##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992998
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82469853917128	34.0	31.0	34.0	31.0	34.0
2	32.99229907814517	34.0	31.0	34.0	31.0	34.0
3	33.05918843743895	34.0	33.0	34.0	31.0	34.0
4	36.428414760150574	37.0	37.0	37.0	35.0	37.0
5	36.39890009848963	37.0	37.0	37.0	35.0	37.0
6	36.43104115013323	37.0	37.0	37.0	35.0	37.0
7	36.38011053395878	37.0	37.0	37.0	35.0	37.0
8	36.358704649959016	37.0	37.0	37.0	35.0	37.0
9	38.22433479221509	39.0	39.0	39.0	37.0	39.0
10-11	38.203328204085004	39.0	39.0	39.0	37.0	39.0
12-13	38.16058692968163	39.0	39.0	39.0	37.0	39.0
14-15	39.70094098880361	41.0	40.0	41.0	37.0	41.0
16-17	39.682229974279906	41.0	40.0	41.0	37.0	41.0
18-19	39.68164487743178	41.0	40.0	41.0	37.0	41.0
20-21	39.66775965309094	41.0	40.0	41.0	37.0	41.0
22-23	39.594927180115164	41.0	40.0	41.0	37.0	41.0
24-25	39.528884247501004	41.0	39.5	41.0	37.0	41.0
26-27	39.41355269597723	41.0	39.0	41.0	36.0	41.0
28-29	39.32701173617671	41.0	39.0	41.0	36.0	41.0
30-31	39.19434731993418	40.5	39.0	41.0	36.0	41.0
32-33	39.04144570281108	40.0	39.0	41.0	35.5	41.0
34-35	38.9227083035414	40.0	38.5	41.0	35.0	41.0
36-37	38.83478969746163	40.0	38.5	41.0	35.0	41.0
38-39	38.876591896459004	40.0	38.5	41.0	35.0	41.0
40-41	38.82292411465079	40.0	39.0	41.0	35.0	41.0
42-43	38.74854078255948	40.0	38.5	41.0	35.0	41.0
44-45	38.62290306727707	40.0	38.0	41.0	35.0	41.0
46-47	38.487643479644476	40.0	38.0	41.0	35.0	41.0
48-49	38.339067148171495	40.0	38.0	41.0	34.0	41.0
50-51	38.23087559088739	40.0	38.0	41.0	34.0	41.0
52-53	38.3158385011853	40.0	38.0	41.0	34.0	41.0
54-55	38.27139833111447	40.0	38.0	41.0	34.0	41.0
56-57	38.121088360701634	40.0	38.0	41.0	34.0	41.0
58-59	37.95545056485511	40.0	37.5	41.0	34.0	41.0
60-61	37.72886501281977	40.0	37.0	41.0	34.0	41.0
62-63	37.47489571983025	40.0	37.0	41.0	33.0	41.0
64-65	37.15148268173753	39.5	36.0	41.0	33.0	41.0
66-67	36.78335555560031	39.0	36.0	41.0	32.5	41.0
68-69	36.370643747520134	39.0	35.0	41.0	32.0	41.0
70-71	36.0331798251356	38.0	35.0	40.0	31.0	41.0
72-73	35.66662118151295	37.0	35.0	40.0	31.0	41.0
74-75	35.28142453459121	37.0	35.0	39.0	31.0	41.0
76-77	34.31336820416557	36.0	34.0	39.0	29.5	40.0
78-79	34.48744206936973	36.0	35.0	38.5	30.0	39.5
80-81	34.224782930076394	35.5	35.0	37.0	30.0	39.0
82-83	33.89628680017482	35.0	34.5	37.0	30.0	39.0
84-85	33.55043111869309	35.0	34.0	36.5	30.0	38.0
86-87	33.25492850942298	35.0	34.0	36.0	30.0	37.0
88-89	32.99155939891118	35.0	34.0	36.0	29.0	37.0
90-91	32.76208260238188	35.0	34.0	35.5	29.0	36.5
92-93	32.576812340004714	35.0	34.0	35.0	29.0	36.0
94-95	32.38690460605157	35.0	34.0	35.0	29.0	36.0
96-97	32.22136046598281	35.0	34.0	35.0	29.0	36.0
98-99	32.02909018950693	35.0	34.0	35.0	29.0	35.5
100-101	31.38087538947712	34.5	33.0	35.0	25.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10283438886251162
1101	2	0.09928696359470024
1101	3	0.08895602810974879
1101	4	0.07331581419839495
1101	5	0.033428342779593834
1101	6	-0.0018742475809645498
1101	7	0.049184065836684
1101	8	0.12600072123329653
1101	9	0.17944713544444113
1101	10-11	0.06764234281167347
1101	12-13	0.10955916792048015
