##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031690_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4368703 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78744881489998 34.0 31.0 34.0 31.0 34.0 2 32.953590802579164 34.0 31.0 34.0 31.0 34.0 3 33.013988362221006 34.0 31.0 34.0 31.0 34.0 4 36.395225539479334 37.0 37.0 37.0 35.0 37.0 5 36.362291279585726 37.0 37.0 37.0 35.0 37.0 6 36.39042663234374 37.0 37.0 37.0 35.0 37.0 7 36.34042506437265 37.0 37.0 37.0 35.0 37.0 8 36.327772796640105 37.0 37.0 37.0 35.0 37.0 9 38.18610374749668 39.0 39.0 39.0 37.0 39.0 10-11 38.155242414052864 39.0 39.0 39.0 37.0 39.0 12-13 38.10631713806134 39.0 39.0 39.0 37.0 39.0 14-15 39.61752698684255 41.0 40.0 41.0 37.0 41.0 16-17 39.59409760745924 41.0 40.0 41.0 37.0 41.0 18-19 39.60203611918686 41.0 40.0 41.0 37.0 41.0 20-21 39.59013922438765 41.0 40.0 41.0 37.0 41.0 22-23 39.502554076118244 41.0 39.0 41.0 37.0 41.0 24-25 39.43953583019949 41.0 39.0 41.0 36.0 41.0 26-27 39.312162442720414 41.0 39.0 41.0 36.0 41.0 28-29 39.21391760895625 41.0 39.0 41.0 36.0 41.0 30-31 39.059421068449836 40.0 39.0 41.0 35.5 41.0 32-33 38.87178769076314 40.0 38.0 41.0 35.0 41.0 34-35 38.73279884212775 40.0 38.0 41.0 35.0 41.0 36-37 38.63056426587021 40.0 38.0 41.0 35.0 41.0 38-39 38.7012416499817 40.0 38.0 41.0 35.0 41.0 40-41 38.621723999090804 40.0 38.0 41.0 35.0 41.0 42-43 38.524596316114874 40.0 38.0 41.0 35.0 41.0 44-45 38.347779878833606 40.0 38.0 41.0 34.0 41.0 46-47 38.15249789239506 40.0 38.0 41.0 34.0 41.0 48-49 37.92376158324336 40.0 38.0 41.0 33.0 41.0 50-51 37.786209659937974 40.0 38.0 41.0 33.0 41.0 52-53 37.8468371505227 40.0 38.0 41.0 33.5 41.0 54-55 37.77176166473207 40.0 37.5 41.0 33.5 41.0 56-57 37.597263993455265 40.0 37.0 41.0 33.0 41.0 58-59 37.413402787967044 40.0 37.0 41.0 33.0 41.0 60-61 37.160060091061354 39.5 36.0 41.0 33.0 41.0 62-63 36.89231311901953 39.0 36.0 41.0 32.5 41.0 64-65 36.57130159683549 39.0 35.0 41.0 32.0 41.0 66-67 36.227129768263026 38.5 35.0 40.5 31.5 41.0 68-69 35.84195892922911 37.5 35.0 40.0 31.0 41.0 70-71 35.483712900602306 37.0 35.0 39.5 31.0 41.0 72-73 35.087390811414735 36.5 35.0 39.0 30.5 41.0 74-75 34.69401124315386 36.0 35.0 39.0 30.0 40.5 76-77 33.7640241279849 35.0 33.5 37.0 29.0 39.0 78-79 33.94388231472819 35.0 34.0 37.0 30.0 39.0 80-81 33.71317139205847 35.0 34.0 37.0 30.0 39.0 82-83 33.44024187499127 35.0 34.0 36.0 30.0 37.5 84-85 33.151406035155055 35.0 34.0 36.0 29.0 37.0 86-87 32.90705273395788 35.0 34.0 35.5 29.0 37.0 88-89 32.684529252732446 35.0 34.0 35.0 29.0 36.0 90-91 32.49062971321237 35.0 34.0 35.0 29.0 36.0 92-93 32.328406508750994 35.0 34.0 35.0 29.0 36.0 94-95 32.15431536545286 35.0 34.0 35.0 28.5 36.0 96-97 31.994724177862402 35.0 34.0 35.0 27.0 35.0 98-99 31.790656403056012 35.0 33.5 35.0 27.0 35.0 100-101 31.093647931663014 34.5 32.0 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1638820093529887 1101 2 0.178470146873984 1101 3 0.13657662588234132 1101 4 0.10529271979896748 1101 5 0.09899753246011045 1101 6 0.1096871238291115 1101 7 0.1450854727324682 1101 8 0.14499677995893023 1101 9 0.0338362770649141 1101 10-11 0.1124859525110864 1101 12-13 0.10428245710824058 1101 14-15 0.07107866676376062 1101 16-17 0.07187797328473522 1101 18-19 0.13102808871602178 1101 20-21 0.08904167972317367 1101 22-23 -0.016307258184603768 1101 24-25 0.06646159799475271 