FastQCFastQC Report
Fri 10 Feb 2017
SRR2031700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391175
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167140.3100251800396018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157250.2916803850737548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.13247575899502428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105550.056.2605931
GTATCAA182300.039.8188971
TCAACGC218700.033.016824
ATCAACG220200.032.7271843
CAACGCA222950.032.365525
TATCAAC224600.032.19272
AACGCAG232150.031.1583776
GTGGTAT51200.030.4338131
TGGTATC52650.029.1445032
ACGCAGA257400.027.9555387
CGCAGAG262200.027.2105488
GCAGAGT290600.023.8320839
GAGTACT161550.023.5249912-13
CAGAGTA271200.022.32422610-11
GTACTTT175450.021.41803614-15
GTACATG163000.021.0427321
TACATGG161100.020.9075532
ACATGGG164250.019.6672633
AGAGTAC248950.019.6349410-11
AGTACTT175300.017.57415812-13
Produced by FastQC (version 0.11.5)