##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031697_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 118861 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59132095472863 34.0 31.0 34.0 31.0 34.0 2 32.76742581671027 34.0 31.0 34.0 31.0 34.0 3 32.78420171460782 34.0 31.0 34.0 31.0 34.0 4 36.23906075163426 37.0 35.0 37.0 35.0 37.0 5 36.21636196902264 37.0 37.0 37.0 35.0 37.0 6 36.277475370390626 37.0 37.0 37.0 35.0 37.0 7 36.23238909314241 37.0 37.0 37.0 35.0 37.0 8 36.24110515644324 37.0 37.0 37.0 35.0 37.0 9 38.04506103768267 39.0 39.0 39.0 35.0 39.0 10-11 38.01132415174027 39.0 38.5 39.0 35.0 39.0 12-13 37.95468236006764 39.0 38.0 39.0 35.0 39.0 14-15 39.27853122554917 41.0 39.0 41.0 36.0 41.0 16-17 39.215432311691806 41.0 39.0 41.0 36.0 41.0 18-19 39.277416478071025 41.0 39.0 41.0 36.0 41.0 20-21 39.31763993235796 41.0 39.0 41.0 36.0 41.0 22-23 39.19539209665071 41.0 39.0 41.0 35.0 41.0 24-25 39.0903029589184 41.0 39.0 41.0 35.0 41.0 26-27 38.8763135090568 40.0 38.0 41.0 35.0 41.0 28-29 38.71420398616871 40.0 38.0 41.0 35.0 41.0 30-31 38.461421324067615 40.0 38.0 41.0 35.0 41.0 32-33 38.06904283154273 40.0 37.0 41.0 34.0 41.0 34-35 37.81803114562388 40.0 36.0 41.0 34.0 41.0 36-37 37.653309327702104 40.0 36.0 41.0 33.0 41.0 38-39 37.51463474142065 40.0 36.0 41.0 33.0 41.0 40-41 37.461825157116294 40.0 36.5 41.0 33.0 41.0 42-43 37.271022454800146 40.0 36.0 41.0 33.0 41.0 44-45 36.89003541952364 40.0 36.0 41.0 31.5 41.0 46-47 36.37180824660739 40.0 36.0 41.0 30.5 41.0 48-49 35.88838643457484 40.0 35.5 41.0 28.5 41.0 50-51 35.431403067448535 40.0 35.0 41.0 26.0 41.0 52-53 35.533269953979854 40.0 36.0 41.0 26.0 41.0 54-55 35.46096701188783 40.0 35.5 41.0 26.0 41.0 56-57 35.06146254869133 39.5 35.0 41.0 23.5 41.0 58-59 34.72186840090526 39.0 35.0 41.0 20.0 41.0 60-61 34.279212693818835 39.0 35.0 41.0 10.0 41.0 62-63 33.83565256896711 39.0 35.0 41.0 2.0 41.0 64-65 33.285114545561626 38.0 34.0 40.5 2.0 41.0 66-67 32.5903912973978 37.5 34.0 40.0 2.0 41.0 68-69 31.817118314670076 37.0 33.0 40.0 2.0 41.0 70-71 31.355890493938297 36.5 33.0 39.0 2.0 41.0 72-73 30.96919090366058 36.0 32.5 39.0 2.0 41.0 74-75 30.58552847443653 35.5 32.0 38.5 2.0 40.0 76-77 29.728178292290995 35.0 30.5 37.0 2.0 39.0 78-79 29.88234997181582 35.0 31.5 37.0 2.0 39.0 80-81 29.63822027410168 35.0 32.0 36.5 2.0 39.0 82-83 29.316899571768705 35.0 31.5 36.0 2.0 37.5 84-85 28.999158681148568 35.0 31.0 36.0 2.0 37.0 86-87 28.769659518260823 35.0 31.0 35.0 2.0 37.0 88-89 28.528100049637814 35.0 31.0 35.0 2.0 36.0 90-91 28.299968029883644 35.0 30.5 35.0 2.0 36.0 92-93 28.109661705689838 35.0 30.0 35.0 2.0 36.0 94-95 27.899853610519852 35.0 30.0 35.0 2.0 36.0 96-97 27.693179428071446 35.0 29.0 35.0 2.0 35.0 98-99 27.50356719193007 35.0 29.0 35.0 2.0 35.0 100-101 26.872456062122986 34.