##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031685_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992998 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9006100717222 33.0 31.0 34.0 30.0 34.0 2 32.21350496174212 34.0 31.0 34.0 31.0 34.0 3 32.32546289116393 34.0 31.0 34.0 31.0 34.0 4 35.724612738394235 37.0 37.0 37.0 35.0 37.0 5 35.72792996561927 37.0 37.0 37.0 35.0 37.0 6 35.756127404083394 37.0 37.0 37.0 35.0 37.0 7 35.73574267017658 37.0 37.0 37.0 35.0 37.0 8 35.722590579235806 37.0 37.0 37.0 35.0 37.0 9 37.50735751733639 39.0 39.0 39.0 35.0 39.0 10-11 37.44525517674759 39.0 38.0 39.0 35.0 39.0 12-13 37.40348721749692 39.0 38.0 39.0 35.0 39.0 14-15 38.8383350218228 41.0 39.0 41.0 36.0 41.0 16-17 38.77176338723744 41.0 39.0 41.0 36.0 41.0 18-19 38.76468331255451 41.0 39.0 41.0 36.0 41.0 20-21 38.76528200459619 41.0 39.0 41.0 36.0 41.0 22-23 38.66173899645317 41.0 39.0 41.0 35.0 41.0 24-25 38.607534456262755 41.0 39.0 41.0 35.0 41.0 26-27 38.49783030781532 41.0 39.0 41.0 35.0 41.0 28-29 38.39354359223282 40.0 39.0 41.0 35.0 41.0 30-31 38.26477092602402 40.0 38.0 41.0 34.0 41.0 32-33 38.10852388423743 40.0 38.0 41.0 34.0 41.0 34-35 37.971036195440476 40.0 38.0 41.0 34.0 41.0 36-37 37.824350602921655 40.0 38.0 41.0 33.5 41.0 38-39 37.65845147724366 40.0 38.0 41.0 33.0 41.0 40-41 37.58506865069215 40.0 38.0 41.0 33.0 41.0 42-43 37.596487606218744 40.0 38.0 41.0 33.0 41.0 44-45 37.494110260040806 40.0 38.0 41.0 33.0 41.0 46-47 37.491894243492936 40.0 38.0 41.0 33.0 41.0 48-49 37.58831538432102 40.0 38.0 41.0 33.0 41.0 50-51 37.06753890742982 39.5 37.5 40.5 32.0 41.0 52-53 37.11725099144208 40.0 37.5 40.5 32.5 41.0 54-55 37.359315930142856 40.0 38.0 41.0 33.0 41.0 56-57 37.257013609292265 40.0 37.0 41.0 33.0 41.0 58-59 37.103905043111865 40.0 37.0 41.0 32.0 41.0 60-61 36.90219919878993 40.0 36.5 41.0 32.0 41.0 62-63 36.644596464444035 39.5 36.0 41.0 31.0 41.0 64-65 36.3955944523554 39.0 35.5 41.0 31.0 41.0 66-67 36.13372534486474 39.0 35.0 41.0 31.0 41.0 68-69 35.819737804104335 38.5 35.0 41.0 30.5 41.0 70-71 35.48356592863228 37.5 35.0 40.0 30.0 41.0 72-73 35.137075804785106 37.0 35.0 39.5 30.0 41.0 74-75 34.74409011901333 36.5 35.0 39.0 29.0 41.0 76-77 34.36380586869259 36.0 35.0 39.0 29.0 41.0 78-79 33.96900950455087 36.0 34.0 38.5 29.0 39.5 80-81 33.60404603030419 35.0 34.0 37.0 29.0 39.0 82-83 33.24848841588805 35.0 34.0 37.0 28.5 39.0 84-85 32.89997361525401 35.0 34.0 36.0 27.5 38.0 86-87 32.604961943528586 35.0 34.0 36.0 27.0 37.0 88-89 32.3218470732066 35.0 34.0 36.0 26.5 37.0 90-91 32.079928156955 35.0 34.0 35.0 26.0 36.0 92-93 31.86772178795929 35.0 34.0 35.0 25.5 36.0 94-95 31.630599960926407 35.0 33.5 35.0 25.0 36.0 96-97 31.39124197631818 35.0 33.0 35.0 24.5 36.0 98-99 31.163775254330822 35.0 33.0 35.0 23.5 35.0 100-101 30.58941911262661 34.5 32.0 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3408640563393526 1101 2 -0.07569465863036129 1101 3 -0.10399254481059472 1101 4 -0.07891331175047611 1101 5 -0.11957580289519854 1101 6 -0.05266700596609297 1101 7 -0.0339809895268246 1101 8 0.019034399213829545 1101 9 0.034710891517647724 1101 10-11 -0.16944600796968956 1101 12-13 -0.16818170387235654 1101 14-15 -0.23483589768414248 1101 16-17 -0.19219730298816984 1101 18-19 -0.30291028224377214 1101 20-21 -0.20047116721786296 1101 22-23 -0.21000412645809519 1101 24-25 -0.5084893621086266 1101 26-27 -0.6666294125895078 1101 28-29 -0.524101064522533 1101 30-31 -0.4396394467503626 1101 32-33 -0.6572626873975338 1101 34-35 -0.5596339096027805 1101 36-37 -0.5943874618954439 1101 38-39 -0.5133183380560737 1101 40-41 -0.6293208958312206 1101 42-43 -0.48276479038158726 1101 44-45 -0.7024313578066952 1101 46-47 -0.7192753238048297 1101 48-49 -0.5871453832153009 1101 50-51 -0.5954973702448356 1101 52-53 -0.6709968384100691 1101 54-55 -0.4025169389808809 1101 56-57 -0.4669983184109938 1101 58-59 -0.5694074754480809 1101 60-61 -0.6580928635173464 1101 62-63 -0.5128499988252031 1101 64-65 -0.3246871344363882 1101 66-67 -0.630799835637319 1101 68-69 -0.8691786235135268 1101 70-71 -0.7903565692429595 1101 72-73 -0.6491048401910149 1101 74-75 -0.38091963845997157 1101 76-77 -0.6985405974169936 1101 78-79 -0.9227556581726546 1101 80-81 -0.8242954204346162 1101 82-83 -0.6246595838134468 1101 84-85 -0.7935211194284832 1101 86-87 -0.9432197607288977 1101 88-89 -0.9057653877948191 1101 90-91 -1.3525825895852641 1101 92-93 -1.0930754799460694 1101 94-95 -1.1473953781693034 1101 96-97 -1.3000180156229035 1101 98-99 -1.0975524167444028 1101 100-101 -1.067650832805878 1102 1 0.055630387468642795 1102 2 0.17768488083578404 1102 3 0.2607170439166353 1102 4 0.3103065301558132 1102 5 0.26172216938112314 1102 6 0.16485504696093756 1102 7 0.12519531932871786 1102 8 -0.007847259624519154 1102 9 0.052273040675629545 1102 10-11 0.1755820593919779 1102 12-13 0.2665989124639907 1102 14-15 0.3533643543493028 1102 16-17 0.30350094423373974 1102 18-19 0.18088797643420662 1102 20-21 0.02084857158382647 1102 22-23 0.3072744850667064 1102 24-25 0.055535726675891794 1102 26-27 0.11901327271235829 1102 28-29 0.15280637046429035 1102 30-31 0.03888902157362395 1102 32-33 0.1633668234284542 1102 34-35 0.06025382095054965 1102 36-37 0.01662181751800773 1102 38-39 0.0809908611649135 1102 40-41 0.1972543132419915 1102 42-43 0.173725071000014 1102 44-45 -0.06199430888929669 1102 46-47 -0.052119864989386144 1102 48-49 0.19707674990419122 1102 50-51 -0.05938212222100958 1102 52-53 -0.13737328473840904 1102 54-55 -0.0076463925265315424 1102 56-57 0.10544984289041537 1102 58-59 -0.07226576391182249 1102 60-61 -0.19832083923049026 1102 62-63 -0.11087955547544937 1102 64-65 0.07073330318532811 1102 66-67 -0.019693179662979787 1102 68-69 -0.03996851926331857 1102 70-71 -0.14254181382996478 1102 72-73 -0.11259149387414169 1102 74-75 -0.26212023417540564 1102 76-77 0.22924036260133818 1102 78-79 0.012927123360178427 1102 80-81 0.012283798261684353 1102 82-83 0.08805378541556053 1102 84-85 0.04990596271151304 1102 86-87 0.10135292800978846 1102 88-89 0.08035845227849592 1102 90-91 -0.045986759593283466 1102 92-93 0.02089519252500338 1102 94-95 -0.07291376515521364 1102 96-97 -0.25744154638244154 1102 98-99 -0.08705006825087835 1102 100-101 -0.3912331446399939 1103 1 -0.2478959754523089 1103 2 -0.09045573950016461 1103 3 -0.06389965576586576 1103 4 -0.08278320960701535 1103 5 0.015469693193232104 1103 6 -0.09912015045568978 1103 7 0.018555065332897414 1103 8 0.11137881293795715 1103 9 0.09513619228616932 1103 10-11 0.0033852397670059986 1103 12-13 