##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031699_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 907721 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7184784752143 34.0 31.0 34.0 31.0 34.0 2 32.88499549971853 34.0 31.0 34.0 31.0 34.0 3 32.9409994921347 34.0 31.0 34.0 31.0 34.0 4 36.343601172607 37.0 37.0 37.0 35.0 37.0 5 36.30479850086095 37.0 37.0 37.0 35.0 37.0 6 36.334174267203245 37.0 37.0 37.0 35.0 37.0 7 36.28372925160925 37.0 37.0 37.0 35.0 37.0 8 36.2768967557212 37.0 37.0 37.0 35.0 37.0 9 38.124153787342145 39.0 39.0 39.0 37.0 39.0 10-11 38.08422742230267 39.0 39.0 39.0 36.0 39.0 12-13 38.032735278791606 39.0 39.0 39.0 35.0 39.0 14-15 39.51099622020423 41.0 39.0 41.0 36.5 41.0 16-17 39.48043011013296 41.0 39.0 41.0 36.5 41.0 18-19 39.48750166626089 41.0 39.0 41.0 36.5 41.0 20-21 39.48057883424532 41.0 39.0 41.0 36.5 41.0 22-23 39.386632015784585 41.0 39.0 41.0 36.0 41.0 24-25 39.3158106951365 41.0 39.0 41.0 36.0 41.0 26-27 39.17709626636378 41.0 39.0 41.0 36.0 41.0 28-29 39.07361127483004 40.0 39.0 41.0 35.5 41.0 30-31 38.897536247371164 40.0 38.5 41.0 35.0 41.0 32-33 38.6788236693874 40.0 38.0 41.0 35.0 41.0 34-35 38.533621013505254 40.0 38.0 41.0 35.0 41.0 36-37 38.4275030543526 40.0 38.0 41.0 34.5 41.0 38-39 38.49905863145173 40.0 38.0 41.0 35.0 41.0 40-41 38.425610953145295 40.0 38.0 41.0 34.5 41.0 42-43 38.31727425056818 40.0 38.0 41.0 34.0 41.0 44-45 38.11864658854428 40.0 38.0 41.0 34.0 41.0 46-47 37.89193705995565 40.0 38.0 41.0 33.0 41.0 48-49 37.6279600229586 40.0 38.0 41.0 33.0 41.0 50-51 37.460470232593494 40.0 37.0 41.0 33.0 41.0 52-53 37.51646596255898 40.0 37.0 41.0 33.0 41.0 54-55 37.43948801448903 40.0 37.0 41.0 33.0 41.0 56-57 37.24155660164301 40.0 37.0 41.0 33.0 41.0 58-59 37.050441710613725 40.0 36.0 41.0 32.0 41.0 60-61 36.77419383268648 39.0 36.0 41.0 32.0 41.0 62-63 36.48821058452983 39.0 35.0 41.0 31.0 41.0 64-65 36.147967271881996 39.0 35.0 41.0 31.0 41.0 66-67 35.77916452301974 38.0 35.0 40.0 31.0 41.0 68-69 35.37798839070595 37.0 35.0 40.0 30.0 41.0 70-71 35.012837094217275 37.0 35.0 39.0 30.0 41.0 72-73 34.62035911915666 36.0 35.0 39.0 29.5 41.0 74-75 34.235621407899565 36.0 34.0 39.0 29.0 40.0 76-77 33.30934615371904 35.0 33.5 37.0 28.0 39.0 78-79 33.51649130074109 35.0 34.0 37.0 29.0 39.0 80-81 33.2992626589007 35.0 34.0 36.5 29.0 39.0 82-83 33.03428035707007 35.0 34.0 36.0 29.0 37.5 84-85 32.745273051962 35.0 34.0 36.0 29.0 37.0 86-87 32.50000771162064 35.0 34.0 35.5 28.0 37.0 88-89 32.27706475888516 35.0 34.0 35.0 27.0 36.0 90-91 32.08000861498192 35.0 34.0 35.0 27.0 36.0 92-93 31.911158825233745 35.0 33.5 35.0 26.0 36.0 94-95 31.73349355143265 35.0 33.0 35.0 26.0 36.0 96-97 31.562643697788197 35.0 33.0 35.0 25.0 35.0 98-99 31.34254137559889 35.0 33.0 35.0 25.0 35.0 100-101 30.61758348655589 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23463394183212216 1101 2 0.26650794517384924 1101 3 0.2082857956101023 1101 4 0.18252833903500232 1101 5 0.10769153542724297 1101 6 0.15408891061373708 1101 7 0.19197783327069118 1101 8 0.22114518861579313 1101 9 0.1460685645019808 1101 10-11 0.21605067611742612 1101 12-13 0.22113610323687993 1101 14-15 