##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031696_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268288 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439412124284353 33.0 31.0 34.0 30.0 34.0 2 31.730901121183205 33.0 31.0 34.0 30.0 34.0 3 31.79607734971374 34.0 31.0 34.0 30.0 34.0 4 35.18827901359733 37.0 35.0 37.0 33.0 37.0 5 35.18558042104008 37.0 35.0 37.0 33.0 37.0 6 35.23276479007634 37.0 37.0 37.0 33.0 37.0 7 35.21447847089695 37.0 36.0 37.0 33.0 37.0 8 35.218269173425576 37.0 37.0 37.0 33.0 37.0 9 36.93002668773855 39.0 38.0 39.0 34.0 39.0 10-11 36.85936381798665 39.0 38.0 39.0 34.0 39.0 12-13 36.8039271230916 39.0 38.0 39.0 34.0 39.0 14-15 38.06983540076335 41.0 38.0 41.0 34.0 41.0 16-17 37.99095934219943 41.0 38.0 41.0 33.5 41.0 18-19 38.061051929270036 41.0 38.5 41.0 34.0 41.0 20-21 38.10513888060592 41.0 39.0 41.0 34.0 41.0 22-23 37.99897498210878 41.0 39.0 41.0 34.0 41.0 24-25 37.90756947757634 41.0 38.0 41.0 34.0 41.0 26-27 37.78096299499046 40.0 38.0 41.0 33.5 41.0 28-29 37.66546211533874 40.0 38.0 41.0 33.0 41.0 30-31 37.43234136450381 40.0 38.0 41.0 33.0 41.0 32-33 37.169006813573475 40.0 37.0 41.0 32.5 41.0 34-35 36.96602717974714 40.0 37.0 41.0 32.0 41.0 36-37 36.7695293863311 40.0 37.0 41.0 31.0 41.0 38-39 36.54220278208493 40.0 36.0 41.0 30.5 41.0 40-41 36.405646171278626 40.0 36.0 41.0 30.0 41.0 42-43 36.28008520694179 40.0 36.0 41.0 30.0 41.0 44-45 36.02143964694656 40.0 36.0 41.0 29.0 41.0 46-47 35.71850772304389 40.0 36.5 41.0 26.0 41.0 48-49 35.544336682967554 40.0 37.0 41.0 23.5 41.0 50-51 34.94493790255248 39.5 36.0 40.5 21.5 41.0 52-53 34.93970844763836 39.5 36.0 40.5 20.0 41.0 54-55 35.14223520992366 40.0 36.0 41.0 19.5 41.0 56-57 35.02035126431298 40.0 36.0 41.0 16.5 41.0 58-59 34.848028983778626 40.0 35.0 41.0 13.5 41.0 60-61 34.6316998896708 39.5 35.0 41.0 11.0 41.0 62-63 34.36038324487119 39.0 35.0 41.0 8.0 41.0 64-65 34.10706405057252 39.0 35.0 41.0 6.5 41.0 66-67 33.81491904222328 38.5 35.0 41.0 2.0 41.0 68-69 33.47853053435115 37.5 35.0 40.0 2.0 41.0 70-71 33.10988378160782 37.0 34.0 39.5 2.0 41.0 72-73 32.72459446564885 36.5 34.0 39.0 2.0 41.0 74-75 32.30120243916985 36.0 34.0 39.0 2.0 41.0 76-77 31.910545756798662 35.5 34.0 38.0 2.0 40.0 78-79 31.518269546159353 35.0 33.5 37.0 2.0 39.0 80-81 31.159349281369273 35.0 33.0 37.0 2.0 39.0 82-83 30.830009169250957 35.0 33.0 36.0 2.0 38.5 84-85 30.479590961951338 35.0 33.0 36.0 2.0 37.0 86-87 30.198935472328245 35.0 32.0 36.0 2.0 37.0 88-89 29.9082851264313 35.0 32.0 35.0 2.0 36.5 90-91 29.68349870288645 35.0 32.0 35.0 2.0 36.0 92-93 29.47825471135496 35.0 32.0 35.0 2.0 36.0 94-95 29.242381321564885 35.0 31.5 35.0 2.0 36.0 96-97 28.984566957895993 35.0 31.0 35.0 2.0 35.0 98-99 28.76588032263836 35.0 31.0 35.0 2.0 35.0 100-101 28.233271708015266 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20447491747440338 1101 2 -0.2746888071746163 1101 3 -0.002265973828340151 1101 4 0.09543707468920104 1101 5 -0.10558997853960506 1101 6 0.016142235266038085 1101 7 -0.14315177374719212 1101 8 0.029519771525158944 1101 9 -0.08708789224441205 1101 10-11 0.11150656026283201 1101 12-13 -0.06269541380833488 1101 14-15 0.1019618778395639 1101 16-17 0.3465715047739053 1101 18-19 