1101	14-15	0.0644156703581018
1101	16-17	0.04502905553012937
1101	18-19	0.1001175747354921
1101	20-21	0.041489185978491605
1101	22-23	-0.07629020403581421
1101	24-25	-0.0034575486658781074
1101	26-27	0.11818819215890386
1101	28-29	0.14352969909322155
1101	30-31	0.11998746123187232
1101	32-33	-0.01800117235043075
1101	34-35	-0.33349414902429686
1101	36-37	-0.020843805509642266
1101	38-39	-0.12098709344058278
1101	40-41	-0.43432226804917207
1101	42-43	-0.2288015870407989
1101	44-45	-0.19919481032842157
1101	46-47	-0.24653531984380095
1101	48-49	-0.363388380601414
1101	50-51	-0.2699124234296164
1101	52-53	-0.17921317010797821
1101	54-55	-0.19828676546098478
1101	56-57	-0.536273528501745
1101	58-59	-0.41122065057111
1101	60-61	-0.5097738244442382
1101	62-63	-0.3790521787667416
1101	64-65	-0.26114185717698746
1101	66-67	-0.5008288179187304
1101	68-69	-0.5424960809740185
1101	70-71	-0.5262188172834144
1101	72-73	-0.6463478053797473
1101	74-75	-0.6495951637887529
1101	76-77	-0.6764127283749559
1101	78-79	-0.6159710965163328
1101	80-81	-0.4761459120512441
1101	82-83	-0.5120661107971713
1101	84-85	-0.7086292770925908
1101	86-87	-0.5920493597891365
1101	88-89	-0.5649937215237273
1101	90-91	-0.6683011984522977
1101	92-93	-0.7628746716152399
1101	94-95	-0.8203942660520944
1101	96-97	-0.8925245660343002
1101	98-99	-1.0375285046998712
1101	100-101	-1.0154475755350951
1102	1	0.11816833318602704
1102	2	0.04255079679438012
1102	3	0.09239857593883016
1102	4	-0.03804287522765293
1102	5	0.08515248071062587
1102	6	0.11298427000409816
1102	7	0.15699710283977453
1102	8	0.00780276041304262
1102	9	0.1018322883829228
1102	10-11	0.12481384011949359
1102	12-13	0.09867304121639364
1102	14-15	0.13926674150027196
1102	16-17	0.20867323012057426
1102	18-19	0.17475957267340192
1102	20-21	0.2061987747057188
1102	22-23	0.10950427414038444
1102	24-25	0.12224699939231698
1102	26-27	0.2331154461398839
1102	28-29	0.19551159850896482
1102	30-31	0.12905493746053764
1102	32-33	0.19217181424597385
1102	34-35	0.08148179323712412
1102	36-37	0.07355131420624872
1102	38-39	-0.030882270436798365
1102	40-41	0.0945298341389389
1102	42-43	0.136059794560758
1102	44-45	0.20040995539502404
1102	46-47	0.2021558246171935
1102	48-49	0.24704236747969333
1102	50-51	0.23333823529417685
1102	52-53	0.2883862112644593
1102	54-55	0.42360577664006627
1102	56-57	0.4100003860360104
1102	58-59	0.21327814654195265
1102	60-61	0.21737991596130257
1102	62-63	0.1443010117476362
1102	64-65	0.1933575127393823
1102	66-67	0.2763077383878354
1102	68-69	0.1535773524089521
1102	70-71	0.2628979200289905
1102	72-73	0.11547485471876939
1102	74-75	-0.09641896949386819
1102	76-77	0.035275322948216115
1102	78-79	0.14705445051082222
1102	80-81	-0.04088986897643565
1102	82-83	0.017098169189075918
1102	84-85	0.2386384511695212
1102	86-87	0.08922856437171589
1102	88-89	0.011767648621763271
1102	90-91	-0.044083074955914014
1102	92-93	-0.0506854174051341
1102	94-95	0.04422367414723993
1102	96-97	0.013982479446283946
1102	98-99	-0.02244945784455865
1102	100-101	0.15671132010013977
1103	1	0.14139972446442073
1103	2	0.010102559297436642
