##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031691_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 443889 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77123334887776 34.0 31.0 34.0 31.0 34.0 2 32.941694883180254 34.0 31.0 34.0 31.0 34.0 3 32.996850563992346 34.0 31.0 34.0 31.0 34.0 4 36.379946337935834 37.0 37.0 37.0 35.0 37.0 5 36.34891605784329 37.0 37.0 37.0 35.0 37.0 6 36.38123720119219 37.0 37.0 37.0 35.0 37.0 7 36.33002620024376 37.0 37.0 37.0 35.0 37.0 8 36.31172883310918 37.0 37.0 37.0 35.0 37.0 9 38.163955403265234 39.0 39.0 39.0 37.0 39.0 10-11 38.13811110435266 39.0 39.0 39.0 37.0 39.0 12-13 38.08905717420346 39.0 39.0 39.0 37.0 39.0 14-15 39.58901774993298 41.0 40.0 41.0 37.0 41.0 16-17 39.56522914512412 41.0 40.0 41.0 37.0 41.0 18-19 39.565273075025516 41.0 39.5 41.0 37.0 41.0 20-21 39.54681350517809 41.0 40.0 41.0 37.0 41.0 22-23 39.46555557808371 41.0 39.0 41.0 36.0 41.0 24-25 39.40314357868746 41.0 39.0 41.0 36.0 41.0 26-27 39.274356877507664 41.0 39.0 41.0 36.0 41.0 28-29 39.176901207283805 41.0 39.0 41.0 36.0 41.0 30-31 39.014094739901196 40.0 39.0 41.0 35.0 41.0 32-33 38.81176375174874 40.0 38.0 41.0 35.0 41.0 34-35 38.6651144768174 40.0 38.0 41.0 35.0 41.0 36-37 38.56449134806224 40.0 38.0 41.0 35.0 41.0 38-39 38.60892475371095 40.0 38.0 41.0 35.0 41.0 40-41 38.53273228216964 40.0 38.0 41.0 35.0 41.0 42-43 38.43225558641913 40.0 38.0 41.0 35.0 41.0 44-45 38.25612258920586 40.0 38.0 41.0 34.0 41.0 46-47 38.05103753415831 40.0 38.0 41.0 34.0 41.0 48-49 37.82062970697629 40.0 38.0 41.0 33.0 41.0 50-51 37.662193701578545 40.0 38.0 41.0 33.0 41.0 52-53 37.72281358627945 40.0 38.0 41.0 33.0 41.0 54-55 37.65037768451121 40.0 37.5 41.0 33.0 41.0 56-57 37.46607823126953 40.0 37.0 41.0 33.0 41.0 58-59 37.26203510337044 40.0 37.0 41.0 33.0 41.0 60-61 36.98461777606563 40.0 36.0 41.0 32.5 41.0 62-63 36.68855276882283 39.0 36.0 41.0 32.0 41.0 64-65 36.33634084196725 39.0 35.0 41.0 31.0 41.0 66-67 35.93898136696336 38.5 35.0 40.5 31.0 41.0 68-69 35.49535582093722 37.5 35.0 40.0 30.0 41.0 70-71 35.135859415304274 37.0 35.0 39.5 30.0 41.0 72-73 34.748011327156114 36.5 35.0 39.0 29.5 41.0 74-75 34.36801993291115 36.0 35.0 39.0 29.0 40.5 76-77 33.44012805003052 35.0 33.5 37.0 28.5 39.0 78-79 33.63035015510634 35.0 34.0 37.0 29.0 39.0 80-81 33.39141992705383 35.0 34.0 37.0 29.0 39.0 82-83 33.09972876101908 35.0 34.0 36.5 29.0 38.5 84-85 32.80526888478877 35.0 34.0 36.0 29.0 37.0 86-87 32.54900211539371 35.0 34.0 36.0 28.0 37.0 88-89 32.3106339647975 35.0 34.0 35.0 27.0 36.5 90-91 32.105140023744674 35.0 34.0 35.0 27.0 36.0 92-93 31.93827060368696 35.0 34.0 35.0 26.5 36.0 94-95 31.76026776964511 35.0 34.0 35.0 26.0 36.0 96-97 31.59240485797125 35.0 33.5 35.0 25.0 35.5 98-99 31.391281378903287 35.0 33.0 35.0 25.0 35.0 100-101 30.70159206468284 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18919054353062847 1101 2 0.2232808593261808 1101 3 0.22694392207537817 1101 4 0.17467723696250204 1101 5 0.0992744008728863 1101 6 0.05495653536301859 1101 7 0.17775385503643548 1101 8 0.07491694526416381 1101 9 0.24541364872915494 1101 10-11 0.07481660583331262 1101 12-13 0.2307243431546553 1101 14-15 0.07611449051078978 1101 16-17 0.2227308623579134 1101 18-19 0.0032354609013012237 1101 20-21 