676541113
1105	38-39	0.24451354242034284
1105	40-41	0.280429080364776
1105	42-43	0.11149808015107965
1105	44-45	0.13850352872548655
1105	46-47	0.2578257182478083
1105	48-49	0.3063073971824153
1105	50-51	0.09410702608357724
1105	52-53	0.24524556462932168
1105	54-55	0.2930764459277597
1105	56-57	0.3467841758766639
1105	58-59	0.2086290782450959
1105	60-61	0.3436709413243264
1105	62-63	0.44320441488900286
1105	64-65	0.3718673170446678
1105	66-67	0.3092551179291121
1105	68-69	0.3783071212854523
1105	70-71	0.35878730582578555
1105	72-73	0.1803159680549058
1105	74-75	0.2299169329424373
1105	76-77	0.5092018332092323
1105	78-79	0.48909177317680985
1105	80-81	0.4154815578909208
1105	82-83	0.4567154723628022
1105	84-85	0.47699596487831997
1105	86-87	0.4447403657851332
1105	88-89	0.41911909172935324
1105	90-91	0.20927980607649488
1105	92-93	0.24707345134445902
1105	94-95	0.3160072497447466
1105	96-97	0.21703036896615302
1105	98-99	0.21061042336156177
1105	100-101	0.21424333130541484
1106	1	0.09005749626616932
1106	2	0.14626953599710646
1106	3	0.193525799241975
1106	4	0.13712714313365382
1106	5	0.21033970181279216
1106	6	0.18042347348327326
1106	7	0.2052490910662712
1106	8	0.23754992875906566
1106	9	0.25726772006745335
1106	10-11	0.20888914899148148
1106	12-13	0.21151785841016846
1106	14-15	0.22639508347820936
1106	16-17	0.3632899519035817
1106	18-19	0.22062443395629572
1106	20-21	0.18484813664986888
1106	22-23	0.2483204903286662
1106	24-25	0.366409824440467
1106	26-27	0.24206852745511043
1106	28-29	0.24212010585255683
1106	30-31	0.2867386680444497
1106	32-33	0.3123280348811832
1106	34-35	0.2927660180968914
1106	36-37	0.1617814416847665
1106	38-39	0.26629905941880594
1106	40-41	0.30429660341850706
1106	42-43	0.2969800480962306
1106	44-45	0.2982586615623575
1106	46-47	0.3241973843338428
1106	48-49	0.40198863487602665
1106	50-51	0.29575066948830653
1106	52-53	0.4344331645962214
1106	54-55	0.3677877809946537
1106	56-57	0.3842954116262689
1106	58-59	0.34637061077247466
1106	60-61	0.2926071299655888
1106	62-63	0.4371974511301957
1106	64-65	0.5367486463565498
1106	66-67	0.4259063544871111
1106	68-69	0.3862889785572605
1106	70-71	0.41100779790830444
1106	72-73	0.34809097204876593
1106	74-75	0.30610147447573866
1106	76-77	0.42200673825151114
1106	78-79	0.3824660011099894
1106	80-81	0.3956215644735792
1106	82-83	0.43051235695649837
1106	84-85	0.45552052569764356
1106	86-87	0.36123040422246433
1106	88-89	0.4607537272868676
1106	90-91	0.4878594148510622
1106	92-93	0.49611992837156293
1106	94-95	0.5199072818881305
1106	96-97	0.5937896419481916
1106	98-99	0.673239983343624
1106	100-101	0.8188828538125215
1107	1	0.04707481912016931
1107	2	0.24104338846300521
1107	3	0.1600505155973977
1107	4	0.1613879466174808
1107	5	0.12821909116016883
1107	6	0.10899645294451688
1107	7	0.24235178894152654
1107	8	0.16228328544353587
1107	9	0.16541542213157356
1107	10-11	0.20511128381765076
1107	12-13	0.27502423593244885
1107	14-15	0.2930612380586197
1107	16-17	0.2673887927451304
1107	18-19	0.20995859891771573
1107	20-21	0.0709598451949418
1107	22-23	0.23582025988984867
1107	24-25	0.275765640408963
1107	26-27	0.26612377951420996
1107	28-29	0.2948042571924887
1107	30-31	0.2333629954191494
1107	32-33	0.342147637133543
1107	34-35	0.25913577715005687
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2314	1	0.11693554681080798
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2314	4	-0.13644942301056062
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2314	96-97	-0.2575528281570918
2314	98-99	-0.2794656986204096
2314	100-101	-0.18057523685411425
2315	1	-0.20406786125959187
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2316	1	-0.2692841644197479