1103	3	0.06032481946003543
1103	4	0.029884725108836108
1103	5	-0.08112523224153279
1103	6	0.001748482644188698
1103	7	-0.003267204777387178
1103	8	0.051193713168729005
1103	9	-0.0451175908605137
1103	10-11	-0.08692939615631445
1103	12-13	0.008630708579552504
1103	14-15	0.004036354265188891
1103	16-17	0.0876202057793094
1103	18-19	0.09325364339061082
1103	20-21	0.18076527222274308
1103	22-23	0.1476558846801197
1103	24-25	0.03593048152306011
1103	26-27	0.05288492322328153
1103	28-29	0.06677696444204884
1103	30-31	-0.04410454260784746
1103	32-33	-0.19600354056122882
1103	34-35	-0.14003661874626516
1103	36-37	-0.31108614475009233
1103	38-39	-0.3329290262914597
1103	40-41	-0.3986460633943736
1103	42-43	-0.2825346804874158
1103	44-45	-0.306553220638186
1103	46-47	-0.5012307863296002
1103	48-49	-0.49469259222288997
1103	50-51	-0.5560535044143791
1103	52-53	-0.6574415817398034
1103	54-55	-0.589009213455185
1103	56-57	-0.4386631509100738
1103	58-59	-0.4201883808250102
1103	60-61	-0.5965749292260725
1103	62-63	-0.7775165780056952
1103	64-65	-0.6845270765526337
1103	66-67	-0.4765754940888911
1103	68-69	-0.7313199366741117
1103	70-71	-0.7651623768421913
1103	72-73	-0.6057620180933583
1103	74-75	-0.5175595084209021
1103	76-77	-0.4173494362170658
1103	78-79	-0.5000121148469674
1103	80-81	-0.443925743172521
1103	82-83	-0.4294582336222419
1103	84-85	-0.4989039493080796
1103	86-87	-0.5407814760613832
1103	88-89	-0.520855618046717
1103	90-91	-0.570108827309987
1103	92-93	-0.5552287133808207
1103	94-95	-0.5429313555670134
1103	96-97	-0.5032348280894716
1103	98-99	-0.5176904282388506
1103	100-101	-0.843091263276154
1104	1	0.2124892891156236
1104	2	0.14213322002449758
1104	3	0.12809817763483267
1104	4	0.05675347579380485
1104	5	0.13211380667747363
1104	6	0.09302410040368869
1104	7	0.1318011629708309
1104	8	0.14394563591091725
1104	9	0.048838584155760145
1104	10-11	0.16178544489914515
1104	12-13	0.21966109209650853
1104	14-15	0.3082022419499637
1104	16-17	0.1944595592995526
1104	18-19	0.3286366123753126
1104	20-21	0.31332327682572725
1104	22-23	0.3145441045399764
1104	24-25	0.3361683664744177
1104	26-27	0.36777110591118145
1104	28-29	0.2738361520230299
1104	30-31	0.2732152869401716
1104	32-33	0.32586704744653616
1104	34-35	0.10150299328851986
1104	36-37	0.09566682236506097
1104	38-39	0.13962717322245766
1104	40-41	0.04013885379716697
1104	42-43	0.013995680465932026
1104	44-45	0.018102361922068155
1104	46-47	0.08948718784473897
1104	48-49	-0.008755525363682182
1104	50-51	-0.01347854486771638
1104	52-53	-0.018115137829717298
1104	54-55	-0.025382402725220743
1104	56-57	0.031624438457953374
1104	58-59	0.22456235571579697
1104	60-61	0.22002028599856516
1104	62-63	0.15199727282947606
1104	64-65	0.21181761809721422
1104	66-67	0.22803586378935137
1104	68-69	0.2020355304893826
1104	70-71	0.29628478884563947
1104	72-73	0.18785054532650491
1104	74-75	0.27348338178455833
1104	76-77	0.17946458158884582
1104	78-79	0.32776240374222
1104	80-81	0.30278544902433424
1104	82-83	0.22426121685708011
1104	84-85	0.21721033861651762
1104	86-87	0.27019991218104167
1104	88-89	0.26356001660325745
