##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031684_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98423 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.124300214380785 33.0 31.0 34.0 26.0 34.0 2 30.291527386891275 33.0 31.0 34.0 26.0 34.0 3 30.19446674049765 33.0 31.0 34.0 25.0 34.0 4 33.58476169188096 37.0 35.0 37.0 30.0 37.0 5 33.57735488656107 37.0 35.0 37.0 28.0 37.0 6 33.723855196447985 37.0 35.0 37.0 30.0 37.0 7 33.71445698667994 37.0 35.0 37.0 30.0 37.0 8 33.70893998354043 37.0 35.0 37.0 30.0 37.0 9 35.21068246243256 39.0 37.0 39.0 29.0 39.0 10-11 35.14930961259056 39.0 37.0 39.0 27.5 39.0 12-13 35.030338437153915 39.0 37.0 39.0 27.0 39.0 14-15 35.76602013756947 40.0 36.5 41.0 26.0 41.0 16-17 35.69686963413024 39.5 36.5 41.0 26.0 41.0 18-19 36.065807788829844 40.0 37.5 41.0 27.0 41.0 20-21 36.30785487132073 40.5 38.0 41.0 27.0 41.0 22-23 36.17733659815287 41.0 38.0 41.0 27.0 41.0 24-25 35.99667760584416 40.5 37.0 41.0 26.5 41.0 26-27 35.72610060656554 40.0 36.5 41.0 25.0 41.0 28-29 35.43940440750637 40.0 35.0 41.0 24.0 41.0 30-31 34.740660211535925 39.0 35.0 41.0 19.0 41.0 32-33 33.8378681812178 37.0 35.0 41.0 12.0 41.0 34-35 33.287478536520936 36.0 35.0 40.5 3.5 41.0 36-37 32.86387328165165 35.0 35.0 40.0 2.0 41.0 38-39 32.3715544130945 35.0 35.0 40.0 2.0 41.0 40-41 31.70054763622324 35.0 33.5 40.0 2.0 41.0 42-43 30.801199922782278 35.0 33.0 40.0 2.0 41.0 44-45 29.449356349633725 35.0 26.5 40.0 2.0 41.0 46-47 27.42101947715473 35.0 2.0 40.0 2.0 41.0 48-49 25.686709407353973 35.0 2.0 40.0 2.0 41.0 50-51 24.60612864879144 35.0 2.0 39.5 2.0 40.5 52-53 24.27787712221737 35.0 2.0 40.0 2.0 41.0 54-55 24.157209188909096 35.0 2.0 40.0 2.0 41.0 56-57 23.833026833158915 35.0 2.0 40.0 2.0 41.0 58-59 23.501869481726835 35.0 2.0 40.0 2.0 41.0 60-61 23.132423315688406 35.0 2.0 40.0 2.0 41.0 62-63 22.729605884803348 34.0 2.0 39.0 2.0 41.0 64-65 22.370863517673715 34.0 2.0 39.0 2.0 41.0 66-67 22.010282149497577 33.0 2.0 38.5 2.0 41.0 68-69 21.600753888826798 33.0 2.0 37.0 2.0 40.5 70-71 21.158712902471983 32.5 2.0 37.0 2.0 40.0 72-73 20.75341637625352 31.5 2.0 36.0 2.0 39.0 74-75 20.315139753919308 31.0 2.0 35.5 2.0 39.0 76-77 19.89720898570456 29.5 2.0 35.0 2.0 38.0 78-79 19.516810095201325 29.0 2.0 35.0 2.0 37.0 80-81 19.111249403086678 26.5 2.0 35.0 2.0 37.0 82-83 18.72698454629507 25.0 2.0 35.0 2.0 36.0 84-85 18.336887719333895 19.0 2.0 35.0 2.0 36.0 86-87 17.994889405931538 6.5 2.0 35.0 2.0 35.5 88-89 17.633566341200734 2.0 2.0 35.0 2.0 35.0 90-91 17.26393729108034 2.0 2.0 35.0 2.0 35.0 92-93 16.898697458927284 2.0 2.0 35.0 2.0 35.0 94-95 16.499781555124308 2.0 2.0 35.0 2.0 35.0 96-97 16.117066132916086 2.0 2.0 35.0 2.0 35.0 98-99 15.719968909706065 2.0 2.0 35.0 2.0 35.0 100-101 15.322952968310252 2.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9672860768686 1101 2 -1.8634063658395412 1101 3 -1.8702093581236099 1101 4 -2.1584531169317422 1101 5 -1.5912960058459902 1101 6 -1.912289808495423 1101 7 -1.4002560396659902 1101 8 -2.578216284726526 1101 9 -1.9502897598304259 1101 10-11 -2.6054641919537787 1101 12-13 -2.105825074759082 1101 14-15 -2.5323056723703488 1101 16-17 -2.021276291534278 1101 18-19 -2.848941391203475 1101 20-21 -2.5541716697360854 1101 22-23 -2.383050237050945 1101 24-25 -2.2227435457514773 1101 26-27 -2.661242857391862 1101 28-29 -3.1930511480178154 1101 30-31 -2.9346678523434946 1101 32-33 -2.6954048789009803 1101 34-35 -2.8066213629863626 1101 36-37 -1.8003511766273874 1101 38-39 -1.4734398116280687 1101 40-41 -1.3854442109673464 1101 42-43 -2.6836456394392947 1101 44-45 -2.9341707871822216 1101 46-47 -2.649726379775686 1101 48-49 -3.1487367124726475 1101 50-51 -2.4817415932044042 1101 52-53 -1.7104465948613168 1101 54-55 -1.2706511925382635 1101 56-57 -1.7594779861067522 1101 58-59 -2.4800171752836064 1101 60-61 -1.8557547946132402 1101 62-63 -1.6088682993334622 1101 64-65 -1.2097208445047585 1101 66-67 -1.9331915585752668 1101 68-69 -1.7235461540905028 1101 70-71 -1.7714755667475295 1101 72-73 -1.971086491961433 1101 74-75 -2.0448331180578627 1101 76-77 -2.1662412683367442 1101 78-79 -2.2524486468132174 1101 80-81 -2.1807967971851774 1101 82-83 -1.907544314847975 1101 84-85 -1.11305011625684 1101 86-87 -1.082130545200581 1101 88-89 -1.2554363381379439 1101 90-91 -1.4170343721392413 1101 92-93 -1.5550679778642902 1101 94-95 -1.5463549674886359 1101 96-97 -2.1885245990136912 1101 98-99 -2.164391461999301 1101 100-101 -2.021153680600161 1102 1 -0.5074345249117833 1102 2 -0.014216082438732514 1102 3 0.18647080381970937 1102 4 0.2551771124879636 1102 5 0.5254381372039418 1102 6 -0.04656781119447828 1102 7 -0.047017173269225054 1102 8 -0.5670826814876548 1102 9 -0.8268079784539069 1102 10-11 0.2219649578437881 1102 12-13 -0.36577784129080015 1102 14-15 0.00818015758917312 1102 16-17 -0.3193532146111977 1102 18-19 -0.0459724303397806 1102 20-21 -0.4313646521922294 1102 22-23 -0.13186940034379546 1102 24-25 0.07347777678563716 1102 26-27 -0.6740633702123731 1102 28-29 -0.4224708511217301 1102 30-31 -0.8381766242733164 1102 32-33 -0.6198313296432261 1102 34-35 -1.738807597804179 1102 36-37 -1.3313903129296776 1102 38-39 -1.3410174094688259 1102 40-41 -0.8711054795233544 1102 42-43 -1.5289560308023233 1102 44-45 -1.947328681919064 1102 46-47 -1.6900435187770313 1102 48-49 -1.16189460720949 1102 50-51 -0.1521194609506935 1102 52-53 -0.48963013062380156 1102 54-55 -0.5761505177744333 1102 56-57 -0.6648423585763865 1102 58-59 -0.8133505086169421 1102 60-61 -0.8360179525079765 1102 62-63 -1.1486793654603211 1102 64-65 -0.5513200347881551 1102 66-67 -0.6007016800327598 1102 68-69 -1.9111304995695875 1102 70-71 -2.1012663899594024 1102 72-73 -1.4149124028926074 1102 74-75 -1.9834296092859347 1102 76-77 -1.4690415382422763 1102 78-79 -1.0873001987700341 1102 80-81 -1.526950643339024 1102 82-83 -1.5769100368452733 1102 84-85 -1.6516803456765423 1102 86-87 -1.554971300936078 1102 88-89 -0.8944376876656079 1102 90-91 -1.1827226312485521 1102 92-93 -1.5709249279317667 1102 94-95 -1.6226032805790531 1102 96-97 -1.898544841928672 1102 98-99 -1.8513010436457247 1102 100-101 -1.4963561097499607 1103 1 -0.17057555055280815 1103 2 0.25501468679203754 1103 3 0.16531695766586196 1103 4 0.098125830436679 1103 5 0.2863355731013826 1103 6 -0.36163191375857906 1103 7 -0.2614402501923081 1103 8 0.20599424158926638 1103 9 0.3529997138537837 1103 10-11 -0.023227349848518486 1103 12-13 -0.018982969495930035 1103 14-15 0.14664169605070754 1103 16-17 0.20997370846572494 1103 18-19 0.17934808248072898 1103 20-21 -0.2271979855255708 1103 22-23 -0.10574760547199702 1103 24-25 -0.45761196680410876 