0.13188215916140678 1101 22-23 0.18125835352580566 1101 24-25 0.1466293095808595 1101 26-27 0.214671364739921 1101 28-29 0.27218823109431156 1101 30-31 0.26749563434081125 1101 32-33 0.41799010315761365 1101 34-35 0.05240123818021658 1101 36-37 0.22855293131782872 1101 38-39 0.5359843983749784 1101 40-41 0.21972720611574204 1101 42-43 0.29513326243295523 1101 44-45 0.15841546143362706 1101 46-47 0.31389279175041906 1101 48-49 -0.1940886035698952 1101 50-51 -0.19408382070783858 1101 52-53 -0.22764172741422328 1101 54-55 -0.24411113891603264 1101 56-57 -0.37464710101125576 1101 58-59 -0.4502922483507277 1101 60-61 -0.650151371052111 1101 62-63 -0.389493557577147 1101 64-65 -0.15020179072993045 1101 66-67 -0.6940467319916266 1101 68-69 -0.4855785193071398 1101 70-71 -0.81787789015263 1101 72-73 -0.593161431147891 1101 74-75 -0.6064266028895702 1101 76-77 -0.7086070139840785 1101 78-79 -0.781518499202349 1101 80-81 -0.676936694025045 1101 82-83 -0.7256497228516707 1101 84-85 -0.7992318030082473 1101 86-87 -0.5536724588726827 1101 88-89 -0.5689488931987441 1101 90-91 -0.7410464703800344 1101 92-93 -0.5911569178700944 1101 94-95 -0.5247044243621097 1101 96-97 -0.43427800263010496 1101 98-99 -0.694601087808902 1101 100-101 -0.6606915439448819 1102 1 0.1645229346529078 1102 2 0.14893778981193861 1102 3 0.17598600750251592 1102 4 0.1093898625906462 1102 5 0.16364573671878446 1102 6 0.19758373385548111 1102 7 0.1919131933948961 1102 8 0.12855754409163467 1102 9 0.1094211864175989 1102 10-11 0.13454886085843754 1102 12-13 0.14985777070908313 1102 14-15 0.17401937953926705 1102 16-17 0.16822657006311204 1102 18-19 0.11569881936025439 1102 20-21 0.18876160849138302 1102 22-23 0.08553887069498245 1102 24-25 0.045230387889063195 1102 26-27 -0.07333769095522058 1102 28-29 0.08377219256833968 1102 30-31 -0.11108345904276717 1102 32-33 -0.05795734240352601 1102 34-35 -0.22436671617489168 1102 36-37 -0.35237907245101496 1102 38-39 -0.5845400991124592 1102 40-41 -0.3203167637335085 1102 42-43 0.13133903085842036 1102 44-45 0.07438855607349382 1102 46-47 0.092250727261316 1102 48-49 -0.15303044820976908 1102 50-51 -0.1934425937396611 1102 52-53 -0.1204717630088652 1102 54-55 4.580612514715199E-4 1102 56-57 -0.00695577982197193 1102 58-59 0.12146628389383096 1102 60-61 0.03864416914889546 1102 62-63 -0.12321424434431805 1102 64-65 -0.08848042347699447 1102 66-67 -0.14238215912730112 1102 68-69 -0.0459520013004493 1102 70-71 -0.16614919459149036 1102 72-73 -0.17739117361020362 1102 74-75 -0.08157578630665796 1102 76-77 -0.09270851105275568 1102 78-79 -0.06485575841508506 1102 80-81 -0.39740099469506873 1102 82-83 -0.24254409052336 1102 84-85 -0.21006199563806405 1102 86-87 -0.053286414065318866 1102 88-89 -0.3088790125454821 1102 90-91 -0.5627592006982951 1102 92-93 -0.4936577030459759 1102 94-95 -0.46723609312257963 1102 96-97 -0.6472242442548932 1102 98-99 -0.6024345258323507 1102 100-101 -0.6635770653016664 1103 1 0.19960721019729988 1103 2 0.1477600259928451 1103 3 0.09152725540871387 1103 4 0.09655223696250204 1103 5 0.135732734206222 1103 6 0.03151903536301859 1103 7 0.08921218836977118 1103 8 0.15304194526416381 1103 9 0.12822614872915494 1103 10-11 0.19591035583331262 1103 12-13 -0.07266107351201612 1103 14-15 0.19460407384412548 1103 16-17 