FastQCFastQC Report
Fri 10 Feb 2017
SRR2031691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443889
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT22510.507108759171775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.28881094147410724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.25974962209020724No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT9490.2137921867854351RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6660.15003750937734434RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.1410262475528792No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.1351689273669769No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.13471836427575362No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG4720.10633288952868848RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.056.5241131
GTATCAA11750.036.7883722
TCAACGC12950.033.375655
ATCAACG13350.032.3792764
CAACGCA13400.032.254826
CCTAGCG300.003952054331.66407484-85
AACGCAG14400.030.6745747
TATCAAC14150.030.5486453
CGCAGAG15850.027.269069
ACGCAGA16350.027.0161368
GTATAGG908.919625E-426.3986241
TCGACTG458.9502113E-426.3867312-13
CCTAGAC1252.2524036E-422.8007053
GTGGTAT3450.022.0371131
CAGAGTA17150.021.74020410-11
GAGTACT12400.021.2583412-13
ACTAACC1456.136614E-419.6557793
GCAGAGT18650.019.3549869
GTACTTT13700.019.06778114-15
TGGTATC3759.094947E-1219.0005862
Produced by FastQC (version 0.11.5)