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11753842293623507 1101 2 0.09813235934449693 1101 3 0.2315006208537369 1101 4 -0.09276818579916579 1101 5 -0.0414694243307423 1101 6 -0.05976548657205427 1101 7 0.05175203203715739 1101 8 0.20387380737699345 1101 9 -0.022202838580462014 1101 10-11 0.16885685126194971 1101 12-13 0.16880299612402183 1101 14-15 -0.019123357128982832 1101 16-17 0.06270131146674629 1101 18-19 -0.2600813155204733 1101 20-21 -0.20373300790897986 1101 22-23 -0.11628019412231083 1101 24-25 -0.04912873852818933 1101 26-27 0.37947235262807055 1101 28-29 0.003615043515274863 1101 30-31 -0.03672434444037975 1101 32-33 0.21234260349768164 1101 34-35 0.11931164166310992 1101 36-37 0.47979828269691893 1101 38-39 0.4717932505192408 1101 40-41 -0.3229991009635711 1101 42-43 0.1904543903575231 1101 44-45 0.35107139649005603 1101 46-47 0.752098846643797 1101 48-49 0.5675752626039383 1101 50-51 1.457245716153686 1101 52-53 1.3145742572161936 1101 54-55 1.1015672075686567 1101 56-57 1.114599343057769 1101 58-59 1.1769295543460956 1101 60-61 0.8571718881816963 1101 62-63 1.3162532922715187 1101 64-65 1.653845656511315 1101 66-67 1.6573772972817338 1101 68-69 1.5577182907943943 1101 70-71 1.2058680863372615 1101 72-73 1.3751267250641455 1101 74-75 1.4235166443350913 1101 76-77 1.299110180938726 1101 78-79 1.031681970570304 1101 80-81 1.174048291282606 1101 82-83 1.253102968286477 1101 84-85 1.007084788335682 1101 86-87 0.949435699073927 1101 88-89 1.552701174522408 1101 90-91 1.1744241035825098 1101 92-93 0.8262681741790274 1101 94-95 0.8155736282113182 1101 96-97 1.3634949084454355 1101 98-99 1.3536691160413703 1101 100-101 0.9432770883998742 1102 1 -0.035973359264630744 1102 2 0.07525429940397999 1102 3 -0.17675835878478807 1102 4 -0.026650592571066056 1102 5 0.035744027968505065 1102 6 0.061145060213583236 1102 7 0.08744180554435843 1102 8 -0.01689947104899403 1102 9 -0.07527993764246332 1102 10-11 0.02604056208327421 1102 12-13 -0.08228371172315008 1102 14-15 -0.27930409380251575 1102 16-17 -0.13811085966113978 1102 18-19 -0.49092094032030076 1102 20-21 -0.15923518132466796 1102 22-23 -0.14190362125568612 1102 24-25 -0.04312324779380816 1102 26-27 -0.0798618958276549 1102 28-29 -0.3321204861118119 1102 30-31 -1.0913457125483674 1102 32-33 -0.3971861084675439 1102 34-35 -0.5047446383643361 1102 36-37 -1.0457107541367492 1102 38-39 -1.9581998860627792 1102 40-41 -1.660050119037244 1102 42-43 -2.175194202641798 1102 44-45 -1.7909441605907048 1102 46-47 -2.0365078795058196 1102 48-49 -1.7225757325916717 1102 50-51 -1.9523287519314252 1102 52-53 -1.7406190623903015 1102 54-55 -1.402665233520338 1102 56-57 -1.03330731445768 1102 58-59 -1.0816497181315938 1102 60-61 -1.668337148651979 1102 62-63 -1.262504429073715 1102 64-65 -1.1262389923104692 1102 66-67 -1.1947160452028172 1102 68-69 -0.9329588997798446 1102 70-71 -1.083768152509684 1102 72-73 -1.432869156885065 1102 74-75 -0.9851998965022482 1102 76-77 -1.533937176645349 1102 78-79 -1.436803501861636 1102 80-81 -1.2523987460086339 1102 82-83 -1.1703699212124903 1102 84-85 -1.0407303569399957 1102 86-87 -1.230557437508093 1102 88-89 -1.0161045907487 1102 90-91 -0.9550085205309635 1102 92-93 -1.3517231321581953 1102 94-95 -1.456274919031877 1102 96-97 -1.6037322706897754 1102 98-99 -1.3199753589071541 1102 100-101 -0.9837990955397267 1103 1 0.3749033983863228 1103 2 0.14886885688950002 1103 3 0.3630472656982562 1103 4 0.32560333629576377 1103 5 0.12383335799331974 1103 6 0.0032459700564260174 1103 7 0.040295403559248655 1103 8 0.05309639330170768 1103 9 -0.08603262581450366 1103 10-11 0.12384866795589033 1103 12-13 0.269866825911258 1103 14-15 0.5959543842785351 1103 16-17 0.8632332263603644 1103 18-19 