0.1914808623579205 1103 18-19 0.19333962756795842 1103 20-21 0.23995507582807107 1103 22-23 0.27240418685913426 1103 24-25 0.06980639291418811 1103 26-27 0.1925359480732638 1103 28-29 0.29562573109431156 1103 30-31 0.30004771767414695 1103 32-33 0.23830260315761365 1103 34-35 0.21646373818020948 1103 36-37 0.22334459798450013 1103 38-39 -0.07729685162502165 1103 40-41 -0.09407487721758656 1103 42-43 0.10633117909962664 1103 44-45 -0.027782455233044345 1103 46-47 0.10946570841709047 1103 48-49 0.1340363964301048 1103 50-51 0.05721826262549712 1103 52-53 0.14214993925244102 1103 54-55 0.27542011108397446 1103 56-57 0.17222789898874424 1103 58-59 0.14736400164927943 1103 60-61 -0.15535970438543956 1103 62-63 -0.3100664742438184 1103 64-65 -0.26738929072992335 1103 66-67 0.007776184675044817 1103 68-69 0.0990568973595245 1103 70-71 0.3409762765140343 1103 72-73 0.5266302355187804 1103 74-75 0.4495629804437655 1103 76-77 0.39686173601592856 1103 78-79 0.43462733413097965 1103 80-81 0.3269695559749479 1103 82-83 0.16237111048166497 1103 84-85 0.35962236365842415 1103 86-87 0.22627545779398162 1103 88-89 0.10553027346792732 1103 90-91 0.29410977961995854 1103 92-93 0.520822248796577 1103 94-95 0.3646184923045581 1103 96-97 0.4329094973698986 1103 98-99 0.664773912191098 1103 100-101 0.3341001227217859 1104 1 0.12191940606104623 1104 2 0.031085755919853852 1104 3 0.1336881434865731 1104 4 0.07638420168269988 1104 5 0.08150143858578218 1104 6 0.14005766066715353 1104 7 0.14562946330894277 1104 8 0.10679422628606261 1104 9 0.15584552220847314 1104 10-11 0.04507778284061459 1104 12-13 0.13453174887241204 1104 14-15 0.09707137919691888 1104 16-17 -0.0122714186639854 1104 18-19 0.07284464581614003 1104 20-21 0.24010714395459587 1104 22-23 0.18723653174964738 1104 24-25 0.23263333938621145 1104 26-27 0.19947405634576398 1104 28-29 0.3354105546952795 1104 30-31 0.3500971398152686 1104 32-33 0.4152908939118731 1104 34-35 0.429330602535444 1104 36-37 0.24407338448084914 1104 38-39 0.2773545321949342 1104 40-41 0.4956548216875163 1104 42-43 0.452913447956071 1104 44-45 0.22298738965746878 1104 46-47 -0.013490876132792096 1104 48-49 -0.11063171488376611 1104 50-51 -0.11137776839640878 1104 52-53 0.11379873791424444 1104 54-55 -0.03336372407417798 1104 56-57 -0.03084957972171054 1104 58-59 0.11533465250085584 1104 60-61 0.2577903868554259 1104 62-63 0.11605692904085174 1104 64-65 0.07325281693430696 1104 66-67 -0.08843541812301225 1104 68-69 -0.05861874132661171 1104 70-71 0.13128383429992851 1104 72-73 0.09807374220977749 1104 74-75 0.10778036183062056 1104 76-77 0.18797715572394935 1104 78-79 0.19376092598013628 1104 80-81 0.14238736315256517 1104 82-83 0.08082457763494943 1104 84-85 0.14119550842728046 1104 86-87 0.13934951497159886 1104 88-89 -0.036586895023567934 1104 90-91 -0.15756106892018096 1104 92-93 -0.00969592334454461 1104 94-95 0.0665459558082091 1104 96-97 0.16716094201709808 1104 98-99 0.19372388177747624 1104 100-101 0.07601199924246416 1105 1 0.2339186939371345 1105 2 0.2091167333099193 1105 3 0.16817867817293575 1105 4 0.08241351745030556 1105 5 0.019154990303782427 1105 6 0.0559854987776518 1105 7 0.08829755422343055 1105 8 0.1566369655893638 1105 9 0.17132828287549984 1105 10-11 0.13825029485770557 1105 12-13 