##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031696_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268288 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66357421875 34.0 31.0 34.0 31.0 34.0 2 32.82384974355916 34.0 31.0 34.0 31.0 34.0 3 32.84528193583015 34.0 31.0 34.0 31.0 34.0 4 36.287101920324424 37.0 37.0 37.0 35.0 37.0 5 36.26507335400763 37.0 37.0 37.0 35.0 37.0 6 36.317177808921755 37.0 37.0 37.0 35.0 37.0 7 36.26762658039122 37.0 37.0 37.0 35.0 37.0 8 36.267250119274806 37.0 37.0 37.0 35.0 37.0 9 38.08625805104962 39.0 39.0 39.0 35.0 39.0 10-11 38.058455838501914 39.0 39.0 39.0 35.0 39.0 12-13 37.99211108957539 39.0 39.0 39.0 35.0 39.0 14-15 39.37228463442271 41.0 39.0 41.0 36.0 41.0 16-17 39.324192658635496 41.0 39.0 41.0 36.0 41.0 18-19 39.41566898258588 41.0 39.0 41.0 36.0 41.0 20-21 39.46747152313931 41.0 39.0 41.0 36.0 41.0 22-23 39.362666239265266 41.0 39.0 41.0 36.0 41.0 24-25 39.27681819537214 41.0 39.0 41.0 36.0 41.0 26-27 39.10881217199427 41.0 39.0 41.0 35.0 41.0 28-29 38.98584916209447 40.5 39.0 41.0 35.0 41.0 30-31 38.74542655653626 40.0 38.0 41.0 35.0 41.0 32-33 38.413734494274806 40.0 38.0 41.0 35.0 41.0 34-35 38.19534604604007 40.0 38.0 41.0 34.0 41.0 36-37 38.051329168654576 40.0 37.5 41.0 34.0 41.0 38-39 38.00902015744275 40.0 38.0 41.0 34.0 41.0 40-41 37.909408918773856 40.0 38.0 41.0 34.0 41.0 42-43 37.73656667461832 40.0 38.0 41.0 33.0 41.0 44-45 37.410059339217554 40.0 37.0 41.0 33.0 41.0 46-47 36.965615308921755 40.0 37.0 41.0 31.5 41.0 48-49 36.50658434219943 40.0 37.0 41.0 30.5 41.0 50-51 36.16187268905057 40.0 37.0 41.0 29.0 41.0 52-53 36.197994319537216 40.0 37.0 41.0 29.0 41.0 54-55 36.12133975429389 40.0 37.0 41.0 29.0 41.0 56-57 35.86277246839218 40.0 36.0 41.0 28.0 41.0 58-59 35.63738780713263 40.0 36.0 41.0 27.0 41.0 60-61 35.319432848282446 39.5 35.0 41.0 26.0 41.0 62-63 34.98928949487119 39.0 35.0 41.0 24.0 41.0 64-65 34.59703564825858 39.0 35.0 41.0 21.5 41.0 66-67 34.11914248866889 38.5 35.0 40.5 13.5 41.0 68-69 33.576199830033396 37.5 35.0 40.0 4.0 41.0 70-71 33.19029923067748 37.0 34.0 39.5 2.0 41.0 72-73 32.7901192002624 36.5 34.0 39.0 2.0 41.0 74-75 32.39206002504771 36.0 34.0 39.0 2.0 40.5 76-77 31.47369244990458 35.0 32.5 37.0 2.0 39.0 78-79 31.63986835042939 35.0 33.0 37.0 2.0 39.0 80-81 31.392046979365457 35.0 34.0 37.0 2.0 39.0 82-83 31.091990696564885 35.0 33.0 36.0 2.0 38.5 84-85 30.763545145515266 35.0 33.0 36.0 2.0 37.0 86-87 30.49435308325382 35.0 33.0 36.0 2.0 37.0 88-89 30.250970971493324 35.0 33.0 35.0 2.0 36.5 90-91 30.02831472149332 35.0 33.0 35.0 2.0 36.0 92-93 29.83927160364981 35.0 32.0 35.0 2.0 36.0 94-95 29.65036826097328 35.0 32.0 35.0 2.0 36.0 96-97 29.470283799499047 35.0 32.0 35.0 2.0 35.5 98-99 29.25600846851145 35.0 32.0 35.0 2.0 35.0 100-101 28.613189930224237 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10025160874958772 1101 2 -0.009039185224253288 1101 3 0.12777802969259966 1101 4 -0.0893255070218899 1101 5 -0.13368027793635662 1101 6 -0.24650295417285406 1101 7 -0.08453183899027295 1101 8 -0.12253068158370439 1101 9 0.053540588331479455 1101 10-11 -0.20412492688071637 1101 12-13 0.05014652314305579 1101 14-15 0.17423076171714058 