1101 26-27 0.06540349373554477 1101 28-29 0.058092981897303275 1101 30-31 0.11846307636873377 1101 32-33 0.07306009596585028 1101 34-35 -0.10294638524964483 1101 36-37 0.006886460446047238 1101 38-39 0.015799868834967867 1101 40-41 -0.14579861457563226 1101 42-43 -0.0931467531911565 1101 44-45 -0.06486091376235237 1101 46-47 0.0020942030667825406 1101 48-49 -0.20491982431138922 1101 50-51 -0.11592424701242265 1101 52-53 -0.1877763048892902 1101 54-55 -0.1424808625092595 1101 56-57 -0.2990181043378257 1101 58-59 -0.27001583893057557 1101 60-61 -0.17251770505587416 1101 62-63 -0.09813399613204155 1101 64-65 -0.10877771626037003 1101 66-67 -0.4138015027764581 1101 68-69 -0.4008219843079317 1101 70-71 -0.3508466717877994 1101 72-73 -0.40803240205600844 1101 74-75 -0.44580791155040345 1101 76-77 -0.48306516950837874 1101 78-79 -0.3756883977932546 1101 80-81 -0.3518161408239209 1101 82-83 -0.2751659794235266 1101 84-85 -0.29988248548027485 1101 86-87 -0.2615294966101729 1101 88-89 -0.3625299414585541 1101 90-91 -0.43471658293749016 1101 92-93 -0.460098493479304 1101 94-95 -0.387911508799629 1101 96-97 -0.33690765337414064 1101 98-99 -0.6428612913261738 1101 100-101 -0.8356626046826712 1102 1 0.11013936226900967 1102 2 0.07136912967179398 1102 3 0.10809512263296739 1102 4 0.041880506160978825 1102 5 0.09628568201650012 1102 6 0.10022061869914722 1102 7 0.107321706733984 1102 8 0.04990468114078794 1102 9 0.07378245986546972 1102 10-11 0.14845391460348623 1102 12-13 0.09745357906274421 1102 14-15 0.17029690660154273 1102 16-17 0.11190433442138215 1102 18-19 0.11508520069564554 1102 20-21 0.1424699961452589 1102 22-23 0.10802463288182906 1102 24-25 0.09768061401936734 1102 26-27 0.1560231745593157 1102 28-29 0.14559156388897776 1102 30-31 -0.009188730798989297 1102 32-33 0.10944599215895323 1102 34-35 0.04052325542244262 1102 36-37 0.09557459020953729 1102 38-39 -0.05318350539578631 1102 40-41 0.07137238709491811 1102 42-43 0.11593778458287574 1102 44-45 0.0875256304375327 1102 46-47 0.03424693315722038 1102 48-49 0.036899024368509004 1102 50-51 -0.04805310455436995 1102 52-53 0.052992625423136985 1102 54-55 0.20786668442188017 1102 56-57 0.15797475600671618 1102 58-59 0.08132168179340482 1102 60-61 0.1406855579819677 1102 62-63 0.1277820701457344 1102 64-65 0.04358194829216444 1102 66-67 0.02861844325104812 1102 68-69 0.07321346076769686 1102 70-71 -0.021368390937659854 1102 72-73 -0.01560623126806604 1102 74-75 -0.030047838032430718 1102 76-77 -0.1177986615884592 1102 78-79 -0.110992633288717 1102 80-81 -0.23885720292334156 1102 82-83 -0.13889024701400388 1102 84-85 -0.10097849076329624 1102 86-87 -0.042845864992813176 1102 88-89 -0.12112596713983947 1102 90-91 -0.25664359110705703 1102 92-93 -0.15713844880842487 1102 94-95 -0.18045044250474263 1102 96-97 -0.42749630909698766 1102 98-99 -0.45518584980905175 1102 100-101 -0.4044893399892757 1103 1 0.18378450192844298 1103 2 0.10233975147141905 1103 3 0.15362835645841955 1103 4 0.12282817769661847 1103 5 0.07007337855562668 1103 6 0.08355019174717171 1103 7 0.11170078536710548 1103 8 0.13032995881265208 1103 9 0.15599539836819787 1103 10-11 0.1517848016640997 1103 12-13 0.10263230436392234 1103 14-15 0.13006570717713828 1103 16-17 0.19615267586581808 1103 18-19 0.2022927678668296 1103 20-21 0.1979709403268899 1103 22-23 0.13193901988756096 1103 24-25 