0.3522345633857853 1101 20-21 0.12169448081811396 1101 22-23 0.1686588692564044 1101 24-25 0.33033538116123395 1101 26-27 -0.10886879518330517 1101 28-29 0.07610580462416294 1101 30-31 0.033519022842639856 1101 32-33 0.07250220192126022 1101 34-35 -0.03258554704429173 1101 36-37 -0.11825472577692864 1101 38-39 -0.10955038440599907 1101 40-41 -0.052757096178517315 1101 42-43 0.03385874188634119 1101 44-45 -0.3512381614570472 1101 46-47 -0.4691270620541914 1101 48-49 -0.2328439356071783 1101 50-51 -0.3722804533688233 1101 52-53 -0.20823157764893807 1101 54-55 -0.36030531371383745 1101 56-57 -0.3086070835257573 1101 58-59 -0.9050320209217873 1101 60-61 -0.6428331663476214 1101 62-63 -0.24236311178938053 1101 64-65 -0.4617226025972627 1101 66-67 -0.64817445053054 1101 68-69 -0.5354619981274524 1101 70-71 -0.3089253772399232 1101 72-73 0.19096756152165284 1101 74-75 0.05903151505695803 1101 76-77 -0.21918935666148798 1101 78-79 -0.5357269802625879 1101 80-81 -0.5604513500695738 1101 82-83 -0.6197011331472808 1101 84-85 -0.24087418823198092 1101 86-87 -0.39720050780206506 1101 88-89 -0.5182637806414903 1101 90-91 -0.9510778163598381 1101 92-93 -0.7556780661934823 1101 94-95 -0.9821181831779029 1101 96-97 -0.880104801776465 1101 98-99 -0.9781819880129845 1101 100-101 -1.0040042691254207 1102 1 0.1823887935272417 1102 2 0.23352137344935642 1102 3 0.24141218709119983 1102 4 0.12368009603567032 1102 5 -0.051566990033862226 1102 6 0.33551826153861697 1102 7 0.13566596024295308 1102 8 0.13625211963682204 1102 9 0.21291210775559222 1102 10-11 0.3019827507390218 1102 12-13 0.42113053036243997 1102 14-15 0.4210915986934296 1102 16-17 0.3987882535423779 1102 18-19 0.5207074697897198 1102 20-21 0.2634021983879009 1102 22-23 0.4865570630166687 1102 24-25 0.6523386652334864 1102 26-27 0.3252034544061857 1102 28-29 0.31206639575717077 1102 30-31 0.30708224123343797 1102 32-33 0.2895793776191269 1102 34-35 0.7492699537767322 1102 36-37 0.40580110345131004 1102 38-39 0.6529290901424432 1102 40-41 0.201183790520993 1102 42-43 0.5716255727566093 1102 44-45 0.4797142194953281 1102 46-47 0.5878515914761877 1102 48-49 1.0297997425537417 1102 50-51 1.1145011558265807 1102 52-53 0.7258406719405599 1102 54-55 1.0834549490119372 1102 56-57 1.0142172186088843 1102 58-59 0.8746888329369895 1102 60-61 0.40544269572134084 1102 62-63 0.4605925532352444 1102 64-65 0.7953381527393546 1102 66-67 0.789017667696065 1102 68-69 0.8871981003947198 1102 70-71 0.7211238838438234 1102 72-73 0.943348513902599 1102 74-75 0.7796390684231369 1102 76-77 0.7136513658344086 1102 78-79 0.7116457619377421 1102 80-81 0.6524386335100623 1102 82-83 0.5510706238313716 1102 84-85 0.25855109912434315 1102 86-87 0.5337518731503188 1102 88-89 0.6406032144323994 1102 90-91 0.593749769847058 1102 92-93 0.6265058418524951 1102 94-95 0.7533497971176644 1102 96-97 0.3713730307358496 1102 98-99 0.7599132500822563 1102 100-101 0.6957494254558618 1103 1 -0.09954206100635332 1103 2 0.015321764838791552 1103 3 0.2885093821335971 1103 4 0.1788660060161007 1103 5 0.3807226381461817 1103 6 -0.06337280173600846 1103 7 -0.19349255247720265 1103 8 0.003832028312615421 1103 9 0.032938200384421634 1103 10-11 -0.04374458128967973 1103 12-13 -0.16696470773280225 1103 14-15 -2.7174116306838414E-4 1103 16-17 0.08952537030689456 1103 18-19 -0.17805313034074288 1103 20-21 -0.38734796469103117 1103 22-23 -0.28721984500680975 1103 24-25 -0.2189594430509274 1103 26-27 -0.03842994415871459 1103 28-29 0.09230122941666252 1103 30-31 -0.00583361003857874 1103 32-33 -0.3607141644552385 1103 34-35 -0.07855131171577057 1103 36-37 -0.12195493046910855 1103 38-39 -0.3548660827212231 1103 40-41 -0.5030236458782227 1103 42-43 -0.15733724524729809 1103 44-45 -0.4993138300806592 1103 46-47 -0.3488085520332689 1103 48-49 -0.4605133689922454 1103 50-51 -0.4899672068609604 1103 52-53 -0.6110477820711822 1103 54-55 -0.6652404195464356 1103 56-57 -0.9497097220303843 1103 58-59 -0.7449784860453974 1103 60-61 -0.6413935730327367 1103 62-63 -0.34971403515353217 1103 64-65 -0.6527049000982217 1103 66-67 -0.8911845501773925 1103 68-69 -0.6811645871460499 1103 70-71 -0.25871956038317734 1103 72-73 -0.4727636844013787 1103 74-75 -0.5008240757386417 1103 76-77 -0.22679220885574125 1103 78-79 -0.10475997844060103 1103 80-81 -0.23311257327402402 1103 82-83 -0.31259539051957574 1103 84-85 -0.3924926060289522 1103 86-87 -0.46830292137022766 1103 88-89 -0.5663439479942163 1103 90-91 -0.42588493334928756 1103 92-93 -0.6658946800000827 1103 94-95 -0.24717205545898224 1103 96-97 -0.1270287957481706 1103 98-99 -0.23421590843046403 1103 100-101 -0.013350378849420252 1104 1 0.20808665888027988 1104 2 0.7142274489830172 1104 3 0.5574630902110655 1104 4 0.36452574463994125 1104 5 0.6284001692436476 1104 6 0.4514870628522445 1104 7 0.5772915760065018 1104 8 0.49257395871727994 1104 9 0.7023209747506627 1104 10-11 0.5080582844007608 1104 12-13 0.23964448766950142 1104 14-15 0.7004840453272507 1104 16-17 0.801312884084254 1104 18-19 0.7275424451591803 1104 20-21 0.5798225596358506 1104 22-23 0.6055431057096072 1104 24-25 0.8053969575159101 1104 26-27 0.7916853920088158 1104 28-29 0.25467723319559354 1104 30-31 0.4593195154534726 1104 32-33 0.5534135319705271 1104 34-35 0.826404600738968 1104 36-37 0.789072860429961 1104 38-39 0.5132944431802073 1104 40-41 0.6990901944618813 1104 42-43 0.21643263351194975 1104 44-45 0.3008554346020631 1104 46-47 0.6691118542019652 1104 48-49 0.5140772466588359 1104 50-51 0.368175211655803 1104 52-53 0.5109802450111616 1104 54-55 0.5057661148575932 1104 56-57 0.33117124159739575 1104 58-59 0.21134728942303838 1104 60-61 0.42428506025336077 1104 62-63 0.4488314694914024 1104 64-65 0.4705434072549579 1104 66-67 0.5331679140014742 1104 68-69 0.12648381468043368 1104 70-71 0.2877494996073722 1104 72-73 0.37662027088125427 1104 74-75 0.19018915052494378 1104 76-77 0.25217763841240526 1104 78-79 0.2650735123482484 1104 80-81 0.17107574352648314 1104 82-83 0.14415354665567293 1104 84-85 0.12027113196506534 1104 86-87 0.2933783099319278 1104 88-89 0.19109582526983715 1104 90-91 0.5446118388125747 1104 92-93 0.5648268599148913 1104 94-95 0.5850000434230864 1104 96-97 0.7821488928048161 1104 98-99 0.6261899331692824 1104 100-101 0.7549243023031522 1105 1 0.19627593428662848 1105 2 0.14279345232496254 1105 3 0.1740322835879482 1105 4 0.2051569158384794 1105 5 0.24134259670832847 1105 6 0.4230995495604333 1105 7 0.3610907557117997 1105 8 