1104	90-91	0.34548714218400534
1104	92-93	0.3044572011224247
1104	94-95	0.448508654232036
1104	96-97	0.4463896489182062
1104	98-99	0.4629899614025206
1104	100-101	0.5063933193292307
1105	1	0.08049289570672613
1105	2	0.027885369758543277
1105	3	0.004710402526363566
1105	4	0.040328963076937896
1105	5	0.04407909626375073
1105	6	0.05765046119592654
1105	7	0.07242795945863634
1105	8	0.07247582900877347
1105	9	-0.02280792150525457
1105	10-11	-0.05405087879845638
1105	12-13	0.09020746644372224
1105	14-15	0.023092743181415187
1105	16-17	0.09123964801201367
1105	18-19	0.1295170805059982
1105	20-21	0.12157486681198293
1105	22-23	0.021558198081393698
1105	24-25	0.05071817797557543
1105	26-27	0.08077903920135299
1105	28-29	0.2255429289045381
1105	30-31	0.0562461547610269
1105	32-33	-0.018374812005340857
1105	34-35	-0.08095420869873493
1105	36-37	-0.11716864809538663
1105	38-39	0.015399473876605896
1105	40-41	-0.08654265879261658
1105	42-43	-0.1085624576616695
1105	44-45	0.03311328456795337
1105	46-47	-0.004115348871692959
1105	48-49	0.04344382956482207
1105	50-51	0.0894763182815197
1105	52-53	0.10107120069363162
1105	54-55	0.043899849336767716
1105	56-57	0.023416418028674002
1105	58-59	0.13503175717900717
1105	60-61	0.21086039177576765
1105	62-63	0.09515049848952373
1105	64-65	0.11409352231680003
1105	66-67	0.10113771368012436
1105	68-69	-0.08632050540708747
1105	70-71	-0.00251765347007904
1105	72-73	-0.24859962501835753
1105	74-75	-0.09400041578643936
1105	76-77	-0.13114702606728912
1105	78-79	0.10083702265988137
1105	80-81	0.09977914959255685
1105	82-83	0.12773649415915145
1105	84-85	0.1456897005645672
1105	86-87	0.11923442290096631
1105	88-89	0.10734318544134425
1105	90-91	0.1270069935634055
1105	92-93	0.09347706763014685
1105	94-95	0.12132064554233324
1105	96-97	0.16441231719049654
1105	98-99	0.16113583469982729
1105	100-101	0.09703010050718319
1106	1	0.1806936792695879
1106	2	0.12176200164080342
1106	3	0.10230795586723218
1106	4	0.057844175114048824
1106	5	0.134129875759065
1106	6	0.1179020360441001
1106	7	0.12036606671691175
1106	8	0.10330429995293144
1106	9	0.035663364558857324
1106	10-11	0.16321096631170207
1106	12-13	0.07372623670040213
1106	14-15	0.17427875742136933
1106	16-17	0.0833515538995826
1106	18-19	0.16298709914597254
1106	20-21	0.19416407873355013
1106	22-23	0.18646186795218256
1106	24-25	0.2222307278325033
1106	26-27	0.1664026767204021
1106	28-29	0.11476711206442047
1106	30-31	0.15827615557453356
1106	32-33	0.19575030574720387
1106	34-35	-0.062383202882735134
1106	36-37	0.1129366247427086
1106	38-39	0.05915390249233354
1106	40-41	0.02401327469767267
1106	42-43	0.029402849108031148
1106	44-45	0.16677288627845144
1106	46-47	-0.035784446241699186
1106	48-49	-0.034617707119146246
1106	50-51	0.13183186435268368
1106	52-53	0.05987343183171134
1106	54-55	0.016344280157220226
1106	56-57	0.23993254614816095
1106	58-59	0.02625847047116281
1106	60-61	0.20431635674534476
1106	62-63	0.18730048605108607
1106	64-65	0.18096046164668422
1106	66-67	0.1805934169118757
1106	68-69	0.21785126534359023
1106	70-71	0.0676304406187711
1106	72-73	0.07004966634417542
