##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031684_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98423 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41700618757811 33.0 31.0 34.0 31.0 34.0 2 32.538044969163714 34.0 31.0 34.0 31.0 34.0 3 32.387765054916024 34.0 31.0 34.0 30.0 34.0 4 36.02469951129309 37.0 35.0 37.0 35.0 37.0 5 36.05994533797995 37.0 35.0 37.0 35.0 37.0 6 36.20380398890503 37.0 37.0 37.0 35.0 37.0 7 36.16595714416346 37.0 37.0 37.0 35.0 37.0 8 36.1910630645276 37.0 37.0 37.0 35.0 37.0 9 37.85525740934538 39.0 38.0 39.0 35.0 39.0 10-11 37.880937382522376 39.0 38.0 39.0 35.0 39.0 12-13 37.775530109832054 39.0 38.0 39.0 35.0 39.0 14-15 38.689620312325374 40.0 38.0 41.0 34.5 41.0 16-17 38.655166983327064 40.0 38.0 41.0 35.0 41.0 18-19 39.11866636863334 40.0 38.5 41.0 35.5 41.0 20-21 39.37350517663554 41.0 39.0 41.0 36.0 41.0 22-23 39.262149091167714 41.0 39.0 41.0 35.0 41.0 24-25 39.08806376558324 41.0 39.0 41.0 35.0 41.0 26-27 38.74458205907156 40.5 38.0 41.0 35.0 41.0 28-29 38.418636903975695 40.0 37.5 41.0 35.0 41.0 30-31 37.62789693465958 39.5 36.0 41.0 34.5 41.0 32-33 36.5140820743119 37.5 35.0 41.0 33.0 41.0 34-35 35.87631447933918 37.0 35.0 40.5 33.0 41.0 36-37 35.32964855775581 36.5 35.0 40.5 31.5 41.0 38-39 34.686175995448224 35.5 35.0 40.0 28.5 41.0 40-41 34.09478475559574 36.0 35.0 40.0 23.0 41.0 42-43 33.18340733365169 35.5 34.5 40.0 4.5 41.0 44-45 31.664453430600574 35.0 33.0 40.0 2.0 41.0 46-47 29.37783851335562 35.0 25.0 40.0 2.0 41.0 48-49 27.24085833595806 35.0 2.0 40.0 2.0 41.0 50-51 25.79333590725745 35.0 2.0 40.0 2.0 41.0 52-53 25.394841652865694 35.0 2.0 40.0 2.0 41.0 54-55 25.14183676579661 35.0 2.0 40.0 2.0 41.0 56-57 24.56546742123284 35.0 2.0 40.0 2.0 41.0 58-59 23.996479481422025 35.0 2.0 40.0 2.0 41.0 60-61 23.380668136512806 34.5 2.0 39.0 2.0 41.0 62-63 22.72498298162015 33.5 2.0 39.0 2.0 41.0 64-65 21.97578817959217 33.0 2.0 38.5 2.0 41.0 66-67 21.033686231876697 31.0 2.0 37.5 2.0 41.0 68-69 20.10961868668909 28.5 2.0 37.0 2.0 40.0 70-71 19.591492842120235 26.0 2.0 36.0 2.0 39.5 72-73 19.195396401247677 23.0 2.0 36.0 2.0 39.0 74-75 18.855679058756593 19.0 2.0 35.0 2.0 39.0 76-77 18.229367119474105 9.0 2.0 35.0 2.0 37.0 78-79 18.185043130162665 2.0 2.0 35.0 2.0 37.0 80-81 17.905078081342776 2.0 2.0 35.0 2.0 36.5 82-83 17.583070014122715 2.0 2.0 35.0 2.0 36.0 84-85 17.25140465135182 2.0 2.0 35.0 2.0 36.0 86-87 16.949371589973886 2.0 2.0 35.0 2.0 35.0 88-89 16.6435538441218 2.0 2.0 35.0 2.0 35.0 90-91 16.33098462757689 2.0 2.0 35.0 2.0 35.0 92-93 16.045924225028703 2.0 2.0 35.0 2.0 35.0 94-95 15.757399185149813 2.0 2.0 35.0 2.0 35.0 96-97 15.475727218231512 2.0 2.0 35.0 2.0 35.0 98-99 15.173602714812596 2.0 2.0 35.0 2.0 35.0 100-101 14.792959978866728 2.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.17481232608281516 1101 2 0.006283342003683856 1101 3 0.12108388954999327 1101 4 -0.11096586210614845 1101 5 0.14907133667877304 1101 6 0.17579821937727758 1101 7 -0.05956447775469087 1101 8 -0.10665732193245958 1101 9 -0.5141007772634936 1101 10-11 -0.39293388229199167 1101 12-13 -0.39379828877763856 1101 14-15 -0.362980289327389 1101 16-17 -0.01591934043891996 1101 18-19 -0.3606390535752979 1101 20-21 -0.5428849231583399 1101 22-23 -0.18958509466291673 1101 24-25 -0.3539525133158534 