FastQCFastQC Report
Fri 10 Feb 2017
SRR2031696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268288
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT27661.0309816316793892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.9143159589694657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23530.8770425811068702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.4059070849236642No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT9800.3652791030534351RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG7890.29408695133587787RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT7840.2922232824427481RNA PCR Primer, Index 23 (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.24861343034351144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.22923127385496184No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.19382156488549618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1162929389312977No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT2900.10809279580152671RNA PCR Primer, Index 23 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.054.83941
TACTTCG459.6131617E-442.221067
TGGTATC4300.041.975592
GTATCAA19100.041.281591
GTGGTAT4550.040.713171
TATCAAC24100.032.9140362
ATCAACG24550.032.3107223
TCAACGC24550.032.3107224
CAACGCA24800.031.985015
AACGCAG25850.030.685816
TGTATCG650.005836975829.2299674
ACGCAGA28950.027.2358677
GACGCCA350.00838877627.1421152-53
CGCAGAG29350.026.864688
GCAGAGT31100.025.2002729
ACATCTC12150.024.2380168
GAGTACT18500.024.13447212-13
CTTATAC12050.024.0449831
TATACAC12400.023.749353
ACACATC12600.023.3723746
Produced by FastQC (version 0.11.5)