0.08170569534717487 1103 26-27 0.1436756627988629 1103 28-29 0.23382002764962095 1103 30-31 0.17360355495803503 1103 32-33 0.10531632544739722 1103 34-35 0.15538895736238345 1103 36-37 0.10388735757593537 1103 38-39 -0.05456572333729781 1103 40-41 0.04135535566773996 1103 42-43 0.05470592153714904 1103 44-45 0.13721335335325335 1103 46-47 0.14937113464249308 1103 48-49 0.2587092957210899 1103 50-51 0.04724177342324509 1103 52-53 0.03607384623717991 1103 54-55 0.21413948897882307 1103 56-57 0.2101232982587291 1103 58-59 0.22988689371454285 1103 60-61 0.07272490360369233 1103 62-63 -0.037028601897212354 1103 64-65 0.18993623447473595 1103 66-67 0.13743454937188204 1103 68-69 0.1493742987352391 1103 70-71 0.17743301249972632 1103 72-73 0.15647010836809727 1103 74-75 0.3152049253919387 1103 76-77 0.3011066593083882 1103 78-79 0.282266610542635 1103 80-81 0.22532978839048923 1103 82-83 0.2668708055113669 1103 84-85 0.1847971559078374 1103 86-87 0.2208847156703797 1103 88-89 0.25810949974277264 1103 90-91 0.18609615855105943 1103 92-93 0.16901941882272808 1103 94-95 0.13391075823560072 1103 96-97 0.12746910751725693 1103 98-99 0.16531225216205314 1103 100-101 0.029390682953923886 1104 1 0.19462463720945777 1104 2 0.16029112928105604 1104 3 0.17446603701843344 1104 4 0.10346919362049078 1104 5 0.16323830617567126 1104 6 0.1404625394928658 1104 7 0.1579077423645714 1104 8 0.14056230891637966 1104 9 0.06454771496046163 1104 10-11 0.15380718609960553 1104 12-13 0.19374312643968494 1104 14-15 0.29165512499029944 1104 16-17 0.18866312558505882 1104 18-19 0.24550777241913835 1104 20-21 0.31696449223955625 1104 22-23 0.2715790211726272 1104 24-25 0.25561126251093214 1104 26-27 0.3223850597016735 1104 28-29 0.277645156694021 1104 30-31 0.2866963568240024 1104 32-33 0.34355170855074135 1104 34-35 0.2948145526478214 1104 36-37 0.2942181273321509 1104 38-39 0.28372904717124214 1104 40-41 0.33793917628034364 1104 42-43 0.4015716295838061 1104 44-45 0.3410546330270634 1104 46-47 0.30765285489350447 1104 48-49 0.2543349017985719 1104 50-51 0.3533125448700076 1104 52-53 0.3999809323960122 1104 54-55 0.39766279026978424 1104 56-57 0.3262951374623455 1104 58-59 0.3260436802071496 1104 60-61 0.45904477776726793 1104 62-63 0.38310974829946076 1104 64-65 0.45269440515550485 1104 66-67 0.3659749073868639 1104 68-69 0.3969809769487611 1104 70-71 0.3510928580363313 1104 72-73 0.23798113106350627 1104 74-75 0.3387622933446295 1104 76-77 0.3952176058356969 1104 78-79 0.37571150735858083 1104 80-81 0.4050639299414982 1104 82-83 0.37198448529410655 1104 84-85 0.40074732164050175 1104 86-87 0.4148583288231933 1104 88-89 0.39243891332021974 1104 90-91 0.3674530048053697 1104 92-93 0.3740720580844723 1104 94-95 0.4869847270578376 1104 96-97 0.4443428659259254 1104 98-99 0.4878007092030323 1104 100-101 0.5304202621860483 1105 1 0.15963906531109728 1105 2 0.12650976568658479 1105 3 0.08522197586886904 1105 4 0.06761009932276352 1105 5 0.06402833143497588 1105 6 0.07681550090914158 1105 7 0.11885642119722206 1105 8 0.12680512676620737 1105 9 0.06265397220190749 1105 10-11 0.10225135005183716 1105 12-13 0.14400645318072236 1105 14-15 0.14923635605821062 1105 16-17 0.1481794726797503 1105 18-19 0.11176664117549961 1105 20-21 0.10645466128448788 1105 22-23 0.13780981735583708 1105 24-25 0.16156195746474822 1105 26-27 0.15508417305872513 1105 