1101 26-27 -0.43866873656226346 1101 28-29 -0.7067628885868871 1101 30-31 -1.0209243078511534 1101 32-33 -0.9099032235914635 1101 34-35 -1.6533435586242078 1101 36-37 -0.36270792233423066 1101 38-39 -0.4806090017104978 1101 40-41 -1.1204619279947678 1101 42-43 0.10132843306520556 1101 44-45 0.6000360746909976 1101 46-47 -0.23243462129792292 1101 48-49 1.0297173889777227 1101 50-51 0.4927916203610785 1101 52-53 0.30596821703817056 1101 54-55 -0.0843804691786012 1101 56-57 -0.012962909694852698 1101 58-59 -0.4712721917256637 1101 60-61 0.227690457492276 1101 62-63 0.6658653505815728 1101 64-65 -0.3130393643608578 1101 66-67 -0.24501584966177603 1101 68-69 -0.7071229146121176 1101 70-71 -0.7409373671516768 1101 72-73 -0.2055502975236294 1101 74-75 0.10257049739183444 1101 76-77 0.06042334601730914 1101 78-79 -0.1050567103739688 1101 80-81 -0.44308751024938786 1101 82-83 0.1581582836226012 1101 84-85 0.3338258684249311 1101 86-87 0.3635714458522905 1101 88-89 0.5282225381696826 1101 90-91 0.4739460548378922 1101 92-93 -0.6293842556331342 1101 94-95 -1.081460198844857 1101 96-97 -0.8721939099317613 1101 98-99 -0.8127551102520467 1101 100-101 -0.7344185835442865 1102 1 0.09914178997656364 1102 2 -0.12360869578309064 1102 3 -0.20010369479548729 1102 4 0.10866976542421725 1102 5 0.1048743056396404 1102 6 -0.2057807279911401 1102 7 -0.06293829691797725 1102 8 -0.007129656615326496 1102 9 0.13029868292543512 1102 10-11 -0.553696365422887 1102 12-13 -0.07733405126077031 1102 14-15 0.25088610743373607 1102 16-17 0.17976217103207404 1102 18-19 0.21594664143145081 1102 20-21 0.3397061699590651 1102 22-23 0.052486430303346765 1102 24-25 0.1728693490323181 1102 26-27 -0.011880612405718693 1102 28-29 0.18544358914590475 1102 30-31 0.38545221036746824 1102 32-33 1.061081931604214 1102 34-35 1.1745916640478526 1102 36-37 0.7924877591772415 1102 38-39 0.46861501988194476 1102 40-41 0.47569191815907885 1102 42-43 -0.8003247383248109 1102 44-45 -1.8203417930552952 1102 46-47 -0.630882664482808 1102 48-49 -0.7391759983367194 1102 50-51 -1.1079168816632148 1102 52-53 -0.056042579183152696 1102 54-55 -0.1284088092595752 1102 56-57 -0.2565526532845972 1102 58-59 -0.2976891957742467 1102 60-61 -0.061277153843754206 1102 62-63 0.6921811400552578 1102 64-65 0.053188705814584125 1102 66-67 -0.20301180107878025 1102 68-69 0.1707448316766822 1102 70-71 0.22110716726127677 1102 72-73 0.5788626579419578 1102 74-75 0.9520981627089746 1102 76-77 0.5231426442629221 1102 78-79 0.6589446391536917 1102 80-81 0.5936871186305019 1102 82-83 -0.012050893165522325 1102 84-85 -0.9671188009407885 1102 86-87 -0.8216512262124844 1102 88-89 -0.9685723336251897 1102 90-91 -1.6490296536641296 1102 92-93 -1.7737837158220682 1102 94-95 -1.617222681975763 1102 96-97 -2.1110603066928935 1102 98-99 -1.7243610481737743 1102 100-101 -1.555774858847931 1103 1 0.08439820023297528 1103 2 -0.40950613168052996 1103 3 0.16976810007631116 1103 4 0.02982361157806679 1103 5 -0.074612873847542 1103 6 0.1442192720088613 1103 7 0.1713565748768886 1103 8 0.11242162543595668 1103 9 0.17997817010492412 1103 10-11 0.20607927560275385 1103 12-13 0.34205697438024885 1103 14-15 0.4215591843568163 1103 16-17 0.3156596069295077 1103 18-19 0.03245305168785961 1103 20-21 -0.009332291579397634 1103 22-23 0.31370437902129567 1103 