FastQCFastQC Report
Fri 10 Feb 2017
SRR2031701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468351
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110880.44920677812839416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106390.43101649643831047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.20341515448977882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.12145760469236344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.062.3163531
GTATCAA90750.039.5788841
TGGTATC19750.033.4322552
GTGGTAT20500.033.3731351
TCAACGC108200.033.0141224
ATCAACG108400.032.9538763
CAACGCA109750.032.587185
TATCAAC111050.032.210272
AACGCAG113600.031.5245886
ACGCAGA125900.028.3692917
CGCAGAG128150.027.8711978
GCAGAGT139900.025.0550379
GAGTACT106600.024.5737312-13
CAGAGTA136750.023.68862310-11
GTACTTT116650.022.23262214-15
ACGGCGT1404.8567136E-420.3553988
AGAGTAC128050.020.10492710-11
GTACATG47350.018.964011
AGTACTT115200.018.8222312-13
ACTTTTT139550.018.61774616-17
Produced by FastQC (version 0.11.5)