##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031691_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 443889 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81602607859172 33.0 31.0 34.0 30.0 34.0 2 32.130165424238946 34.0 31.0 34.0 31.0 34.0 3 32.242121341146095 34.0 31.0 34.0 31.0 34.0 4 35.645458661962785 37.0 35.0 37.0 35.0 37.0 5 35.648790576022385 37.0 37.0 37.0 35.0 37.0 6 35.674276677277426 37.0 37.0 37.0 35.0 37.0 7 35.65442712029359 37.0 37.0 37.0 35.0 37.0 8 35.645413605653665 37.0 37.0 37.0 35.0 37.0 9 37.415597142528874 39.0 38.0 39.0 35.0 39.0 10-11 37.34709127732384 39.0 38.0 39.0 35.0 39.0 12-13 37.2950332177639 39.0 38.0 39.0 35.0 39.0 14-15 38.70087116373688 41.0 39.0 41.0 36.0 41.0 16-17 38.62884414797393 41.0 39.0 41.0 35.0 41.0 18-19 38.61618219870283 41.0 39.0 41.0 35.0 41.0 20-21 38.61356893277373 41.0 39.0 41.0 35.0 41.0 22-23 38.514521648430126 41.0 39.0 41.0 35.0 41.0 24-25 38.43680740004821 41.0 39.0 41.0 34.5 41.0 26-27 38.3275548616884 40.0 38.0 41.0 34.0 41.0 28-29 38.21404562852425 40.0 38.0 41.0 34.0 41.0 30-31 38.0572834650104 40.0 38.0 41.0 34.0 41.0 32-33 37.872448968097885 40.0 38.0 41.0 34.0 41.0 34-35 37.69773186539878 40.0 38.0 41.0 33.0 41.0 36-37 37.54279786162756 40.0 38.0 41.0 33.0 41.0 38-39 37.361447343817936 40.0 38.0 41.0 32.5 41.0 40-41 37.25677590568813 40.0 37.5 41.0 32.0 41.0 42-43 37.24256739860641 40.0 38.0 41.0 32.0 41.0 44-45 37.094964056329395 40.0 37.5 41.0 31.5 41.0 46-47 37.030611256417714 40.0 37.5 41.0 31.0 41.0 48-49 37.074421758592806 40.0 38.0 41.0 31.0 41.0 50-51 36.52375706539247 39.5 37.0 40.5 30.5 41.0 52-53 36.55399886007538 39.5 37.0 40.5 31.0 41.0 54-55 36.77424874236577 40.0 37.0 41.0 31.0 41.0 56-57 36.654217608456165 40.0 37.0 41.0 31.0 41.0 58-59 36.46704243628474 40.0 36.0 41.0 31.0 41.0 60-61 36.24354174129118 39.5 36.0 41.0 30.0 41.0 62-63 35.95564769570771 39.0 35.0 41.0 29.5 41.0 64-65 35.67902448585119 39.0 35.0 41.0 29.0 41.0 66-67 35.3796117948406 38.5 35.0 41.0 29.0 41.0 68-69 35.035301618197344 37.5 35.0 40.0 28.5 41.0 70-71 34.66614851911176 37.0 35.0 39.5 28.0 41.0 72-73 34.28855299410438 36.5 35.0 39.0 28.0 41.0 74-75 33.869675752271405 36.0 34.0 39.0 27.0 41.0 76-77 33.49083216750134 35.5 34.0 38.0 26.0 40.0 78-79 33.10705153765919 35.0 34.0 37.0 26.0 39.0 80-81 32.76439154833753 35.0 34.0 37.0 26.0 39.0 82-83 32.43386972869344 35.0 34.0 36.0 26.0 38.0 84-85 32.126191457774354 35.0 34.0 36.0 25.0 37.0 86-87 31.866805665380312 35.0 34.0 35.5 25.0 37.0 88-89 31.605338271504813 35.0 33.0 35.0 24.0 36.5 90-91 31.36794221077792 35.0 33.0 35.0 23.0 36.0 92-93 31.160017481847937 35.0 33.0 35.0 20.0 36.0 94-95 30.919413411911535 35.0 33.0 35.0 19.0 36.0 96-97 30.66538819389532 35.0 33.0 35.0 15.0 35.0 98-99 30.437099139649774 35.0 32.0 35.0 2.0 35.0 100-101 29.874419055214254 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02226505573344184 1101 2 0.0681123600367215 1101 3 0.25881473652369635 1101 4 0.27960323444732893 1101 5 0.12185124578672202 1101 6 0.2946352830090433 1101 7 0.47549849318171766 1101 8 0.44125901123749145 1101 9 0.5518328879896259 1101 10-11 0.4999524872735819 1101 12-13 0.14898428306990041 1101 14-15 0.3558329576021393 1101 16-17 0.5070265504116165 