0.041464587750958515 1103 14-15 0.05132099053285799 1103 16-17 0.10546222072555622 1103 18-19 0.09670301537479986 1103 20-21 -0.19047812240005868 1103 22-23 -0.08657982010818444 1103 24-25 -0.11070467385598448 1103 26-27 0.012723755467241915 1103 28-29 -0.028943107161829573 1103 30-31 -0.0655497996252592 1103 32-33 -0.1203154375685358 1103 34-35 -0.19451199273157727 1103 36-37 -0.36480609488599924 1103 38-39 -0.3853947186022566 1103 40-41 -0.1808582210175942 1103 42-43 -0.15832494121132612 1103 44-45 -0.21904906995412432 1103 46-47 -0.2389438317745558 1103 48-49 -0.17984043009092687 1103 50-51 -0.1514420391839124 1103 52-53 -0.09414854185489929 1103 54-55 -0.27814922827047184 1103 56-57 -0.38031648638150983 1103 58-59 -0.3053958010885651 1103 60-61 -0.2744829515205254 1103 62-63 -0.2179682369223599 1103 64-65 -0.17252671689553978 1103 66-67 -0.41009710357277385 1103 68-69 -0.3661659087507232 1103 70-71 -0.3885690586808508 1103 72-73 -0.37777154326221307 1103 74-75 -0.3769441195516521 1103 76-77 -0.39454596612786474 1103 78-79 -0.3750012910654803 1103 80-81 -0.5140655545374244 1103 82-83 -0.35755759555103594 1103 84-85 -0.5113807052215193 1103 86-87 -0.30906809177258054 1103 88-89 -0.17359666427193332 1103 90-91 -0.3491121835980593 1103 92-93 -0.7681614138086097 1103 94-95 -0.3981383180845768 1103 96-97 -0.5252049720696697 1103 98-99 -0.6822971443139387 1103 100-101 -0.5694895013195982 1104 1 0.10074795139001225 1104 2 0.33895302936462457 1104 3 0.34353215377144863 1104 4 0.27623868999135226 1104 5 0.18501010462460243 1104 6 0.34872373512745014 1104 7 0.26537262884946244 1104 8 0.30414040580011914 1104 9 0.31525004183199457 1104 10-11 0.294466165777699 1104 12-13 0.328037303829781 1104 14-15 0.5199582127586595 1104 16-17 0.5281283372698411 1104 18-19 0.5413273956812787 1104 20-21 0.37068796513387525 1104 22-23 0.3746745878548339 1104 24-25 0.39257478616452346 1104 26-27 0.49564503424389983 1104 28-29 0.4957782322142563 1104 30-31 0.5942050950671316 1104 32-33 0.5868314257668885 1104 34-35 0.6017063456839367 1104 36-37 0.5224293467483818 1104 38-39 0.3841805052610141 1104 40-41 0.41674229987953737 1104 42-43 0.3963802168306714 1104 44-45 0.6567227667884623 1104 46-47 0.6098934974672474 1104 48-49 0.6901931574591202 1104 50-51 0.6676126098874349 1104 52-53 0.7657713895517091 1104 54-55 0.7971204069081281 1104 56-57 0.8319980890679872 1104 58-59 0.7298189078085855 1104 60-61 0.6564459602041737 1104 62-63 0.5389823229775885 1104 64-65 0.5852167928422745 1104 66-67 0.6174807892436078 1104 68-69 0.6089457811349845 1104 70-71 0.49301645419008366 1104 72-73 0.365186997710083 1104 74-75 0.43960467909711554 1104 76-77 0.4400395402963113 1104 78-79 0.40223073379316787 1104 80-81 0.3539643841540183 1104 82-83 0.3132850587034923 1104 84-85 0.4790110635723579 1104 86-87 0.3825266399460574 1104 88-89 0.5627816823954177 1104 90-91 0.5403970255795301 1104 92-93 0.3477710758604786 1104 94-95 0.4527896910955924 1104 96-97 0.5100709809206556 1104 98-99 0.4708517690869094 1104 100-101 0.2800215129470516 1105 1 -0.07015605640409817 1105 2 0.1127761974765562 1105 3 -0.0348597664962611 1105 4 0.0560272566134401 1105 5 0.14526861732936425 1105 6 0.17800489795051533 1105 7 0.10756369345948968 1105 8 0.1362318050485456 1105 9 