0.573388406247112 1103 20-21 0.4560460428275164 1103 22-23 0.44458314466655935 1103 24-25 0.5750529308662422 1103 26-27 0.41967284362643653 1103 28-29 0.6549039142190409 1103 30-31 0.7017167357560226 1103 32-33 0.610051277802107 1103 34-35 0.5219302996009176 1103 36-37 0.39060024669036864 1103 38-39 0.7640395680315137 1103 40-41 0.22947634911826498 1103 42-43 0.05635046237060948 1103 44-45 -0.27802025653776496 1103 46-47 -0.2920910060566939 1103 48-49 0.36892550810311775 1103 50-51 -0.09655952116219879 1103 52-53 -0.8553316347641626 1103 54-55 -0.5721234634624395 1103 56-57 -0.7262353541599111 1103 58-59 -1.030005797536063 1103 60-61 -0.74644922474792 1103 62-63 -0.791970930314406 1103 64-65 -0.6155896953708506 1103 66-67 -0.5690956978082795 1103 68-69 -0.6121876011859619 1103 70-71 -0.44450016243524004 1103 72-73 -0.4427947151977172 1103 74-75 -0.22449890394641514 1103 76-77 -0.24190864066520135 1103 78-79 -0.30782293941333094 1103 80-81 -0.41156954832459647 1103 82-83 -0.3302644621554194 1103 84-85 -0.6566836241029428 1103 86-87 -0.4554333680291833 1103 88-89 -0.5531703475725216 1103 90-91 -0.5046880077104525 1103 92-93 0.05253658661765215 1103 94-95 0.006142367982185704 1103 96-97 -0.44880050890317236 1103 98-99 -0.6512408675920192 1103 100-101 -0.9876762667556065 1104 1 -0.21370896963781405 1104 2 -0.24479630148153575 1104 3 -0.06512015511622593 1104 4 -0.19268474816879433 1104 5 -0.04584989992522992 1104 6 0.03869091726608076 1104 7 -0.07131847276050962 1104 8 0.10833772060185964 1104 9 0.29632031536196735 1104 10-11 0.1713599801730865 1104 12-13 0.005160943558315978 1104 14-15 0.36886162409754064 1104 16-17 0.14509597145840303 1104 18-19 0.0854547712546534 1104 20-21 0.04032206092706758 1104 22-23 -0.5372230393455055 1104 24-25 -0.5341099650613614 1104 26-27 -0.368128815498757 1104 28-29 -0.14552972077341764 1104 30-31 -0.7902704437311527 1104 32-33 -0.5328597327559734 1104 34-35 -0.8728660804895796 1104 36-37 -0.8684495270652803 1104 38-39 -0.9947274002942734 1104 40-41 -1.1096073612889725 1104 42-43 -0.5836382254121943 1104 44-45 -0.12775630480322775 1104 46-47 -0.5300246410491525 1104 48-49 -0.8993625763614403 1104 50-51 -1.1140934578137731 1104 52-53 -1.062250940948175 1104 54-55 -0.7450118495861204 1104 56-57 -0.6349834687903808 1104 58-59 -0.6085314385616982 1104 60-61 -0.39303670589423234 1104 62-63 -1.2759452892887708 1104 64-65 -0.6120074932592345 1104 66-67 -1.3381379300858072 1104 68-69 -1.7945971034484067 1104 70-71 -1.7010989557987415 1104 72-73 -1.8047856654239638 1104 74-75 -2.0805509401455105 1104 76-77 -1.6980737990362407 1104 78-79 -1.7226985050241872 1104 80-81 -1.7497105739656256 1104 82-83 -1.981565367132795 1104 84-85 -2.4284596422024904 1104 86-87 -2.1494378929160582 1104 88-89 -1.9258136356569864 1104 90-91 -2.2491261675897896 1104 92-93 -1.8166187678318195 1104 94-95 -1.8044722624853868 1104 96-97 -1.7579068437448022 1104 98-99 -1.2719045176674335 1104 100-101 -0.7896181973740113 1105 1 0.0486796221625454 1105 2 0.19851920653985644 1105 3 0.03594936360035206 1105 4 -0.050795265102841825 1105 5 -0.04050230634234708 1105 6 -0.17717361036315538 1105 7 0.11442127768512478 1105 8 0.15397051917583582 1105 9 -0.20421444399632094 1105 10-11 0.028044472151698585 1105 12-13 -0.09831499227055929 