1101 18-19 0.6368391544532699 1101 20-21 0.5223048018833225 1101 22-23 0.5771854526761118 1101 24-25 0.418517509681287 1101 26-27 0.04569916164211918 1101 28-29 0.18077724897657532 1101 30-31 0.2843810937196096 1101 32-33 0.25271738348317285 1101 34-35 0.40023142434849035 1101 36-37 0.05390161539926197 1101 38-39 0.18431643378423246 1101 40-41 0.20007132044427323 1101 42-43 0.10262560800732246 1101 44-45 -0.03476557448287565 1101 46-47 0.0360576591167856 1101 48-49 0.13184153265372345 1101 50-51 0.3132630090171986 1101 52-53 0.07628846117852106 1101 54-55 0.0995904747918459 1101 56-57 -0.14538824705068265 1101 58-59 -0.3932982077301901 1101 60-61 -0.6673660585647525 1101 62-63 -0.3401226313992254 1101 64-65 -0.09614088824650935 1101 66-67 -0.2865715924019838 1101 68-69 -0.3618757286814116 1101 70-71 -0.37137241229873297 1101 72-73 -0.1996299437941289 1101 74-75 0.16430722775845652 1101 76-77 0.02083183477824946 1101 78-79 -0.3917499120405097 1101 80-81 -0.5079951247746095 1101 82-83 -0.6288703100188577 1101 84-85 -0.4667559850100744 1101 86-87 -0.6610892719162145 1101 88-89 -0.8139232631294924 1101 90-91 -0.936802213368761 1101 92-93 -0.7562347149873361 1101 94-95 -0.897053464319491 1101 96-97 -0.9108525061116239 1101 98-99 -1.1445764559071954 1101 100-101 -1.00782695791521 1102 1 -0.14391741914093004 1102 2 -0.279144758891249 1102 3 -0.04667102562035552 1102 4 0.039862865938111725 1102 5 -0.011798775151298457 1102 6 -0.3031714590345089 1102 7 -0.30199574886183456 1102 8 -0.30480202310086923 1102 9 -0.04401091435543236 1102 10-11 -0.14402913961084352 1102 12-13 -9.450511009490015E-4 1102 14-15 0.14488237133748072 1102 16-17 -0.03501961793008945 1102 18-19 -0.07049962276616384 1102 20-21 -0.4073826981166775 1102 22-23 -0.07689222739088564 1102 24-25 -0.41836220137398783 1102 26-27 -0.23124545728585133 1102 28-29 -0.1650600102361537 1102 30-31 -0.1794288413725127 1102 32-33 -0.10960856919690087 1102 34-35 0.25392044235518085 1102 36-37 0.13461115266894552 1102 38-39 -0.011100756333014772 1102 40-41 -0.17375091573662615 1102 42-43 0.0990988596823641 1102 44-45 0.1746146600229963 1102 46-47 0.09123589404141086 1102 48-49 0.20079960426176058 1102 50-51 0.32323474268553554 1102 52-53 0.17956526184854482 1102 54-55 0.17070778006822707 1102 56-57 0.1719013258136428 1102 58-59 -0.05052313444711132 1102 60-61 0.024243028537419775 1102 62-63 0.11356507798100068 1102 64-65 -0.09334042760999495 1102 66-67 0.04923504495145181 1102 68-69 0.0732163986217671 1102 70-71 0.04109356341316328 1102 72-73 0.21043470235327533 1102 74-75 -0.18841340666365625 1102 76-77 0.3984229151802623 1102 78-79 0.3277132893832686 1102 80-81 0.2505240335168466 1102 82-83 0.5248016958437844 1102 84-85 0.29421684790450087 1102 86-87 0.28263324692800396 1102 88-89 0.1313434349442062 1102 90-91 0.07494270916055612 1102 92-93 -0.030758374953833822 1102 94-95 -0.09952415108665846 1102 96-97 -0.2576777783059292 1102 98-99 0.034037970390965455 1102 100-101 0.11657761704291048 1103 1 -0.3188807775998903 1103 2 -0.19490847329661065 1103 3 -0.12920609680963224 1103 4 -0.17091759888600677 1103 5 -0.05783625421327798 1103 6 -0.3355730503242853 1103 7 -0.5245015068182823 1103 8 -0.17853265542917995 1103 9 -0.48202127867703837 