2316	2	-0.4028357708757788
2316	3	-0.6002997833098291
2316	4	-0.36759885793615865
2316	5	-0.47204383486599255
2316	6	-1.0131755126118236
2316	7	-0.6132600493993365
2316	8	-0.4826990047860775
2316	9	-0.571597120387672
2316	10-11	-1.070435805917704
2316	12-13	-0.8743898848450229
2316	14-15	-0.9840804330837614
2316	16-17	-0.8427439515496573
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2316	20-21	-0.6664011006366763
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2316	26-27	-0.4584901685535172
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2316	30-31	-0.389923509700715
2316	32-33	-0.7501752914902937
2316	34-35	-1.2389124622284626
2316	36-37	-0.7518976058037268
2316	38-39	-0.3769693727111232
2316	40-41	-0.3950801891261193
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2316	48-49	-0.4297941389549109
2316	50-51	-0.5450242608258904
2316	52-53	-0.6814837581002564
2316	54-55	-0.5670161445268818
2316	56-57	-0.57550212995001
2316	58-59	-0.5584039754806795
2316	60-61	-0.5846573562141799
2316	62-63	-0.5646833935396884
2316	64-65	-0.6797604376615425
2316	66-67	-0.4915947274236032
2316	68-69	-0.497914856110647
2316	70-71	-0.5602352167115967
2316	72-73	-0.4490719354821451
2316	74-75	-0.4214356397659742
2316	76-77	-0.44658996306318244
2316	78-79	-0.45133040345204734
2316	80-81	-0.41046892089441656
2316	82-83	-0.27409476833278745
2316	84-85	-0.2849301001977871
2316	86-87	-0.26429885843857903
2316	88-89	-0.35354308450367355
2316	90-91	-0.4000380361403977
2316	92-93	-0.4836906095732658
2316	94-95	-0.4655041264236317
2316	96-97	-0.38257941191080036
2316	98-99	-0.28755667926419903
2316	100-101	-0.2980962901743389
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9319.0
3	1376.0
4	1154.0
5	1262.0
6	1512.0
7	1776.0
8	2240.0
9	2807.0
10	3333.0
11	4255.0
12	5361.0
13	6904.0
14	8190.0
15	9923.0
16	11290.0
17	15640.0
18	15114.0
19	9510.0
20	8396.0
21	8454.0
22	9030.0
23	9576.0
24	10924.0
25	12633.0
26	14521.0
27	17464.0
28	20840.0
29	25414.0
30	30948.0
31	39292.0
32	50734.0
33	68048.0
34	97172.0
35	152231.0
36	261943.0
37	515884.0
38	747615.0
39	255879.0
40	387.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58374467687975	19.68285463291519	14.03380422278894	23.69959646741611
2	16.453461831093076	21.806137458787724	38.500552878518896	23.239847831600304
3	18.531903938221305	25.858020506066246	30.976340808565013	24.633734747147436
4	13.768915139740336	19.03719646100434	37.065517840245285	30.128370559010044
5	14.229156481325578	36.75458010698919	34.60221889980058	14.414044511884644
6	29.758149401812712	36.75289460181352	18.776164989768247	14.712791006605514
7	25.434240005451958	33.17407447521853	23.2857868973689	18.105898621960602
8	24.392563773502864	34.11304414133925	21.758758260175448	19.735633824982436
9	25.382704432488556	16.760797308505825	21.746550965405216	36.109947293600406
10-11	23.75102270480709	26.522864485910247	29.27868769357889	20.447425115703776
12-13	24.91981969521141	25.265060451102688	28.340345772113924	21.474774081571976
14-15	22.448943989461497	27.041099386112798	26.606430814812892	23.903525809612812
16-17	21.558393817029785	29.212245838635127	27.584440487937883	21.644919856397205
18-19	21.394505857658167	27.864373440846972	29.700453953546496	21.040666747948364
20-21	22.31394933709816	27.189523392239913	29.48188987877903	21.0146373918829
22-23	22.147358872963355	27.21540117902676	29.049598309834472	21.587641638175413
24-25	21.799347676236014	27.517026510108423	29.34244198980853	21.341183823847032
26-27	21.622306789336758	27.658530214175098	29.519957685795944	21.199205310692207
28-29	21.492844750619916	27.557698946112225	29.656286798961407	21.293169504306455