1105 14-15 -0.02231484649069415 1105 16-17 -0.03903950091236652 1105 18-19 -0.11017802731931425 1105 20-21 -0.26021269843122496 1105 22-23 -0.037846925263508524 1105 24-25 0.051451532264842115 1105 26-27 0.01573927719287127 1105 28-29 0.18156475337988098 1105 30-31 -0.311482564943276 1105 32-33 0.11214917990000828 1105 34-35 -0.7207270430564208 1105 36-37 -0.33422492813480176 1105 38-39 -0.3570268655349196 1105 40-41 -0.5045271473852324 1105 42-43 -1.1769537334335851 1105 44-45 -1.8823908859083929 1105 46-47 -1.5574756214413128 1105 48-49 -1.3892912751136635 1105 50-51 -0.5432378428405187 1105 52-53 -1.366083383015912 1105 54-55 -1.6184521347911058 1105 56-57 -1.107354235278791 1105 58-59 -0.7433799234101883 1105 60-61 -1.1613967771954776 1105 62-63 -1.1547331680766462 1105 64-65 -0.8028274576086076 1105 66-67 -1.6524873061998875 1105 68-69 -0.7683938948922595 1105 70-71 -0.20341624635132405 1105 72-73 -0.21761989002289184 1105 74-75 -0.13402687597438856 1105 76-77 -0.7002128364693974 1105 78-79 -0.38098727507766483 1105 80-81 -0.35274087699592727 1105 82-83 -0.14573649012744738 1105 84-85 0.5448023898830705 1105 86-87 0.6797064921106788 1105 88-89 0.12436461746244376 1105 90-91 -0.1565236720461165 1105 92-93 -0.6296312455501791 1105 94-95 -0.8797842054443876 1105 96-97 -1.0116501592528238 1105 98-99 -1.3473947137458673 1105 100-101 -0.775001441580784 1106 1 -0.09677492329200277 1106 2 -0.08329897527833197 1106 3 -0.21859609094510546 1106 4 -0.07806799237556561 1106 5 -0.031411397251439155 1106 6 -0.35899179218133526 1106 7 -0.12194235867850978 1106 8 -0.07330220809689791 1106 9 -0.14057808035995834 1106 10-11 -0.1265009823937575 1106 12-13 -0.30740590136147006 1106 14-15 -0.39504211921796895 1106 16-17 -0.08903950091236368 1106 18-19 0.14891288177158657 1106 20-21 0.09433275611423397 1106 22-23 0.17578943837285266 1106 24-25 -0.0485484677351522 1106 26-27 0.3066483681019605 1106 28-29 -0.20479888298375215 1106 30-31 0.13397198051126935 1106 32-33 0.2485128162636414 1106 34-35 -0.17527249760187402 1106 36-37 0.10213870822883564 1106 38-39 0.46570040719235095 1106 40-41 0.586381943523854 1106 42-43 0.486682630202786 1106 44-45 0.6312454777279655 1106 46-47 -0.10747562144130995 1106 48-49 -0.32565491147730086 1106 50-51 -0.4205105701132439 1106 52-53 -0.10244701937954659 1106 54-55 -0.20481577115474892 1106 56-57 -0.28917241709697095 1106 58-59 0.49298371295344623 1106 60-61 0.03860322280452522 1106 62-63 0.09526683192335383 1106 64-65 0.5153543605732054 1106 66-67 0.8475126938001125 1106 68-69 1.0406970141986456 1106 70-71 0.6647655718304932 1106 72-73 0.4914710190680154 1106 74-75 0.33415494220743014 1106 76-77 0.57705989080333 1106 78-79 0.5962854521950618 1106 80-81 0.8154409411858907 1106 82-83 0.8815362371452764 1106 84-85 0.640256935337618 1106 86-87 0.1478883102924975 1106 88-89 1.069819162916989 1106 90-91 1.0162036006811555 1106 92-93 1.3612778453589094 1106 94-95 1.1474885218283362 1106 96-97 1.0474407498380884 1106 98-99 0.9707871044359528 1106 100-101 1.3931803766010376 1107 1 0.3274675009504193 1107 2 0.11150621952686635 1107 3 0.05672858437957018 1107 4 0.005914691607117106 1107 5 -0.0413681072081431 1107 6 0.16222032903078798 1107 7 0.04688881015265878 1107 8 -0.17849701329169676 1107 9 -0.10508024486212264 