FastQCFastQC Report
Fri 10 Feb 2017
SRR2031691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443889
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.21919894388011418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.189011216768156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7740.17436791630340018No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.10836042343919314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.049.575461
GTATCAA11400.033.756971
ATCAACG11800.033.417923
TCAACGC11700.033.2974784
CAACGCA11900.032.7378545
AACGCAG12200.031.9256256
TATCAAC12750.030.555332
CGCCGTA1200.029.68693744-45
ACGCAGA14050.027.3838217
GTGGTAT3300.027.3541341
CGCAGAG14150.026.5189298
GCCGTAT1450.026.20639846-47
CGTATCA1400.025.44594648-49
GGATATA1452.3264436E-522.935771
TCGCCGT1354.0017767E-1122.86993644-45
TAAGGCC1252.2497194E-422.804714
CCGTATC1600.022.265246-47
GGTCGCC1507.2759576E-1222.16624542-43
GTCGCCG1406.548362E-1122.05315242-43
CTAAGAC1954.2009924E-721.9276073
Produced by FastQC (version 0.11.5)