0.3013228257101872 1101 16-17 0.145806837677938 1101 18-19 0.2897758477575607 1101 20-21 0.2432789618489437 1101 22-23 0.0965464106809506 1101 24-25 0.1320698463968455 1101 26-27 0.1831189027850897 1101 28-29 0.21079084973348472 1101 30-31 0.17499371767804206 1101 32-33 0.1642966151760703 1101 34-35 -0.1315521944821043 1101 36-37 -0.029428310488626153 1101 38-39 -0.024838353859827578 1101 40-41 -0.15459109912641367 1101 42-43 -0.008648008921952055 1101 44-45 -0.04713284416563113 1101 46-47 0.04617620813316847 1101 48-49 -0.16219262606895768 1101 50-51 -0.31849177479417534 1101 52-53 -0.2660372212034545 1101 54-55 -0.3451773332276389 1101 56-57 -0.3007426139142453 1101 58-59 -0.2539695664534847 1101 60-61 -0.23339006409187135 1101 62-63 -0.25909314780594883 1101 64-65 -0.2985495936849176 1101 66-67 -0.6910307582381634 1101 68-69 -0.5390902864252354 1101 70-71 -0.4213818566313421 1101 72-73 -0.6701539890164767 1101 74-75 -0.5749480047608273 1101 76-77 -0.49882704301647607 1101 78-79 -0.466168947102247 1101 80-81 -0.5361914993651453 1101 82-83 -0.45349640122783086 1101 84-85 -0.5130958793318143 1101 86-87 -0.4671657242814078 1101 88-89 -0.6642148492806292 1101 90-91 -0.6000202035173956 1101 92-93 -0.7156374647850328 1101 94-95 -0.6017378402908768 1101 96-97 -0.6604264597221388 1101 98-99 -0.83323115278721 1101 100-101 -1.0670811129491895 1102 1 0.10915378818295096 1102 2 0.04964291995047887 1102 3 0.12031884837277573 1102 4 0.012092160113482464 1102 5 0.04953434589162242 1102 6 0.0744866072755741 1102 7 0.095901624402984 1102 8 0.014182348485093144 1102 9 0.0017321036104505083 1102 10-11 0.027743860559404254 1102 12-13 0.05960244525321201 1102 14-15 0.043442708444246136 1102 16-17 0.05699065693725913 1102 18-19 0.0629762773595175 1102 20-21 0.11077404661486412 1102 22-23 0.06871236717500295 1102 24-25 0.10526695253091845 1102 26-27 0.26308241460613857 1102 28-29 0.23460612089348842 1102 30-31 -0.14868669065371165 1102 32-33 0.1495579097192845 1102 34-35 0.09974630191106115 1102 36-37 0.1644811722398387 1102 38-39 0.1332870003518778 1102 40-41 0.35335023991628844 1102 42-43 0.22809572721568827 1102 44-45 0.33781307076758793 1102 46-47 0.4268916982922093 1102 48-49 0.36405052583801023 1102 50-51 0.32465261064414364 1102 52-53 0.30114843121078394 1102 54-55 0.46946411706377944 1102 56-57 0.493365927048373 1102 58-59 0.3288276390347846 1102 60-61 0.4554896123772849 1102 62-63 0.4686606077127564 1102 64-65 0.3275488999095373 1102 66-67 0.3271153763937704 1102 68-69 0.3223971928468288 1102 70-71 0.40175630167767906 1102 72-73 0.6340264088201195 1102 74-75 0.25632827687333304 1102 76-77 0.31465556650054083 1102 78-79 0.3162234949819478 1102 80-81 0.19249982393482412 1102 82-83 0.32474957737603205 1102 84-85 0.5095430591175614 1102 86-87 0.5626081749510448 1102 88-89 0.535232580454263 1102 90-91 0.2960925634970053 1102 92-93 0.3327032501453857 1102 94-95 0.3626686932971346 1102 96-97 0.16618209873240986 1102 98-99 0.13086664812657034 1102 100-101 0.19952573129561202 1103 1 0.2990701252274164 1103 2 0.25368977628279765 1103 3 0.2624125959432462 1103 4 0.2265235042898368 1103 5 0.19841410637408075 1103 6 0.15963464770923252 