1106	74-75	0.27936269120655766
1106	76-77	0.27821033684163154
1106	78-79	0.1910039335758853
1106	80-81	0.25592403444559864
1106	82-83	0.29435678685749167
1106	84-85	0.38578404408012545
1106	86-87	0.20562028339941918
1106	88-89	0.29706066186430746
1106	90-91	0.22286375459324148
1106	92-93	0.1980260002232228
1106	94-95	0.4111911560607737
1106	96-97	0.5698170852990359
1106	98-99	0.538578278906634
1106	100-101	0.2951491251918874
1107	1	0.2575558209030291
1107	2	0.16052219105139187
1107	3	0.163252380939376
1107	4	0.10425431878915958
1107	5	0.16613839620358561
1107	6	0.10530330046594116
1107	7	0.14574041542848448
1107	8	0.13005955700655392
1107	9	0.1509369941368277
1107	10-11	0.14155420478812886
1107	12-13	0.18520268419050723
1107	14-15	0.25716989686657854
1107	16-17	0.2589707516840676
1107	18-19	0.2687327138460134
1107	20-21	0.19764161781085932
1107	22-23	0.3047575769026949
1107	24-25	0.2676749937148415
1107	26-27	0.41321698560926023
1107	28-29	0.2993438946111624
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2316	90-91	-0.06638239978711624
2316	92-93	-0.007755935034538197
2316	94-95	0.024531251041459257
2316	96-97	-0.11199276436467187
2316	98-99	-0.14163550435618788
2316	100-101	0.047802842980861016
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	3.0
5	4.0
6	22.0
7	38.0
8	111.0
9	242.0
10	460.0
11	755.0
12	1158.0
13	1496.0
14	1832.0
15	2128.0
16	2374.0
17	2775.0
18	2659.0
19	2001.0
20	1713.0
21	1920.0
22	2825.0
23	3281.0
24	4624.0
25	5860.0
26	5491.0
27	4349.0
28	5417.0
29	6954.0
30	9312.0
31	12296.0
32	16214.0
33	21802.0
34	31428.0
35	49245.0
36	84626.0
37	184034.0
38	361007.0
39	162386.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99376517158369	22.60503016690001	15.611547023096062	22.78965763842024
2	14.20727937014979	22.25412337184969	41.73633783753844	21.802259420462075
3	16.75906651479858	27.17270563875738	33.36898406492864	22.6992437815154
4	12.169227964395326	19.47368580093897	40.043473399738716	28.31361283492699
5	12.124697129299355	38.329885860797305	37.30480826748896	12.240608742414386
6	27.84698458607167	39.4385487181243	19.46650446425874	13.24796223154528
7	23.46862732855454	34.01517425009919	25.15483414870926	17.361364272637005
8	22.147577336510242	36.55405146838161	23.655536063516745	17.642835131591404
9	23.216864485124844	17.211615733365022	24.015456224483835	35.5560635570263
10-11	21.837024683861078	27.525309744138198	31.77859550431673	18.85907006768399
12-13	22.336522668245724	25.524800175629935	31.226428680046364	20.912248476077973
14-15	20.132940985723017	28.277175056923635	27.906076251992708	23.68380770536064
16-17	19.97478309077332	30.661606500403582	29.246377964610726	20.11723244421237
18-19	19.808629738372694	29.79134367543088	30.67045050007729	19.729576086119135
20-21	20.253766875663395	28.99049142092935	30.546838966443033	20.208902736964223
22-23	20.183071869228336	29.12760146546116	30.2701516015138	20.419175063796704
24-25	20.36580134098961	29.365970525620394	30.03485404804441	20.23337408534559
26-27	20.25555025732771	29.199507147320553	30.33173899754732	20.21320359780442
28-29	20.15074334354537	29.016381555492444	30.33820570302917	20.49466939793302