1103 10-11 -0.2565579293930824 1103 12-13 -0.08148446693009959 1103 14-15 -0.10250037573120352 1103 16-17 -0.020317199588383517 1103 18-19 -0.008994178880065817 1103 20-21 -0.1677993647833489 1103 22-23 -0.36942913065721683 1103 24-25 -0.22080540698538442 1103 26-27 -0.15872792169121652 1103 28-29 -0.2723477510234247 1103 30-31 -0.33280640628038327 1103 32-33 -0.38269928318349855 1103 34-35 -0.5190394089848525 1103 36-37 -0.4005254679340666 1103 38-39 -0.47063148288242473 1103 40-41 -0.22831409622239818 1103 42-43 0.03491727467397965 1103 44-45 -0.47356765781620425 1103 46-47 -0.4405048408832144 1103 48-49 -0.16373138401294796 1103 50-51 -0.3221536576494728 1103 52-53 -0.32345112215481464 1103 54-55 -0.4915553585414898 1103 56-57 -0.40189866371735405 1103 58-59 -0.2331419577301972 1103 60-61 -0.1621577252314239 1103 62-63 -0.3010601313992254 1103 64-65 -0.42426588824651645 1103 66-67 -0.8113111757353124 1103 68-69 -0.794167395348083 1103 70-71 -0.7125182456320616 1103 72-73 -0.680098693794136 1103 74-75 -0.5752761055748792 1103 76-77 -0.7265639985550791 1103 78-79 -0.6430519953738525 1103 80-81 -0.4637242914412738 1103 82-83 -0.8658494766855256 1103 84-85 -0.9667559850100744 1103 86-87 -0.7248913552495466 1103 88-89 -0.8191315964628281 1103 90-91 -0.7271667967020896 1103 92-93 -1.3252451316540004 1103 94-95 -0.7863763809861588 1103 96-97 -1.115279589444956 1103 98-99 -1.454472289240524 1103 100-101 -1.3841290412485456 1104 1 0.14291210561178858 1104 2 0.12605031624110552 1104 3 0.25242787520983256 1104 4 0.18258376972713108 1104 5 0.10096088690594485 1104 6 0.2268509156124523 1104 7 0.12097066471456941 1104 8 0.23511165722288752 1104 9 0.19105125781931065 1104 10-11 0.2670316387334353 1104 12-13 0.27339501907963637 1104 14-15 0.30641081648291646 1104 16-17 0.4321520066160005 1104 18-19 0.45869134423428193 1104 20-21 0.4204761826619148 1104 22-23 0.4944223748172405 1104 24-25 0.37531115323360353 1104 26-27 0.2074046056810488 1104 28-29 0.299152781215021 1104 30-31 0.3785587853254526 1104 32-33 0.5282553244807389 1104 34-35 0.33249457824143036 1104 36-37 0.38085759167663724 1104 38-39 0.04329225495212086 1104 40-41 0.5040174883274844 1104 42-43 0.6258445100754457 1104 44-45 0.4621930629867137 1104 46-47 0.5348316098467123 1104 48-49 0.6090788349651604 1104 50-51 0.6302870357811869 1104 52-53 0.7340591424437335 1104 54-55 0.696866554475541 1104 56-57 0.7234149767935989 1104 58-59 0.7695382429997366 1104 60-61 0.894943856277095 1104 62-63 0.791929527968172 1104 64-65 0.41074019447854937 1104 66-67 0.4516241192768504 1104 68-69 0.31817293311907235 1104 70-71 0.33941301957474934 1104 72-73 0.4123017015829973 1104 74-75 0.42771773566600046 1104 76-77 0.2460827471870104 1104 78-79 -0.03793490291642598 1104 80-81 -0.009116627207703232 1104 82-83 -0.03487319931326027 1104 84-85 -0.11423355496141596 1104 86-87 0.00414225180641381 1104 88-89 0.0662185403984843 1104 90-91 0.183151025682335 1104 92-93 0.15955755995183907 1104 94-95 0.3155377765564218 1104 96-97 0.28788152673508804 1104 98-99 0.13520988837504433 1104 100-101 0.24866445023564054 1105 1 -0.23272732231533766 1105 2 0.10479935190664236 1105 3 -0.051144613069794786 1105 4 -0.03970570864210288 1105 5 0.05407939619323088 