0.11620615155466396 1105 10-11 0.19360022083267125 1105 12-13 0.06737516759020679 1105 14-15 0.15132405785678316 1105 16-17 0.24001677499865792 1105 18-19 0.12129145079676817 1105 20-21 0.26753340818180504 1105 22-23 0.12609755748322726 1105 24-25 0.17120028766827744 1105 26-27 0.19487167460746946 1105 28-29 -0.0681709850465424 1105 30-31 0.17733944058582551 1105 32-33 0.20211526327663876 1105 34-35 0.11126441724694303 1105 36-37 0.24639078769045142 1105 38-39 0.08672266788438776 1105 40-41 0.13162796004909438 1105 42-43 0.012185678758747542 1105 44-45 0.07896381536246366 1105 46-47 0.23420046920148962 1105 48-49 0.22626814550774554 1105 50-51 0.13574510975127652 1105 52-53 0.25882567600944384 1105 54-55 0.23569398802315078 1105 56-57 0.2952973937704755 1105 58-59 0.25120974488870473 1105 60-61 0.29633388962149354 1105 62-63 0.29221463948621107 1105 64-65 0.2104242020874878 1105 66-67 0.026322383647340075 1105 68-69 0.06215328720143276 1105 70-71 0.037196590102894334 1105 72-73 0.05416389979355074 1105 74-75 0.0903499000613266 1105 76-77 0.23763716333492368 1105 78-79 0.1122686756157023 1105 80-81 0.10823631870285766 1105 82-83 0.15143988796528873 1105 84-85 0.13632500038439588 1105 86-87 0.22940445759535777 1105 88-89 0.2413279115107727 1105 90-91 0.1786426247517099 1105 92-93 0.0689930809072834 1105 94-95 0.13520029786218402 1105 96-97 0.15350498817014469 1105 98-99 0.06892563158865173 1105 100-101 0.16027542664302175 1106 1 0.22765206619295242 1106 2 0.33734017409936 1106 3 0.3344735212491017 1106 4 0.2749909870612157 1106 5 0.3089585075291694 1106 6 0.30286916566821986 1106 7 0.3824461767147511 1106 8 0.4452581049835871 1106 9 0.38071697889376566 1106 10-11 0.364141275353127 1106 12-13 0.4007460323978975 1106 14-15 0.2775589121383817 1106 16-17 0.36813095399667617 1106 18-19 0.2948489747569738 1106 20-21 0.2172538085227771 1106 22-23 0.2510443551445647 1106 24-25 0.4562510494786025 1106 26-27 0.3989623302135499 1106 28-29 0.41433915139902666 1106 30-31 0.36191765951889465 1106 32-33 0.35944975831718295 1106 34-35 0.466808130410584 1106 36-37 0.5029787404408737 1106 38-39 0.47434518063821685 1106 40-41 0.5502964690984982 1106 42-43 0.4687065464549889 1106 44-45 0.27945498642699107 1106 46-47 0.3160041493295793 1106 48-49 0.40042931705566787 1106 50-51 0.31316149807588545 1106 52-53 0.44981411356435075 1106 54-55 0.427640362423773 1106 56-57 0.3653423254297863 1106 58-59 0.3617774683709527 1106 60-61 0.13131467782962147 1106 62-63 0.26891054140291004 1106 64-65 0.39785736757064427 1106 66-67 0.44828501644686725 1106 68-69 0.5151194723255301 1106 70-71 0.41821493886736505 1106 72-73 0.3243987682231335 1106 74-75 0.3280774026934452 1106 76-77 0.5252062674252329 1106 78-79 0.4176170875627392 1106 80-81 0.5912634462953292 1106 82-83 0.6773389632762203 1106 84-85 0.6408871377684591 1106 86-87 0.6318251787420124 1106 88-89 0.41212304035770586 1106 90-91 0.41515988471041965 1106 92-93 0.43595952776919944 1106 94-95 0.43838996228364024 1106 96-97 0.5718209764603266 1106 98-99 0.5614053852249761 1106 100-101 0.32309521543710673 1107 1 0.20900411822528042 1107 2 0.32347044694562044 1107 3 0.3837326569748356 1107 4 0.4574022829672586 1107 5 0.3672358717503741 1107 6 0.4164927803379399 1107 7 0.3892306943696582 1107 8 