30-31	20.8833565878631	28.780363274190794	30.135957176082695	20.20032296186341
32-33	20.24387799956999	29.2835876057972	29.938625161317283	20.533909233315526
34-35	20.505067850835427	29.307169622808072	30.061543404441537	20.12621912191496
36-37	20.3210524566621	29.08838585512104	29.992809327329695	20.597752360887174
38-39	20.19742235130383	28.582736319710612	30.353334044982972	20.866507284002587
40-41	20.274965307080176	28.47457900217322	30.50333434709838	20.747121343648224
42-43	20.715650988219515	28.498043299180864	30.148399090431198	20.637906622168423
44-45	20.596063637590408	28.84315980495429	29.860634160391058	20.700142397064244
46-47	20.71590275106294	28.506603235857476	29.727300558510695	21.05019345456889
48-49	20.671592490619318	28.4603292252351	29.781278512142016	21.08679977200357
50-51	20.43508647550146	28.59074237813168	29.833393420731962	21.140777725634898
52-53	20.45980958672626	28.92115593384881	29.888126662893583	20.730907816531353
54-55	20.55986014070522	28.705244119323503	29.623876382429774	21.111019357541505
56-57	20.647523962787435	28.91471080505701	29.332687477718988	21.105077754436564
58-59	20.354564254448796	28.998679246138064	29.95719508737973	20.68956141203341
60-61	20.796180781447323	29.272199384074977	29.38096312305006	20.550656711427646
62-63	20.15162731205213	29.730434738819312	29.348370170058264	20.769567779070293
64-65	20.278043813880135	29.69557077584184	29.140319021765947	20.886066388512077
66-67	20.280861902011544	29.526251997218374	29.289380513594175	20.903505587175907
68-69	20.24626344841338	29.544362769534754	29.239315542100847	20.970058239951015
70-71	20.247815701171	29.339745578028513	29.388084141325848	21.02435457947464
72-73	20.445042906470892	29.084952344887355	29.298565688621036	21.171439060020717
74-75	20.222391977694333	29.148637141635653	29.407768813860773	21.22120206680924
76-77	20.454127749739296	29.242568207532777	29.176705886973615	21.126598155754316
78-79	20.365923476917548	29.179567459839905	29.217130608477692	21.237378454764855
80-81	20.397981890595265	28.9635158088426	29.3162669911004	21.32223530946174
82-83	20.367857119476515	28.875786785082468	29.21867627233956	21.537679823101456
84-85	20.446598695632908	28.88450026788268	29.00947861571122	21.659422420773193
86-87	20.43853986547523	28.80610527736292	29.209337174979883	21.546017682181965
88-89	20.5423091467274	28.752727230614067	28.903584058119154	21.80137956453938
90-91	20.561874243452653	28.684146393044095	28.9355064159243	21.818472947578947
92-93	20.501149045617414	28.460077462391663	29.209374037007123	21.829399454983797
94-95	20.518873149794864	28.40247412381495	29.111186528069545	21.96746619832064
96-97	20.618178797026175	28.285468996649033	28.892016344452028	22.204335861872764
98-99	20.567937264664447	28.41045844045853	28.798727488602683	22.22287680627434
100-101	20.499079294473198	28.5905627305855	28.761209218435056	22.149148756506243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	799.0
1	488.0
2	196.5
3	188.0
4	128.0
5	113.5
6	177.0
7	305.5
8	451.0
9	490.0
10	358.0
11	222.0
12	200.0
13	216.5
14	221.5
15	238.0