1103 7 0.27956188267447146 1103 8 0.23323713003005508 1103 9 0.19744281034267885 1103 10-11 0.2676509158232818 1103 12-13 0.22402323834567284 1103 14-15 0.3191179381484872 1103 16-17 0.3124252584632856 1103 18-19 0.4515798374380253 1103 20-21 0.44169649694147495 1103 22-23 0.3705296922134451 1103 24-25 0.2090245663675887 1103 26-27 0.3377825020294054 1103 28-29 0.3729935027491109 1103 30-31 0.4248083519234811 1103 32-33 0.2540136403858071 1103 34-35 0.2991133955396279 1103 36-37 0.14868786559360814 1103 38-39 0.0990316768143984 1103 40-41 0.39723005593077687 1103 42-43 0.44838014635868007 1103 44-45 0.4374208560395374 1103 46-47 0.5728790338182606 1103 48-49 0.54346179122237 1103 50-51 0.43108924781537183 1103 52-53 0.5053357042236541 1103 54-55 0.7980875417393491 1103 56-57 0.6627504894639813 1103 58-59 0.5660199718080392 1103 60-61 0.5208047883265223 1103 62-63 0.3975129829759325 1103 64-65 0.6749278678707427 1103 66-67 0.5308393536922793 1103 68-69 0.5094171383619752 1103 70-71 0.5985538493836486 1103 72-73 0.6939525988316575 1103 74-75 0.7801213677380048 1103 76-77 0.7839725385139857 1103 78-79 0.6716085936782221 1103 80-81 0.5988411046936051 1103 82-83 0.5964304681731036 1103 84-85 0.6317357171497875 1103 86-87 0.5390681514371991 1103 88-89 0.5838223051265032 1103 90-91 0.5276460162400483 1103 92-93 0.6397366185432247 1103 94-95 0.6399765644057318 1103 96-97 0.6140240450821359 1103 98-99 0.6805773262994741 1103 100-101 0.34553289968615175 1104 1 0.07498047648558526 1104 2 0.07401349081037978 1104 3 0.1866307612542073 1104 4 0.07231895744110517 1104 5 0.16186969914525662 1104 6 0.02037820347744912 1104 7 0.1303062972646316 1104 8 0.168218174602913 1104 9 -0.08269570536762672 1104 10-11 0.09637651607847175 1104 12-13 0.10113732057173053 1104 14-15 0.1379632520479035 1104 16-17 0.20310949417309843 1104 18-19 0.1539550935509979 1104 20-21 0.1651328275633901 1104 22-23 0.14924348366694318 1104 24-25 0.09816368938770381 1104 26-27 0.20156490722159504 1104 28-29 0.14713100014197522 1104 30-31 0.018386970937804392 1104 32-33 0.10428877012926563 1104 34-35 0.020181831379495918 1104 36-37 0.1596677507568458 1104 38-39 0.06915004088128995 1104 40-41 0.016816410477012766 1104 42-43 -0.003112516848155167 1104 44-45 0.075287082253233 1104 46-47 0.1471372263661479 1104 48-49 -0.02241215211992653 1104 50-51 0.04317164564623255 1104 52-53 0.15622918576511324 1104 54-55 0.06689727587261274 1104 56-57 0.14608014213185783 1104 58-59 -0.014009197465767897 1104 60-61 0.2072580990851023 1104 62-63 0.24597231775700124 1104 64-65 0.06429396526115028 1104 66-67 -0.03829581261245352 1104 68-69 0.14439250857557795 1104 70-71 0.07545307276323854 1104 72-73 0.028887021641963884 1104 74-75 0.1265885423365063 1104 76-77 0.02885718032388951 1104 78-79 0.010434553411741376 1104 80-81 -0.0019370493299746272 1104 82-83 -0.14634929307440103 1104 84-85 -0.15837126752637687 1104 86-87 -0.025996812308243022 1104 88-89 -0.18524430878395748 1104 90-91 -0.28750329608894987 1104 92-93 -0.10665556249644226 1104 94-95 -0.046781934864270625 1104 96-97 -0.11827962208755594 1104 98-99 -0.1431172643491827 1104 100-101 0.09184533297409203 1105 1 