0.40272157976842493 1107 9 0.39690127519904905 1107 10-11 0.45860968249494505 1107 12-13 0.43795222614452456 1107 14-15 0.48332941603725743 1107 16-17 0.4915864339160194 1107 18-19 0.46740124207455835 1107 20-21 0.4455919933547605 1107 22-23 0.47090315631903223 1107 24-25 0.4768016945763165 1107 26-27 0.4589111873318572 1107 28-29 0.4992170048130191 1107 30-31 0.5197057046176212 1107 32-33 0.5886698041032048 1107 34-35 0.4216879294776774 1107 36-37 0.5265710477833139 1107 38-39 0.5483071620711257 1107 40-41 0.4584673277632234 1107 42-43 0.7353311415317378 1107 44-45 0.6988556747333732 1107 46-47 0.719255285136164 1107 48-49 0.8145868492600172 1107 50-51 0.8429407296187108 1107 52-53 0.7354157752026325 1107 54-55 0.7108326990773506 1107 56-57 0.767064102089023 1107 58-59 0.8063181426281929 1107 60-61 0.7810737453311418 1107 62-63 0.8164678623649237 1107 64-65 0.8601344386804683 1107 66-67 0.7116803327292018 1107 68-69 0.7223909086218114 1107 70-71 0.7096636671177379 1107 72-73 0.6739599855450109 1107 74-75 0.6196354160047406 1107 76-77 0.7082391615968646 1107 78-79 0.7026230508631741 1107 80-81 0.7718099474046056 1107 82-83 0.7024467322438142 1107 84-85 0.667205886283952 1107 86-87 0.7446054664965231 1107 88-89 0.7970578736127081 1107 90-91 0.7757159372076785 1107 92-93 0.7684413220151214 1107 94-95 0.9127123949059275 1107 96-97 0.8028925512264671 1107 98-99 0.9399397778021843 1107 100-101 0.8739759393211344 1108 1 -0.006658549853224116 1108 2 0.13697523122976918 1108 3 0.10857588040742883 1108 4 0.1522041183530689 1108 5 0.20860143554163812 1108 6 0.11376290986898852 1108 7 0.17921778478029893 1108 8 0.15946526881654677 1108 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11309.0 3 1040.0 4 826.0 5 711.0 6 838.0 7 842.0 8 988.0 9 1123.0 10 1249.0 11 1370.0 12 1527.0 13 1884.0 14 2165.0 15 2322.0 16 2501.0 17 2746.0 18 2680.0 19 2153.0 20 2206.0 21 2599.0 22 2717.0 23 2955.0 24 3366.0 25 3809.0 26 4586.0 27 5404.0 28 6683.0 29 8366.0 30 10619.0 31 13273.0 32 17877.0 33 24075.0 34 35234.0 35 55171.0 36 97245.0 37 195372.0 38 322969.0 39 140023.0 40 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22227579507778 22.141235307111998 15.072610832767625 22.5638780650426 2 14.954571085885714 22.57071063188911 40.52226859095215 21.952449691273028 3 17.21120987036671 27.113752909991 32.507978519525686 23.167058700116602 4 12.694907834240835 19.311838412856606 39.77088179340425 28.22237195949831 5 12.97083367793393 38.342145022723464 36.26667446640699 12.420346832935614 6 28.402056606075636 39.2418911983189 19.27014482757925 13.08590736802621 7 24.318450886311336 33.85005415282593 24.88831609922169 16.94317886164104 8 22.83246785984371 36.373884547517015 22.894580287370808 17.899067305268463 9 24.20509966313467 17.50822501918247 23.558794141104325 34.727881176578535 10-11 22.543857261387362 27.704804160857172 31.02284378336363 18.728494794391835 12-13 22.919830355684358 25.979810874418437 30.322459318236362 20.777899451660847 14-15 20.961784104369944 28.46527750812774 27.396051382157445 23.17688700534487 16-17 20.531494037688347 30.89331789379399 28.529827697161775 20.045360371355887 18-19 20.511140731640527 30.23563450552964 29.75385541785876 19.499369344971072 20-21 21.150960142004198 29.493051073099885 29.527442714216555 