16	268.5
17	297.5
18	328.5
19	380.5
20	425.5
21	471.5
22	575.5
23	706.5
24	856.0
25	1136.5
26	1828.0
27	3301.0
28	5938.5
29	10620.5
30	17601.0
31	25460.5
32	33177.5
33	40615.5
34	46595.0
35	50109.0
36	51875.5
37	52620.5
38	51565.5
39	47942.0
40	43924.0
41	40476.5
42	36849.5
43	34047.5
44	32593.5
45	31979.5
46	30970.5
47	29347.5
48	28332.0
49	27522.0
50	25825.0
51	23966.5
52	22041.5
53	20138.0
54	18126.0
55	16022.0
56	14035.5
57	12066.5
58	10629.5
59	9321.0
60	7768.5
61	6328.0
62	5137.5
63	3861.5
64	2882.5
65	2257.5
66	1711.0
67	1320.0
68	1045.5
69	798.5
70	602.5
71	468.0
72	364.5
73	308.0
74	237.5
75	179.0
76	132.5
77	77.5
78	55.5
79	39.5
80	36.0
81	29.0
82	18.0
83	14.0
84	7.5
85	5.0
86	5.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01893256582591304
2	0.0
3	0.0031218592585282147
4	0.020845963435978724
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.007051373718779E-4
12-13	1.007051373718779E-4
14-15	1.007051373718779E-4
16-17	0.0013595193545203514
18-19	1.5105770605781683E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.524679808015726E-4
28-29	0.00523666714333765
30-31	1.5105770605781683E-4
32-33	1.5105770605781683E-4
34-35	0.012235674190683162
36-37	0.004531731181734505
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	4.5317311817345054E-4
60-61	0.002014102747437558
62-63	0.010372629149303422
64-65	0.0025176284342969474
66-67	0.005488429986767345
68-69	0.0037260900827594816
70-71	2.014102747437558E-4
72-73	0.008308173833179926
74-75	0.00669689163522988
76-77	0.001762339904007863
78-79	3.524679808015726E-4
80-81	0.008912404657411192
82-83	0.011329327954336263
84-85	0.002618333571668825
86-87	0.0021148078848094356
88-89	3.524679808015726E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	5.035256868593895E-5
98-99	1.007051373718779E-4
100-101	9.566988050328399E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	992998.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.82067592580977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.147721989946376	25.918931570713326
2	19.42921861905153	17.02802985194333
3	8.43151492237404	11.08424048930946
4	4.326525600524705	7.583651049012505
5	2.463422944381991	5.397442925698367
6	1.5070315697367869	3.962348521644008
7	1.029524822603942	3.1580131526232895
8	0.7167932227567733	2.5128290816193073
9	0.5317311775784496	2.0970737651083002
>10	2.3284655088082027	16.80922505828783
>50	0.07260940091722491	2.0878872533132347
>100	0.01313571085634594	0.9097757789565927
>500	0.0011522552318106866	0.3248694083260653
>1k	9.218041854485493E-4	0.6041944886586411
>5k	2.3045104636213733E-4	0.5214876047857635
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	5164	0.5200413293883774	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	2453	0.24702970197321644	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT	1348	0.1357505251772914	RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1169	0.11772430558772526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.10201430415771232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0070513737187789E-4	0.0	0.0	0.0
6	0.0	1.0070513737187789E-4	0.0	0.0	0.0
