FastQCFastQC Report
Fri 10 Feb 2017
SRR2031685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992998
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8900.041.6349141
GTATCAA16600.036.9177061
TCAACGC16350.036.02944
ATCAACG17200.034.5250783
CAACGCA17400.033.30585
TATCAAC19000.031.7543472
AACGCAG18500.031.5758516
ACGCAGA20550.028.4273827
CGCAGAG22050.026.2794768
CGCCGTA1500.023.75205444-45
ACCCGTA1000.00165459523.7472657
GTATAGG2709.986252E-1021.1140021
GCAGAGT27000.020.5829
CCGTATC1803.6379788E-1219.79138446-47
GTATAGA3900.019.489851
GCCGTAT1950.019.486946-47
TACATGG19150.019.1018332
GAGTACT12900.018.96720912-13
ACATGGG18450.018.7966123
GTACATG19600.018.663271
Produced by FastQC (version 0.11.5)