1103 26-27 0.11375714260813652 1103 28-29 -0.09041956907044835 1103 30-31 0.059740042393350734 1103 32-33 -0.029286457848350267 1103 34-35 0.13186547911890045 1103 36-37 1.11412250758314 1103 38-39 1.272283872582456 1103 40-41 0.8175163153484384 1103 42-43 1.286749097402808 1103 44-45 0.9464213180809331 1103 46-47 1.4591551991716827 1103 48-49 2.0006053927905114 1103 50-51 2.6610215646903335 1103 52-53 2.295145510401838 1103 54-55 2.0836251232512097 1103 56-57 1.4688114875774616 1103 58-59 1.1449828247163936 1103 60-61 0.9514820474920249 1103 62-63 1.7572501217191672 1103 64-65 2.2054107344426086 1103 66-67 2.2589137045826284 1103 68-69 2.5794143722252834 1103 70-71 2.5291823279893144 1103 72-73 1.6259529817227794 1103 74-75 1.7124037240473982 1103 76-77 1.0120482053474653 1103 78-79 0.907090826870995 1103 80-81 0.8129532028148212 1103 82-83 0.6246925272572881 1103 84-85 -0.21337906362526127 1103 86-87 0.027079981115207374 1103 88-89 -0.05148896971689254 1103 90-91 -0.2288764774024017 1103 92-93 -0.7922390304958675 1103 94-95 -0.6075391780149513 1103 96-97 -0.3230640726979015 1103 98-99 -0.29136514620982545 1103 100-101 -0.2727984174422673 1104 1 0.26322074574348875 1104 2 0.7303233287673478 1104 3 0.6427860934683309 1104 4 1.1521381761156917 1104 5 1.300841745940886 1104 6 0.8693866047599386 1104 7 0.5365535769681884 1104 8 0.2942658465275372 1104 9 0.025993541014280197 1104 10-11 0.4019269711391402 1104 12-13 0.5430540675411137 1104 14-15 1.2349133009889783 1104 16-17 0.7919953134039943 1104 18-19 0.417465366431351 1104 20-21 0.1735736194127071 1104 22-23 -0.39563958078064587 1104 24-25 -0.2349267816189169 1104 26-27 -0.21062557344124144 1104 28-29 -0.10029611228031854 1104 30-31 -0.0542260069893743 1104 32-33 -0.06285127266315982 1104 34-35 0.10972041739050553 1104 36-37 -0.11890218377488537 1104 38-39 -0.12378094223235792 1104 40-41 -0.7120361537873627 1104 42-43 -0.632232384078673 1104 44-45 0.16995526869822086 1104 46-47 -0.07703307243325241 1104 48-49 0.7763769977287822 1104 50-51 1.2994474906162559 1104 52-53 1.6328769918833217 1104 54-55 1.179072654115405 1104 56-57 0.7245213641206689 1104 58-59 0.6496124543460233 1104 60-61 1.0929943931710362 1104 62-63 0.8508458007315127 1104 64-65 1.1747008578994027 1104 66-67 0.8116914823604056 1104 68-69 0.7384421500030633 1104 70-71 0.9777934391004273 1104 72-73 0.5283449570314218 1104 74-75 1.254224711701724 1104 76-77 0.24059758806351894 1104 78-79 -0.46289374103023917 1104 80-81 -0.34129062434567103 1104 82-83 -0.37391858385382193 1104 84-85 -0.36962906362526127 1104 86-87 -0.529092858390964 1104 88-89 -0.3925383524329433 1104 90-91 -0.4146789465382028 1104 92-93 -0.47287174654524833 1104 94-95 -0.21757004221248266 1104 96-97 -0.21573382578432287 1104 98-99 0.1788509031728882 1104 100-101 0.5910133109527962 1105 1 -1.2205755505528089 1105 2 -0.8074853132079625 1105 3 -0.7221830423341373 1105 4 -0.9768741695633238 1105 5 -0.8261644268986217 1105 6 -0.8991319137585805 1105 7 -0.5989402501923067 1105 8 -1.0440057584107336 1105 9 -1.1345002861462135 1105 10-11 -1.1482273498485185 1105 12-13 -1.581482969495923 1105 14-15 -1.5283583039492967 1105 16-17 -1.1587762915342807 1105 18-19 -1.633151917519271 1105 20-21 -1.9584479855255665 1105 22-23 -1.8494976054720027 1105 24-25 -1.4513619668041073 1105 26-27 -1.4674928573918677 1105 28-29 -1.6904195690704427 1105 30-31 -1.309009957606655 1105 32-33 -1.3605364578483474 1105 34-35 -1.430634520881103 1105 36-37 -2.2358774924168614 1105 38-39 -2.0027161274175427 1105 40-41 -1.5699836846515574 1105 42-43 -1.1882509025971935 1105 44-45 -2.2348286819190655 1105 46-47 -1.3095948008283145 1105 48-49 -1.5618946072094886 1105 50-51 -1.5264784353096665 1105 52-53 -1.42985448959816 1105 54-55 -0.49762487674879097 1105 56-57 -0.22493851242253982 1105 58-59 -0.055017175283605724 1105 60-61 -0.0860179525079765 1105 62-63 -0.16774987828083354 1105 64-65 0.1429107344426086 1105 66-67 0.008913704582628412 1105 68-69 0.14191437222528336 1105 70-71 -0.21456767201068772 1105 72-73 -0.5552970182772192 1105 74-75 -0.4063462759526004 1105 76-77 0.1682982053474653 1105 78-79 0.2883408268709964 1105 80-81 0.08795320281481978 1105 82-83 -0.07530747274271121 1105 84-85 -0.019629063625259846 1105 86-87 -0.07917001888479192 1105 88-89 -0.44523896971689325 1105 90-91 -1.0663764774024003 1105 92-93 -0.9984890304958682 1105 94-95 -1.1825391780149506 1105 96-97 -1.3855640726979033 1105 98-99 -2.0226151462098265 1105 100-101 -1.9102984174422666 1106 1 0.49753669434515047 1106 2 0.6019534623022409 1106 3 0.9321536923597407 1106 4 0.14659521819178423 1106 5 0.22664169555035585 1106 6 0.3202558413434602 1106 7 0.2944270967464675 1106 8 0.4580350579157937 1106 9 0.4471323669150138 1106 10-11 0.16937469096780688 1106 12-13 -0.11643194908776167 1106 14-15 0.09256006339764866 1106 16-17 0.4251522798942915 1106 18-19 -0.06886620323356141 1106 20-21 -0.16775921001536176 1106 22-23 0.2548391292218781 1106 24-25 0.95233701278773 1106 26-27 0.8846755099550805 1106 28-29 0.5789681860315952 1106 30-31 0.4662451444341613 1106 32-33 0.7264533380700158 1106 34-35 1.5728093566699215 1106 36-37 1.3207551606443602 1106 38-39 1.5073604031947028 1106 40-41 1.56662345820558 1106 42-43 2.3594531790354587 1106 44-45 1.5832835629788953 1106 46-47 2.0585174440696434 1106 48-49 1.1493298825864287 1106 50-51 1.9602562585678847 1106 52-53 2.6105791838712307 1106 54-55 2.3505894089654937 1106 56-57 2.206821691659094 1106 58-59 1.1424318043082309 1106 60-61 1.2150024556552879 1106 62-63 0.07829604008651359 1106 64-65 -0.5831096737206529 1106 66-67 -0.14975976480512898 1106 68-69 -0.37607032165226784 1106 70-71 -0.7404605291535447 1106 72-73 -0.902745997869058 1106 74-75 -1.1347901535036193 1106 76-77 -0.7815742436321251 1106 78-79 -0.5820673363943136 1106 80-81 -0.3848784298382384 1106 82-83 0.17086599664504476 1106 84-85 0.4211872629053488 1106 86-87 0.07401875662541002 1106 88-89 0.10412327518106679 1106 90-91 0.12928678790372317 1106 92-93 0.13263341848372434 1106 94-95 0.7197567403523948 1106 96-97 1.0339512334245455 1106 98-99 1.18388995583099 1106 100-101 1.134727092761814 1107 1 -1.3540632048737962 1107 2 -1.553627288516605 1107 3 -1.5053620546798179 1107 4 -1.2429235522793718 1107 5 -1.6250841799850413 1107 6 -1.525675123635125 1107 7 -1.7473970403157608 1107 8 -1.5205489682872795 1107 9 -1.430796582442511 1107 10-11 -1.3881964856509867 1107 12-13 -0.654476796656418 1107 14-15 -1.5613829953073193 1107 16-17 -1.9672639458552652 1107 18-19 -1.5578432755439593 1107 20-21 -1.480747368241616 1107 22-23 -1.506750691891753 1107 24-25 -1.1855440655695375 1107 26-27 -1.5686502647992668 