1107 10-11 0.17458126868849178 1107 12-13 0.029390635435071033 1107 14-15 -0.16517198934783295 1107 16-17 0.00966179778893661 1107 18-19 -0.0062819234232094345 1107 20-21 0.15796911975059658 1107 22-23 0.19526995785337675 1107 24-25 0.05426538507870049 1107 26-27 -0.197680636227048 1107 28-29 -0.24917117735605387 1107 30-31 -0.7093180627787774 1107 32-33 -0.5776776599268345 1107 34-35 -0.924406696736078 1107 36-37 -1.1526231965330709 1107 38-39 -1.6102736187816689 1107 40-41 -0.9963020391601276 1107 42-43 -1.1137502702301205 1107 44-45 -0.7176722711897838 1107 46-47 -0.8312851452508312 1107 48-49 -0.8864774222998122 1107 50-51 -1.4523287519314252 1107 52-53 -0.8905422574747845 1107 54-55 -1.176244342583317 1107 56-57 -1.7110338889584469 1107 58-59 -1.5942457242759858 1107 60-61 -1.8419162577149564 1107 62-63 -1.1021357654792467 1107 64-65 -1.1935200983012564 1107 66-67 -1.1407989945115773 1107 68-69 -2.195017271515635 1107 70-71 -2.415104558039637 1107 72-73 -2.966970539373545 1107 74-75 -2.399178391125904 1107 76-77 -1.7417712780278372 1107 78-79 -1.8610738551642463 1107 80-81 -2.2616153358703848 1107 82-83 -2.459156403547361 1107 84-85 -2.3365828915022107 1107 86-87 -2.0683454559412695 1107 88-89 -2.1674102743124486 1107 90-91 -1.8169132824357277 1107 92-93 -1.5443498602687953 1107 94-95 -1.7167971924573777 1107 96-97 -1.3129488605515256 1107 98-99 -2.173585189936343 1107 100-101 -2.505304471883811 1108 1 0.1336299659364144 1108 2 -0.05151899055717024 1108 3 -0.13654872654479533 1108 4 -0.166074103911086 1108 5 0.0802005202428333 1108 6 -0.027135413266130115 1108 7 -0.0657162318641511 1108 8 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-1.3895537086440974 2316 80-81 -0.8683003175553665 2316 82-83 -0.8895593339502916 2316 84-85 -0.603799008718326 2316 86-87 -0.4362025987984133 2316 88-89 -0.3688754757776458 2316 90-91 -0.4453931359155838 2316 92-93 -0.27759161851055225 2316 94-95 -0.0852037858639676 2316 96-97 -0.07540307300573801 2316 98-99 -0.3243177906689425 2316 100-101 0.02354168196234241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 5.0 7 16.0 8 47.0 9 70.0 10 123.0 11 254.0 12 360.0 13 530.0 14 666.0 15 990.0 16 1333.0 17 2010.0 18 2049.0 19 1133.0 20 892.0 21 1070.0 22 1391.0 23 1536.0 24 1747.0 25 2193.0 26 1515.0 27 810.0 28 1013.0 29 1215.0 30 1485.0 31 1758.0 32 2268.0 33 2799.0 34 4017.0 35 7267.0 36 9864.0 37 20650.0 38 35071.0 39 10695.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.355767046171714 22.576763520393136 16.41632797314013 21.65114146029502 2 14.887137076080464 23.36594846080716 41.078234239994615 20.66868022311776 3 17.314341962460354 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22-23 19.60609451375977 28.89257199586071 32.50477448448187 18.996559005897645 24-25 20.12392626681586 29.593811258528866 32.76852794440565 17.513734530249618 26-27 19.54938772763029 29.520320039037358 33.94273118487639 16.987561048455962 28-29 17.717833944737993 28.481220341264766 33.98554504762225 19.815400666374988 30-31 19.934713382494603 28.388320762574615 33.57002536587007 18.106940489060705 32-33 20.00992756244689 28.93169332245228 33.982130387595596 17.076248727505234 34-35 19.329000231379233 28.979617593235314 34.357291600933934 17.334090574451526 36-37 19.451537319271914 