0.34476900656780174 1105 9 0.44783973519422915 1105 10-11 0.31635237524080395 1105 12-13 0.4791439559970101 1105 14-15 0.7161304071683574 1105 16-17 0.8281148740584285 1105 18-19 0.7962895969137023 1105 20-21 0.833278430819199 1105 22-23 0.8899973818754887 1105 24-25 0.5200122544929755 1105 26-27 0.5250041676430257 1105 28-29 0.5246529281674839 1105 30-31 0.8763501245982397 1105 32-33 0.5876494426495427 1105 34-35 0.9196181811734192 1105 36-37 0.8422496035561977 1105 38-39 0.6786161050365038 1105 40-41 0.8011523241899638 1105 42-43 0.6602557384792931 1105 44-45 0.7979122476052609 1105 46-47 0.8239329843857703 1105 48-49 1.2339428094721399 1105 50-51 1.0829614033617148 1105 52-53 1.3586181000924356 1105 54-55 1.2237423415019748 1105 56-57 1.1281672920303336 1105 58-59 1.0809925879191624 1105 60-61 1.2447126768415444 1105 62-63 1.1121973361175606 1105 64-65 0.9105783457328513 1105 66-67 0.7677778942536477 1105 68-69 0.9287965900108262 1105 70-71 0.9487513224844761 1105 72-73 0.6280348994700446 1105 74-75 0.9900334464386589 1105 76-77 1.1288391149428634 1105 78-79 1.2586459717132819 1105 80-81 1.1918869238394088 1105 82-83 1.3753836089373124 1105 84-85 1.1795810970265883 1105 86-87 1.0814023871003933 1105 88-89 1.217223730480228 1105 90-91 0.8893444835034465 1105 92-93 1.0141185962053392 1105 94-95 0.9019338122215075 1105 96-97 0.8892144404274767 1105 98-99 1.06429755887185 1105 100-101 1.1025146866264102 1106 1 -0.23303437979067 1106 2 -0.017972746465815703 1106 3 -0.21939367371465934 1106 4 0.16019445886582417 1106 5 0.13279776801510934 1106 6 0.007486644033313894 1106 7 0.1571812870214373 1106 8 0.10251392800389425 1106 9 0.314878562353627 1106 10-11 0.14187093273246631 1106 12-13 0.2230487875438527 1106 14-15 0.21605014891513719 1106 16-17 0.373166506868003 1106 18-19 0.19184685671857693 1106 20-21 0.22920131155543544 1106 22-23 0.420468765349419 1106 24-25 0.4525507338666017 1106 26-27 0.15042998226429205 1106 28-29 0.21680902155242165 1106 30-31 0.44515820036203024 1106 32-33 0.2100343612320117 1106 34-35 0.1098926643994389 1106 36-37 -0.10450350422171795 1106 38-39 0.24169909207034124 1106 40-41 -0.007404989120416872 1106 42-43 -0.015322566823101624 1106 44-45 0.12880810806290555 1106 46-47 -0.03102251687160873 1106 48-49 0.1528284682140466 1106 50-51 0.07761085314680116 1106 52-53 0.05453741379519528 1106 54-55 -0.21262178127531683 1106 56-57 -0.1010324440091992 1106 58-59 -0.17419298699746122 1106 60-61 -0.7000518097731643 1106 62-63 -0.7647400089394196 1106 64-65 -0.6333585891152822 1106 66-67 -0.6217689141431535 1106 68-69 -0.43373885738434836 1106 70-71 -0.4213284007137261 1106 72-73 -0.08173342170564268 1106 74-75 -0.20938156007117925 1106 76-77 -0.5940023839246074 1106 78-79 -0.5633590578039218 1106 80-81 -0.8336708710204945 1106 82-83 -0.8941771108900483 1106 84-85 -0.7355591765648803 1106 86-87 -0.6991091255001542 1106 88-89 -0.6258803020511188 1106 90-91 -0.5617158171974772 1106 92-93 -0.5706697895336639 1106 94-95 -0.7503517644786335 1106 96-97 -0.47660267776617715 1106 98-99 -0.17685173931431564 1106 100-101 0.0860617447155505 1107 1 -0.1367409273266169 1107 2 0.2702619317416435 1107 3 0.5057389522800335 1107 4 0.4378016067089092 1107 5 0.3482277554505373 