1107 28-29 -1.303999815984028 1107 30-31 -0.8381766242733235 1107 32-33 -1.4517401615520527 1107 34-35 -1.2236129159428302 1107 36-37 -0.6744577393304425 1107 38-39 -0.6052624237138389 1107 40-41 -0.47129541304662226 1107 42-43 -0.632232384078673 1107 44-45 0.14526391067353117 1107 46-47 0.7748187794185952 1107 48-49 0.1714387261238457 1107 50-51 0.5648795893816896 1107 52-53 0.6328769918833217 1107 54-55 0.07413438251046855 1107 56-57 -0.3557255494595779 1107 58-59 -0.41828878022187865 1107 60-61 0.6176857511957259 1107 62-63 -0.4269319770462623 1107 64-65 -0.2635707470388695 1107 66-67 -0.10806160405934762 1107 68-69 -0.3726689611080509 1107 70-71 -0.6703547090477251 1107 72-73 -0.35437109235129327 1107 74-75 -0.5112073870637097 1107 76-77 -0.9260690786031525 1107 78-79 -1.0307949755981411 1107 80-81 -0.686969636691348 1107 82-83 -0.7504617937303664 1107 84-85 -0.6535796809092105 1107 86-87 -0.7636607596255338 1107 88-89 -0.8801926734205949 1107 90-91 -0.8591233909826457 1107 92-93 -1.6025013761748816 1107 94-95 -2.0323848570272958 1107 96-97 -2.4749930850435806 1107 98-99 -1.9569515659629122 1107 100-101 -1.7052829853435014 1108 1 0.07330742817059388 1108 2 -0.0854108451228548 1108 3 0.06372121298501199 1108 4 0.03695561767072064 1108 5 0.06771855182478248 1108 6 -0.2767914882266638 1108 7 0.06754911150981968 1108 8 -0.27166533287881833 1108 9 -0.6132236904015329 1108 10-11 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88-89 -0.9333382847853855 2316 90-91 -1.0049038746626735 2316 92-93 -1.248831496249295 2316 94-95 -1.2837378081519368 2316 96-97 -1.1361633877663948 2316 98-99 -1.1113993927851684 2316 100-101 -1.1088429379902127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5429.0 3 492.0 4 372.0 5 314.0 6 293.0 7 343.0 8 356.0 9 414.0 10 440.0 11 675.0 12 1098.0 13 1648.0 14 1865.0 15 2935.0 16 4397.0 17 6981.0 18 6384.0 19 2525.0 20 1705.0 21 1341.0 22 1138.0 23 1125.0 24 1046.0 25 1088.0 26 1188.0 27 1168.0 28 1314.0 29 1352.0 30 1634.0 31 1779.0 32 1966.0 33 2318.0 34 3070.0 35 5084.0 36 5838.0 37 10107.0 38 13618.0 39 3574.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.87189401493722 18.30419829463359 15.140723254441482 11.683184435987707 2 20.28736275477496 22.68476590721765 40.07102662989834 16.956844708109042 3 21.235030168598204 33.839704520711024 32.14354758295093 12.781717727739847 4 13.165097939455608 23.82192826252862 33.405240396845585 29.607733401170183 5 9.567898094356323 39.816048917964736 31.265579374688414 19.350473612990527 6 20.41910380957225 39.250292457148674 17.834291236457965 22.49631249682112 7 20.043338046939375 45.144816220230524 19.43090836953315 15.380937363296947 8 27.663967776059888 30.800919521523312 17.552282529091055 23.982830173325738 9 31.626861420782816 15.709170803157296 18.862397265847505 33.801570510212386 10-11 24.763793541825578 30.43579964403763 20.492753623188406 24.307653190948386 12-13 31.227079677526003 31.704178428829376 23.917499554945195 13.151242338699424 14-15 27.879577156694168 28.56532400012209 30.294443822682553 13.260655020501186 16-17 15.795230802887772 33.15238129156003 31.20481498628869 19.847572919263506 18-19 14.756308506308507 24.815832315832314 44.03184778184778 16.396011396011396 20-21 20.315305919998373 20.9064409297404 45.628805876757 13.149447273504231 