0.19974746307335067 1105 14-15 0.1658566144945297 1105 16-17 0.1561785249595431 1105 18-19 0.12323037960048566 1105 20-21 -0.06278882661094798 1105 22-23 0.29493340433881343 1105 24-25 0.15494358803614006 1105 26-27 0.11287385457732313 1105 28-29 0.23181729613495605 1105 30-31 0.2546844046660155 1105 32-33 -0.009728392777340389 1105 34-35 0.1832383824891579 1105 36-37 0.41435704717148525 1105 38-39 0.37218866666766104 1105 40-41 0.13969671831086572 1105 42-43 -0.009560589193057467 1105 44-45 0.12661922159622208 1105 46-47 0.045155495002454415 1105 48-49 0.03132425212115919 1105 50-51 0.17536485815394798 1105 52-53 0.16956990876463607 1105 54-55 0.16822371880754616 1105 56-57 0.1019852668749266 1105 58-59 -0.055595856074297956 1105 60-61 0.0938653972405703 1105 62-63 -0.17460534619504386 1105 64-65 0.0621140121156003 1105 66-67 -0.053345512479431534 1105 68-69 -0.06114381402258573 1105 70-71 -0.31803668080303993 1105 72-73 -0.38647316895276873 1105 74-75 -0.2886293467920069 1105 76-77 -0.05437047943123474 1105 78-79 0.03974039307407651 1105 80-81 -0.017244112724235094 1105 82-83 0.1618375738962996 1105 84-85 0.156992790487692 1105 86-87 0.03866112852568904 1105 88-89 0.09101680802076828 1105 90-91 0.1826323914085819 1105 92-93 0.09262738091039324 1105 94-95 -0.1325994955003189 1105 96-97 0.022765950621931097 1105 98-99 0.2489075504024818 1105 100-101 0.2219113523965781 1106 1 0.12840185305444152 1106 2 -0.013995926388105318 1106 3 0.042568922075375326 1106 4 0.021031403629166334 1106 5 -0.04216905150806127 1106 6 -0.02130834558936101 1106 7 0.08266456932215505 1106 8 0.07127111193082669 1106 9 -0.011133970318461195 1106 10-11 0.15625261773806898 1106 12-13 0.09846987886893288 1106 14-15 0.07964871670126428 1106 16-17 0.04110883854839642 1106 18-19 0.18136046090129554 1106 20-21 0.16912174249473821 1106 22-23 0.15651877019246996 1106 24-25 0.12590758339037933 1106 26-27 0.12579487664468303 1106 28-29 0.06102751680859342 1106 30-31 0.15860426529319938 1106 32-33 0.09581748411000035 1106 34-35 0.08071225008497862 1106 36-37 -0.05556165201550556 1106 38-39 0.09893082694640754 1106 40-41 0.06942958706812163 1106 42-43 -0.22558846375751784 1106 44-45 -0.0720160861854211 1106 46-47 -0.3573125653924336 1106 48-49 -0.236387710712755 1106 50-51 -0.19609274927927345 1106 52-53 -0.25357178693803206 1106 54-55 -0.6389772103445992 1106 56-57 -0.5574223986303011 1106 58-59 -0.4757758793031073 1106 60-61 -0.37344006152829934 1106 62-63 -0.35883879567239063 1106 64-65 -0.18126577882516415 1106 66-67 -0.34077292246781354 1106 68-69 -0.5328627454976242 1106 70-71 -0.21040021158120936 1106 72-73 -0.38888315733836976 1106 74-75 -0.4463819600324257 1106 76-77 -0.33044481160312245 1106 78-79 -0.5852015349166351 1106 80-81 -0.24918371783457616 1106 82-83 -0.1368848418992883 1106 84-85 -0.5214044220558591 1106 86-87 -0.4641263279203045 1106 88-89 -0.2564860955796959 1106 90-91 -0.24878456561813067 1106 92-93 -0.11582209644152286 1106 94-95 0.03760956373313107 1106 96-97 -0.034464014534865584 1106 98-99 -0.01848501638032829 1106 100-101 0.3251343489122611 1107 1 0.08822173955826429 1107 2 0.1834627462000995 1107 3 0.17909741948470526 1107 4 0.13804349343918432 1107 5 0.07359704767772257 1107 6 0.24176838769462705 1107 7 0.19575363914697164 1107 8 