0.12524036275959105 1105 2 0.11330105511972732 1105 3 0.023555803342240722 1105 4 0.08228175514774705 1105 5 0.07124650186714376 1105 6 0.037573962886916945 1105 7 0.07819512598103273 1105 8 0.015400336338707632 1105 9 -0.051029607723378945 1105 10-11 -0.020399393081994788 1105 12-13 0.07251083277432002 1105 14-15 0.03768923832073057 1105 16-17 -0.1157824425830043 1105 18-19 -0.15109193909518837 1105 20-21 0.004410973570614374 1105 22-23 0.1378605825944348 1105 24-25 0.15067734592577153 1105 26-27 -0.0399461121918776 1105 28-29 0.17930185991522762 1105 30-31 -0.0023119553757595668 1105 32-33 -0.35804537945691095 1105 34-35 -0.22919567771504745 1105 36-37 -0.34757919123818937 1105 38-39 -0.3152469228935715 1105 40-41 -0.1589217489513004 1105 42-43 -0.22035522060723878 1105 44-45 -0.2368229411747862 1105 46-47 -0.18274395586622916 1105 48-49 -0.390175511392826 1105 50-51 -0.2792227222091057 1105 52-53 -0.15239827329470756 1105 54-55 -0.21173289094906522 1105 56-57 -0.3835845663128339 1105 58-59 -0.2723407158137974 1105 60-61 -0.3029224968579669 1105 62-63 -0.6056084853887143 1105 64-65 -0.6584069388958582 1105 66-67 -0.4926817981789071 1105 68-69 -0.53928277779967 1105 70-71 -0.41051218547482904 1105 72-73 -0.42754744463462657 1105 74-75 -0.30650619400989854 1105 76-77 -0.5323522179643305 1105 78-79 -0.3988708762394353 1105 80-81 -0.38341507525385055 1105 82-83 -0.3775833261090966 1105 84-85 -0.33475546361542285 1105 86-87 -0.25874954655907345 1105 88-89 -0.21914798898550458 1105 90-91 -0.37578074749639967 1105 92-93 -0.39282699325426407 1105 94-95 -0.4012000015223016 1105 96-97 -0.4678384334978283 1105 98-99 -0.4235104195981201 1105 100-101 -0.3985869965252071 1106 1 0.20130060849879072 1106 2 0.19906063838228505 1106 3 0.2235473100519414 1106 4 0.22329726955803153 1106 5 0.1252949702437931 1106 6 0.2368562798565108 1106 7 0.14533458580777392 1106 8 0.2467306687094677 1106 9 0.28123640212883316 1106 10-11 0.28924544582859113 1106 12-13 0.21255881986451897 1106 14-15 0.33839347364149575 1106 16-17 0.3221924738996407 1106 18-19 0.34459630052884194 1106 20-21 0.36813259182552116 1106 22-23 0.37067794854199576 1106 24-25 0.3358169189963718 1106 26-27 0.27039446874917417 1106 28-29 0.2522818723720519 1106 30-31 0.2815706107303484 1106 32-33 0.3822708540519457 1106 34-35 0.4408619351041594 1106 36-37 0.3775779346323773 1106 38-39 0.36300317619481604 1106 40-41 0.3831235769079413 1106 42-43 0.42028446570724753 1106 44-45 0.4797602081372574 1106 46-47 0.40326051726666634 1106 48-49 0.35660128493806553 1106 50-51 0.38295240943689635 1106 52-53 0.46953069448741047 1106 54-55 0.39932891189335606 1106 56-57 0.5966812736735747 1106 58-59 0.4828083414309745 1106 60-61 0.5679370241204609 1106 62-63 0.5544412003595411 1106 64-65 0.6666631307491215 1106 66-67 0.6577202175619945 1106 68-69 0.629040080475626 1106 70-71 0.2861025305661613 1106 72-73 0.6037043248008516 1106 74-75 0.7488127290408926 1106 76-77 0.6204059780608091 1106 78-79 0.4400078678860453 1106 80-81 0.5308069393546049 1106 82-83 0.5405024278119797 1106 84-85 0.5883873369054982 1106 86-87 0.4278869689270266 1106 88-89 0.5899030273782344 1106 90-91 0.6447397496441951 