24-25 0.18585011826309028 1103 26-27 -0.3626818944569976 1103 28-29 -0.04196025700794337 1103 30-31 0.8465099026751588 1103 32-33 0.7897678290401089 1103 34-35 -0.29511987441367893 1103 36-37 -0.5350763433868551 1103 38-39 -0.49837215960523196 1103 40-41 -0.10171192799477069 1103 42-43 0.5056047488546724 1103 44-45 0.7927992325857289 1103 46-47 1.8405916944915468 1103 48-49 0.9116252837145637 1103 50-51 0.82141004141371 1103 52-53 1.1687971644065946 1103 54-55 1.5422642676635014 1103 56-57 0.5807870903051473 1103 58-59 0.6984646503795986 1103 60-61 0.7517036153870116 1103 62-63 0.5609311400552563 1103 64-65 1.3823553724812498 1103 66-67 1.168142045075065 1103 68-69 1.3984691906510385 1103 70-71 1.8100494749535834 1103 72-73 1.8928049656342658 1103 74-75 2.1403994447602557 1103 76-77 2.0502259775962592 1103 78-79 1.7034959212049792 1103 80-81 1.128623016066399 1103 82-83 1.2325003888857573 1103 84-85 1.6219837631617722 1103 86-87 1.7773872353259783 1103 88-89 2.115722538169681 1103 90-91 2.3778934232589464 1103 92-93 2.2827867969984474 1103 94-95 2.248802959049881 1103 96-97 2.3245166163840327 1103 98-99 2.422113310800585 1103 100-101 2.2326866796136073 1104 1 0.040262397763839886 1104 2 -0.07802465019904758 1104 3 -0.08424424560270438 1104 4 0.1400087967632473 1104 5 -0.18572398495865627 1104 6 0.1275526053421956 1104 7 -0.48743972141940617 1104 8 -0.052238868391199844 1104 9 -0.5862255335987783 1104 10-11 -0.178102823162682 1104 12-13 -0.34490290216296415 1104 14-15 -0.01786982798886072 1104 16-17 -0.25779718319395073 1104 18-19 -0.3583648495467102 1104 20-21 -0.2854125384929773 1104 22-23 -0.08830179381821068 1104 24-25 -0.23073938790975035 1104 26-27 -0.6210923882841684 1104 28-29 -0.3551547014523848 1104 30-31 -0.524091949176686 1104 32-33 -0.5970377265154454 1104 34-35 -0.49349950404331366 1104 36-37 -1.6063726396831584 1104 38-39 -1.0997610484941234 1104 40-41 -0.885894026760198 1104 42-43 -0.39956500423173935 1104 44-45 -0.22679953284636767 1104 46-47 -0.9601799104467226 1104 48-49 -1.2873716298656817 1104 50-51 -0.43175353883320255 1104 52-53 -0.6025762923835316 1104 54-55 -0.7798807940648942 1104 56-57 -0.21049377389238444 1104 58-59 -0.5749921397438555 1104 60-61 0.20178077588084165 1104 62-63 -0.5578188599447422 1104 64-65 0.016151668777546035 1104 66-67 0.1616605635935855 1104 68-69 0.998854993120176 1104 70-71 1.1460834255708683 1104 72-73 0.9668018792145112 1104 74-75 0.6909395682170469 1104 76-77 0.6651179529048967 1104 78-79 0.9101317236741124 1104 80-81 1.0312464728565232 1104 82-83 1.112592981478354 1104 84-85 0.7420454915568371 1104 86-87 1.0719860007580735 1104 88-89 1.3106299455770873 1104 90-91 1.344560089925615 1104 92-93 1.2915059328009129 1104 94-95 1.7923214775683984 1104 96-97 1.6237450114457577 1104 98-99 1.7920207182079881 1104 100-101 2.164785445045709 1105 1 0.03439820023297102 1105 2 0.09049386831947004 1105 3 0.19476810007630974 1105 4 -0.04517638842193605 1105 5 0.21288712615245942 1105 6 0.04421927200885989 1105 7 0.2588565748768943 1105 8 0.22492162543596095 1105 9 -0.18252182989507304 1105 10-11 0.2935792756027453 1105 12-13 -0.25794302561974547 1105 14-15 -0.46594081564318657 1105 16-17 -0.146840393070498 1105 18-19 -0.14879694831213897 1105 20-21 0.29066770842060663 1105 22-23 0.33870437902130135 1105 24-25 -0.00789988173691114 1105 26-27 0.28106810554299955 1105 28-29 0.7017897429920552 1105 30-31 -0.6722400973248384 1105 32-33 -0.4289821709598911 1105 34-35 0.3361301255863225 1105 36-37 0.8586736566131421 1105 38-39 0.3391278403947595 1105 40-41 1.0607880720052378 1105 42-43 0.8368547488546767 1105 44-45 1.0990492325857275 1105 46-47 0.3968416944915525 1105 48-49 0.04912528371456659 1105 50-51 0.37141004141370715 1105 52-53 -0.08120283559340535 1105 54-55 0.19851426766350144 1105 56-57 0.1557870903051466 1105 58-59 0.3422146503795993 1105 60-61 0.5642036153870151 1105 62-63 0.9234311400552606 1105 64-65 0.7198553724812484 1105 66-67 -0.16935795492493355 1105 68-69 -0.5390308093489615 1105 70-71 -0.40870052504641663 1105 72-73 -0.9259450343657356 1105 74-75 -1.1533505552397436 1105 76-77 -0.9997740224037415 1105 78-79 -1.1465040787950223 1105 80-81 -0.9151269839336003 1105 82-83 -1.436249611114242 1105 84-85 -1.4342662368382264 1105 86-87 -1.2663627646740245 1105 88-89 -1.1092774618303167 1105 90-91 -0.7846065767410533 1105 92-93 -0.379713203001554 1105 94-95 -0.8324470409501199 1105 96-97 -1.2379833836159708 1105 98-99 -1.2653866891994152 1105 100-101 -1.0985633203863934 1106 1 0.2645002410492978 1106 2 0.15477958260518676 1106 3 0.1866048347701863 1106 4 0.5160481013739826 1106 5 0.29273406492796994 1106 6 0.3146274352741685 1106 7 0.24763208508097279 1106 8 -0.21487429293139115 1106 9 -0.015174891119563938 1106 10-11 0.01590070417417877 1106 12-13 -0.08230527051770764 1106 14-15 0.179850000683345 1106 16-17 0.2840269538682847 1106 18-19 0.039468357810307 1106 20-21 0.1281677084206052 1106 22-23 0.3481431545315061 1106 24-25 0.41658991418145774 1106 26-27 0.1703538198287049 1106 28-29 0.2627591307471633 1106 30-31 0.49102520879761613 1106 32-33 0.3258647678156237 1106 34-35 0.3963342072189704 1106 36-37 -0.5804845066521622 1106 38-39 -0.9404639963399362 1106 40-41 -0.5031149892192559 1106 42-43 -0.7162064756351185 1106 44-45 -2.0447007674142696 1106 46-47 -1.608642999386003 1106 48-49 -2.487736961183394 1106 50-51 -1.9348399585862914 1106 52-53 -1.664493651919937 1106 54-55 -1.6076081813160918 1106 56-57 -1.1047996443887307 1106 58-59 -0.859698614926522 1106 60-61 -0.960413731551764 1106 62-63 -1.6088392681080101 1106 64-65 -1.3566752397636463 1106 66-67 -0.9078783630881979 1106 68-69 -1.42946448281835 1106 70-71 -1.5171188923933556 1106 72-73 -1.4246695241616543 1106 74-75 -1.4138097389132156 1106 76-77 -1.3220954509751728 1106 78-79 -0.8294122420603323 1106 80-81 -0.5037749431172749 1106 82-83 -0.8371424682570954 1106 84-85 -1.1836284817361857 1106 86-87 -1.2325617442658618 1106 88-89 -1.2651448087690937 1106 90-91 -1.0925147400063597 1106 92-93 -0.6538203458586977 1106 94-95 -0.7568092858480799 1106 96-97 -0.09614664892209213 1106 98-99 0.027470453657725713 1106 100-101 0.09493157757279036 1107 1 0.20075622492433354 1107 2 -0.040987613162009495 1107 3 0.17501501365655514 1107 4 -0.05752206743428445 1107 5 0.11057231133764134 1107 6 0.06582421028046781 1107 7 -0.018303918950266507 1107 8 0.1329463167939835 1107 9 -0.1911638052037148 1107 10-11 -0.4497077614342899 1107 12-13 -0.23379179105184988 1107 14-15 0.2043523942333607 1107 16-17 0.09405466865789691 1107 18-19 0.21570922452736596 1107 20-21 0.26397017755640917 1107 22-23 -0.22410426295400754 