0.25676333991010125 1107 9 0.36490846738200133 1107 10-11 0.3378711718954861 1107 12-13 0.31059669850871074 1107 14-15 0.35626480355050916 1107 16-17 0.3662568770383956 1107 18-19 0.30070415692892993 1107 20-21 0.3367815006985353 1107 22-23 0.3760783556846974 1107 24-25 0.38724486441677897 1107 26-27 0.3894946052407917 1107 28-29 0.4589056317851501 1107 30-31 0.42916311059297385 1107 32-33 0.6019549131748931 1107 34-35 0.6082289044150997 1107 36-37 0.5573053600743023 1107 38-39 0.6764474812765329 1107 40-41 0.6677518175147057 1107 42-43 0.7162324096695727 1107 44-45 0.7132109061659193 1107 46-47 0.9557456630803074 1107 48-49 0.7547793260501336 1107 50-51 0.7606738433681528 1107 52-53 0.7411420053146216 1107 54-55 0.7458365078888036 1107 56-57 0.7439099477797626 1107 58-59 0.5582421320465158 1107 60-61 0.7027874242846721 1107 62-63 0.7008022109893446 1107 64-65 0.6362767822407136 1107 66-67 0.8835115581638107 1107 68-69 0.9753657272386249 1107 70-71 0.819016270037352 1107 72-73 0.8400275261059988 1107 74-75 0.9207349903746831 1107 76-77 0.8898858076912219 1107 78-79 0.8395887438891876 1107 80-81 0.9099453223825478 1107 82-83 0.8914028462329568 1107 84-85 0.8961751486325156 1107 86-87 0.966924745358746 1107 88-89 1.0319052194955631 1107 90-91 0.8346227869602103 1107 92-93 0.8045887103682503 1107 94-95 0.7161809923045581 1107 96-97 0.8716980876116907 1107 98-99 1.0839637869752146 1107 100-101 0.7896606257442365 1108 1 -0.026621575081115623 1108 2 -0.025688200551776674 1108 3 0.0017222401531782339 1108 4 0.05516565229431336 1108 5 0.03256125251285624 1108 6 0.13367149531725175 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70-71 -0.0268230457751244 2316 72-73 -0.013940529684894898 2316 74-75 0.010115458211977568 2316 76-77 -0.31265938417914185 2316 78-79 -0.05971386997114081 2316 80-81 0.0861289614540155 2316 82-83 -0.01144020621368469 2316 84-85 -0.07751457696119957 2316 86-87 0.0016223812018978379 2316 88-89 -0.021919256795989384 2316 90-91 0.1565797487823275 2316 92-93 0.10229322197500323 2316 94-95 0.05783331588459362 2316 96-97 0.12384063262520328 2316 98-99 0.147593583734416 2316 100-101 0.3424751406506452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 4.0 6 2.0 7 17.0 8 52.0 9 132.0 10 246.0 11 471.0 12 712.0 13 1002.0 14 1150.0 15 1248.0 16 1527.0 17 2317.0 18 2297.0 19 1369.0 20 1112.0 21 1128.0 22 1552.0 23 1883.0 24 2131.0 25 2667.0 26 2671.0 27 2270.0 28 2870.0 29 3642.0 30 4708.0 31 6247.0 32 8065.0 33 10676.0 34 15276.0 35 24444.0 36 43175.0 37 92933.0 38 152845.0 39 50983.0 40 62.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.618881315833946 20.135188418858785 13.886103757111472 24.3598265081958 2 16.000396495520278 21.78697827610056 39.193582179328615 23.01904304905055 3 18.35797223561429 25.69579929620301 31.226373012404306 24.71985545577839 4 13.222026947861746 19.194493263034566 37.21621378036141 30.367266008742284 5 13.450209399196646 37.10747506696494 35.54694980051319 13.895365733325225 6 29.576312997168213 37.364296028962194 18.639344520814888 14.420046453054706 7 25.123397966608767 32.931205774416576 23.34006925154712 18.605327007427533 8 23.730932733183295 34.75598629387077 22.351533829403298 19.16154714354264 9 24.580469441684745 16.29957038809252 21.82369916803525 37.296261002187485 