1106 92-93 0.6441400527793704 1106 94-95 0.6486329637684527 1106 96-97 0.7293276386385124 1106 98-99 0.8305861774235659 1106 100-101 0.8558599487213776 1107 1 0.32006107751051616 1107 2 0.25615563718582024 1107 3 0.2857152928229425 1107 4 0.18075718819885367 1107 5 0.1202818092004847 1107 6 0.19821665315156167 1107 7 0.12074736398437125 1107 8 0.10495632077934403 1107 9 0.25632613651504954 1107 10-11 0.27429270593867017 1107 12-13 0.3053172548968277 1107 14-15 0.33432252365345505 1107 16-17 0.2886796717938793 1107 18-19 0.4004350179253393 1107 20-21 0.3965933479872845 1107 22-23 0.45318273437592893 1107 24-25 0.4468781776706976 1107 26-27 0.4316292139035198 1107 28-29 0.5130260695484168 1107 30-31 0.5162320129796925 1107 32-33 0.5219214881749394 1107 34-35 0.4513040265135899 1107 36-37 0.401716484524691 1107 38-39 0.4710015011529407 1107 40-41 0.5029040267763278 1107 42-43 0.5468308882963768 1107 44-45 0.5682562598242598 1107 46-47 0.5131971049670696 1107 48-49 0.4266120530644173 1107 50-51 0.6356264941461589 1107 52-53 0.5283994429918479 1107 54-55 0.44798588724631827 1107 56-57 0.4349440159564253 1107 58-59 0.45207431415172294 1107 60-61 0.6034718410623157 1107 62-63 0.6926052348175418 1107 64-65 0.5396441070592459 1107 66-67 0.48055462770796 1107 68-69 0.5104710448211662 1107 70-71 0.6046087521502699 1107 72-73 0.427122008457232 1107 74-75 0.3876587448341411 1107 76-77 0.4459892515712198 1107 78-79 0.5045418473069603 1107 80-81 0.32649968881619884 1107 82-83 0.2934487068260907 1107 84-85 0.21524184755397613 1107 86-87 0.26745684210242615 1107 88-89 0.4254857026593939 1107 90-91 0.4114991418432865 1107 92-93 0.4650302178906429 1107 94-95 0.4149885512295661 1107 96-97 0.4051232835296368 1107 98-99 0.6171320017834603 1107 100-101 0.6090694486017334 1108 1 0.1065440541916729 1108 2 0.038172462887366976 1108 3 -0.04683792269284481 1108 4 0.025878863993554546 1108 5 0.016600481212229568 1108 6 -0.013504875841718444 1108 7 -0.007715173421765087 1108 8 0.11365148197859298 1108 9 0.05001649843702438 1108 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-0.38762238410884464 2316 62-63 -0.3916001773565867 2316 64-65 -0.47252434035582525 2316 66-67 -0.3967180626044424 2316 68-69 -0.510580816426824 2316 70-71 -0.4010160547320396 2316 72-73 -0.5281438027997041 2316 74-75 -0.5171200032753376 2316 76-77 -0.8338647107972648 2316 78-79 -0.7337958769128434 2316 80-81 -0.4318490939324917 2316 82-83 -0.3989046456970371 2316 84-85 -0.39808977821239466 2316 86-87 -0.3194257911284524 2316 88-89 -0.391176491808082 2316 90-91 -0.4633219701893694 2316 92-93 -0.4305825547102273 2316 94-95 -0.3539329169527683 2316 96-97 -0.3215623289990255 2316 98-99 -0.29992699872067874 2316 100-101 -0.251739450261514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 12.0 6 18.0 7 45.0 8 139.0 9 282.0 10 492.0 11 826.0 12 1262.0 13 1685.0 14 2164.0 15 2643.0 16 3632.0 17 5169.0 18 5387.0 19 3237.0 20 2568.0 21 2567.0 22 2907.0 23 3109.0 24 3594.0 25 4342.0 26 4598.0 27 5105.0 28 6474.0 29 8516.0 30 10682.0 31 13812.0 32 17444.0 33 23852.0 34 33806.0 35 53075.0 36 94777.0 37 202803.0 38 299870.0 39 86721.0 40 103.