1105 6 0.04618507975701647 1105 7 0.010775424076030049 1105 8 0.1056772022944017 1105 9 0.15605036766442737 1105 10-11 0.1332413592248045 1105 12-13 0.013885197704048835 1105 14-15 0.019958973862294727 1105 16-17 -0.01389570568594678 1105 18-19 0.032215170713428165 1105 20-21 0.06212949700527304 1105 22-23 -0.12741312455965925 1105 24-25 -0.15431023422114976 1105 26-27 -0.0021793952684490137 1105 28-29 -0.15558580996650306 1105 30-31 0.11512931526433334 1105 32-33 0.26223847088154884 1105 34-35 0.06921643451109105 1105 36-37 0.199055324748862 1105 38-39 0.20292669801187913 1105 40-41 0.2717938814198817 1105 42-43 0.29748714256016484 1105 44-45 0.3319010921837986 1105 46-47 0.4240340310680111 1105 48-49 0.3279352826537263 1105 50-51 0.2941319114562262 1105 52-53 0.4847742335362497 1105 54-55 0.14435038332842964 1105 56-57 0.31581602124200714 1105 58-59 0.37500082682264946 1105 60-61 0.3270191040368644 1105 62-63 0.4194175108771958 1105 64-65 0.5122178515908828 1105 66-67 0.3963425336142805 1105 68-69 0.7056039461153318 1105 70-71 0.7955610226606211 1105 72-73 0.4347514078318824 1105 74-75 0.5711098192218742 1105 76-77 0.466182444534347 1105 78-79 0.4878677200733108 1105 80-81 0.445167984981488 1105 82-83 0.4612338566478158 1105 84-85 0.4418758747460245 1105 86-87 0.4574892341813488 1105 88-89 0.5032578852444907 1105 90-91 0.32113096752555137 1105 92-93 0.3103557321671353 1105 94-95 0.1820115763309147 1105 96-97 0.2511774735631711 1105 98-99 0.44275840181638415 1105 100-101 0.405124007531942 1106 1 0.2646013652572492 1106 2 0.23418378860814926 1106 3 0.43143378414274736 1106 4 0.4355556153997071 1106 5 0.305705412453392 1106 6 0.5236531401519002 1106 7 0.37244789794362276 1106 8 0.42228579695177615 1106 9 0.3580828879896316 1106 10-11 0.46747480870215696 1106 12-13 0.4236122592603735 1106 14-15 0.2564281956973744 1106 16-17 0.2390950027925669 1106 18-19 0.22820820207230952 1106 20-21 0.4795220637880817 1106 22-23 0.37554854791421377 1106 24-25 0.48555620015748246 1106 26-27 0.4916068997373557 1106 28-29 0.630070403738479 1106 30-31 0.6751177008624722 1106 32-33 0.5927843477688839 1106 34-35 0.5229620791103855 1106 36-37 0.2566917939706954 1106 38-39 0.5074563147366149 1106 40-41 0.4180772728252222 1106 42-43 0.5154604294358904 1106 44-45 0.43547252075522636 1106 46-47 0.783267480545355 1106 48-49 0.6196019493203906 1106 50-51 0.43960229473148615 1106 52-53 0.45872893736899556 1106 54-55 0.3713538676489918 1106 56-57 0.4247010386636063 1106 58-59 0.5584503041745634 1106 60-61 0.38456846524476873 1106 62-63 0.45280891621981567 1106 64-65 0.5191864927058703 1106 66-67 0.3289790028361139 1106 68-69 0.4369337951281125 1106 70-71 0.6294460400822217 1106 72-73 0.4567316633487266 1106 74-75 0.5441063349013149 1106 76-77 0.48854016811158374 1106 78-79 0.6401325284356787 1106 80-81 0.6401447561777687 1106 82-83 0.7005195709335226 1106 84-85 0.8332812173708746 1106 86-87 0.752117573321879 1106 88-89 0.8372300106800274 1106 90-91 0.5447082032979047 1106 92-93 0.7215926659650442 1106 94-95 0.7184227261566996 1106 96-97 1.1151147557931367 1106 98-99 1.17127175837852 1106 100-101 1.0311983397038382 1107 1 0.10844543138111007 1107 2 0.29490424259285675 1107 3 0.2793242356601411 1107 4 