1101 16-17 0.06886777894866469 1101 18-19 0.05464214433816039 1101 20-21 -0.0048859375237739755 1101 22-23 -0.3442560501149785 1101 24-25 -0.03590387937125428 1101 26-27 0.07474886543014492 1101 28-29 -0.009327767023769695 1101 30-31 0.3579656798770898 1101 32-33 0.38753293899952723 1101 34-35 -0.16661835565217586 1101 36-37 0.041797944907926876 1101 38-39 0.05370415223568159 1101 40-41 0.12390162335230315 1101 42-43 0.019687177904152975 1101 44-45 0.0653052970665513 1101 46-47 0.10523093286210639 1101 48-49 -0.01039204126868043 1101 50-51 0.05357788691781451 1101 52-53 0.22823374844417543 1101 54-55 0.36431808682907985 1101 56-57 0.03192975607666426 1101 58-59 0.3225329219059958 1101 60-61 0.3562167959309974 1101 62-63 0.6232475941597713 1101 64-65 0.4537114035468406 1101 66-67 0.4631708496235092 1101 68-69 0.4938564966606762 1101 70-71 0.7612727424377894 1101 72-73 0.8334937702455676 1101 74-75 0.7455290624233513 1101 76-77 0.6466931196415153 1101 78-79 0.5809256336099047 1101 80-81 0.40569403034331586 1101 82-83 0.6839645633135518 1101 84-85 1.1097154381537742 1101 86-87 0.982445960721865 1101 88-89 0.6474727484940779 1101 90-91 0.5814644794171429 1101 92-93 0.6095121384101674 1101 94-95 0.8441245060378044 1101 96-97 0.8226510286956099 1101 98-99 -0.11695663451301286 1101 100-101 0.12145877053239218 1102 1 0.13160389207143197 1102 2 0.005082325448981351 1102 3 -0.06795267638293723 1102 4 -0.03990021966556867 1102 5 0.08027702912439594 1102 6 -0.05914663233377837 1102 7 0.041740739006442595 1102 8 0.14347917063304294 1102 9 -0.02347090592139267 1102 10-11 -0.0073268973240629975 1102 12-13 0.12338133430890252 1102 14-15 -0.3368529821252224 1102 16-17 0.006470406206467771 1102 18-19 0.1421626697897196 1102 20-21 0.10972818398689554 1102 22-23 0.19162243921179112 1102 24-25 0.051534544274062455 1102 26-27 0.28353375869779995 1102 28-29 0.15430113281203006 1102 30-31 0.20985402142060394 1102 32-33 0.3496019045167742 1102 34-35 0.28305323712287134 1102 36-37 0.4729966312790239 1102 38-39 0.4472181095427459 1102 40-41 0.27431213238349983 1102 42-43 0.0655820875921691 1102 44-45 0.14551876176278 1102 46-47 0.041027320382632126 1102 48-49 0.11235015905973 1102 50-51 0.41170596573554263 1102 52-53 0.6438330916297303 1102 54-55 0.7861571672888488 1102 56-57 0.8908788529567957 1102 58-59 0.7006117396399887 1102 60-61 0.7911100635828845 1102 62-63 0.6202098273289067 1102 64-65 0.7829396465681882 1102 66-67 0.9079984358304003 1102 68-69 0.8351537051992608 1102 70-71 0.5494500823392627 1102 72-73 0.7759404040715125 1102 74-75 0.9157261067583278 1102 76-77 1.1760855662753364 1102 78-79 0.9592507567626178 1102 80-81 0.7628368874861771 1102 82-83 0.7416821331000847 1102 84-85 0.7019978683672399 1102 86-87 0.9698022825609485 1102 88-89 0.8078175760802857 1102 90-91 1.0228437897619713 1102 92-93 1.0438306934183785 1102 94-95 0.7907583319819729 1102 96-97 0.6954753308302557 1102 98-99 0.6818939401996325 1102 100-101 0.7649727278394565 1103 1 0.3022496846350293 1103 2 0.1608553195781397 1103 3 0.172360435163057 1103 4 0.0672752532633325 1103 5 0.10173821633900815 1103 6 0.17588272187366272 1103 7 0.02784641415321687 1103 8 0.057373495486267245 