1107 24-25 0.3680260441890155 1107 26-27 0.2554508215923761 1107 28-29 0.5213885084241596 1107 30-31 0.019117927366522736 1107 32-33 0.007900545089491118 1107 34-35 -0.41325259046305973 1107 36-37 -0.41501461499179726 1107 38-39 -0.06272401145708528 1107 40-41 -0.027869335402172624 1107 42-43 -0.36252796719470126 1107 44-45 0.21764491159807164 1107 46-47 -0.33055028081708926 1107 48-49 -1.58984076566815 1107 50-51 -1.0305189709319684 1107 52-53 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337.0 11 647.0 12 1200.0 13 1627.0 14 2301.0 15 3177.0 16 4823.0 17 7999.0 18 7808.0 19 3781.0 20 1973.0 21 1732.0 22 2019.0 23 2194.0 24 2434.0 25 2723.0 26 2150.0 27 1250.0 28 1112.0 29 1299.0 30 1454.0 31 1604.0 32 1709.0 33 2093.0 34 2553.0 35 4269.0 36 5133.0 37 9218.0 38 14047.0 39 3457.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.811354883290825 18.563720060564798 15.797656670765292 13.827268385379089 2 18.593215000558814 21.941009723337025 41.910935452079286 17.554839824024874 3 19.11603332655964 33.83966673440358 36.11867506604349 10.925624872993293 4 10.90831487164895 26.404951118879698 31.199569114448895 31.487164895022456 5 7.36718043546732 39.41558375582943 34.01948731495687 19.19774849374638 6 18.290440242626214 40.61855460613881 17.07324507483007 24.017760076404908 7 16.31630817999858 45.935401278156526 18.374770124869187 19.373520416975705 8 26.526320067463903 32.05348343375024 17.94194446420044 23.478252034585413 9 28.273879072980908 13.888013980472044 18.03033843715392 39.807768509393135 10-11 23.154140800422667 30.082907450494297 20.959023805411338 25.803927943671702 12-13 30.191113865661485 30.50354083903153 24.59079686658606 14.714548428720928 14-15 26.17630025502169 27.30510144986436 30.382126129055198 16.136472166058745 16-17 14.916380484038122 33.105403263498005 31.548840706346144 20.429375546117736 18-19 13.863121424870203 21.978602562409193 46.0624041128598 18.095871899860803 20-21 17.299818131940707 18.422015179378803 48.893551304065106 15.384615384615385 22-23 16.24417056988712 21.781494162949716 49.264399581398656 12.709935685764506 24-25 15.160582384198815 23.614399073387318 49.69570120805096 11.529317334362903 26-27 13.591335358605203 24.56387226562897 50.53036383772086 11.31442853804497 28-29 12.516193930895733 23.064221954651913 50.914226781078376 13.505357333373977 30-31 14.31067941436453 23.35480527925383 50.56643264277658 11.768082663605052 32-33 13.320057303679016 23.940034341566506 50.80977007406805 11.930138280686425 34-35 13.09982878365265 22.539082544569595 50.88783551037205 13.473253161405701 36-37 12.366703076180558 25.52493204968628 49.29915919424899 12.809205679884167 38-39 12.759212785629376 22.745699684016948 50.29413856517277 14.200948965180904 40-41 12.799853692734423 22.63088912144519 50.397772878290645 14.171484307529743 42-43 14.415837761498837 22.707090822267155 49.204962254757525 13.672109161476484 44-45 13.221503103949281 25.12319274966217 46.725866921349684 14.929437225038864 46-47 16.436198855958466 24.36930392286356 41.958688517927726 17.235808703250257 48-49 17.273909553661237 25.319285126444026 38.93856110868394 18.468244211210795 50-51 16.947766274143238 27.352854515712792 37.38963453664286 18.309744673501115 52-53 16.43721487863609 28.908385235158452 37.79197951698282 16.86242036922264 