0.31867922753886546 1107 5 0.2601959133169487 1107 6 0.24025812411439773 1107 7 0.28547528506427966 1107 8 0.32655424008031986 1107 9 0.2927115581105255 1107 10-11 0.3516161854601094 1107 12-13 0.32397618721497423 1107 14-15 0.45632950337070355 1107 16-17 0.31430472398674425 1107 18-19 0.29319709918556214 1107 20-21 0.3193012397071584 1107 22-23 0.4055263421407105 1107 24-25 0.47344788532895876 1107 26-27 0.6303683110376355 1107 28-29 0.4919751656432467 1107 30-31 0.5258332202308438 1107 32-33 0.3905020877146015 1107 34-35 0.38016045068700066 1107 36-37 0.5272999854338067 1107 38-39 0.48047629993277496 1107 40-41 0.3465253359883107 1107 42-43 0.5619506835686323 1107 44-45 0.6940772579869048 1107 46-47 0.6017352821737774 1107 48-49 0.5243083396485417 1107 50-51 0.45906935616745415 1107 52-53 0.28154536964139254 1107 54-55 0.48238273515453756 1107 56-57 0.39598157160216374 1107 58-59 0.5130394973647938 1107 60-61 0.470776212592412 1107 62-63 0.4998358098788387 1107 64-65 0.5587867887828466 1107 66-67 0.36836957771891576 1107 68-69 0.4455508689006251 1107 70-71 0.33298045903114826 1107 72-73 0.4592174763267636 1107 74-75 0.4854090736133827 1107 76-77 0.5803363684570044 1107 78-79 0.6200363034171659 1107 80-81 0.6512908208212451 1107 82-83 0.6363633903265651 1107 84-85 0.5713147727619443 1107 86-87 0.5774753330060598 1107 88-89 0.6002944074231813 1107 90-91 0.5712194295500659 1107 92-93 0.7001825993477233 1107 94-95 0.9174974855941507 1107 96-97 1.0321391821439896 1107 98-99 1.081770154628213 1107 100-101 1.2815931090105188 1108 1 0.20770913468081176 1108 2 0.438488027466164 1108 3 0.5569650034954776 1108 4 0.5363728759423694 1108 5 0.48026681596216036 1108 6 0.5195232502096587 1108 7 0.45433740431825953 1108 8 0.4931695280185693 1108 9 0.527686244206258 1108 10-11 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80-81 -0.1788885197832144 2316 82-83 -0.18919643595115332 2316 84-85 -0.05971935635258774 2316 86-87 -0.05142516907628547 2316 88-89 -0.1870384919017276 2316 90-91 -0.09258382194592585 2316 92-93 -0.06872575055819041 2316 94-95 -0.04112976954037606 2316 96-97 -0.17980779153905146 2316 98-99 0.14358960117829156 2316 100-101 -0.08047693281481116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5088.0 3 492.0 4 390.0 5 357.0 6 377.0 7 423.0 8 474.0 9 578.0 10 641.0 11 713.0 12 908.0 13 1126.0 14 1305.0 15 1468.0 16 1712.0 17 2277.0 18 2115.0 19 1471.0 20 1432.0 21 1502.0 22 1483.0 23 1547.0 24 1772.0 25 2000.0 26 2395.0 27 2889.0 28 3409.0 29 4307.0 30 5272.0 31 6750.0 32 8534.0 33 11819.0 34 16651.0 35 26955.0 36 46294.0 37 94039.0 38 137344.0 39 45501.0 40 79.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.29416396490302 19.709224802528716 13.56206385412507 24.4345473784432 2 16.283283281024723 21.68464563642394 38.679566699683896 23.352504382867444 3 18.710009295441623 25.44920447264162 30.69797034483381 25.142815887082946 4 13.486895543401744 18.55110918575477 37.378386671720726 30.58360859912276 5 14.188965115833122 36.655785339310164 34.85596717102785 14.299282373828866 6 30.350452586158283 36.582224517921865 18.661256233914752 14.406066662005093 7 25.78731409777786 32.623598331645276 23.399599829465092 18.18948774111177 8 24.177792213357876 34.359161743908295 