1103 9 0.10523098579420065 1103 10-11 0.03510624031455478 1103 12-13 0.10074597879683012 1103 14-15 0.05828726575474974 1103 16-17 -0.1712856277139494 1103 18-19 0.17210396137485162 1103 20-21 0.20131331118846418 1103 22-23 0.27417299368014625 1103 24-25 0.24086918223883913 1103 26-27 0.2743687228204337 1103 28-29 0.1536162013051765 1103 30-31 0.15923432148583316 1103 32-33 0.35951710347306687 1103 34-35 0.06488624429833578 1103 36-37 -0.022893779679876047 1103 38-39 -0.27949421922437523 1103 40-41 0.23602119957201495 1103 42-43 0.2125814352764479 1103 44-45 0.7577496815279474 1103 46-47 0.30958570263964447 1103 48-49 0.03684461437610764 1103 50-51 -0.20094895924814438 1103 52-53 -0.06634303361489202 1103 54-55 -0.2769869944854477 1103 56-57 0.038171742715441326 1103 58-59 0.36711532737646024 1103 60-61 0.11273432972770081 1103 62-63 0.6547499447457383 1103 64-65 0.8122938540045865 1103 66-67 0.5819710385701313 1103 68-69 0.44640615138321493 1103 70-71 0.17697780575739586 1103 72-73 0.42261359389538455 1103 74-75 0.677598252877047 1103 76-77 0.794937490606717 1103 78-79 0.5823427332792441 1103 80-81 0.2608799403237505 1103 82-83 0.627168108312091 1103 84-85 0.643354685066523 1103 86-87 0.34840632691841833 1103 88-89 0.4901072042603225 1103 90-91 0.3309977362720815 1103 92-93 0.0979728982455157 1103 94-95 0.006348677709308248 1103 96-97 -0.0017523273563071484 1103 98-99 0.084698897799111 1103 100-101 0.040380425164961764 1104 1 0.314157258245487 1104 2 0.18923667684471468 1104 3 0.19920660112116906 1104 4 -0.023438807514502003 1104 5 0.2136103624577288 1104 6 0.030590641886256265 1104 7 0.2251972250491363 1104 8 0.1631836041305803 1104 9 -0.019119510190684252 1104 10-11 0.21798098444933345 1104 12-13 0.08647657240413764 1104 14-15 0.09048691935260678 1104 16-17 0.09534561146098497 1104 18-19 0.23136628226919953 1104 20-21 0.26789731370775627 1104 22-23 0.06553459027911401 1104 24-25 0.12634981521002686 1104 26-27 0.10553704277005238 1104 28-29 0.10027814430628013 1104 30-31 -0.09708358120665395 1104 32-33 0.2551437764379543 1104 34-35 0.12279051134289887 1104 36-37 0.25115755081925784 1104 38-39 0.5490859256346994 1104 40-41 0.43763315044589746 1104 42-43 0.2385911187908505 1104 44-45 0.19831022317492852 1104 46-47 0.29242305108871136 1104 48-49 0.4551252001106363 1104 50-51 0.5600434041591882 1104 52-53 0.6962140440106808 1104 54-55 0.662963407026119 1104 56-57 0.90939281026386 1104 58-59 0.7560304588518179 1104 60-61 0.8500591604630117 1104 62-63 1.1611170522878567 1104 64-65 1.2098690390148263 1104 66-67 1.09894671169247 1104 68-69 1.4144229991237296 1104 70-71 1.5676151069698037 1104 72-73 1.1826317012800516 1104 74-75 1.2510709343445328 1104 76-77 1.5087620851587538 1104 78-79 1.5485980474030079 1104 80-81 1.3933787594073586 1104 82-83 1.2341492923775945 1104 84-85 1.2759715957892404 1104 86-87 1.2382957144164486 1104 88-89 1.3469801376566402 1104 90-91 1.4463043808949756 1104 92-93 1.341654995553025 1104 94-95 1.58180923510184 1104 96-97 1.6884145754936384 1104 98-99 1.492341395043642 1104 100-101 1.359759263143232 1105 1 0.4601962981968768 1105 2 0.2522398668796555 1105 3 0.416964462278699 1105 4 0.22099761964636855 1105 