FastQCFastQC Report
Fri 10 Feb 2017
SRR2031690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368703
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99250.2271841322241407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86040.1969463248016631No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT45290.1036692125786532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.054.728881
GTATCAA110000.040.125951
TCAACGC131200.032.804024
ATCAACG132350.032.5556223
CAACGCA134050.032.065645
TATCAAC135400.031.9275342
AACGCAG140650.030.6622666
GTGGTAT30850.029.8778171
ACGCAGA157950.027.1835987
CGCAGAG162750.026.1192198
TGGTATC34550.024.8870352
GCAGAGT184950.022.4447759
GAGTACT117050.021.77089712-13
CAGAGTA175250.020.67971410-11
GTACTTT127450.019.56579414-15
AGAGTAC159000.019.40260710-11
GTACATG95400.019.3234921
TACATGG97800.017.8266542
AGTACTT129100.017.31057212-13
ACATGGG97000.016.9452113
Produced by FastQC (version 0.11.5)