1107 6 0.011831890438450898 1107 7 0.12039502428236659 1107 8 -0.054839834386172015 1107 9 0.17214856095755948 1107 10-11 0.09426518095248326 1107 12-13 0.07154103939363665 1107 14-15 0.0216047349824251 1107 16-17 0.23881288408425405 1107 18-19 0.24693899688331555 1107 20-21 0.10352945618757303 1107 22-23 0.174508622950988 1107 24-25 0.010138336826258865 1107 26-27 0.07185780580191903 1107 28-29 0.013297922850760813 1107 30-31 -0.034214967305146615 1107 32-33 -0.14055198527085366 1107 34-35 -0.02488850270931664 1107 36-37 -0.023427139570038946 1107 38-39 0.3387254776629689 1107 40-41 0.3068488151515325 1107 42-43 0.24229470247747287 1107 44-45 0.16507957253310224 1107 46-47 0.5074739231674883 1107 48-49 0.34381862596917756 1107 50-51 0.36817521165581013 1107 52-53 0.30623886570082703 1107 54-55 0.4626626665817284 1107 56-57 0.48634365539049895 1107 58-59 0.5281576342506256 1107 60-61 0.5988540257706063 1107 62-63 0.638486641905196 1107 64-65 0.49425030380667323 1107 66-67 0.4814437760704422 1107 68-69 0.6113976077838785 1107 70-71 0.32007708581426897 1107 72-73 0.2753271674329838 1107 74-75 0.0888960470766591 1107 76-77 0.3340741901365405 1107 78-79 0.5323148916585971 1107 80-81 0.6689205711126895 1107 82-83 0.47820527079360176 1107 84-85 0.33147802851678776 1107 86-87 0.4528610685526182 1107 88-89 0.5553199632008727 1107 90-91 0.6135773560539555 1107 92-93 0.5109475495700657 1107 94-95 0.6690517675610153 1107 96-97 0.7605971686668802 1107 98-99 0.9580864848934212 1107 100-101 1.1708725781652198 1108 1 -0.08315742496015588 1108 2 -0.1622769458783715 1108 3 0.10247661286150844 1108 4 -0.043266682675813684 1108 5 0.0949316094059185 1108 6 0.10890761728091292 1108 7 0.18104411150346067 1108 8 0.0068403549309365985 1108 9 0.11067121139704739 1108 10-11 -0.053199322090115686 1108 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60-61 -1.0574721671935166 2316 62-63 -1.2379283702856796 2316 64-65 -1.1512347188335141 2316 66-67 -1.1426201389417443 2316 68-69 -0.9732636600670403 2316 70-71 -1.0788039660840312 2316 72-73 -0.9888664933124041 2316 74-75 -0.6964792576951915 2316 76-77 -0.7007786485956089 2316 78-79 -0.8701363448443651 2316 80-81 -0.6510245699531403 2316 82-83 -0.5809640082032601 2316 84-85 -0.6453810798078372 2316 86-87 -0.24730873291028743 2316 88-89 -0.6425858187566327 2316 90-91 -0.8815027554054673 2316 92-93 -0.858523018176367 2316 94-95 -0.7972274034595372 2316 96-97 -0.6579198985570791 2316 98-99 -0.6149063747373695 2316 100-101 -0.7610326813262454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5470.0 3 592.0 4 408.0 5 375.0 6 398.0 7 410.0 8 434.0 9 494.0 10 517.0 11 611.0 12 788.0 13 1086.0 14 1363.0 15 1671.0 16 2301.0 17 3499.0 18 3236.0 19 1635.0 20 1334.0 21 1115.0 22 1165.0 23 1142.0 24 1291.0 25 1431.0 26 1571.0 27 1822.0 28 2154.0 29 2643.0 30 3202.0 31 4006.0 32 5028.0 33 6511.0 34 9368.0 35 15024.0 36 24018.0 37 49310.0 38 83325.0 39 27517.