30-31	21.974670514091102	27.29937451975638	29.45518547518186	21.270769490970658
32-33	21.74265023493696	27.525729501065683	29.22206156423199	21.509558699765368
34-35	21.907187168183327	27.681504671674535	29.144251452006948	21.267056708135193
36-37	21.81339384794689	27.66689674714345	29.268325828842435	21.251383576067223
38-39	21.604143834421848	27.767389754227022	29.395046575751405	21.233419835599726
40-41	21.83921170325217	27.608801289544836	29.286341257241315	21.26564574996167
42-43	21.859943500418776	27.702782999831676	28.908945631827965	21.528327867921583
44-45	21.900519010788315	27.897235539379345	28.75761539004509	21.44463005978725
46-47	21.878112192472283	27.94810196571908	28.689612563856357	21.48417327795228
48-49	21.744530958069767	28.185603575553902	28.67052346761541	21.399341998760924
50-51	21.792778470334483	28.203529997351023	28.520393282060247	21.483298250254247
52-53	21.96574719212434	28.30635559509645	28.230124227318328	21.49777298546088
54-55	21.945191973688775	28.31200517659073	28.16827407703768	21.574528772682818
56-57	21.804711663101344	28.53738296242405	28.246897133901555	21.411008240573054
58-59	21.74699209336349	28.54706047815408	28.35575747411082	21.350189954371608
60-61	21.794704355874554	28.405113287457546	28.52624500202393	21.27393735464397
62-63	21.70190216101748	28.481045517285015	28.39678128900136	21.420271032696142
64-65	21.782608413430722	28.42993432636244	28.267880122486932	21.519577137719907
66-67	21.65940949590045	28.623129321241564	28.231814073637768	21.485647109220217
68-69	21.526174229823695	28.684899108323663	28.377071991302298	21.41185467055034
70-71	21.606218982661726	28.48545138529745	28.42675801253775	21.48157161950307
72-73	21.585801855184016	28.56515291395639	28.304739204999947	21.54430602585965
74-75	21.65875059895193	28.630196180455926	28.130838070368004	21.580215150224138
76-77	21.5932041054304	28.753428139045585	28.090548102665373	21.562819652858643
78-79	21.559974775518135	28.72783704936035	28.18803394879365	21.52415422632786
80-81	21.42994925680293	28.845368641203184	28.15226163912068	21.57242046287321
82-83	21.55337088053357	28.83955620872673	28.11749778641911	21.489575124320584
84-85	21.598703201592766	28.7824503843177	28.043042446133054	21.57580396795649
86-87	21.480698940141064	28.890400780395666	28.06493235942097	21.563967920042305
88-89	21.470690837109117	28.908456274566273	28.039078883854508	21.5817740044701
90-91	21.42011601476817	28.917810769925406	28.18911810845381	21.472955106852613
92-93	21.458118928840733	28.80513073627745	28.21383585790503	21.522914476976787
94-95	21.45774918544644	28.774159004505524	28.165512821422883	21.60257898862515
96-97	21.431545084873484	28.778202696638417	28.168213523180746	21.622038695307356
98-99	21.32969881593604	28.869835296904206	28.023516209016115	21.77694967814364
100-101	21.363134866097205	28.875337450297977	28.14693579101434	21.61459189259048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3932.0
1	2271.5
2	629.5
3	600.0
4	488.5
5	471.0
6	709.0
7	1424.5
8	2384.5
9	2991.5
10	2760.0
11	2201.5
12	2157.0
13	2324.0
14	2442.0
15	2655.0
16	2878.0
17	3100.0
18	3342.0
19	3651.5
20	4146.5
21	4734.5
22	5666.5
23	7050.5
24	8734.0
25	11023.0
26	14316.5
27	18704.0
28	23929.5
29	30032.0
30	37124.5
31	44580.0
32	51359.5
33	57365.5
34	62976.5
35	68063.0
36	73763.0
37	78468.0
38	80913.0
39	82191.5
40	83174.5
41	84509.0
42	85860.5
43	88126.5
44	90957.0
45	93175.5
46	93429.5
47	92786.5
48	92984.0
49	91362.5
50	86904.5
51	81759.5
52	77108.0
53	73086.0
54	68437.0
55	62860.0
56	56306.0
57	49179.0
58	43110.5
59	36776.0
60	29801.5
61	24034.0
62	18999.5
63	14305.5
64	10498.5
65	7724.5
66	5889.5
67	4477.5
68	3475.5
69	2718.5
70	2083.0