5 0.13651785144451623 1105 6 0.17091420257287382 1105 7 0.26311686654997146 1105 8 0.25675796232592774 1105 9 0.16799122959361057 1105 10-11 0.2973615859987788 1105 12-13 0.39539727724995544 1105 14-15 0.4701010770314724 1105 16-17 0.5484255142408685 1105 18-19 0.4162553905784776 1105 20-21 0.337565398167726 1105 22-23 0.41303003308634345 1105 24-25 0.43400779789268995 1105 26-27 0.41098288812931116 1105 28-29 0.28594577304696855 1105 30-31 0.49548439691881896 1105 32-33 0.5749637673235668 1105 34-35 0.6661977724700421 1105 36-37 0.8975193919251367 1105 38-39 1.012049240232912 1105 40-41 0.9304270893795135 1105 42-43 0.6809901010177981 1105 44-45 0.5629615979280871 1105 46-47 0.6190218662185885 1105 48-49 0.9456310485080195 1105 50-51 1.4154189927965959 1105 52-53 1.3878439999578163 1105 54-55 1.37128408149065 1105 56-57 1.5508746574459948 1105 58-59 1.2284594425978312 1105 60-61 1.3860230067337156 1105 62-63 1.3924040794790997 1105 64-65 1.4010432756850335 1105 66-67 1.4159279512489107 1105 68-69 1.3580087503010034 1105 70-71 1.2769516556558145 1105 72-73 1.251008581122072 1105 74-75 1.4489860186525974 1105 76-77 1.5385357445845536 1105 78-79 1.6820953130873484 1105 80-81 1.5992119661520086 1105 82-83 1.2295256405471235 1105 84-85 1.4238145245451328 1105 86-87 1.2444194422001367 1105 88-89 1.2875847252306905 1105 90-91 1.5956568797556798 1105 92-93 1.553165704956033 1105 94-95 1.6141273271571386 1105 96-97 1.8642082865676173 1105 98-99 1.734337825242637 1105 100-101 1.714479755320049 1106 1 0.05726419667910676 1106 2 0.11269751727470378 1106 3 -0.03274928328480087 1106 4 0.12044294594075922 1106 5 0.09417716407715915 1106 6 0.16597386120776747 1106 7 -0.025099438360044246 1106 8 0.022600049753918938 1106 9 0.15325938133521078 1106 10-11 0.17523527734337563 1106 12-13 0.2043915521612263 1106 14-15 0.2786762255092441 1106 16-17 0.25912126412048764 1106 18-19 0.14671250591850793 1106 20-21 0.13452093509543772 1106 22-23 0.11353298480654672 1106 24-25 0.23560047834000386 1106 26-27 0.316028456981968 1106 28-29 0.24222286020744122 1106 30-31 0.4230022766741186 1106 32-33 0.4725631537938142 1106 34-35 0.3634368885591499 1106 36-37 0.5439817864747098 1106 38-39 0.7159765440906511 1106 40-41 0.5931284038313507 1106 42-43 0.7435558681975252 1106 44-45 0.6625710077623879 1106 46-47 0.6267801640302153 1106 48-49 0.5622730431931373 1106 50-51 0.6875301415597193 1106 52-53 0.6658496715410465 1106 54-55 0.19589304544577857 1106 56-57 0.365469174915539 1106 58-59 0.12759265346301163 1106 60-61 0.42904143046161636 1106 62-63 0.25015970201562254 1106 64-65 0.065376507952422 1106 66-67 0.018524751619878543 1106 68-69 -0.10817577234073639 1106 70-71 -0.11885529649243409 1106 72-73 -0.08281224678640342 1106 74-75 0.31066537801338967 1106 76-77 -0.02676772078847378 1106 78-79 -0.12310898875502829 1106 80-81 -0.21258243003840605 1106 82-83 -0.44541059872948807 1106 84-85 -0.3705103251935853 1106 86-87 -0.31502517068755864 1106 88-89 -0.09452481836737547 1106 90-91 -0.3683071645468772 1106 92-93 -0.4054656242989907 1106 94-95 -0.12875198226518236 1106 96-97 -0.16670897609089508 1106 98-99 -7.742687843652618E-4 1106 100-101 0.0011979061698959015 1107 