FastQCFastQC Report
Fri 10 Feb 2017
SRR2031700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391175
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210040.3895996698307883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205630.38141963486623975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95420.17699295608100274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57110.10593238023250962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117950.055.5126081
GTATCAA198700.038.3811071
TCAACGC243150.031.162924
ATCAACG244900.030.9211253
CAACGCA245950.030.8597935
TATCAAC248950.030.6470832
AACGCAG255050.029.8146086
GTGGTAT56950.026.6990991
ACGCAGA286650.026.4450287
CGCAGAG290950.025.907278
TGGTATC58300.025.4238762
GAGTACT192250.023.31045312-13
GCAGAGT318150.023.1549039
CAGAGTA305350.021.34181410-11
GTACTTT208750.021.12664814-15
GTACATG158700.019.7609271
AGAGTAC276300.019.39114210-11
TACATGG156700.019.3725852
ACATGGG159500.018.1687413
AGTACTT208450.018.02394112-13
Produced by FastQC (version 0.11.5)