19.828546070679362 22-23 21.082121443305933 29.436460422237428 29.036802697866122 20.444615436590517 24-25 20.986768008082578 29.47780871034713 28.97269811436805 20.562725167202245 26-27 20.83782261711674 29.37602442640944 29.184033493544252 20.602119462929565 28-29 21.07214939624664 29.29669754550776 29.20382404858867 20.427329009656926 30-31 21.312302692805236 29.191886193764617 28.96325939638492 20.532551717045227 32-33 21.06361386525454 29.40112735729442 28.711716258053006 20.823542519398035 34-35 21.33746801295869 29.403975847420238 28.699870160975177 20.558685978645894 36-37 21.329167635228455 29.07182955060582 28.872856284928105 20.726146529237624 38-39 21.365825327299568 29.125073607111457 28.83407343771427 20.675027627874712 40-41 21.41819621011024 28.99464817349699 28.95239823840963 20.634757377983146 42-43 21.370609148418296 29.152895103541315 28.591882457566108 20.88461329047428 44-45 21.402667656330994 29.358741889223484 28.416207635878827 20.822382818566695 46-47 21.418742540543892 29.0249548587878 28.5100349381133 21.046267662555003 48-49 21.31581906857423 29.09652799385955 28.59474198869645 20.992910948869774 50-51 21.297280017103574 29.198607712442094 28.481286156641932 21.0228261138124 52-53 21.293997304844474 29.239664663365193 28.538047015490843 20.92829101629949 54-55 21.39127848506464 29.144509165883203 28.40755854545638 21.05665380359578 56-57 21.224671518198793 29.30195316773988 28.449329926874068 21.024045387187257 58-59 21.23278554964235 29.111654704694917 28.54599023548178 21.109569510180954 60-61 21.369427233265945 29.0869308757619 28.62971570886248 20.913926182109673 62-63 21.165721960424683 29.215294712552346 28.56200803727132 21.056975289751648 64-65 21.13990295736962 29.270425396082594 28.4136121905392 21.176059456008584 66-67 21.31649445082281 29.113326647395432 28.483406419811352 21.086772481970407 68-69 21.180704556101215 28.991019710849713 28.62301392602872 21.20526180702035 70-71 21.1492299896895 29.014654018447978 28.71839730969061 21.11771868217191 72-73 21.140252338446523 28.846285711980833 28.650255524945443 21.363206424627197 74-75 21.054441006532034 29.119924668382595 28.570917277375006 21.25471704771036 76-77 21.13231503406657 29.10664509856916 28.606235654324173 21.154804213040094 78-79 20.98787033792114 29.144116319702697 28.50372260362907 21.364290738747098 80-81 21.044860937497635 29.03896936878427 28.552829228715797 21.3633404650023 82-83 21.14975660750243 29.075078252213427 28.385873805036887 21.389291335247258 84-85 21.067007252749026 29.02455971214896 28.518067331972603 21.39036570312941 86-87 20.84629729176395 28.899903280885823 28.722349578581003 21.531449848769228 88-89 21.072932354710332 28.881815491211505 28.52398313745915 21.52126901661901 90-91 20.9689513028351 28.87259066900299 28.460070341749972 21.69838768641194 92-93 20.967497904505993 28.75592714640619 28.693642306485796 21.582932642602024 94-95 21.009000342346784 28.52227699434649 28.72415571686358 21.744566946443147 96-97 21.02301914052398 28.422637126536966 28.77919359660271 21.775150136336347 98-99 20.922683548872847 28.469969404530186 28.767908482794397 21.839438563802567 100-101 20.819961112750327 28.503244386945788 28.798404160078277 21.878390340225607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1829.0 