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.9899048108061 19.55350659625083 14.11012593092916 24.346462662013906 2 16.586374006991132 21.600800245890532 38.12493045770672 23.687895289411614 3 18.87095725823421 25.202405622078487 30.95547351733544 24.971163602351858 4 13.800854372184093 18.89127744838673 36.49482627728862 30.813041902140558 5 14.213618501720243 36.33517347290633 34.7617825300946 14.689425495278835 6 30.19881659672961 36.345639243776446 18.504033728425366 14.951510431068577 7 25.65799403120562 32.402577443950285 23.122192832379113 18.817235692464976 8 24.27519028423932 34.02851757313095 21.846029782278915 19.85026236035081 9 25.27439598731328 16.22668198708634 21.61049485469654 36.88842717090384 10-11 23.8251070538194 26.12372083492615 29.279811748323546 20.771360362930903 12-13 24.71878733596961 24.570118224717852 28.50425874484492 22.206835694467625 14-15 22.28990091112848 26.46078831534597 26.515375602952673 24.73393517057288 16-17 21.56177220377523 28.69535951067857 27.640998296805353 22.101869988740848 18-19 21.570540238476752 27.412983856797172 29.46796890449087 21.548507000235205 20-21 22.176197311729045 26.79072093738054 29.334949835907732 21.69813191498269 22-23 22.08811958740626 26.817656526619967 28.998668092949266 22.09555579302451 24-25 21.917472439218656 26.97602016478632 29.410688967204678 21.695818428790346 26-27 21.755152492629854 27.20272640341252 29.507356926615813 21.53476417734181 28-29 21.617199804111994 27.02747665291521 29.604538696419315 21.75078484655348 30-31 22.124786346442924 26.900105153629504 29.366671091675343 21.608437408252218 32-33 21.947571938483232 27.128189309478696 29.109527166967787 21.814711585070288 34-35 21.98939525403187 27.207150628709943 29.273175501659576 21.530278615598615 36-37 22.06120679492815 26.99390862608615 29.2381541010734 21.7067304779123 38-39 21.63698977989933 27.04327651337801 29.585081759703698 21.734651947018964 40-41 21.940937799169568 26.96891445719555 29.355110215583863 21.73503752805102 42-43 22.03061293062516 27.04889497984513 29.09153803867047 21.828954050859238 44-45 22.015795602393247 27.184619503129266 28.928106764082795 21.871478130394692 46-47 22.036341563101438 27.20191556656726 28.66761923542586 22.094123634905436 48-49 21.944132613435187 27.19888600131538 28.71901167869863 22.137969706550802 50-51 22.00290617932162 27.422137418876506 28.540267328837825 22.03468907296405 52-53 22.00560524654602 27.604131666007508 28.42910982559619 21.96115326185028 54-55 22.081454543852132 27.46394541935242 28.327041018110187 22.127559018685254 56-57 22.03700255915639 27.807387952906232 28.142678201782267 22.01293128615511 58-59 21.807292360945098 27.76234222780889 28.562206062021534 21.868159349224484 60-61 22.05395996518712 27.885117493472585 28.221458395302463 21.83946414603783 62-63 21.880364198061343 28.078980827157963 28.098592170006103 21.94206280477459 64-65 21.989319128894813 27.99324664880095 28.01797955816778 21.999454664136454 66-67 21.760807773664727 28.059833862154065 28.07233826899348 22.107020095187732 68-69 21.824027797898964 27.923271541250937 28.195830204986372 22.05687045586373 70-71 21.890879406160817 27.7613538528655 28.21749646504623 