28.003651369894705 33.82270664103416 18.722104669799215 38-39 17.993706934991295 27.693692632570816 35.19447085250839 19.118129579929498 40-41 17.614692792421398 27.44718620910139 35.73039096087026 19.20773003760695 42-43 19.053768687794985 27.312995852298062 34.54371072092612 19.089524738980828 44-45 18.136310480308932 28.240549886001297 34.282481217556644 19.34065841613313 46-47 20.247179478550574 27.477473687752923 32.679348146153906 19.595998687542593 48-49 19.428996895533437 27.492617427078684 33.174884949647065 19.903500727740806 50-51 18.317194033366704 28.214048342181204 33.38731795963352 20.08143966481857 52-53 18.606187058833427 29.611899613834648 33.53328678035689 18.24862654697504 54-55 18.482513187672996 29.336788349416544 31.882198534422564 20.2984999284879 56-57 20.013292837852617 30.182734454530923 30.13562059885076 19.668352108765703 58-59 18.624774420434036 30.901350743098 32.334543435371714 18.139331401096243 60-61 19.542903776743874 32.83554463692274 30.059145710463657 17.56240587586973 62-63 17.709222931329695 34.10250693097631 29.847160929066348 18.341109208627646 64-65 17.927468986492457 34.131474556093536 29.48817721763931 18.452879239774692 66-67 18.3049592582777 33.26897108746956 29.16072472730179 19.265344926950952 68-69 17.924179069317425 32.92178127024458 29.863535786099497 19.2905038743385 70-71 18.822406003651324 32.35544038835278 29.865136588115533 18.957017019880364 72-73 18.74224366767771 31.599981489973032 30.356444223819878 19.301330618529377 74-75 18.437138469952252 32.217664752529394 29.5740518710166 19.771144906501757 76-77 18.239335007593063 31.61700677696589 30.001640606267117 20.142017609173934 78-79 18.4799892311187 31.6054046323016 29.819705370138227 20.094900766441473 80-81 18.924058088074442 31.349807326636043 29.258586164538002 20.46754842075151 82-83 18.924956458086175 30.82935776728845 29.929491548241078 20.316194226384297 84-85 18.754416961130744 30.650344943631165 29.63528520949016 20.95995288574794 86-87 18.432952915392413 31.033017697216486 29.779867996516924 20.754161390874177 88-89 18.97216075920613 30.613489706463852 29.203439311464653 21.210910222865365 90-91 18.97216075920613 30.084300148913435 29.608113679003207 21.335425412877225 92-93 19.40501930826764 30.62484751095818 29.57698488150024 20.393148299273943 94-95 19.2169845449727 29.78184602182381 29.699396774383523 21.301772658819967 96-97 18.874567772440077 29.905099233558524 29.824753283246817 21.39557971075458 98-99 18.959540976434656 30.306408325691354 29.05999444729558 21.674056250578406 100-101 18.821564684799892 29.927394183121457 29.177358427070278 22.07368270500837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1539.0 1 883.0 2 229.0 3 205.0 4 129.5 5 101.5 6 168.5 7 330.5 8 495.0 9 483.0 10 344.5 11 229.5 12 204.5 13 214.5 14 226.5 15 257.0 16 276.5 17 281.0 18 280.5 19 295.5 20 348.5 21 381.5 22 390.5 23 446.5 24 538.5 25 631.0 26 820.5 27 1085.5 28 1269.0 29 1517.5 30 1951.5 31 2421.0 32 3032.5 33 3791.0 34 4432.5 35 4997.0 36 5723.5 37 6182.5 38 5759.5 39 5245.5 40 4948.0 41 4641.5 42 4366.0 43 4256.5 44 4238.5 45 4142.0 46 3899.0 47 4005.0 48 4796.5 49 4462.0 50 3194.0 51 2437.5 52 1928.0 53 1643.5 54 1494.5 55 1288.5 56 1116.5 57 946.0 58 811.5 59 729.0 60 598.5 61 454.0 62 