10-11 23.26561370072248 26.39263419458468 29.901506908258597 20.440245196434244 12-13 24.277578403609912 24.768016328406425 29.095674819605804 21.858730448377862 14-15 21.67733941595562 26.63118470333136 26.809495649235448 24.88198023147757 16-17 21.450070063665567 29.091965882824717 27.64709990492969 21.810864148580027 18-19 21.26702846882892 27.82914197017723 29.54860336705798 21.355226193935874 20-21 21.904687883682634 27.162421235939615 29.382458227169405 21.550432653208347 22-23 21.49163416980371 27.53188297074269 29.196713592812618 21.779769266640987 24-25 21.52317358618934 27.62976780231094 29.394285508314017 21.452773103185706 26-27 21.52020500689927 27.756582467404467 29.3684773732083 21.35473515248796 28-29 21.10689185550996 27.651344047564063 29.651278712309725 21.59048538461625 30-31 22.143173341954117 27.37083749635325 29.271089474045848 21.214899687646785 32-33 21.212558122769707 27.81140738204232 29.321585625202758 21.65444886998522 34-35 21.624520226798804 27.85889626534514 29.37501901067865 21.141564497177406 36-37 21.533397617288177 27.798154389632863 29.269776327884216 21.398671665194744 38-39 21.396565357555605 27.496401127308857 29.430330555611878 21.676702959523663 40-41 21.507854441087748 27.430168352899038 29.606162801961748 21.455814404051463 42-43 21.851070875827062 27.25298441727549 29.35193257773903 21.544012129158414 44-45 21.544575333022443 27.575587590591343 29.13791510940798 21.741921966978232 46-47 21.869881884885636 27.31617589081955 28.838628576062934 21.97531364823188 48-49 21.770420082498102 27.378804160499588 29.012545929275113 21.8382298277272 50-51 21.57160911849584 27.68800308185154 28.901369486515772 21.839018313136844 52-53 21.723223598692464 27.82001806757996 28.942370727817092 21.514387605910485 54-55 21.770307441725294 27.751983040805246 28.5066762186042 21.971033298865255 56-57 21.766365014677092 28.054310875016053 28.29705174041258 21.882272369894277 58-59 21.467577406913456 28.291370796236887 28.836891287892442 21.404160508957215 60-61 21.797072652027673 28.650680472106387 28.138158028129034 21.414088847736902 62-63 21.54438345084583 28.82279851347348 28.11535788272482 21.517460152955874 64-65 21.593801956375795 28.713036347497756 28.115692909376815 21.57746878674963 66-67 21.505903238924315 28.686665923199882 28.05618355969644 21.75124727817936 68-69 21.395526448294625 28.610837948929372 28.260289202715626 21.733346400060377 70-71 21.538943294382154 28.4814446854957 28.15129458040186 21.82831743972029 72-73 21.666619730209245 28.454757948689064 27.95843307330536 21.920189247796333 74-75 21.625171504076672 28.449963615227087 28.02314657318521 21.901718307511032 76-77 21.507694595197382 28.418637343624333 27.953987875183323 22.11968018599496 78-79 21.577475323083718 28.310277514634937 27.899249019738154 22.212998142543196 80-81 21.584979542546094 28.232638200465026 28.05104450172131 22.13133775526757 82-83 21.673384598399863 28.371789643495248 27.91002102123077 22.044804736874124 84-85 21.694127135569143 28.193849470747196 27.768960339550848 22.343063054132813 86-87 21.662660319591602 28.149337995264474 27.961111023499647 22.226890661644276 88-89 21.624784714110476 28.211716283329185 27.800349864210784 22.363149138349556 90-91 21.746652879436077 27.869917929932935 