FastQCFastQC Report
Fri 10 Feb 2017
SRR2031696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268288
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.691421159351145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.6865756202290076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.3048962309160306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.29259601622137404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.19046696087786258No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.13082955629770993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.062.395831
GCGTCAG751.3586032E-744.328981
TGGTATC3700.041.0770452
CGTCAGA853.629575E-739.1138042
GTATCAA18100.037.7863431
GTGGTAT4750.032.9967581
TATCAAC21500.031.1481022
ATCAACG21850.030.6491623
TCAACGC22100.030.302454
CAACGCA22500.029.5526525
TATAGAC650.00583898129.2278962
AACGCAG23250.028.599346
CTACACG700.00838430427.140194
GAGTACT14950.025.73806212-13
ACGCAGA26450.024.959747
CGCAGAG26700.024.7260348
CATGGGG8550.023.8865414
ACATGGG19300.023.8707123
GTACATG21150.023.8038061
TACATGG20800.023.5193232
Produced by FastQC (version 0.11.5)