FastQCFastQC Report
Fri 10 Feb 2017
SRR2031690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368703
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.17092029373477666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66250.15164683889016947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.054.9797061
GTATCAA102350.039.5902981
TCAACGC117800.033.710384
ATCAACG121100.032.8313673
CAACGCA121400.032.7096065
TATCAAC127200.031.5571062
AACGCAG128750.030.9118336
ACGCAGA145300.027.1957267
CGCAGAG150350.026.1273168
GTGGTAT31150.023.9424821
GCAGAGT171100.021.9038129
GAGTACT98700.021.70822512-13
TGGTATC32950.020.760352
CAGAGTA159900.020.60324110-11
GTACATG99500.020.1473051
TACATGG101300.019.4141542
GTACTTT109200.019.14310514-15
AGAGTAC146700.018.53885510-11
ACATGGG104550.017.9919743
CATGGGG71000.016.5249924
Produced by FastQC (version 0.11.5)