28-29 0.18577707556999457 1105 30-31 0.13567900377041298 1105 32-33 0.02194093132477093 1105 34-35 0.05871184245855687 1105 36-37 0.14572088069613187 1105 38-39 0.12090232820651892 1105 40-41 0.06688590234276859 1105 42-43 0.0035884713262461787 1105 44-45 0.07794941011732703 1105 46-47 0.07459324165849779 1105 48-49 0.1809888942739235 1105 50-51 0.26026589605969974 1105 52-53 0.2643884524829119 1105 54-55 0.23069538177660576 1105 56-57 0.11015980303993445 1105 58-59 0.1519943571464779 1105 60-61 0.20223324561427347 1105 62-63 0.07772990236534127 1105 64-65 0.05169310000604099 1105 66-67 0.11534483574625654 1105 68-69 0.10421244176962574 1105 70-71 0.12047384728978017 1105 72-73 0.087220244234409 1105 74-75 0.04686067525352655 1105 76-77 0.08192483712038268 1105 78-79 0.1644109305647845 1105 80-81 0.0842988127107347 1105 82-83 0.06146864951055164 1105 84-85 0.15621500320323634 1105 86-87 0.058876453797481076 1105 88-89 0.0907866847632377 1105 90-91 0.07957062858665154 1105 92-93 0.10717424555341637 1105 94-95 0.05054247384202171 1105 96-97 0.1358968945203145 1105 98-99 0.048724678166475854 1105 100-101 0.029169576509698203 1106 1 0.0968083440607117 1106 2 0.10977147917071761 1106 3 0.03471618790938891 1106 4 0.0848890050555724 1106 5 0.039814828430387195 1106 6 0.02442364420971188 1106 7 0.08593899526489679 1106 8 0.02532820478300124 1106 9 0.007932306880981344 1106 10-11 -0.012680096202139168 1106 12-13 8.166755071812304E-4 1106 14-15 0.05691624466323475 1106 16-17 0.08530696205177435 1106 18-19 0.04974359620688773 1106 20-21 0.1234311966215742 1106 22-23 0.019082507477754973 1106 24-25 0.059431419413876085 1106 26-27 0.07537222761596496 1106 28-29 0.04011007935095279 1106 30-31 0.06097312954882739 1106 32-33 0.10275017463559522 1106 34-35 0.08061688165926029 1106 36-37 0.19948423630822987 1106 38-39 0.05770584100011433 1106 40-41 0.15487981125784245 1106 42-43 0.08571052256147738 1106 44-45 0.036289582504458906 1106 46-47 0.016713908239438524 1106 48-49 0.0884760115874812 1106 50-51 0.13723933334897254 1106 52-53 0.2281380115018905 1106 54-55 0.06874002456426354 1106 56-57 0.21016384548702405 1106 58-59 0.20601417001602584 1106 60-61 0.2551396431004278 1106 62-63 0.25145560513838205 1106 64-65 0.3199450390157281 1106 66-67 0.2698432429872568 1106 68-69 0.29751386881152797 1106 70-71 0.18416611507588954 1106 72-73 0.29750703494612196 1106 74-75 0.2591951745075818 1106 76-77 0.25940836037322157 1106 78-79 0.18535484312154438 1106 80-81 0.1590348891232054 1106 82-83 0.16678151110192374 1106 84-85 0.24456268431968198 1106 86-87 0.22238794985487687 1106 88-89 0.3061693691734533 1106 90-91 0.313908353202379 1106 92-93 0.32285352128629796 1106 94-95 0.3728859849652011 1106 96-97 0.3854729312656957 1106 98-99 0.4539914117193824 1106 100-101 0.5872331142526193 1107 1 0.17610512126229594 1107 2 0.14601727957819577 1107 3 0.13461589241998695 1107 4 0.10383608231907715 1107 5 0.06524363287516621 1107 6 0.07741309444350719 1107 7 0.11293219945842026 1107 8 0.10853410737494329 1107 9 0.13472814334386385 1107 10-11 0.1512181376077848 1107 12-13 0.17561717143764355 1107 14-15 0.26055338037997444 1107 16-17 0.22531792859145838 1107 18-19 0.2490865033368621 1107 20-21 0.2219887555882778 1107 22-23 0.24610131025844595 1107 24-25 0.2435459781511966 1107 26-27 0.2948205199690648 1107 28-29 0.3018543213554281 1107 