22.130270275927447 72-73 21.97124691100953 27.720818060527137 28.084500897363434 22.223434131099896 74-75 21.933464145754858 27.83412529567757 27.915984051314314 22.316426507253258 76-77 21.948044794288894 27.92399513057656 27.78837838284465 22.339581692289894 78-79 21.888577975587186 27.903483453047194 27.932512228440487 22.27542634292513 80-81 21.885595706272078 27.889455125574354 27.890887396511538 22.334061771642027 82-83 22.019379138201824 27.867385832592852 27.827887162711935 22.285347866493392 84-85 22.136389100197256 27.762669459058536 27.65156501923258 22.449376421511626 86-87 21.843116776669124 27.851278000500162 27.809799858324325 22.49580536450639 88-89 21.93025701249892 27.745055322768014 27.826137728822314 22.49854993591075 90-91 21.981368724530995 27.64390159531398 27.928515479976777 22.44621420017825 92-93 21.995359807694214 27.62137264644092 27.8285398266648 22.554727719200063 94-95 21.96115326185028 27.636245057677417 27.780507446671386 22.622094233800915 96-97 22.00334684335826 27.60490282807162 27.733025896723774 22.65872443184635 98-99 21.875554272733584 27.631452836278992 27.706475888516408 22.786517002471022 100-101 21.850930329017928 27.686232430500052 27.614514002995428 22.848323237486596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1119.0 1 683.0 2 238.0 3 216.0 4 183.0 5 183.0 6 279.0 7 524.5 8 808.0 9 900.5 10 782.0 11 648.0 12 621.5 13 679.5 14 757.5 15 834.5 16 914.5 17 998.0 18 1089.5 19 1193.0 20 1323.5 21 1463.5 22 1741.0 23 2169.5 24 2705.0 25 3447.0 26 4532.5 27 5912.0 28 7456.0 29 9295.5 30 11429.5 31 13435.0 32 15765.0 33 17995.0 34 20016.0 35 21767.0 36 23851.5 37 25988.5 38 27405.0 39 28877.0 40 30060.0 41 31180.5 42 32183.5 43 33347.0 44 34374.0 45 35183.5 46 35709.0 47 36168.0 48 36212.5 49 35403.5 50 34139.5 51 32570.5 52 31032.0 53 29293.5 54 27459.5 55 25362.0 56 22608.5 57 19753.0 58 17527.5 59 15249.5 60 12609.0 61 10167.0 62 8020.5 63 6118.0 64 4462.0 65 3197.5 66 2305.0 67 1690.0 68 1303.5 69 990.5 70 688.0 71 471.5 72 356.0 73 276.0 74 207.5 75 134.5 76 80.5 77 51.5 78 36.0 79 23.5 80 15.0 81 10.0 82 8.5 83 5.0 84 1.5 85 1.5 86 2.5 87 1.5 88 0.0 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01762656146547232 2 0.0 3 0.0015423241282288278 4 0.01696556541051711 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.5083004579601E-5 14-15 5.5083004579601E-5 16-17 0.00165249013738803 18-19 1.65249013738803E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.5083004579601E-4 28-29 0.006554877544972519 30-31 1.65249013738803E-4 32-33 1.10166009159202E-4 34-35 0.011953011993773417 36-37 0.004847304403004888 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.40664036636808E-4 60-61 0.001211826100751222 62-63 0.009143778760213765 64-65 0.002588901215241247 66-67 0.004516806375527282 68-69 0.0029193992427188533 70-71 5.5083004579601E-5 72-73 0.006830292567870523 74-75 0.00660996054955212 76-77 0.001762656146547232 78-79 1.10166009159202E-4 80-81 0.007876869654882943 82-83 0.01052085387470379 84-85 0.001817739151126833 86-87 0.0015423241282288278 88-89 2.75415022898005E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.95747041216409E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 907721.