FastQCFastQC Report
Fri 10 Feb 2017
SRR2031699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907721
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.34459927664998385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.3087953236732432No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT18900.20821375731089178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.14596996213594265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.058.547371
GTATCAA26000.038.7387241
CCATACG500.001615756337.9994282
GTGGTAT6800.037.029661
TGGTATC7250.032.7581252
TCAACGC31200.032.2751544
ATCAACG31550.031.917113
CAACGCA31750.031.8639075
TATCAAC32400.031.5195882
AACGCAG33300.030.5233846
CGTACGG350.00838555427.14768476-77
ACGCAGA37400.027.0502417
CGCAGAG37850.026.4791248
GCAGAGT42300.022.9074559
GAGTACT30250.021.9843512-13
TACATGG17700.021.736962
GTACATG18500.021.3151871
CAGAGTA41150.021.24021710-11
GTACTTT34450.019.09675414-15
AGAGTAC38050.018.47642910-11
Produced by FastQC (version 0.11.5)