1 -0.060842741754512986 1107 2 -0.009039185224253288 1107 3 0.22937901491427226 1107 4 0.049837054554465965 1107 5 -0.131217223749168 1107 6 -0.3228576339758149 1107 7 -0.08945794736465729 1107 8 -0.267850878628046 1107 9 0.07570807601621254 1107 10-11 0.11668788100106298 1107 12-13 0.008890365507582487 1107 14-15 0.12712485038709076 1107 16-17 0.08025940456443692 1107 18-19 0.21628007537264438 1107 20-21 0.004966279224994707 1107 22-23 0.27674148683083644 1107 24-25 0.22979809107209803 1107 26-27 -0.0043767503333995705 1107 28-29 -0.11739426948682308 1107 30-31 -0.028118063965273166 1107 32-33 0.4275575695414062 1107 34-35 0.4202043044463508 1107 36-37 0.3287437577158059 1107 38-39 0.31417213253125453 1107 40-41 0.40315039182520707 1107 42-43 0.5058324981011992 1107 44-45 0.5151205680025157 1107 46-47 0.9325092579852594 1107 48-49 0.9723665794209779 1107 50-51 1.3596123696764337 1107 52-53 1.5173347336658551 1107 54-55 1.184515131164055 1107 56-57 1.1335307412983369 1107 58-59 1.2538752864380243 1107 60-61 1.1323867466699156 1107 62-63 1.0512032591844047 1107 64-65 0.9340069700493032 1107 66-67 1.0342915392786836 1107 68-69 0.6062333439513097 1107 70-71 0.5568392449008428 1107 72-73 0.37444204610763876 1107 74-75 0.5765019688272943 1107 76-77 0.5712620851587538 1107 78-79 0.9537704611961111 1107 80-81 0.9106201387177002 1107 82-83 0.9819941199638009 1107 84-85 1.0949371130306211 1107 86-87 0.8309681282095518 1107 88-89 0.9655146204152594 1107 90-91 0.8191492084811856 1107 92-93 1.042086030035783 1107 94-95 1.1723264764811567 1107 96-97 1.1582421617005352 1107 98-99 1.2725138088367451 1107 100-101 1.4287247803846128 1108 1 -0.3630739790770292 1108 2 -0.2907159938787487 1108 3 -0.004647354254082359 1108 4 -0.018891816304218878 1108 5 0.11361036245772738 1108 6 0.09127353573344976 1108 7 0.09580236365630412 1108 8 0.10706460480670899 1108 9 0.180170550661245 1108 10-11 -0.19766303177784295 1108 12-13 -0.07773911387037202 1108 14-15 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sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 6.0 6 9.0 7 27.0 8 71.0 9 118.0 10 241.0 11 447.0 12 691.0 13 966.0 14 1234.0 15 1729.0 16 2469.0 17 3760.0 18 4027.0 19 2252.0 20 1548.0 21 1598.0 22 1878.0 23 1954.0 24 2280.0 25 2577.0 26 2143.0 27 1644.0 28 2014.0 29 2457.0 30 3042.0 31 3883.0 32 4761.0 33 6391.0 34 8945.0 35 13857.0 36 22171.0 37 48863.0 38 88634.0 39 29571.0 40 25.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.36201972904255 21.72580657187381 15.547991678907222 21.364182020176415 2 15.800930343511451 22.379308802480917 40.95785126431298 20.86190958969466 3 17.459613391878694 28.76525447104166 33.4435407518954 20.331591385184247 4 12.115044082834721 21.78829055527596 38.30863576208317 27.788029599806148 5 11.851815958969466 38.407234017175576 35.658695133587784 14.082254890267176 6 25.344033277671755 39.125492008587784 19.989712547709924 15.540762166030534 7 22.561948354007633 36.14473998091603 23.651076455152673 17.642235209923665 8 23.700650047709924 34.393636688931295 22.578721374045802 19.32699188931298 9 25.472999165076338 16.565407323473284 22.26003399332061 35.70155951812977 10-11 22.381731572041986 27.996965947041986 