22-23 19.456949128402336 22.26325454110777 45.547908623399614 12.731887707090284 24-25 16.579272603359797 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17.00709688906977 50-51 18.209749373051118 31.056987491543186 33.210741300289435 17.522521835116258 52-53 17.895523830352104 31.837208001261324 32.468886526734444 17.798381641652128 54-55 17.81987388120423 32.2909886086249 31.927379983726606 17.961757526444263 56-57 17.52833221428717 32.558648192231786 31.61864941300942 18.294370180471628 58-59 17.440062658298665 33.12192938735238 31.425272858581437 18.012735095767514 60-61 16.913507410308316 33.084966788391704 31.76972606780661 18.23179973349337 62-63 16.99843355846692 33.005126535926436 31.68077142159655 18.31566848401009 64-65 16.934511545564153 33.27686461242617 31.61536152699561 18.173262315014068 66-67 16.908200273664615 33.75536011312827 30.951060831879385 18.38537878132773 68-69 16.92940823375606 34.06890798195146 30.76594380942207 18.23573997487041 70-71 16.809253023814126 33.924193564794564 30.698452743049554 18.56810066834176 72-73 16.413621220213116 33.9701431804888 30.62333104448005 18.992904554818036 74-75 16.233987479849272 34.38922310989966 30.349923465366167 19.026865944884893 76-77 16.169678101981848 34.34714931021853 30.30409392422578 19.179078663573843 78-79 16.264294143979164 34.526716314654294 29.78370569324055 19.425283848125993 80-81 15.933889846933324 34.846704411921806 29.653425305599217 19.56598043554565 82-83 15.88398984580478 34.92310588139534 29.629290478152708 19.56361379464717 84-85 15.936050297061936 34.74709042076992 29.428460975014243 19.888398307153903 86-87 15.996947729874094 34.830471830090296 28.9451863156556 20.22739412438001 88-89 16.268317370538597 34.980646277015104 28.498547834977085 20.252488517469214 90-91 16.293549420867098 34.840045374312645 28.616337804636117 20.250067400184143 92-93 16.32639563693344 34.71069755137135 28.787285242598916 20.175621569096304 94-95 16.294517969950054 34.71358961577978 28.58458032816903 20.407312086101136 96-97 16.442912064441327 34.66365615020036 28.21901507292366 20.674416712434653 98-99 16.843823744812433 34.527321181544465 27.877064854748152 20.751790218894946 100-101 17.394047316204528 34.46451284660392 27.455609259730345 20.685830577461207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 891.0 1 500.5 2 123.0 3 130.0 4 98.0 5 90.0 6 163.5 7 299.5 8 427.5 9 490.0 10 462.5 11 424.0 12 463.5 13 551.0 14 640.0 15 707.0 16 794.0 17 913.0 18 1015.5 19 1137.0 20 1262.5 21 1396.0 22 1566.0 23 1707.0 24 1878.5 25 2078.5 26 2240.5 27 2449.0 28 2669.0 29 2788.0 30 2831.0 31 2893.0 32 3050.5 33 3162.0 34 3225.0 35 3243.0 36 3177.0 37 3164.0 38 3112.0 39 2941.5 40 2800.0 41 2670.5 42 2492.5 43 2374.0 44 2287.5 45 2145.5 46 2073.5 47 2007.5 48 2023.5 49 1980.0 50 1919.5 51 1824.5 52 1511.0 53 1286.0 54 1149.0 55 1006.5 56 851.5 57 775.0 58 688.5 59 555.0 60 479.5 61 425.5 62 340.0 63 289.0 64 258.0 65 225.5 66 200.0 67 162.5 68 131.0 69 114.5 70 119.5 71 107.0 72 77.5 73 71.5 74 58.0 75 41.0 76 43.5 77 40.5 78 26.0 79 19.0 80 16.5 81 13.0 82 11.0 83 8.5 84 5.0 85 2.0 86 3.0 87 2.