7	0.0	1.0070513737187789E-4	0.0	0.0	0.0
8	0.0	1.0070513737187789E-4	0.0	0.0	0.0
9	0.0	1.0070513737187789E-4	0.0	0.0	0.0
10-11	0.0	1.0070513737187789E-4	0.0	0.0	0.0
12-13	0.0	2.0141027474375578E-4	0.0	0.0	0.0
14-15	0.0	2.5176284342969473E-4	0.0	0.0	0.0
16-17	0.0	3.0211541211563366E-4	0.0	0.0	0.0
18-19	0.0	3.0211541211563366E-4	0.0	0.0	0.0
20-21	0.0	3.0211541211563366E-4	0.0	1.5105770605781683E-4	0.0
22-23	0.0	5.538782555453284E-4	0.0	5.538782555453284E-4	0.0
24-25	0.0	6.042308242312673E-4	0.0	0.001007051373718779	0.0
26-27	0.0	6.042308242312673E-4	0.0	0.0015105770605781683	0.0
28-29	0.0	6.042308242312673E-4	0.0	0.0039275003575032375	0.0
30-31	0.0	6.042308242312673E-4	0.0	0.009164167500840887	0.0
32-33	0.0	6.042308242312673E-4	0.0	0.017522693902706752	0.0
34-35	0.0	8.056410989750231E-4	0.0	0.02522663691165541	0.0
36-37	0.0	0.001007051373718779	0.0	0.03494468266804163	0.0
38-39	0.0	0.0011581090797765958	0.0	0.04773423511427012	0.0
40-41	0.0	0.0012084616484625346	0.0	0.058660742519118875	0.0
42-43	0.0	0.0012084616484625346	0.0	0.07527709018547872	0.0
44-45	0.0	0.0016112821979500462	0.0	0.08645536043375716	0.0
46-47	0.0	0.0017119873353219242	0.0	0.10020161168501851	0.0
48-49	0.0	0.0017119873353219242	0.0	0.1282479924430865	0.0
50-51	0.0	0.0017119873353219242	0.0	0.15986940557785614	0.0
52-53	0.0	0.0017119873353219242	0.0	0.19415950485298056	0.0
54-55	0.0	0.0017119873353219242	0.0	0.22240729588579233	0.0
56-57	0.0	0.0017119873353219242	0.0	0.2891748019633473	0.0
58-59	0.0	0.0022155130221813138	0.0	0.36158179573372756	0.0
60-61	0.0	0.0024169232969250692	0.0	0.4202425382528464	0.0
62-63	0.0	0.0026686861403547642	0.0	0.46525773465807585	0.0
64-65	0.0	0.002819743846412581	0.0	0.5736668150389024	0.0
66-67	0.0	0.002920448983784459	0.0	0.7456711896700698	0.0
68-69	0.0	0.002920448983784459	0.0	0.9358528415968612	0.0
70-71	0.0	0.0030211541211563366	0.0	1.1386226356951372	0.0
72-73	0.0	0.0031218592585282147	0.0	1.3531245782972374	0.0
74-75	0.0	0.0031218592585282147	0.0	1.593356683497852	0.0
76-77	0.0	0.0031218592585282147	0.0	1.8497016106779671	0.0
78-79	0.0	0.0031218592585282147	0.0	2.138574297229199	0.0
80-81	0.0	0.0032225643959000925	0.0	2.444717914839708	0.0
82-83	0.0	0.0032225643959000925	0.0	2.759471821695512	0.0
84-85	0.0	0.0032225643959000925	0.0	3.089885377412643	0.0
86-87	0.0	0.00332326953327197	0.0	3.4634007319249385	0.0
88-89	0.0	0.00332326953327197	0.0	3.872364294792135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1115	0.0	37.920628	1
GTATCAA	1815	0.0	36.383003	1
TCAACGC	1860	0.0	33.456074	4
ACGCGTT	30	0.003950127	31.669144	28-29
CAACGCA	1965	0.0	31.665155	5
ATCAACG	1975	0.0	31.26748	3
AACGCAG	2005	0.0	31.033434	6
TATCAAC	2110	0.0	29.942347	2
GTGCGAG	80	4.4891617E-4	29.692064	1
ACGCAGA	2275	0.0	27.350346	7
AACGCGT	35	0.008389815	27.14498	28-29
CGCAGAG	2280	0.0	27.08204	8
CGGAGAT	145	7.8751873E-7	26.210924	1
TGGACCG	145	7.889048E-7	26.205645	5
TACCGTA	95	0.001226778	24.998808	7
GCAGAGT	2675	0.0	21.484955	9
GTACATG	1580	0.0	20.74686	1
GGACCGA	115	0.0037239557	20.65119	6
AGTACGT	115	0.0037239557	20.65119	5
GAGTACT	1775	0.0	19.80287	12-13
>>END_MODULE