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.586219767094654 21.313451776649746 15.172812779934308 20.92751567632129 2 16.43114156887336 22.7111887485443 39.83509510585566 21.022574576726687 3 18.14524105446633 28.60480653312283 32.22668861294308 21.023263799467763 4 13.085995819647655 21.056285458345776 38.79665571812481 27.061063003881756 5 12.798990830139246 38.193944234408065 34.7066353664772 14.300429568975492 6 26.02579744861218 38.56632750148315 20.085742749364766 15.322132300539904 7 23.504212906644376 35.859709089280784 24.128499249960818 16.50757875411402 8 24.14815975642518 33.917271126235036 22.265380137906334 19.66918897943345 9 26.295740807067812 17.197573424068768 22.524176217765042 33.982509551098374 10-11 22.93339600715526 28.133084876396403 28.888341736475233 20.045177379973104 12-13 25.117765469019055 27.461881299869596 28.27341364083258 19.146939590278773 14-15 22.912130049989084 28.28796250165604 28.005642664815632 20.794264783539244 16-17 19.85264752975602 30.679023174049842 28.93751702876508 20.53081226742906 18-19 19.458460849443906 28.79040083600806 32.40818840038815 19.342949914159888 20-21 21.91725612611789 27.282378909697485 32.16461452057401 18.635750443610608 22-23 21.414177976632498 27.624925833740704 31.453770631280808 19.50712555834599 24-25 20.345721113575692 28.17089801301771 32.21593951186388 19.267441361542723 26-27 19.994626905769362 28.20258804038776 32.43054156312266 19.372243490720216 28-29 19.72738856830491 28.1480818132802 32.5874392025388 19.53709041587609 30-31 20.434915624801793 27.878326598987424 32.27915635994613 19.407601416264658 32-33 19.995187052050724 28.27924052427554 31.92234364448358 19.803228779190157 34-35 20.414465042689116 28.489574004418174 31.85973789479969 19.23622305809302 36-37 20.330245445131677 28.266771151101285 31.751886783651916 19.65109662011512 38-39 19.941124126236144 28.594907498092503 32.107125734316085 19.356842641355264 40-41 20.292147827546966 28.223606887674197 31.981978620599595 19.502266664179242 42-43 20.20356537896143 28.34338225966525 31.430628828976726 20.022423532396594 44-45 20.62921666965192 28.593498119290707 31.059876709057257 19.71740850200012 46-47 20.573281563976884 28.596536727074618 30.641625323947185 20.188556385001316 48-49 20.392172230339607 29.02458913912821 30.432162004303148 20.151076626229035 50-51 20.52786142611395 29.096747230276094 30.006306276050704 20.369085067559247 52-53 20.677889584102857 29.46855307193786 29.594475101017448 20.259082242941837 54-55 20.85577258490851 29.413125353260817 29.38234619280439 20.348755869026277 56-57 20.576601588154517 29.457915397934205 29.597475968714548 20.36800704519673 58-59 20.34997388254608 29.393142302813224 29.853928811282742 20.40295500335796 60-61 20.779148097088502 29.455999194100517 29.52763470367337 20.23721800513761 62-63 20.482851514111307 29.38301270092384 29.643635359610766 20.49050042535409 64-65 20.571941432540505 29.2232739784561 29.5197684767945 20.685016112208892 66-67 20.44580603873703 29.376852938487573 29.475738516679012 20.70160250609639 68-69 20.59929845510859 29.324016717665497 29.39417120680648 20.682513620419435 70-71 20.482482897061484 29.29597644859604 29.557720901684455 20.66381975265802 72-73 20.66089182336666 29.18117995608234 29.35692524109007 20.801002979460936 74-75 20.44787673596538 29.498120633854708 29.121314716882583 20.932687913297332 76-77 20.43980155568699 29.55656017418918 29.246281034910602 20.75735723521323 78-79 20.426253610263224 29.48493578022732 29.181561723373612 