328.5 63 230.5 64 171.5 65 102.0 66 64.0 67 50.0 68 39.5 69 28.0 70 15.5 71 6.5 72 9.5 73 16.5 74 13.0 75 6.0 76 4.5 77 3.5 78 1.5 79 1.5 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01850901473149309 2 0.0 3 0.0 4 0.023556927840082113 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.20659425715752E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.20659425715752E-4 28-29 0.0075718696628835356 30-31 4.20659425715752E-4 32-33 0.0 34-35 0.007151210237167784 36-37 0.001261978277147256 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.20659425715752E-4 60-61 0.001682637702863008 62-63 0.008833847940030792 64-65 0.001261978277147256 66-67 0.00210329712857876 68-69 0.001682637702863008 70-71 0.0 72-73 0.005468572534304776 74-75 0.007151210237167784 76-77 0.00210329712857876 78-79 0.0 80-81 0.005889231960020528 82-83 0.00841318851431504 84-85 8.41318851431504E-4 86-87 4.20659425715752E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 118861.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.60939248365738 #Duplication Level Percentage of deduplicated Percentage of total 1 72.27022919648695 34.40741706699422 2 15.075368004382478 14.354582243124323 3 5.446288147873261 7.778834100335687 4 2.3131704041421477 4.405145506095355 5 1.180441428546184 2.8100049637812234 6 0.7386594567848875 2.110027679390212 7 0.47712452950220013 1.5900926292055428 8 0.3887681351499408 1.4807211785194472 9 0.3039459965717719 1.3023615820159682 >10 1.6752372369188357 14.318405532512768 >50 0.07245224336885261 2.274084855419355 >100 0.040643941402039266 3.8078091215789875 >500 0.008835639435225928 3.2054248239540306 >1k 0.008835639435225928 6.155088717072883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2662 2.239590782510664 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1324 1.1139061592953114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 1.0104239405692363 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1067 0.8976872144774148 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1062 0.8934806202202572 RNA PCR Primer, Index 23 (95% over 23bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.8186032424428533 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 783 0.6587526606708676 RNA PCR Primer, Index 23 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 745 0.6267825443164705 RNA PCR Primer, Index 23 (96% over 25bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.5510638476876352 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 654 0.5502225288362036 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 413 0.34746468564121114 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 352 0.2961442357038894 RNA PCR Primer, Index 23 (96% over 28bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.25155433657801973 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 278 0.23388664069795814 No Hit CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 240 0.20191652434356097 Illumina PCR Primer Index 1 (95% over 23bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 231 0.19434465468067744 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 224 0.1884554227206569 No Hit CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA 213 0.17920091535491037 RNA PCR Primer, Index 23 (96% over 30bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 213 0.17920091535491037 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.1657398137320063 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.16069190062341726 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 186 0.15648530636625974 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 170 0.14302420474335567 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 166 0.13965892933762966 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 165 0.13881761048619817 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 137 0.11526068264611607 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.11441936379468455 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 125 0.10516485642893801 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC 123 0.103482218726075 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.10179958102321199 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 120 0.10095826217178049 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 119 0.10011694332034898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.20659425715752E-4 0.0 22-23 0.0 0.0 0.0 0.003365275405726016 0.0 24-25 0.0 0.0 0.0 0.005889231960020528 0.0 26-27 0.0 0.0 0.0 0.009254507365746545 0.0 28-29 0.0 0.0 0.0 0.017247036454345832 0.0 30-31 0.0 0.0 0.0 0.026080884394376627 0.0 32-33 0.0 0.0 0.0 0.04332792084872246 0.0 34-35 0.0 0.0 0.0 0.07571869662883537 0.0 36-37 0.0 0.0 0.0 0.12157057403185233 0.0 38-39 0.0 0.0 0.0 0.15354069038624948 0.0 40-41 0.0 0.0 0.0 0.17078772684059532 0.0 42-43 0.0 0.0 0.0 0.19350333582924592 0.0 44-45 0.0 0.0 0.0 0.2208461985007698 0.0 46-47 0.0 0.0 0.0 0.2624914816466293 0.0 48-49 0.0 0.0 0.0 0.31549456928681396 0.0 50-51 0.0 0.0 0.0 0.3533539176012317 0.0 52-53 0.0 0.0 0.0 0.4034123892614062 0.0 54-55 0.0 0.0 0.0 0.4690352596730635 0.0 56-57 0.0 0.0 0.0 0.5657869275876865 0.0 58-59 0.0 0.0 0.0 0.6490774938794053 0.0 60-61 0.0 0.0 0.0 0.7247961905082407 0.0 62-63 0.0 0.0 0.0 0.8181825830171376 0.0 64-65 0.0 0.0 0.0 0.936387881643264 0.0 66-67 0.0 0.0 0.0 1.0937145068609553 0.0 68-69 0.0 0.0 0.0 1.2922657557987902 0.0 70-71 0.0 0.0 0.0 1.4697840334508374 0.0 72-73 0.0 0.0 0.0 1.6489849488057478 0.0 74-75 0.0 0.0 0.0 1.8517427920007403 0.0 76-77 0.0 0.0 0.0 2.0738509687786575 0.0 78-79 0.0 0.0 0.0 2.333818493870992 0.0 80-81 0.0 0.0 0.0 2.5891587652804535 0.0 82-83 0.0 0.0 0.0 2.8407131018584733 0.0 84-85 0.0 0.0 0.0 3.1288648084737636 0.0 86-87 0.0 0.0 0.0 3.436787508097694 0.0 88-89 0.0 0.0 0.0 3.7968719765103778 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 25 5.2558906E-5 76.0 1 CGTCAGA 25 5.2558906E-5 76.0 2 TATACAC 820 0.0 35.914635 3 CTTATAC 785 0.0 35.700638 1 GGTTACT 55 0.0025703688 34.545456 8 GAGCGTT 35 2.0484536E-4 33.92857 82-83 TTATACA 855 0.0 33.88889 2 ATTGTAC 85 1.6199725E-5 33.52941 6 TACACAT 960 0.0 32.161457 5 CGTTCTA 45 2.4049774E-5 31.666668 84-85 GTATAGG 60 0.0039368374 31.666668 2 GGTTCCA 30 0.0039447043 31.666668 86-87 ATACACA 1060 0.0 30.4717 4 CCTATTG 95 3.4648496E-5 30.000002 3 CTGAGCG 40 4.483042E-4 29.687498 80-81 GCGTTCT 40 4.483042E-4 29.687498 84-85 TTTAGGG 65 0.005823289 29.23077 3 TAGGGGG 65 0.005823289 29.23077 5 ACACATC 1010 0.0 29.158415 6 CACATCT 995 0.0 29.120604 7 >>END_MODULE