28.04248359387144 22.34094559675955 92-93 21.765463888494647 27.88884157976431 27.917339695284184 22.42835483645686 94-95 21.70373674499706 28.037752681413597 27.83837851354731 22.420132060042036 96-97 21.666452649198337 27.885237075034524 27.94245858761988 22.505851688147263 98-99 21.696527735537487 27.93648862666117 27.77608816618569 22.590895471615653 100-101 21.69868704436294 28.001234556776094 27.797465103495504 22.502613295365457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 888.0 1 518.5 2 146.5 3 131.5 4 95.0 5 96.0 6 144.0 7 256.5 8 389.0 9 439.0 10 342.0 11 219.5 12 203.5 13 231.5 14 267.5 15 286.0 16 315.0 17 371.5 18 411.5 19 425.5 20 469.5 21 553.0 22 699.0 23 880.5 24 1088.5 25 1458.0 26 2011.0 27 2780.0 28 3751.0 29 4801.0 30 6035.5 31 7389.0 32 8693.0 33 9950.0 34 10973.5 35 12029.5 36 13244.5 37 14088.0 38 14706.5 39 15214.5 40 15661.0 41 16355.0 42 16971.0 43 17339.5 44 17658.5 45 17998.5 46 17869.5 47 17378.0 48 17268.5 49 16822.0 50 15825.0 51 15111.0 52 14412.5 53 13425.0 54 12307.5 55 11171.5 56 9866.0 57 8475.0 58 7456.0 59 6399.5 60 5171.0 61 4072.5 62 3152.0 63 2430.0 64 1763.0 65 1295.0 66 963.0 67 707.0 68 548.0 69 383.0 70 268.5 71 192.5 72 143.0 73 108.5 74 93.0 75 75.5 76 54.0 77 39.5 78 30.5 79 23.5 80 13.5 81 8.5 82 8.5 83 8.0 84 7.5 85 5.5 86 2.0 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0144180189191442 2 0.0 3 0.0024780970017279098 4 0.01554442664720234 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.379223184174422E-4 16-17 0.001576970819281397 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.379223184174422E-4 28-29 0.005519397867484889 30-31 1.1264077280581406E-4 32-33 2.2528154561162812E-4 34-35 0.013404251963891875 36-37 0.005632038640290704 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.2528154561162812E-4 60-61 0.0013516892736697688 62-63 0.0079974948692128 64-65 0.001576970819281397 66-67 0.0050688347762616335 68-69 0.00304130086575698 70-71 0.0 72-73 0.005969960958708146 74-75 0.0049561940034558195 76-77 0.0013516892736697688 78-79 5.632038640290704E-4 80-81 0.009236543370076754 82-83 0.011714640371804664 84-85 0.0019148931376988393 86-87 0.0022528154561162815 88-89 5.632038640290704E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0011264077280581407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 443889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.90211543209792 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66751633303542 52.19417345739898 2 17.6400558964281 24.661544470015524 3 4.900264869677982 10.276166417042534 4 1.4840418799517205 4.149506671938111 5 0.6159560599249988 2.1528315800988738 6 0.26917337647325607 1.1289473060088655 7 0.13113245131816742 0.6416505024255549 8 0.07962263252326113 0.445263235971881 9 0.049198804036193935 0.30951904309732736 >10 0.1523762747385391 1.561856744992357 >50 0.00581532110216607 0.27709971186585 >100 0.0022615137020909767 0.36653041797199465 >500 0.0016153669300649814 0.776647016269679 >1k 9.692201580389889E-4 1.0582634249025054 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2251 0.507108759171775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.28881094147410724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1153 0.25974962209020724 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 