30-31 0.2647718024700936 1107 32-33 0.22101902051786482 1107 34-35 0.25854149213346744 1107 36-37 0.3292299070361864 1107 38-39 0.23123971284076816 1107 40-41 0.28131696766333647 1107 42-43 0.3272318807033301 1107 44-45 0.3929903406424131 1107 46-47 0.39855975002443245 1107 48-49 0.35192726333215774 1107 50-51 0.4525460891272104 1107 52-53 0.4604466892355319 1107 54-55 0.4481836094758691 1107 56-57 0.5356513805117373 1107 58-59 0.46369262177749704 1107 60-61 0.4920911099781762 1107 62-63 0.5134704500701659 1107 64-65 0.5703260194528639 1107 66-67 0.6203184472137551 1107 68-69 0.49304284881089444 1107 70-71 0.5502067969652771 1107 72-73 0.5633146310176542 1107 74-75 0.5618070174709402 1107 76-77 0.5734177587651885 1107 78-79 0.5450163046172207 1107 80-81 0.568173209945833 1107 82-83 0.5868447692211376 1107 84-85 0.64859571736622 1107 86-87 0.5996944462740714 1107 88-89 0.6014989200625465 1107 90-91 0.5748700205361814 1107 92-93 0.6546782995193254 1107 94-95 0.7153394240210744 1107 96-97 0.729100419725718 1107 98-99 0.7491142624500036 1107 100-101 0.9113174119368246 1108 1 0.021241725530479982 1108 2 -0.0341525621119132 1108 3 0.03418148738519733 1108 4 0.05362899755279926 1108 5 0.03381875120409461 1108 6 0.015950560110198353 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quality scores pass #Quality Count 2 3.0 3 9.0 4 20.0 5 29.0 6 85.0 7 174.0 8 554.0 9 1183.0 10 2144.0 11 3524.0 12 5503.0 13 7813.0 14 9856.0 15 11683.0 16 14257.0 17 20021.0 18 20321.0 19 12310.0 20 9211.0 21 9220.0 22 10071.0 23 11232.0 24 13034.0 25 16129.0 26 18764.0 27 21838.0 28 27804.0 29 36146.0 30 46971.0 31 60759.0 32 79889.0 33 107135.0 34 153752.0 35 242118.0 36 440256.0 37 965444.0 38 1526073.0 39 463079.0 40 289.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.80352320740311 19.836580320726576 14.50465269884006 23.855243773030256 2 15.929304418267847 21.259948318757306 39.07498403988552 23.73576322308932 3 18.46288442310439 24.950052922842524 30.935232695695486 25.651829958357595 4 13.525258322845806 18.275014898508136 37.21854525998498 30.98118151866108 5 13.93205260233987 36.71036918737667 35.136515345630045 14.221062864653423 6 30.675969503992373 36.506098034130495 18.359018683577254 14.458913778299875 7 26.284437280355288 32.42962499396274 22.95850736477165 18.32743036091032 8 24.41566753336173 34.306818293667476 21.74267740333916 19.534836769631628 9 25.114707957945413 16.28810656160421 21.808875540406387 36.78830994004399 10-11 23.801051204682764 26.195915857845193 29.410680792601557 20.59235214487049 12-13 24.508778579999277 24.338773850905827 28.923018782237836 22.22942878685706 14-15 22.154350510947253 26.172560049048922 26.76463556259706 24.908453877406767 16-17 21.63541401757937 28.56085974523439 27.78077769229484 22.0229485448914 18-19 21.41005004697054 27.615975110193062 29.562242371636753 21.41173247119964 20-21 22.19384105534297 26.90295037222718 29.405043098603866 21.498165473825985 22-23 22.04907268816397 26.867070157893547 29.080301407534453 22.00355574640803 24-25 22.013043688252555 26.972765143338883 29.1749748151797 21.839216353228867 26-27 21.836318116139 27.137979084107066 29.22369704859869 21.80200575115524 28-29 21.762615433761283 27.072130656315824 29.309596715645313 21.855657194277583 30-31 22.153558266761276 26.904227802321056 29.082404376588002 21.85980955432966 32-33 21.950178010885864 