Basic Statistics
Measure | Value |
---|---|
Filename | SRR2031696_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 268288 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT | 2766 | 1.0309816316793892 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2453 | 0.9143159589694657 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2353 | 0.8770425811068702 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1089 | 0.4059070849236642 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT | 980 | 0.3652791030534351 | RNA PCR Primer, Index 23 (95% over 23bp) |
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG | 789 | 0.29408695133587787 | RNA PCR Primer, Index 23 (96% over 30bp) |
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT | 784 | 0.2922232824427481 | RNA PCR Primer, Index 23 (96% over 25bp) |
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 667 | 0.24861343034351144 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 615 | 0.22923127385496184 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 520 | 0.19382156488549618 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 312 | 0.1162929389312977 | No Hit |
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT | 290 | 0.10809279580152671 | RNA PCR Primer, Index 23 (96% over 28bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 1100 | 0.0 | 54.8394 | 1 |
TACTTCG | 45 | 9.6131617E-4 | 42.22106 | 7 |
TGGTATC | 430 | 0.0 | 41.97559 | 2 |
GTATCAA | 1910 | 0.0 | 41.28159 | 1 |
GTGGTAT | 455 | 0.0 | 40.71317 | 1 |
TATCAAC | 2410 | 0.0 | 32.914036 | 2 |
ATCAACG | 2455 | 0.0 | 32.310722 | 3 |
TCAACGC | 2455 | 0.0 | 32.310722 | 4 |
CAACGCA | 2480 | 0.0 | 31.98501 | 5 |
AACGCAG | 2585 | 0.0 | 30.68581 | 6 |
TGTATCG | 65 | 0.0058369758 | 29.229967 | 4 |
ACGCAGA | 2895 | 0.0 | 27.235867 | 7 |
GACGCCA | 35 | 0.008388776 | 27.14211 | 52-53 |
CGCAGAG | 2935 | 0.0 | 26.86468 | 8 |
GCAGAGT | 3110 | 0.0 | 25.200272 | 9 |
ACATCTC | 1215 | 0.0 | 24.238016 | 8 |
GAGTACT | 1850 | 0.0 | 24.134472 | 12-13 |
CTTATAC | 1205 | 0.0 | 24.044983 | 1 |
TATACAC | 1240 | 0.0 | 23.74935 | 3 |
ACACATC | 1260 | 0.0 | 23.372374 | 6 |