21.939826186593407 19.52321985614042 9 25.25638220852946 16.607612865684366 21.726460989532704 36.40954393625347 10-11 23.585086494812117 26.417845150494884 29.635438630515722 20.36162972417728 12-13 24.695639425055965 24.897851008520405 28.696902542587928 21.709607023835705 14-15 22.23125878432797 26.740001940166174 26.585352647762832 24.44338662774302 16-17 21.724867307052325 28.940204976056044 27.555009053015134 21.779918663876497 18-19 21.515764442042872 28.109880118268567 29.155164886734497 21.21919055295406 20-21 22.38909004771412 27.433363789155475 28.860726653356345 21.316819509774064 22-23 22.22060555202144 27.496401968878725 28.455124994924407 21.82786748417543 24-25 22.01733572114886 27.544421939633978 28.83772177137418 21.60052056784298 26-27 21.83422316355508 27.717252781706264 28.90470809283106 21.543815961907598 28-29 21.834045375186655 27.664292191299406 29.036984070413197 21.46467836310074 30-31 22.142054046982963 27.451911311794685 28.798856945667705 21.60717769555465 32-33 21.99882040230371 27.6561529101442 28.689597775229124 21.65542891232296 34-35 22.079669573612808 27.749680807388128 28.686505483796743 21.48414413520232 36-37 21.956482261632047 27.615074087985192 28.711307821845942 21.71713582853682 38-39 22.020225487000722 27.666197137034608 28.707583035131268 21.6059943408334 40-41 22.202773936992067 27.647115876943477 28.632249216742867 21.517860969321585 42-43 22.20787510262263 27.679014461896557 28.375043981126456 21.738066454354357 44-45 22.148555624610157 27.83988305584287 28.209055727816086 21.802505591730892 46-47 22.081683754610363 27.71867492301966 28.196911763047183 22.0027295593228 48-49 22.053692592479788 27.677889566842754 28.39837065463722 21.870047186040242 50-51 22.09422985389094 27.983482931384913 28.028373625927422 21.893913588796725 52-53 22.236055664569097 28.01260797979114 27.81830074204389 21.93303561359587 54-55 22.20203721934107 27.943599267927162 27.821587127606428 22.032776385125345 56-57 22.020382935720214 28.066463348612263 27.938900819287543 21.974252896379976 58-59 22.015418551883112 28.103333167930494 28.078522742138478 21.802725538047916 60-61 22.192099060593417 28.125472240704614 27.98292603159925 21.699502667102728 62-63 22.094810621225534 28.09730296656761 28.017908798204612 21.789977614002247 64-65 22.081585875653957 28.042155560017417 27.93849249768196 21.937766066646663 66-67 22.077738978218107 28.258147907530724 27.708637864760977 21.955475249490192 68-69 21.91899333272424 28.1447918887306 27.933302427673425 22.002912350871732 70-71 22.014092989369356 27.95635864943689 27.961320969637367 22.068227391556388 72-73 22.043788705342592 28.000590977221435 27.90562860690654 22.049991710529433 74-75 22.02485456774003 28.03778363378246 27.81181251613878 22.12554928233873 76-77 21.974881145067872 28.013106470332794 27.960996407563854 22.05101597703548 78-79 21.90018791183258 27.96446142844575 27.82459908006668 22.31075157965499 80-81 21.937995163415206 27.99703578711808 27.878828211005036 22.186140838461675 82-83 21.939521368755415 28.105824068726537 27.755468524400804 22.19918603811724 84-85 22.033114524824615 28.089598700683492 27.57483476573955 22.30245200875234 86-87 21.914419622462734 28.144968895757078 