27.12944957113608 28.84482511762384 22.075547300354216 34-35 22.10691709076233 27.18078419419001 28.86679266011655 21.845506054931114 36-37 22.150053181571945 27.014441603795515 28.83685326704703 21.998651947585508 38-39 21.948974329452014 26.975695074716683 28.987951343911455 22.08737925191985 40-41 22.222682567343213 26.980410433027835 28.80228983293211 21.99461716669684 42-43 22.26068011489909 27.024771425294876 28.56148609781896 22.153062361987068 44-45 22.271953483676963 27.127078677584628 28.469296264818183 22.131671573920222 46-47 22.370529651477796 27.052342537361774 28.227657041460592 22.34947076969984 48-49 22.333985624566377 27.061544353095186 28.230369516993946 22.374100505344494 50-51 22.31428870307732 27.262141647074657 28.10060560308174 22.322964046766284 52-53 22.382077701322338 27.31591046587511 28.031843776058935 22.270168056743614 54-55 22.42831568087828 27.2244302256299 27.949977373147135 22.39727672034469 56-57 22.36141939609994 27.384672292897918 27.8904059168133 22.363502394188846 58-59 22.23463121930719 27.353277661470454 28.152646992442136 22.259444126780224 60-61 22.400535091211122 27.339805122967288 28.037625775391252 22.22203401043034 62-63 22.262297994186692 27.485610380167998 27.92981542048961 22.3222762051557 64-65 22.281943810660817 27.42102197623978 27.91656923852915 22.380464974570256 66-67 22.249422054434813 27.444637061172838 27.81878227516092 22.487158609231425 68-69 22.223399828135666 27.388520524602832 27.898974536701093 22.48910511056041 70-71 22.28282130018232 27.254878173001316 27.909341347931132 22.552959178885228 72-73 22.335148380164274 27.264286468895122 27.76939241123247 22.631172739708138 74-75 22.29050122669859 27.341958471373886 27.742273687415338 22.625266614512185 76-77 22.354167970946502 27.351752454230223 27.637311052056248 22.65676852276703 78-79 22.30655853957002 27.297407046420822 27.63712017755453 22.758914236454622 80-81 22.25312351990631 27.38447743550311 27.62476052384529 22.73763852074529 82-83 22.386062264710294 27.294098255951702 27.579725223972336 22.740114255365665 84-85 22.318719724434843 27.28654481893833 27.523896551361048 22.87083890526578 86-87 22.355796963443016 27.250807575460694 27.565196610746796 22.82819885034949 88-89 22.38282861253933 27.25072252846737 27.390043596632946 22.976405262360352 90-91 22.301893719943884 27.20607237434085 27.534281913876956 22.95775199183831 92-93 22.371960282033363 27.188962032896264 27.523317561299088 22.91576012377129 94-95 22.32007989556626 27.186100771785128 27.447848938231783 23.04597039441683 96-97 22.352559719697 27.12077342898953 27.482139427715467 23.044527423597998 98-99 22.39717508673346 27.175254975707308 27.298151933280845 23.129418004278392 100-101 22.319531335302052 27.307025232181807 27.245782294690805 23.127661137825335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2208.0 1 1378.5 2 593.5 3 595.0 4 466.5 5 450.0 6 710.5 7 1339.5 8 2166.5 9 2484.0 10 1990.5 11 1525.5 12 1534.0 13 1713.5 14 1904.5 15 2130.0 16 2381.0 17 2609.0 18 2914.0 19 3247.5 20 3625.5 21 4167.5 22 4887.5 23 5943.5 24 7403.0 25 9605.0 26 13561.0 27 18868.0 28 25903.0 29 35360.5 30 46394.5 31 58921.0 32 73038.5 33 86417.0 34 97772.5 35 107887.5 36 119044.5 37 129757.5 38 137187.0 39 144259.0 40 151098.0 41 157333.0 42 163511.5 43 169440.0 44 174695.0 45 179760.5 46 183314.5 47 184568.5 48 183890.0 49 179632.5 50 171671.5 51 162410.0 52 152983.5 53 143969.5 54 133154.0 55 119509.5 56 104710.5 57 90651.0 58 80771.5 59 71269.5 60 59419.5 61 48068.5 62 38240.0 63 28620.5 64 20205.0 65 14386.5 66 10253.0 67 7469.5 68 5787.0 69 4395.5 70 3092.0 71 2098.0 72 1475.0 73 1072.0 74 807.5 75 600.0 76 415.5 77 263.5 78 158.0 79 91.5 80 58.5 81 46.5 82 34.0 83 17.0 84 11.5 85 13.5 86 7.5 87 2.0 88 2.0 89 2.0 90 1.