29.297806834446565 20.323495646469468 12-13 24.695895261613007 27.015235521595304 28.60942086381214 19.679448352979545 14-15 22.496058325599076 27.889349838046567 27.9089184343632 21.705673401991152 16-17 19.702035506752097 30.90421824389553 28.975872852579514 20.41787339677286 18-19 19.184421218988547 27.736052302003817 33.022162750477094 20.057363728530532 20-21 21.190474415553435 26.53119036259542 32.81044996421756 19.46788525763359 22-23 20.685792879293892 27.70753816793893 32.27557699188932 19.331091960877863 24-25 20.27615845658397 28.140654818702288 32.95823890744275 18.624947817270993 26-27 19.815496435726597 28.43796300610353 33.18697293015887 18.559567628010996 28-29 19.17203898260544 27.332554277630848 33.60861210667472 19.886794633088993 30-31 20.368782800572518 27.528998687977097 33.38315541507634 18.719063096374043 32-33 20.154722368210162 27.74361038738366 33.35513834065758 18.746528903748597 34-35 19.89402033109793 28.051621753603794 33.483126381593905 18.57123153370437 36-37 20.048606296453677 27.569163330575 33.071664467455406 19.31056590551592 38-39 19.111551765267176 27.490234375 33.67034679150763 19.72786706822519 40-41 19.336123866889313 27.198383826335874 33.956047232824424 19.50944507395038 42-43 19.865964933206108 27.2216796875 33.38911915553435 19.52323622375954 44-45 19.660774988072518 27.86334088740458 33.01116710400763 19.46471702051527 46-47 20.253980796755723 27.56701753339695 31.900420443702288 20.278581226145036 48-49 19.91311575620229 27.862222686068705 31.898929508587788 20.32573204914122 50-51 19.82235508110687 28.228619990458014 31.571296517175572 20.37772841125954 52-53 19.868760436545802 28.843630725190838 31.64677510734733 19.64083373091603 54-55 20.104328184637403 28.831889611164126 30.70934965410305 20.35443255009542 56-57 20.513030772900763 29.48976473043893 29.8095703125 20.187634184160306 58-59 19.50355402982627 29.902306112484016 31.040452947776075 19.553686909913637 60-61 20.247986253219572 30.396114521714175 29.848740686071697 19.507158538994563 62-63 19.64150918779484 30.936560508604412 29.854788295836 19.567142007764755 64-65 19.67206087635562 30.77085076682514 29.80749697612396 19.74959138069528 66-67 19.58412232178801 30.765331226050783 29.64968912612384 20.000857326037362 68-69 19.801736621694914 30.59402600645968 29.54176521128152 20.06247216056389 70-71 19.734240320551645 30.22093835903648 29.863672366397985 20.181148954013885 72-73 19.84500776265835 29.90627032688899 29.752321808317323 20.496400102135336 74-75 19.867745921575715 29.943359618893545 29.49754260143176 20.691351858098976 76-77 19.817543074930345 29.919021926514027 29.75184739966267 20.51158759889296 78-79 19.808859268536327 29.810946608817524 29.568852410132546 20.811341712513606 80-81 19.716359380009095 29.870611566304582 29.458141667474337 20.954887386211986 82-83 19.992619004572116 29.876629270865756 29.361637089874804 20.769114634687323 84-85 19.871701685918868 29.571605890838338 29.327458895710095 21.2292335275327 86-87 19.66259258016781 29.78779712316564 29.34945076244684 21.20015953421972 88-89 20.075144110077176 29.418210891795837 29.327449032650044 21.179195965476946 90-91 19.885719823473284 