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14732328825579388 2 0.15240340164392469 3 0.1442752202229154 4 0.15037135628867238 5 0.13817908415715838 6 0.11989067595988742 7 0.12801885738089674 8 0.11277851721650427 9 0.11277851721650427 10-11 0.09957022240736414 12-13 0.12243073265395282 14-15 0.13817908415715838 16-17 0.14884732227223313 18-19 0.14529124290054155 20-21 0.13970311817359762 22-23 0.12852686871970984 24-25 0.1153185739105697 26-27 0.11887465328226125 28-29 0.1239547666703921 30-31 0.11328652855531736 32-33 0.11328652855531736 34-35 0.12903488005852293 36-37 0.13360698210784064 38-39 0.117350619265822 40-41 0.11938266462107434 42-43 0.1153185739105697 44-45 0.1346230047854668 46-47 0.13106692541377524 48-49 0.14275118620647614 50-51 0.13157493675258833 52-53 0.1153185739105697 54-55 0.10465033579549496 56-57 0.1270028347032706 58-59 0.11379453989413042 60-61 0.11582658524938276 62-63 0.11277851721650427 64-65 0.1407191408512238 66-67 0.129542891397336 68-69 0.1346230047854668 70-71 0.12192272131513976 72-73 0.12243073265395282 74-75 0.10312630177905571 76-77 0.13309897076902755 78-79 0.13309897076902755 80-81 0.1346230047854668 82-83 0.1407191408512238 84-85 0.12903488005852293 86-87 0.1376710728183453 88-89 0.123446755331579 90-91 0.13157493675258833 92-93 0.14579925423935464 94-95 0.12192272131513976 96-97 0.10261829044024262 98-99 0.11277851721650427 100-101 0.15037135628867238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 98423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.54800199140445 #Duplication Level Percentage of deduplicated Percentage of total 1 87.38197933804675 59.89860093677291 2 8.795411089866157 12.05815713806732 3 2.15068107371011 4.422746715706695 4 0.6314198052381165 1.7313026426749847 5 0.2653148946892555 0.9093402964754173 6 0.15711384825173788 0.6461904229702408 7 0.0904145730505284 0.4338416833463723 8 0.06373486297004462 0.34951180110340063 9 0.05780603850771488 0.3566239598467838 >10 0.3260853454281352 4.265263200674639 >50 0.04001956512072569 2.017821037765563 >100 0.032608534542813525 4.228686384280097 >500 0.002964412231164866 1.708950143767209 >1k 0.004446618346747299 6.972963636548368 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2868 2.9139530394318403 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2778 2.8225109984454853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 1.2364995986710423 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.9723337024882395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 725 0.7366164412789694 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.3932007762413257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 324 0.32919134755087737 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 281 0.2855023724129523 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.259085782794672 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 243 0.246893510663158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 243 0.246893510663158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 233 0.23673328388689635 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.22047692104487773 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 182 0.18491612732796198 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 180 0.18288408197270964 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 168 0.17069180984119564 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 159 0.1615476057425602 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 