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018495191822378406 2 0.0 3 0.0018083170222374925 4 0.018678312533491063 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2890088889082184E-5 12-13 2.2890088889082184E-5 14-15 8.011531111178763E-5 16-17 0.001373405333344931 18-19 1.6023062222357527E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.5479637778077384E-4 28-29 0.005367725844489772 30-31 6.867026666724654E-5 32-33 2.1745584444628072E-4 34-35 0.011136028244538482 36-37 0.00408588086670117 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.0601080000173965E-4 60-61 0.002334789066686383 62-63 0.010300540000086983 64-65 0.002254673755574595 66-67 0.0048526988444854225 68-69 0.0032275025333605876 70-71 1.8312071111265747E-4 72-73 0.007153152777838182 74-75 0.006191769044496731 76-77 0.0016595314444584584 78-79 3.891315111143971E-4 80-81 0.007187487911171806 82-83 0.009568057155636352 84-85 0.001854097200015657 86-87 0.001625196311124835 88-89 3.891315111143971E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 4.578017777816437E-5 98-99 5.722522222270546E-5 100-101 8.240432000069586E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4368703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.01627586071474 #Duplication Level Percentage of deduplicated Percentage of total 1 56.79471538352385 24.998918597519655 2 20.508577262904918 18.0542238862961 3 9.511322810522545 12.559590258848072 4 4.702027717746378 8.278629965162061 5 2.644576456660562 5.820220342556141 6 1.6204022853694409 4.279444439889236 7 1.0279904036606926 3.1673816432787585 8 0.7090506368468728 2.496781474453394 9 0.48489037535842133 1.9208761671583612 >10 1.902582904188893 13.837212964600774 >50 0.07243689520731235 2.149688195362023 >100 0.02043810385281703 1.4491121398257896 >500 4.683620534758228E-4 0.1363375194683899 >1k 4.1632168309488436E-4 0.42715411946318854 >5k 1.0408042077371395E-4 0.4244282861182518 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9925 0.2271841322241407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8604 0.1969463248016631 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 4529 0.1036692125786532 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.156035555632873E-5 0.0 3 0.0 0.0 0.0 9.156035555632873E-5 0.0 4 0.0 0.0 0.0 9.156035555632873E-5 0.0 5 0.0 1.1445044444541091E-4 0.0 1.1445044444541091E-4 0.0 6 0.0 1.1445044444541091E-4 0.0 1.1445044444541091E-4 0.0 7 0.0 1.1445044444541091E-4 0.0 1.1445044444541091E-4 0.0 8 0.0 1.1445044444541091E-4 0.0 1.1445044444541091E-4 0.0 9 0.0 1.1445044444541091E-4 0.0 1.373405333344931E-4 0.0 10-11 0.0 1.373405333344931E-4 0.0 1.7167566666811637E-4 0.0 12-13 0.0 1.373405333344931E-4 0.0 1.8312071111265747E-4 0.0 14-15 0.0 1.6023062222357527E-4 0.0 1.8312071111265747E-4 1.1445044444541092E-5 16-17 0.0 1.8312071111265747E-4 0.0 1.8312071111265747E-4 2.2890088889082184E-5 18-19 0.0 1.8312071111265747E-4 0.0 2.1745584444628075E-4 