71	1520.0
72	1065.0
73	834.0
74	618.0
75	443.0
76	357.5
77	272.0
78	181.5
79	120.0
80	86.5
81	71.0
82	53.0
83	42.5
84	37.5
85	28.0
86	21.5
87	19.5
88	18.0
89	16.0
90	12.0
91	11.5
92	6.5
93	5.0
94	5.5
95	4.5
96	4.0
97	1.5
98	1.5
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15662278176807107
2	0.1610791982177575
3	0.1544350864200432
4	0.15893201574654495
5	0.14649456256423823
6	0.12097145017057948
7	0.1289119740263844
8	0.11777093290216828
9	0.11367913234381982
10-11	0.09980347203456884
12-13	0.12032324414153416
14-15	0.14116711926302217
16-17	0.1529361099778759
18-19	0.14874302722748914
20-21	0.1389996803534019
22-23	0.13235556855568759
24-25	0.1157655454998094
26-27	0.12093093729376413
28-29	0.1255088923738966
30-31	0.11365887590541217
32-33	0.11327400357566651
34-35	0.13079582279829732
36-37	0.1325378765013566
38-39	0.11768990714853764
40-41	0.12093093729376413
42-43	0.11529964741643307
44-45	0.1327606973238409
46-47	0.1315858238961963
48-49	0.14641353681060756
50-51	0.13166684964982694
52-53	0.11467169782579544
54-55	0.10677168684680581
56-57	0.1284460759430081
58-59	0.11710247043471532
60-61	0.1155832375541404
62-63	0.11716323974993832
64-65	0.13916173186066325
66-67	0.12976274443950636
68-69	0.13494839267186878
70-71	0.12137657893873277
72-73	0.12328068414905337
74-75	0.10474604300603925
76-77	0.13265941513180257
78-79	0.1331050567767712
80-81	0.13334813403766319
82-83	0.1399112200817469
84-85	0.12976274443950636
86-87	0.1396681428208549
88-89	0.12482017346803595
90-91	0.13450275102690015
92-93	0.14890507873475045
94-95	0.12330094058746102
96-97	0.10741989287585113
98-99	0.11070143589789296
100-101	0.15856739985520696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2468351.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.067580884775936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.27531853452678	37.2510452024862
2	20.081050917669714	22.91953994990841
3	7.579169047231904	12.975745279268905
4	3.1186534890501325	7.118960409518284
5	1.5180862216118294	4.331675412094848
6	0.7773352249341002	2.6616384494107357
7	0.4444569248608178	1.7754857056505453
8	0.297501991858783	1.358217518702644
9	0.18799164499800708	0.9655405565927268
>10	0.6961976039466107	6.383556840272885
>50	0.01838727535062516	0.6978641945667885
>100	0.005282113073244263	0.5465869940626047
>500	2.133790827941026E-4	0.09510258919900377
>1k	2.133790827929876E-4	0.2850236369410594
>5k	1.4225272186199172E-4	0.6340172613243161
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7854	0.3181881345076126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7766	0.3146230013478634	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3570	0.14463097023073299	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.051287681533137E-5	0.0
2	0.0	0.0	0.0	4.051287681533137E-5	0.0
3	0.0	0.0	0.0	4.051287681533137E-5	0.0
4	0.0	0.0	0.0	8.102575363066274E-5	0.0
5	0.0	0.0	0.0	8.102575363066274E-5	0.0
6	0.0	0.0	0.0	8.102575363066274E-5	0.0
7	0.0	0.0	0.0	8.102575363066274E-5	0.0
8	0.0	0.0	0.0	8.102575363066274E-5	4.051287681533137E-5
9	0.0	0.0	0.0	8.102575363066274E-5	8.102575363066274E-5
10-11	0.0	1.6205150726132547E-4	0.0	8.102575363066274E-5	8.102575363066274E-5
12-13	0.0	1.6205150726132547E-4	0.0	1.0128219203832842E-4	8.102575363066274E-5
14-15	0.0	1.6205150726132547E-4	0.0	1.215386304459941E-4	8.102575363066274E-5
16-17	0.0	1.6205150726132547E-4	0.0	1.6205150726132547E-4	8.102575363066274E-5
18-19	0.0	1.6205150726132547E-4	0.0	1.6205150726132547E-4	8.102575363066274E-5
20-21	0.0	2.0256438407665684E-4	0.0	2.2282082248432252E-4	8.102575363066274E-5
22-23	0.0	2.835901377073196E-4	0.0	3.2410301452265094E-4	8.102575363066274E-5
