FastQCFastQC Report
Fri 10 Feb 2017
SRR2031697_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031697_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118861
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14011.1786877108555371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.8682410546773122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.8253337932543054No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.6595939795222991No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6310.530872195253279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.4215007445671835No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4010.3373688594240331No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2590.21790158252075953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.17499432109775284No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2030.17078772684059532No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2000.1682637702863008No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1850.15564398751482825No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1840.15480266866339673No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1710.1438655235947872No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1690.14218288589192418No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1640.13797629163476666No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1580.13292837852617762No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1500.1261978277147256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12114991460613657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.11189540724039003No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1310.11021276953752702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATC608.2318285E-539.594754
GTATGTA500.001607698538.010961
CGACAGC250.001612616638.0029594-95
TAGATCA651.318775E-436.5495
AGTTCTG702.0388312E-433.9383545
GTTATAG600.003931250431.67581
GATCATG1257.325667E-626.596467
ATCAATA1109.3881376E-525.9165632
AGATCAT1109.415112E-525.9056426
ATCATGT1309.900412E-625.5843018
CCTAGAT950.001218028725.0072123
TCCTAGA1000.001642187223.7568512
ATGGGGT1401.7581606E-523.756855
CAGATTT500.001649048123.75184494-95
GAGTACT7500.023.12333512-13
TGGGGTT1050.002186406422.6160376
CATCAAT1302.914345E-421.9294011
GGTATCA15500.021.7643411
GTACTTT7900.021.05037314-15
GTACATG7850.020.5791821
Produced by FastQC (version 0.11.5)