2.2890088889082184E-5 20-21 0.0 2.2890088889082182E-4 0.0 3.090162000026095E-4 3.433513333362328E-5 22-23 0.0 2.2890088889082182E-4 0.0 4.463567333371026E-4 4.578017777816437E-5 24-25 0.0 2.2890088889082182E-4 0.0 5.722522222270546E-4 4.578017777816437E-5 26-27 0.0 2.7468106666898623E-4 0.0 8.011531111178764E-4 9.156035555632873E-5 28-29 0.0 2.975711555580684E-4 0.0 0.0017739818889038693 9.156035555632873E-5 30-31 0.0 3.204612444471506E-4 0.0 0.003868425022254889 1.1445044444541091E-4 32-33 0.0 3.204612444471506E-4 0.0 0.00692425188894736 1.373405333344931E-4 34-35 0.0 3.204612444471506E-4 0.0 0.009774067955638094 1.373405333344931E-4 36-37 0.0 3.319062888916917E-4 0.0 0.013905729000117426 1.373405333344931E-4 38-39 0.0 3.4335133333623273E-4 0.0 0.01889576837793734 1.373405333344931E-4 40-41 0.0 3.4335133333623273E-4 0.0 0.02432071944464982 1.373405333344931E-4 42-43 0.0 3.891315111143971E-4 0.0 0.0310275154891509 1.373405333344931E-4 44-45 0.0 4.120216000034793E-4 0.0 0.0350103909558512 1.373405333344931E-4 46-47 0.0 4.349116888925615E-4 0.0 0.03992031502255933 1.373405333344931E-4 48-49 0.0 4.349116888925615E-4 0.0 0.05027808024486902 1.373405333344931E-4 50-51 0.0 4.349116888925615E-4 0.0 0.0630850849783105 1.602306222235753E-4 52-53 0.0 4.349116888925615E-4 0.0 0.08006953093400948 1.602306222235753E-4 54-55 0.0 5.951423111161368E-4 0.0 0.09700819671193028 1.602306222235753E-4 56-57 0.0 6.638125777833833E-4 0.0 0.12437329797882804 1.602306222235753E-4 58-59 0.0 8.240432000069586E-4 0.0 0.1544737648679711 1.602306222235753E-4 60-61 0.0 8.69823377785123E-4 0.0 0.17775298526816768 1.602306222235753E-4 62-63 0.0 9.041585111187462E-4 0.0 0.1953211284905383 2.0601080000173965E-4 64-65 0.0 0.001007163911119616 0.0 0.23775935329089665 2.0601080000173965E-4 66-67 0.0 0.0010300540000086982 0.0 0.3116371151804094 2.0601080000173965E-4 68-69 0.0 0.0010300540000086982 0.0 0.40245354284784296 2.0601080000173965E-4 70-71 0.0 0.0010529440888977803 0.0 0.4936018768041682 2.0601080000173965E-4 72-73 0.0 0.0010758341777868626 0.0 0.5879433781605203 2.1745584444628072E-4 74-75 0.0 0.0010758341777868626 0.0 0.6950918842503142 2.2890088889082182E-4 76-77 0.0 0.0010758341777868626 0.0 0.809267647629056 2.2890088889082182E-4 78-79 0.0 0.0011559494888986502 0.0 0.9371774643412474 2.2890088889082182E-4 80-81 0.0 0.0011902846222322735 0.0 1.0761317489424207 2.2890088889082182E-4 82-83 0.0 0.0011902846222322735 0.0 1.2259473807214636 2.51790977779904E-4 84-85 0.0 0.0011902846222322735 0.0 1.3877345289894965 2.632360222244451E-4 86-87 0.0 0.0012131747111213556 0.0 1.5873933293245157 2.746810666689862E-4 88-89 0.0 0.0012131747111213556 0.0 1.8262971870598665 2.746810666689862E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6320 0.0 54.72888 1 GTATCAA 11000 0.0 40.12595 1 TCAACGC 13120 0.0 32.80402 4 ATCAACG 13235 0.0 32.555622 3 CAACGCA 13405 0.0 32.06564 5 TATCAAC 13540 0.0 31.927534 2 AACGCAG 14065 0.0 30.662266 6 GTGGTAT 3085 0.0 29.877817 1 ACGCAGA 15795 0.0 27.183598 7 CGCAGAG 16275 0.0 26.119219 8 TGGTATC 3455 0.0 24.887035 2 GCAGAGT 18495 0.0 22.444775 9 GAGTACT 11705 0.0 21.770897 12-13 CAGAGTA 17525 0.0 20.679714 10-11 GTACTTT 12745 0.0 19.565794 14-15 AGAGTAC 15900 0.0 19.402607 10-11 GTACATG 9540 0.0 19.323492 1 TACATGG 9780 0.0 17.826654 2 AGTACTT 12910 0.0 17.310572 12-13 ACATGGG 9700 0.0 16.945211 3 >>END_MODULE