24-25	0.0	3.0384657611498523E-4	0.0	3.646158913379823E-4	8.102575363066274E-5
26-27	0.0	3.2410301452265094E-4	0.0	6.279495906376363E-4	8.102575363066274E-5
28-29	0.0	3.2410301452265094E-4	0.0	0.0011748734276446097	8.102575363066274E-5
30-31	4.051287681533137E-5	3.2410301452265094E-4	0.0	0.0021876953480278937	8.102575363066274E-5
32-33	4.051287681533137E-5	3.646158913379823E-4	0.0	0.004091800558348469	8.102575363066274E-5
34-35	4.051287681533137E-5	3.8487232974564797E-4	0.0	0.006846676181791002	8.102575363066274E-5
36-37	4.051287681533137E-5	4.4564164496864504E-4	0.0	0.009277448790710883	8.102575363066274E-5
38-39	4.051287681533137E-5	4.861545217839764E-4	0.0	0.01079668167128581	1.215386304459941E-4
40-41	4.051287681533137E-5	4.861545217839764E-4	0.0	0.012275401675045405	1.215386304459941E-4
42-43	4.051287681533137E-5	5.469238370069734E-4	0.0	0.015718996204348572	1.215386304459941E-4
44-45	4.051287681533137E-5	6.076931522299706E-4	0.0	0.01938541155613606	1.215386304459941E-4
46-47	4.051287681533137E-5	6.076931522299706E-4	0.0	0.02262644170136257	1.215386304459941E-4
48-49	4.051287681533137E-5	6.076931522299706E-4	0.0	0.030647991310798182	1.215386304459941E-4
50-51	4.051287681533137E-5	6.076931522299706E-4	0.0	0.03650210201061356	1.215386304459941E-4
52-53	4.051287681533137E-5	6.076931522299706E-4	0.0	0.04571878148610145	1.6205150726132547E-4
54-55	4.051287681533137E-5	6.076931522299706E-4	0.0	0.05937162097286812	2.0256438407665684E-4
56-57	4.051287681533137E-5	6.076931522299706E-4	0.0	0.07879754540581951	2.0256438407665684E-4
58-59	4.051287681533137E-5	6.482060290453019E-4	0.0	0.09676500627341897	2.430772608919882E-4
60-61	4.051287681533137E-5	6.887189058606333E-4	0.0	0.11937119153637388	2.835901377073196E-4
62-63	4.051287681533137E-5	6.887189058606333E-4	0.0	0.15178149298863897	2.835901377073196E-4
64-65	4.051287681533137E-5	7.292317826759646E-4	0.0	0.1882430821224372	2.835901377073196E-4
66-67	4.051287681533137E-5	7.292317826759646E-4	0.0	0.24340136390651088	2.835901377073196E-4
68-69	4.051287681533137E-5	7.292317826759646E-4	0.0	0.3125568446302815	2.835901377073196E-4
70-71	4.051287681533137E-5	7.292317826759646E-4	0.0	0.3756151373933448	2.835901377073196E-4
72-73	4.051287681533137E-5	7.292317826759646E-4	0.0	0.44882190579864856	3.646158913379823E-4
74-75	4.051287681533137E-5	7.292317826759646E-4	0.0	0.5316909953244089	3.646158913379823E-4
76-77	4.051287681533137E-5	7.292317826759646E-4	0.0	0.6269165122788453	3.8487232974564797E-4
78-79	4.051287681533137E-5	7.292317826759646E-4	0.0	0.7279758834946894	4.051287681533137E-4
80-81	4.051287681533137E-5	7.292317826759646E-4	0.0	0.8302911538918087	4.051287681533137E-4
82-83	4.051287681533137E-5	7.292317826759646E-4	0.0	0.9448210566487505	4.051287681533137E-4
84-85	4.051287681533137E-5	7.292317826759646E-4	0.0	1.0693576399790792	4.051287681533137E-4
86-87	4.051287681533137E-5	7.292317826759646E-4	0.0	1.2249068305115438	4.051287681533137E-4
88-89	4.051287681533137E-5	7.292317826759646E-4	0.0	1.3894903925738276	4.051287681533137E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4440	0.0	57.676216	1
GTATCAA	7585	0.0	38.2716	1
TCAACGC	9015	0.0	31.619783	4
ATCAACG	9070	0.0	31.428043	3
TATCAAC	9325	0.0	30.977457	2
CAACGCA	9240	0.0	30.950142	5
GTGGTAT	1840	0.0	30.727	1
TGGTATC	1800	0.0	30.617975	2
AACGCAG	9455	0.0	30.340073	6
ACGCAGA	10725	0.0	26.570759	7
CGCAGAG	10960	0.0	25.787504	8
GAGTACT	7700	0.0	23.785213	12-13
GCAGAGT	12090	0.0	22.984362	9
CAGAGTA	11515	0.0	22.07108	10-11
GTACTTT	8560	0.0	21.035248	14-15
AGAGTAC	10535	0.0	19.705189	10-11
GTACATG	5650	0.0	19.34062	1
TACATGG	5865	0.0	18.064579	2
AGTACTT	8630	0.0	17.203333	12-13
ACTTTTT	10695	0.0	17.035965	16-17
>>END_MODULE