27.680463828275254 22.260147653504937 88-89 22.057808475027407 28.063885267275097 27.59831365631612 22.279992601381377 90-91 21.919469234170418 27.883140379530957 27.90615045884805 22.291239927450572 92-93 22.031802757923614 27.79774474767165 27.789509782687173 22.380942711717566 94-95 21.973753659735912 27.83936860814999 27.905345357266913 22.28153237484718 96-97 21.81590396009012 27.848957710775167 27.86440655030796 22.470731778826742 98-99 21.895999359482534 27.808494299056253 27.870741771098455 22.42476457036276 100-101 21.92838665206075 27.8471658925608 27.772368876769942 22.452078578608507 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1366.0 1 780.0 2 175.5 3 153.0 4 108.0 5 92.0 6 137.0 7 225.0 8 335.5 9 360.5 10 281.0 11 202.5 12 192.5 13 212.0 14 235.0 15 255.5 16 298.5 17 333.5 18 353.5 19 386.0 20 438.0 21 538.5 22 656.0 23 802.0 24 989.5 25 1349.0 26 1909.5 27 2580.0 28 3469.0 29 4697.0 30 5989.0 31 7319.5 32 8536.5 33 9715.5 34 10875.0 35 11841.5 36 12879.5 37 13760.0 38 14211.5 39 14598.5 40 15061.0 41 15556.0 42 15952.0 43 16339.5 44 16657.0 45 17106.0 46 17311.0 47 17144.0 48 16950.0 49 16565.5 50 16146.0 51 15417.5 52 14591.5 53 13873.5 54 13159.5 55 12067.0 56 10656.0 57 9277.0 58 8166.5 59 7040.5 60 5780.5 61 4654.5 62 3643.5 63 2805.5 64 2131.0 65 1625.5 66 1183.0 67 871.5 68 688.0 69 512.5 70 405.0 71 323.0 72 255.5 73 187.5 74 153.5 75 129.0 76 114.5 77 107.0 78 84.5 79 57.5 80 40.5 81 40.0 82 27.5 83 16.5 84 15.0 85 13.5 86 8.5 87 5.5 88 4.0 89 2.0 90 2.5 91 2.0 92 1.0 93 1.5 94 1.0 95 0.0 96 1.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14868582010367457 2 0.15454314028957689 3 0.1489111016492862 4 0.15341673256151875 5 0.1401251213704327 6 0.12345428699517222 7 0.13021273336352104 8 0.1216520346302792 9 0.11444302517070709 10-11 0.10182725861645592 12-13 0.12198995694869663 14-15 0.14147681064410247 16-17 0.15015015015015015 18-19 0.14879846087648038 20-21 0.14057568446165597 22-23 0.13314139345647222 24-25 0.11872337453732802 26-27 0.12300372390394897 28-29 0.12683351017934663 30-31 0.11613263676279431 32-33 0.11658319985401756 34-35 0.1313391410915792 36-37 0.13483100504855944 38-39 0.12108883076625011 40-41 0.12345428699517222 42-43 0.11647055908121173 44-45 0.13528156813978268 46-47 0.13381723809330712 48-49 0.1449686746010827 50-51 0.13291611191086058 52-53 0.11692112217243501 54-55 0.1105005981225036 56-57 0.12998745181790944 58-59 0.11872337453732802 60-61 0.11804752990049314 62-63 0.11973714149258036 64-65 0.1410262475528792 66-67 0.13178970418280245 68-69 0.1361826943222292 70-71 0.12345428699517222 72-73 0.1251438985872594 74-75 0.1057696856646594 76-77 0.13438044195733617 78-79 0.13426780118453038 80-81 0.13539420891258852 82-83 0.14080096600726758 84-85 0.1304380149091327 86-87 0.1400124805976269 88-89 0.12683351017934663 90-91 0.13629533509503503 92-93 0.1480099754668397 94-95 0.1241301316320071 96-97 0.1103879573496978 98-99 0.11207756894178499 100-101 0.1553316256992176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 443889.