29.388567509541986 29.447645813454198 21.278066853530532 92-93 19.934734315362597 29.53486551765267 29.203132454675572 21.327267712309162 94-95 19.918334029103054 29.297806834446565 29.182072996183205 21.601786140267176 96-97 20.028663227576335 29.20536885734733 29.05907084923664 21.706897065839694 98-99 19.97409495410707 29.31593905791362 29.03694730466384 21.673018683315473 100-101 19.795029111159153 29.45706383581455 28.896459695395144 21.851447357631148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 770.0 1 463.5 2 151.5 3 140.0 4 105.5 5 99.5 6 176.0 7 313.0 8 482.5 9 580.5 10 500.0 11 363.5 12 341.0 13 379.0 14 423.5 15 484.5 16 535.0 17 577.0 18 615.5 19 672.0 20 757.5 21 848.5 22 944.5 23 1080.5 24 1196.5 25 1380.5 26 1727.5 27 2286.5 28 2889.5 29 3538.5 30 4626.5 31 5724.5 32 7037.5 33 8538.5 34 9671.5 35 10503.0 36 11530.5 37 12436.5 38 12152.0 39 11515.0 40 11025.5 41 10604.0 42 10429.5 43 10350.5 44 10176.5 45 9807.5 46 9480.5 47 9039.0 48 8586.0 49 8119.5 50 7453.5 51 6764.5 52 6125.5 53 5452.0 54 4704.5 55 4031.0 56 3561.5 57 3083.0 58 2557.5 59 2132.0 60 1735.5 61 1329.0 62 986.5 63 731.5 64 511.5 65 368.5 66 266.5 67 200.0 68 144.5 69 112.0 70 86.5 71 48.0 72 29.5 73 20.5 74 17.5 75 16.0 76 8.0 77 2.5 78 3.5 79 4.0 80 1.0 81 2.0 82 1.5 83 1.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020127624045801526 2 0.0 3 0.002236402671755725 4 0.01602755248091603 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.8636688931297708E-4 14-15 3.7273377862595417E-4 16-17 3.7273377862595417E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.8636688931297708E-4 28-29 0.005404639790076336 30-31 0.0 32-33 3.7273377862595417E-4 34-35 0.010809279580152672 36-37 0.003727337786259542 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.7273377862595417E-4 60-61 0.002609136450381679 62-63 0.009131977576335878 64-65 0.0024227695610687023 66-67 0.00447280534351145 68-69 0.0031682371183206107 70-71 1.8636688931297708E-4 72-73 0.006150107347328244 74-75 0.006150107347328244 76-77 0.002050035782442748 78-79 0.0014909351145038167 80-81 0.00969107824427481 82-83 0.011741114026717557 84-85 0.002609136450381679 86-87 0.002609136450381679 88-89 0.0013045682251908396 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.8636688931297708E-4 100-101 0.002236402671755725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.17190898470681 #Duplication Level Percentage of deduplicated Percentage of total 1 71.38590132087441 40.81268253108294 2 17.684757539429256 20.22142696921714 3 5.730590538038253 9.828864020080353 4 2.089843372203928 4.779213402717429 5 0.9728957402762565 2.7811153357341545 6 0.5137774980820946 1.7624184215243934 7 0.31191182411620855 1.2482816093738027 8 0.2128822978452375 0.9736709885490533 9 0.15102205184907289 0.7770797102698981 >10 0.8853820762492564 9.261352972282804 >50 0.04331568718686427 1.6655938625008415 >100 0.011157070942071102 1.0044592858559929 >500 0.003937789744260388 1.6340755285404744 >1k 0.002625193162840259 3.2497653622707343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2766 1.0309816316793892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2453 0.9143159589694657 