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.22310993341654 #Duplication Level Percentage of deduplicated Percentage of total 1 71.12868540621477 46.39234067668946 2 18.822264751235135 24.5529328613136 3 5.8960772275868 11.536814795724228 4 2.0621788023891376 5.380068589223519 5 0.8730051861301806 2.8470056613705763 6 0.4196836494485318 1.642384368314342 7 0.23706216094178328 1.082335196891145 8 0.14131639380676123 0.7373675750921895 9 0.08365234453034055 0.4910459457141393 >10 0.32576849551682013 3.256056245885746 >50 0.005407355313474682 0.2412369640702335 >100 0.0032091832821423373 0.3638739582601536 >500 0.0010134261624658231 0.4689710285977332 >1k 6.756174416438822E-4 1.0075661328529169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.34459927664998385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2803 0.3087953236732432 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1890 0.20821375731089178 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1325 0.14596996213594265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.30498027477606E-4 0.0 24-25 0.0 0.0 0.0 6.05913050375611E-4 0.0 26-27 0.0 0.0 0.0 7.71162064114414E-4 0.0 28-29 0.0 0.0 0.0 0.001872822155706434 0.0 30-31 0.0 0.0 0.0 0.005012553416743691 0.0 32-33 0.0 0.0 0.0 0.010355604860964988 0.0 34-35 0.0 0.0 0.0 0.01795705949294993 0.0 36-37 0.0 0.0 0.0 0.028367747358494518 0.0 38-39 0.0 0.0 0.0 0.03591411898589986 0.0 40-41 0.0 0.0 0.0 0.04197324948965596 0.0 42-43 0.0 0.0 0.0 0.05095177923613092 0.0 44-45 0.0 0.0 0.0 0.0604260560238223 0.0 46-47 0.0 0.0 0.0 0.0721587359992773 0.0 48-49 0.0 0.0 0.0 0.08835313934568001 0.0 50-51 0.0 0.0 0.0 0.10306030156843347 0.0 52-53 0.0 0.0 0.0 0.12189868913465701 0.0 54-55 0.0 0.0 0.0 0.14960544043819632 0.0 56-57 0.0 0.0 0.0 0.1871169665569046 0.0 58-59 0.0 0.0 0.0 0.21862444517643637 0.0 60-61 0.0 0.0 0.0 0.2517293309287766 0.0 62-63 0.0 0.0 0.0 0.2960711496153554 0.0 64-65 0.0 0.0 0.0 0.35484471550178964 0.0 66-67 0.0 0.0 0.0 0.43658789429791756 0.0 68-69 0.0 0.0 0.0 0.5225724644466747 0.0 70-71 0.0 0.0 0.0 0.6049215562931782 0.0 72-73 0.0 0.0 0.0 0.6950373517854054 0.0 74-75 0.0 0.0 0.0 0.7990340644316921 0.0 76-77 0.0 0.0 0.0 0.9159202001496054 0.0 78-79 0.0 0.0 0.0 1.0433271897422225 0.0 80-81 0.0 0.0 0.0 1.1656114599089369 0.0 82-83 0.0 0.0 0.0 1.3066239516327154 0.0 84-85 0.0 0.0 0.0 1.4751228626417148 0.0 86-87 0.0 0.0 0.0 1.6767266594030543 0.0 88-89 0.0 0.0 0.0 1.9017958161153041 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1485 0.0 58.54737 1 GTATCAA 2600 0.0 38.738724 1 CCATACG 50 0.0016157563 37.999428 2 GTGGTAT 680 0.0 37.02966 1 TGGTATC 725 0.0 32.758125 2 TCAACGC 3120 0.0 32.275154 4 ATCAACG 3155 0.0 31.91711 3 CAACGCA 3175 0.0 31.863907 5 TATCAAC 3240 0.0 31.519588 2 AACGCAG 3330 0.0 30.523384 6 CGTACGG 35 0.008385554 27.147684 76-77 ACGCAGA 3740 0.0 27.050241 7 CGCAGAG 3785 0.0 26.479124 8 GCAGAGT 4230 0.0 22.907455 9 GAGTACT 3025 0.0 21.98435 12-13 TACATGG 1770 0.0 21.73696 2 GTACATG 1850 0.0 21.315187 1 CAGAGTA 4115 0.0 21.240217 10-11 GTACTTT 3445 0.0 19.096754 14-15 AGAGTAC 3805 0.0 18.476429 10-11 >>END_MODULE