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.15196713986457 #Duplication Level Percentage of deduplicated Percentage of total 1 76.02207216361833 54.85142052653802 2 16.73367116596657 24.14734584192238 3 4.651027968281509 10.067424514021155 4 1.4457434553946036 4.172529371452228 5 0.5314525441579065 1.917267325123935 6 0.236398832889751 1.0233984493538189 7 0.12554482267133893 0.63408141439738 8 0.06967933521125126 0.40220008835918475 9 0.04353875565422433 0.2827266180546768 >10 0.13554785662618848 1.4478927749896806 >50 0.0028181116118954045 0.1341993135908153 >100 0.0015656174478136128 0.3352723398279954 >500 9.393704686881677E-4 0.5842414223687307 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 973 0.21919894388011418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.189011216768156 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 774 0.17436791630340018 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 481 0.10836042343919314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.505630912232563E-4 0.0 10-11 0.0 0.0 0.0 4.505630912232563E-4 0.0 12-13 0.0 0.0 0.0 4.505630912232563E-4 0.0 14-15 0.0 0.0 0.0 4.505630912232563E-4 0.0 16-17 0.0 0.0 0.0 4.505630912232563E-4 0.0 18-19 0.0 0.0 0.0 4.505630912232563E-4 0.0 20-21 0.0 0.0 0.0 5.632038640290704E-4 0.0 22-23 0.0 0.0 0.0 0.001576970819281397 0.0 24-25 0.0 0.0 0.0 0.002027533910504653 0.0 26-27 0.0 0.0 0.0 0.0025907377745337237 0.0 28-29 0.0 0.0 0.0 0.004618271685038377 0.0 30-31 0.0 0.0 0.0 0.00833541718763024 0.0 32-33 0.0 0.0 0.0 0.013291611191086058 0.0 34-35 0.0 0.0 0.0 0.018698368285765134 0.0 36-37 0.0 0.0 0.0 0.025794736972531422 0.0 38-39 0.0 0.0 0.0 0.03435543570577329 0.0 40-41 0.0 0.0 0.0 0.04088860052851051 0.0 42-43 0.0 0.0 0.0 0.05035042544419889 0.0 44-45 0.0 0.0 0.0 0.055306619447654706 0.0 46-47 0.0 0.0 0.0 0.06307883277125588 0.0 48-49 0.0 0.0 0.0 0.07783477400881753 0.0 50-51 0.0 0.0 0.0 0.09405504529285474 0.0 52-53 0.0 0.0 0.0 0.11635791830840593 0.0 54-55 0.0 0.0 0.0 0.13674589818625826 0.0 56-57 0.0 0.0 0.0 0.1712139746648374 0.0 58-59 0.0 0.0 0.0 0.2074843035083095 0.0 60-61 0.0 0.0 0.0 0.23744674907465604 0.0 62-63 0.0 0.0 0.0 0.25839793281653745 0.0 64-65 0.0 0.0 0.0 0.3140424745826096 0.0 66-67 0.0 0.0 0.0 0.39435534559315505 0.0 68-69 0.0 0.0 0.0 0.48525644924744704 0.0 70-71 0.0 0.0 0.0 0.5824654361788646 0.0 72-73 0.0 0.0 0.0 0.68215252011201 0.0 74-75 0.0 0.0 0.0 0.7924278366889019 0.0 76-77 0.0 0.0 0.0 0.9225279292796172 0.0 78-79 0.0 0.0 0.0 1.0609634390579628 0.0 80-81 0.0 0.0 0.0 1.2058194728862395 0.0 82-83 0.0 0.0 0.0 1.3596867685389815 0.0 84-85 0.0 0.0 0.0 1.5330409178871296 0.0 86-87 0.0 0.0 0.0 1.7397367359857983 0.0 88-89 0.0 0.0 0.0 1.9920520670708217 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 690 0.0 49.57546 1 GTATCAA 1140 0.0 33.75697 1 ATCAACG 1180 0.0 33.41792 3 TCAACGC 1170 0.0 33.297478 4 CAACGCA 1190 0.0 32.737854 5 AACGCAG 1220 0.0 31.925625 6 TATCAAC 1275 0.0 30.55533 2 CGCCGTA 120 0.0 29.686937 44-45 ACGCAGA 1405 0.0 27.383821 7 GTGGTAT 330 0.0 27.354134 1 CGCAGAG 1415 0.0 26.518929 8 GCCGTAT 145 0.0 26.206398 46-47 CGTATCA 140 0.0 25.445946 48-49 GGATATA 145 2.3264436E-5 22.93577 1 TCGCCGT 135 4.0017767E-11 22.869936 44-45 TAAGGCC 125 2.2497194E-4 22.80471 4 CCGTATC 160 0.0 22.2652 46-47 GGTCGCC 150 7.2759576E-12 22.166245 42-43 GTCGCCG 140 6.548362E-11 22.053152 42-43 CTAAGAC 195 4.2009924E-7 21.927607 3 >>END_MODULE