20.907248886135843 80-81 20.233821674590086 29.514482315976448 29.057175481935353 21.194520527498113 82-83 20.498309487024475 29.58920161513028 29.05965682213415 20.8528320757111 84-85 20.4218861362678 29.505722292499264 28.837592868310775 21.234798702922156 86-87 20.155844446642124 29.701876589559244 29.02770754535667 21.114571418441965 88-89 20.605772280367905 29.29852054998974 28.969608775955674 21.126098393686686 90-91 20.414035022250417 29.401337823843864 29.095522861487655 21.089104292418064 92-93 20.36638936474347 29.623160495485912 28.957337304386442 21.053112835384173 94-95 20.39093429453095 29.38201261945007 28.94376471553998 21.283288370478996 96-97 20.480779275044394 29.40375460745251 28.89880019698258 21.216665920520512 98-99 20.36712308323695 29.492034473752938 28.76748871394993 21.37335372906018 100-101 20.28673647827734 29.55397584650155 28.833775332525853 21.325512342695255 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1042.0 1 606.0 2 152.5 3 143.0 4 118.0 5 104.0 6 151.0 7 258.0 8 405.5 9 501.0 10 434.0 11 336.0 12 329.0 13 335.5 14 358.5 15 393.5 16 427.0 17 461.0 18 522.0 19 607.0 20 687.5 21 722.5 22 804.5 23 932.5 24 1039.0 25 1206.5 26 1607.5 27 2245.5 28 2783.0 29 3517.0 30 4584.5 31 5674.0 32 7073.5 33 8613.0 34 9644.0 35 10512.0 36 11486.5 37 12090.5 38 11694.0 39 10941.0 40 10469.0 41 10207.5 42 9820.0 43 9537.5 44 9650.5 45 9455.0 46 9090.0 47 8743.5 48 8426.5 49 8053.5 50 7607.0 51 7021.0 52 6402.5 53 5878.5 54 5236.0 55 4541.0 56 4074.5 57 3590.0 58 3085.5 59 2641.0 60 2149.5 61 1736.0 62 1320.0 63 981.0 64 733.5 65 555.5 66 464.0 67 356.5 68 258.0 69 219.0 70 187.5 71 144.0 72 116.0 73 101.0 74 90.5 75 71.0 76 53.0 77 48.5 78 37.5 79 25.0 80 22.0 81 18.5 82 14.0 83 8.5 84 6.5 85 6.0 86 6.0 87 4.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13716603053435114 2 0.14014790076335878 3 0.13455689408396948 4 0.13716603053435114 5 0.1285931536259542 6 0.1032472566793893 7 0.11256560114503818 8 0.10436545801526717 9 0.09541984732824427 10-11 0.0870333373091603 12-13 0.10306088979007634 14-15 0.1228157800572519 16-17 0.13381142652671754 18-19 0.12971135496183206 20-21 0.11797024093511452 22-23 0.11517473759541984 24-25 0.10007901956106871 26-27 0.10660186068702289 28-29 0.10716096135496182 30-31 0.09653804866412215 32-33 0.09728351622137404 34-35 0.11331106870229007 36-37 0.11387016937022901 38-39 0.09933355200381679 40-41 0.10175632156488548 42-43 0.09914718511450382 44-45 0.11331106870229007 46-47 0.11349743559160305 48-49 0.12840678673664122 50-51 0.11219286736641222 52-53 0.09877445133587787 54-55 0.09299707776717557 56-57 0.11331106870229007 58-59 0.09989265267175572 60-61 0.09858808444656489 62-63 0.10287452290076336 64-65 0.12057937738549618 66-67 0.11237923425572519 68-69 0.1148020038167939 70-71 0.10380635734732824 72-73 0.10678822757633588 74-75 0.09150614265267176 76-77 0.11312470181297711 78-79 0.11256560114503818 80-81 0.11405653625954199 82-83 0.12002027671755724 84-85 0.11219286736641222 86-87 0.12244304627862596 88-89 0.10548365935114505 90-91 0.11759750715648855 92-93 0.13008408874045801 94-95 0.10772006202290077 96-97 0.09094704198473283 98-99 0.09616531488549618 100-101 0.14033426765267176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.46391911805673 