1 1048.5 2 244.5 3 206.5 4 175.0 5 164.0 6 203.0 7 288.5 8 415.0 9 450.5 10 329.5 11 242.0 12 229.5 13 233.5 14 242.5 15 250.0 16 292.5 17 311.0 18 336.5 19 388.0 20 425.5 21 521.5 22 600.5 23 661.5 24 806.5 25 1074.5 26 1757.5 27 3205.5 28 5783.0 29 10384.5 30 17054.0 31 24649.0 32 32966.5 33 40238.0 34 45435.5 35 48704.0 36 50299.5 37 51169.5 38 49418.5 39 44999.5 40 40522.0 41 36527.5 42 33203.0 43 30788.5 44 29189.5 45 28595.0 46 28076.0 47 27246.0 48 26871.5 49 26491.5 50 25624.5 51 24509.0 52 23456.0 53 22762.5 54 21615.5 55 19936.0 56 17920.5 57 15838.0 58 14282.0 59 12584.0 60 10502.5 61 8562.5 62 6945.0 63 5279.5 64 3936.5 65 3140.5 66 2459.0 67 1930.5 68 1510.0 69 1189.5 70 929.5 71 744.0 72 595.0 73 443.5 74 356.5 75 272.5 76 206.5 77 181.5 78 156.5 79 128.5 80 88.0 81 64.5 82 50.5 83 42.0 84 36.0 85 24.0 86 15.0 87 9.5 88 9.5 89 8.0 90 4.5 91 3.0 92 3.0 93 3.0 94 2.5 95 2.0 96 1.5 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16284020713032654 2 0.1680768742736642 3 0.15971834787179834 4 0.16435078419090474 5 0.15226616770627938 6 0.12759340905016928 7 0.13695898682575391 8 0.1256800114401036 9 0.12155110080785661 10-11 0.10946648432323128 12-13 0.1284997552865162 14-15 0.1496981867032965 16-17 0.16153104034449214 18-19 0.15795600796779047 20-21 0.14884219303563553 22-23 0.1409368397519431 24-25 0.1243708446542692 26-27 0.12935574895417715 28-29 0.133585364723796 30-31 0.12155110080785661 32-33 0.12180286365128631 34-35 0.1395269678287368 36-37 0.14174248085091812 38-39 0.12547860116535986 40-41 0.12895292840468964 42-43 0.12286026759369102 44-45 0.14259847451857907 46-47 0.13972837810348057 48-49 0.155035558984006 50-51 0.14023190379033995 52-53 0.1227092098876332 54-55 0.11485420917262672 56-57 0.13620369829546486 58-59 0.1252771908906161 60-61 0.12416943437952545 62-63 0.12522683832193016 64-65 0.1485400776235199 66-67 0.1374121599439274 68-69 0.14476363497207448 70-71 0.12995997977840842 72-73 0.13232655050664754 74-75 0.1124876384443876 76-77 0.14199424369434782 78-79 0.14305164763675254 80-81 0.14214530140040565 82-83 0.15030241752752777 84-85 0.1383688587489602 86-87 0.14773443652454488 88-89 0.13418959554802729 90-91 0.14204459626303376 92-93 0.15961764273442644 94-95 0.1322761979379616 96-97 0.11576055540897362 98-99 0.11928523521698936 100-101 0.16822793197972202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 992998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72122925702548 #Duplication Level Percentage of deduplicated Percentage of total 1 61.763699618522004 28.856759696390238 2 18.618589692292016 17.397667949121338 3 8.063128960126802 11.301578900251371 4 3.877250994083084 7.245997303263428 5 2.154205328791572 5.032356051658849 6 1.371364923160505 3.8443112982015055 7 0.8785836914633423 2.8733957049239787 8 0.6474694484906509 2.420045483188124 9 0.46861034596245116 1.9704646265333132 >10 2.081512101178926 16.024315599634303 >50 0.06299050581384529 1.9340708900026513 >100 0.01172581165668249 0.8173025344842224 >500 8.685784581905987E-4 0.28173396234666864 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0070513737187789E-4 0.0 0.0 0.0 6 0.0 1.0070513737187789E-4 0.0 0.0 0.0 7 0.0 1.0070513737187789E-4 0.0 0.0 0.0 8 0.0 