FastQCFastQC Report
Fri 10 Feb 2017
SRR2031685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992998
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT51640.5200413293883774No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT24530.24702970197321644RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT13480.1357505251772914RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.11772430558772526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.10201430415771232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.037.9206281
GTATCAA18150.036.3830031
TCAACGC18600.033.4560744
ACGCGTT300.00395012731.66914428-29
CAACGCA19650.031.6651555
ATCAACG19750.031.267483
AACGCAG20050.031.0334346
TATCAAC21100.029.9423472
GTGCGAG804.4891617E-429.6920641
ACGCAGA22750.027.3503467
AACGCGT350.00838981527.1449828-29
CGCAGAG22800.027.082048
CGGAGAT1457.8751873E-726.2109241
TGGACCG1457.889048E-726.2056455
TACCGTA950.00122677824.9988087
GCAGAGT26750.021.4849559
GTACATG15800.020.746861
GGACCGA1150.003723955720.651196
AGTACGT1150.003723955720.651195
GAGTACT17750.019.8028712-13
Produced by FastQC (version 0.11.5)