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70415794781619 45.30146402769052 2 16.471063579061497 20.247522392236117 3 5.1311217426727955 9.461365553296293 4 1.8888693281315379 4.6438924643542006 5 0.8782626670356098 2.699073276554276 6 0.45106806752714684 1.6634646731536025 7 0.29244436054486006 1.258234356614277 8 0.21997346544491508 1.0816345030579928 9 0.14014182176845616 0.7752299038410162 >10 0.7782723557624512 8.587461679469577 >50 0.034232619138868436 1.3883110894549382 >100 0.007335561244043236 0.708996759350871 >500 0.001833890311010809 0.7942867775663707 >1k 0.0012225935406738728 1.3890625433599206 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1855 0.691421159351145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1842 0.6865756202290076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 818 0.3048962309160306 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 785 0.29259601622137404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 511 0.19046696087786258 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 351 0.13082955629770993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.591006679389313E-4 0.0 24-25 0.0 0.0 0.0 0.002050035782442748 0.0 26-27 0.0 0.0 0.0 0.003540970896946565 0.0 28-29 0.0 0.0 0.0 0.0061501073473282444 0.0 30-31 0.0 0.0 0.0 0.011741114026717556 0.0 32-33 0.0 0.0 0.0 0.02050035782442748 0.0 34-35 0.0 0.0 0.0 0.03652791030534351 0.0 36-37 0.0 0.0 0.0 0.05423276479007634 0.0 38-39 0.0 0.0 0.0 0.06970121660305342 0.0 40-41 0.0 0.0 0.0 0.08013776240458015 0.0 42-43 0.0 0.0 0.0 0.09541984732824427 0.0 44-45 0.0 0.0 0.0 0.11275196803435114 0.0 46-47 0.0 0.0 0.0 0.13064318940839692 0.0 48-49 0.0 0.0 0.0 0.15487088501908397 0.0 50-51 0.0 0.0 0.0 0.17574397662213742 0.0 52-53 0.0 0.0 0.0 0.1992262046755725 0.0 54-55 0.0 0.0 0.0 0.24376789122137404 0.0 56-57 0.0 0.0 0.0 0.300796159351145 0.0 58-59 0.0 0.0 0.0 0.3596880963740458 0.0 60-61 0.0 0.0 0.0 0.4111253578244275 0.0 62-63 0.0 0.0 0.0 0.48399481154580154 0.0 64-65 0.0 0.0 0.0 0.5782964575381679 0.0 66-67 0.0 0.0 0.0 0.6966394322519084 0.0 68-69 0.0 0.0 0.0 0.8313826932251909 0.0 70-71 0.0 0.0 0.0 0.9629577170801527 0.0 72-73 0.0 0.0 0.0 1.1086966245229006 0.0 74-75 0.0 0.0 0.0 1.2730722208969465 0.0 76-77 0.0 0.0 0.0 1.4495616650763359 0.0 78-79 0.0 0.0 0.0 1.6307102814885497 0.0 80-81 0.0 0.0 0.0 1.8161453363549618 0.0 82-83 0.0 0.0 0.0 2.027112655057252 0.0 84-85 0.0 0.0 0.0 2.254480260019084 0.0 86-87 0.0 0.0 0.0 2.522475846851145 0.0 88-89 0.0 0.0 0.0 2.831472149332061 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1020 0.0 62.39583 1 GCGTCAG 75 1.3586032E-7 44.32898 1 TGGTATC 370 0.0 41.077045 2 CGTCAGA 85 3.629575E-7 39.113804 2 GTATCAA 1810 0.0 37.786343 1 GTGGTAT 475 0.0 32.996758 1 TATCAAC 2150 0.0 31.148102 2 ATCAACG 2185 0.0 30.649162 3 TCAACGC 2210 0.0 30.30245 4 CAACGCA 2250 0.0 29.552652 5 TATAGAC 65 0.005838981 29.227896 2 AACGCAG 2325 0.0 28.59934 6 CTACACG 70 0.008384304 27.14019 4 GAGTACT 1495 0.0 25.738062 12-13 ACGCAGA 2645 0.0 24.95974 7 CGCAGAG 2670 0.0 24.726034 8 CATGGGG 855 0.0 23.886541 4 ACATGGG 1930 0.0 23.870712 3 GTACATG 2115 0.0 23.803806 1 TACATGG 2080 0.0 23.519323 2 >>END_MODULE