FastQCFastQC Report
Fri 10 Feb 2017
SRR2031699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907721
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.26373742592712957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.2517293309287766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.1153438115896845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.11247949535154525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.059.417931
GTATCAA23500.036.181441
TCAACGC27450.030.9732955
ATCAACG27650.030.9210384
CAACGCA27550.030.6867716
TATCAAC28650.029.8434183
AACGCAG28550.029.6135587
GTGGTAT6600.026.629221
TGGTATC6650.025.7147032
ACGCAGA33400.025.316188
CGCAGAG34700.024.2308379
CAGAGTA35800.021.297510-11
GAGTACT23450.021.1700612-13
GCAGAGT39000.019.8539839
GAACCGC1250.00603345918.998216
GTACTTT26700.018.6821414-15
GCGTCAG2654.0029954E-717.924821
AGAGTAC32550.017.44028310-11
GTACATG20750.017.3978731
CTATTAG3101.1154589E-716.8551121
Produced by FastQC (version 0.11.5)