156 0.15849953770968167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 156 0.15849953770968167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 152 0.15443544699917702 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.13716306147953222 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 123 0.12497078934801825 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 122 0.1239547666703921 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 120 0.12192272131513976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 114 0.11582658524938276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 110 0.11176249453887811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 102 0.10363431311786878 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0010160226776261647 0.0 5 0.0 0.0 0.0 0.0010160226776261647 0.0 6 0.0 0.0 0.0 0.0010160226776261647 0.0 7 0.0 0.0 0.0 0.0010160226776261647 0.0 8 0.0 0.0 0.0 0.0010160226776261647 0.0 9 0.0 0.0 0.0 0.0010160226776261647 0.0 10-11 0.0 0.0 0.0 0.0010160226776261647 0.0 12-13 0.0 0.0 0.0 0.0010160226776261647 0.0 14-15 0.0 0.0 0.0 0.0015240340164392472 0.0 16-17 0.0 0.0 0.0 0.0020320453552523294 0.0 18-19 0.0 0.0 0.0 0.0020320453552523294 0.0 20-21 0.0 0.0 0.0 0.004572102049317741 0.0 22-23 0.0 0.0 0.0 0.009144204098635481 0.0 24-25 0.0 0.0 0.0 0.01320829480914014 0.0 26-27 0.0 0.0 0.0 0.021336476230149455 0.0 28-29 0.0 0.0 0.0 0.05029312254249515 0.0 30-31 0.0 0.0 0.0 0.08534590492059782 0.0 32-33 0.0 0.0 0.0 0.14579925423935464 0.0 34-35 0.0 0.0 0.0 0.2540056694065411 0.0 36-37 0.0 0.0 0.0 0.3906607195472603 0.0 38-39 0.0 0.0 0.0 0.4973431006980076 0.0 40-41 0.0 0.0 0.0 0.5745608241975961 0.0 42-43 0.0 0.0 0.0 0.6675268992003902 0.0 44-45 0.0 0.0 0.0 0.7874175751602775 0.0 46-47 0.0 0.0 0.0 0.9240726253009968 0.0 48-49 0.0 0.0 0.0 1.0795240949778 0.0 50-51 0.0 0.0 0.0 1.1938266462107434 0.0 52-53 0.0 0.0 0.0 1.3223535149304533 0.0 54-55 0.0 0.0 0.0 1.481361063978948 0.0 56-57 0.0 0.0 0.0 1.6840575881653679 0.0 58-59 0.0 0.0 0.0 1.856781443361816 0.0 60-61 0.0 0.0 0.0 2.038141491318086 0.0 62-63 0.0 0.0 0.0 2.241854038182132 0.0 64-65 0.0 0.0 0.0 2.4740152200197105 0.0 66-67 0.0 0.0 0.0 2.75240543368928 0.0 68-69 0.0 0.0 0.0 3.0755006451744 0.0 70-71 0.0 0.0 0.0 3.382847505156315 0.0 72-73 0.0 0.0 0.0 3.6526015260660616 0.0 74-75 0.0 0.0 0.0 3.9574083293539113 0.0 76-77 0.0 0.0 0.0 4.265263200674639 0.0 78-79 0.0 0.0 0.0 4.6213791491826095 0.0 80-81 0.0 0.0 0.0 4.95920668949331 0.0 82-83 0.0 0.0 0.0 5.333103034859738 0.0 84-85 0.0 0.0 0.0 5.628257622710139 0.0 86-87 0.0 0.0 0.0 5.984881582556923 0.0 88-89 0.0 0.0 0.0 6.431423549373623 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 59.73244 1 GTGGTAT 495 0.0 49.898994 1 TGGTATC 510 0.0 48.431374 2 GTATCAA 2620 0.0 39.5229 1 TATCAAC 3330 0.0 31.38138 2 ATCAACG 3465 0.0 30.15873 3 CTCGCCA 40 4.4802512E-4 29.687498 44-45 TCAACGC 3530 0.0 29.6034 4 CAACGCA 3625 0.0 28.696552 5 AACGCAG 3700 0.0 28.243242 6 TGGGGTG 155 4.4983608E-8 27.580645 6 TTCTCGC 35 0.008374955 27.142859 42-43 ATGGGTA 130 3.2459138E-7 25.576923 5 ACGCAGA 4120 0.0 25.248787 7 CGCAGAG 4170 0.0 25.173859 8 GAGTACT 2510 0.0 24.41235 12-13 GCAGAGT 4205 0.0 24.173603 9 GTACTTT 2630 0.0 23.298481 14-15 CAGAGTA 4140 0.0 22.373188 10-11 AGTACTT 2510 0.0 22.046812 12-13 >>END_MODULE