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2353 0.8770425811068702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 0.4059070849236642 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 980 0.3652791030534351 RNA PCR Primer, Index 23 (95% over 23bp) TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 789 0.29408695133587787 RNA PCR Primer, Index 23 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 784 0.2922232824427481 RNA PCR Primer, Index 23 (96% over 25bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.24861343034351144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 615 0.22923127385496184 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.19382156488549618 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.1162929389312977 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 290 0.10809279580152671 RNA PCR Primer, Index 23 (96% over 28bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.863668893129771E-4 0.0 14-15 0.0 0.0 0.0 3.727337786259542E-4 0.0 16-17 0.0 0.0 0.0 3.727337786259542E-4 0.0 18-19 0.0 0.0 0.0 3.727337786259542E-4 0.0 20-21 0.0 0.0 0.0 3.727337786259542E-4 0.0 22-23 0.0 0.0 0.0 9.318344465648855E-4 0.0 24-25 0.0 0.0 0.0 0.002050035782442748 0.0 26-27 0.0 0.0 0.0 0.003540970896946565 0.0 28-29 0.0 0.0 0.0 0.006336474236641221 0.0 30-31 0.0 0.0 0.0 0.012300214694656489 0.0 32-33 0.0 0.0 0.0 0.021059458492366415 0.0 34-35 0.0 0.0 0.0 0.037087010973282444 0.0 36-37 0.0 0.0 0.0 0.05535096612595419 0.0 38-39 0.0 0.0 0.0 0.06914211593511452 0.0 40-41 0.0 0.0 0.0 0.07827409351145038 0.0 42-43 0.0 0.0 0.0 0.09336981154580153 0.0 44-45 0.0 0.0 0.0 0.11126103291984733 0.0 46-47 0.0 0.0 0.0 0.1293386211832061 0.0 48-49 0.0 0.0 0.0 0.15337994990458015 0.0 50-51 0.0 0.0 0.0 0.17257573950381677 0.0 52-53 0.0 0.0 0.0 0.1969898020038168 0.0 54-55 0.0 0.0 0.0 0.2415314885496183 0.0 56-57 0.0 0.0 0.0 0.2981870229007634 0.0 58-59 0.0 0.0 0.0 0.35689259303435117 0.0 60-61 0.0 0.0 0.0 0.40888895515267176 0.0 62-63 0.0 0.0 0.0 0.4815720419847328 0.0 64-65 0.0 0.0 0.0 0.5745691197519085 0.0 66-67 0.0 0.0 0.0 0.6936575620229007 0.0 68-69 0.0 0.0 0.0 0.8315690601145038 0.0 70-71 0.0 0.0 0.0 0.9679896230916031 0.0 72-73 0.0 0.0 0.0 1.115592199427481 0.0 74-75 0.0 0.0 0.0 1.2805268964694656 0.0 76-77 0.0 0.0 0.0 1.4599982108778626 0.0 78-79 0.0 0.0 0.0 1.6418922948473282 0.0 80-81 0.0 0.0 0.0 1.831427421278626 0.0 82-83 0.0 0.0 0.0 2.0405310710877864 0.0 84-85 0.0 0.0 0.0 2.2731169489503813 0.0 86-87 0.0 0.0 0.0 2.5435353053435117 0.0 88-89 0.0 0.0 0.0 2.8581226145038165 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1100 0.0 54.8394 1 TACTTCG 45 9.6131617E-4 42.22106 7 TGGTATC 430 0.0 41.97559 2 GTATCAA 1910 0.0 41.28159 1 GTGGTAT 455 0.0 40.71317 1 TATCAAC 2410 0.0 32.914036 2 ATCAACG 2455 0.0 32.310722 3 TCAACGC 2455 0.0 32.310722 4 CAACGCA 2480 0.0 31.98501 5 AACGCAG 2585 0.0 30.68581 6 TGTATCG 65 0.0058369758 29.229967 4 ACGCAGA 2895 0.0 27.235867 7 GACGCCA 35 0.008388776 27.14211 52-53 CGCAGAG 2935 0.0 26.86468 8 GCAGAGT 3110 0.0 25.200272 9 ACATCTC 1215 0.0 24.238016 8 GAGTACT 1850 0.0 24.134472 12-13 CTTATAC 1205 0.0 24.044983 1 TATACAC 1240 0.0 23.74935 3 ACACATC 1260 0.0 23.372374 6 >>END_MODULE