1.0070513737187789E-4 0.0 0.0 0.0 9 0.0 1.0070513737187789E-4 0.0 0.0 0.0 10-11 0.0 1.0070513737187789E-4 0.0 0.0 0.0 12-13 0.0 1.0070513737187789E-4 0.0 0.0 0.0 14-15 0.0 1.0070513737187789E-4 0.0 0.0 0.0 16-17 0.0 1.0070513737187789E-4 0.0 0.0 0.0 18-19 0.0 1.0070513737187789E-4 0.0 0.0 0.0 20-21 0.0 1.0070513737187789E-4 0.0 1.5105770605781683E-4 0.0 22-23 0.0 1.0070513737187789E-4 0.0 5.035256868593895E-4 0.0 24-25 0.0 1.5105770605781683E-4 0.0 9.06346236346901E-4 0.0 26-27 0.0 2.0141027474375578E-4 0.0 0.0014098719232062905 0.0 28-29 0.0 2.5176284342969473E-4 0.0 0.0037764426514454207 0.0 30-31 0.0 3.0211541211563366E-4 0.0 0.009164167500840889 0.0 32-33 0.0 3.0211541211563366E-4 0.0 0.017623399040078633 0.0 34-35 0.0 3.0211541211563366E-4 0.0 0.02512593177428353 0.0 36-37 0.0 3.0211541211563366E-4 0.0 0.03499503523672756 0.0 38-39 0.0 3.5246798080157263E-4 0.0 0.04763352997689824 0.0 40-41 0.0 4.0282054948751156E-4 0.0 0.058761447656490745 0.0 42-43 0.0 4.0282054948751156E-4 0.0 0.07492462220467716 0.0 44-45 0.0 4.0282054948751156E-4 0.0 0.08615324502164154 0.0 46-47 0.0 4.0282054948751156E-4 0.0 0.10025196425370445 0.0 48-49 0.0 4.0282054948751156E-4 0.0 0.12895292840468964 0.0 50-51 0.0 4.0282054948751156E-4 0.0 0.16087645695157493 0.0 52-53 0.0 4.0282054948751156E-4 0.0 0.1948140882458978 0.0 54-55 0.0 6.545833929172063E-4 0.0 0.22356540496556893 0.0 56-57 0.0 8.056410989750231E-4 0.0 0.28977903278757866 0.0 58-59 0.0 8.056410989750231E-4 0.0 0.36158179573372756 0.0 60-61 0.0 8.559936676609621E-4 0.0 0.42029289082153237 0.0 62-63 0.0 9.06346236346901E-4 0.0 0.46480456153990235 0.0 64-65 0.0 9.06346236346901E-4 0.0 0.5721058854096382 0.0 66-67 0.0 0.001007051373718779 0.0 0.7440095549034338 0.0 68-69 1.0070513737187789E-4 0.001007051373718779 0.0 0.9332848605938784 0.0 70-71 1.0070513737187789E-4 0.001007051373718779 0.0 1.1358028918487246 0.0 72-73 1.0070513737187789E-4 0.001007051373718779 0.0 1.3487439048215606 0.0 74-75 1.0070513737187789E-4 0.001007051373718779 0.0 1.5878682535110844 0.0 76-77 1.0070513737187789E-4 0.001007051373718779 0.0 1.8442635332598858 0.0 78-79 1.0070513737187789E-4 0.001007051373718779 0.0 2.132028463300027 0.0 80-81 1.0070513737187789E-4 0.001007051373718779 0.0 2.4367118564186434 0.0 82-83 1.0070513737187789E-4 0.001007051373718779 0.0 2.74945166052701 0.0 84-85 1.0070513737187789E-4 0.001007051373718779 0.0 3.0772468826724726 5.0352568685938945E-5 86-87 1.0070513737187789E-4 0.001007051373718779 0.0 3.447338262514124 1.0070513737187789E-4 88-89 1.0070513737187789E-4 0.001007051373718779 0.0 3.854589838045998 1.0070513737187789E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 890 0.0 41.634914 1 GTATCAA 1660 0.0 36.917706 1 TCAACGC 1635 0.0 36.0294 4 ATCAACG 1720 0.0 34.525078 3 CAACGCA 1740 0.0 33.3058 5 TATCAAC 1900 0.0 31.754347 2 AACGCAG 1850 0.0 31.575851 6 ACGCAGA 2055 0.0 28.427382 7 CGCAGAG 2205 0.0 26.279476 8 CGCCGTA 150 0.0 23.752054 44-45 ACCCGTA 100 0.001654595 23.747265 7 GTATAGG 270 9.986252E-10 21.114002 1 GCAGAGT 2700 0.0 20.582 9 CCGTATC 180 3.6379788E-12 19.791384 46-47 GTATAGA 390 0.0 19.48985 1 GCCGTAT 195 0.0 19.4869 46-47 TACATGG 1915 0.0 19.101833 2 GAGTACT 1290 0.0 18.967209 12-13 ACATGGG 1845 0.0 18.796612 3 GTACATG 1960 0.0 18.66327 1 >>END_MODULE