949 0.2137921867854351 RNA PCR Primer, Index 17 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 666 0.15003750937734434 RNA PCR Primer, Index 17 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 626 0.1410262475528792 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.1351689273669769 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 598 0.13471836427575362 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 472 0.10633288952868848 RNA PCR Primer, Index 17 (96% over 28bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.2528154561162814E-4 0.0 20-21 0.0 0.0 0.0 3.379223184174422E-4 0.0 22-23 0.0 0.0 0.0 0.0013516892736697688 0.0 24-25 0.0 0.0 0.0 0.0018022523648930251 0.0 26-27 0.0 0.0 0.0 0.0023654562289220954 0.0 28-29 0.0 0.0 0.0 0.004392990139426748 6.758446368348844E-4 30-31 0.0 0.0 0.0 0.0079974948692128 6.758446368348844E-4 32-33 0.0 0.0 0.0 0.012841048099862803 6.758446368348844E-4 34-35 0.0 0.0 0.0 0.018022523648930252 6.758446368348844E-4 36-37 0.0 0.0 0.0 0.02489361079008491 6.758446368348844E-4 38-39 0.0 0.0 0.0 0.03356695029613259 7.884854096406985E-4 40-41 0.0 0.0 0.0 0.039874833573258175 9.011261824465126E-4 42-43 0.0 0.0 0.0 0.04933665848894656 9.011261824465126E-4 44-45 0.0 0.0 0.0 0.054405493265208195 9.011261824465126E-4 46-47 0.0 0.0 0.0 0.062402988134420995 9.011261824465126E-4 48-49 0.0 0.0 0.0 0.07704628859917682 9.011261824465126E-4 50-51 0.0 0.0 0.0 0.09315391911040824 9.011261824465126E-4 52-53 0.0 0.0 0.0 0.11500622903473617 9.011261824465126E-4 54-55 0.0 0.0 0.0 0.1352815681397827 9.011261824465126E-4 56-57 0.0 0.0 0.0 0.1698622853911676 9.011261824465126E-4 58-59 0.0 0.0 0.0 0.20703374041708628 9.011261824465126E-4 60-61 0.0 0.0 0.0 0.23710882675623862 9.011261824465126E-4 62-63 0.0 0.0 0.0 0.25851057358934326 9.011261824465126E-4 64-65 0.0 0.0 0.0 0.31280342608174566 9.011261824465126E-4 66-67 0.0 0.0 0.0 0.3916519670458155 9.011261824465126E-4 68-69 0.0 0.0 0.0 0.4830036337913307 9.011261824465126E-4 70-71 0.0 0.0 0.0 0.5798746984043308 9.011261824465126E-4 72-73 0.0 0.0 0.0 0.6784353746094182 9.011261824465126E-4 74-75 0.0 0.0 0.0 0.7889359727319216 9.011261824465126E-4 76-77 0.0 0.0 0.0 0.9205003953691125 9.011261824465126E-4 78-79 0.0 0.0 0.0 1.0600623128755162 9.011261824465126E-4 80-81 0.0 0.0 0.0 1.2048057059309873 9.011261824465126E-4 82-83 0.0 0.0 0.0 1.3616016616766804 9.011261824465126E-4 84-85 0.0 0.0 0.0 1.536870704162527 9.011261824465126E-4 86-87 0.0 0.0 0.0 1.7455940561717007 9.011261824465126E-4 88-89 0.0 0.0 0.0 1.9975714649383067 9.011261824465126E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 765 0.0 56.524113 1 GTATCAA 1175 0.0 36.788372 2 TCAACGC 1295 0.0 33.37565 5 ATCAACG 1335 0.0 32.379276 4 CAACGCA 1340 0.0 32.25482 6 CCTAGCG 30 0.0039520543 31.664074 84-85 AACGCAG 1440 0.0 30.674574 7 TATCAAC 1415 0.0 30.548645 3 CGCAGAG 1585 0.0 27.26906 9 ACGCAGA 1635 0.0 27.016136 8 GTATAGG 90 8.919625E-4 26.398624 1 TCGACTG 45 8.9502113E-4 26.38673 12-13 CCTAGAC 125 2.2524036E-4 22.800705 3 GTGGTAT 345 0.0 22.037113 1 CAGAGTA 1715 0.0 21.740204 10-11 GAGTACT 1240 0.0 21.25834 12-13 ACTAACC 145 6.136614E-4 19.655779 3 GCAGAGT 1865 0.0 19.354986 9 GTACTTT 1370 0.0 19.067781 14-15 TGGTATC 375 9.094947E-12 19.000586 2 >>END_MODULE