54-55 16.169492903081597 29.982321205409306 35.057354480152 18.7908314113571 56-57 17.650345955721733 31.406276988102373 32.6305843146419 18.31279274153399 58-59 15.224591812889262 33.15434400495819 35.418042530709286 16.20302165144326 60-61 16.56421459053038 35.24893314366999 31.986384881121722 16.20046738467791 62-63 14.69433168555766 36.916441007768086 31.826287525847047 16.562939780827207 64-65 14.562440942481789 37.18566159661048 31.61215594232938 16.63974151857835 66-67 14.671753863661765 37.18260056697522 30.942316876149444 17.20332869321357 68-69 14.876625059058417 36.76456393296044 30.985221424616054 17.373589583365085 70-71 15.024435345396908 36.033752273350736 31.211708645336966 17.730103735915385 72-73 14.79978052572218 35.491327717772336 31.006838249489416 18.702053507016064 74-75 14.884245500129548 35.11194427933489 31.13813827544338 18.86567194509218 76-77 14.452996012090733 35.29579110467627 30.93499961898956 19.31621326424344 78-79 14.460616220884454 35.111890065787804 30.44324214483477 19.98425156849298 80-81 14.15943140771298 35.10518855702942 30.402820607914162 20.332559427343437 82-83 14.147089904080637 35.042574378149894 30.37971468053975 20.430621037229717 84-85 14.22497002702648 34.920546219341205 29.998374347198798 20.85610940643352 86-87 14.051736400398285 34.99979679339985 29.53201519985369 21.416451606348176 88-89 14.048108918184358 34.73646777921715 29.409941832406204 21.805481470192284 90-91 14.089186470642026 34.5564553000823 29.355943224652776 21.998415004622903 92-93 13.94795931845199 34.24860042876157 29.635349460999972 22.168090791786472 94-95 13.979964032797213 33.92804527397051 29.770480477124252 22.321510216108024 96-97 13.9250988082054 34.24301230403463 29.22690834459425 22.604980543165723 98-99 14.189772715727015 34.061144244739545 28.870284384747467 22.878798654785975 100-101 14.062404746906179 34.21033915181565 28.296519070939425 23.430737030338744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 688.0 1 411.5 2 141.0 3 129.0 4 96.5 5 96.5 6 173.5 7 321.0 8 467.0 9 563.5 10 528.5 11 460.0 12 498.5 13 578.5 14 671.5 15 766.5 16 839.0 17 959.0 18 1102.5 19 1220.5 20 1414.5 21 1556.0 22 1658.5 23 1821.0 24 1978.5 25 2127.5 26 2318.5 27 2518.0 28 2694.5 29 2844.0 30 2956.0 31 3121.5 32 3179.0 33 3194.5 34 3267.5 35 3310.0 36 3357.0 37 3309.5 38 3356.5 39 3399.0 40 3286.0 41 3157.5 42 3040.5 43 2938.0 44 2740.5 45 2461.5 46 2230.0 47 2037.0 48 1974.0 49 1891.5 50 1772.0 51 1510.0 52 1089.5 53 850.5 54 702.0 55 598.0 56 512.5 57 428.5 58 342.5 59 271.0 60 201.0 61 163.5 62 133.5 63 96.5 64 69.0 65 40.0 66 37.0 67 32.0 68 19.0 69 14.5 70 11.0 71 8.0 72 5.0 73 3.0 74 1.0 75 1.5 76 1.5 77 0.5 78 0.5 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016256362842018635 2 0.0 3 0.003048068032878494 4 0.021336476230149455 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0010160226776261647 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.00660414740457007 30-31 0.0 32-33 0.0 34-35 0.009652215437448564 36-37 0.005588124726943905 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.003048068032878494 62-63 0.007620170082196235 64-65 0.0020320453552523294 66-67 0.006096136065756988 68-69 0.0035560793716915757 70-71 0.0 72-73 0.006096136065756988 74-75 0.0035560793716915757 76-77 5.080113388130824E-4 78-79 5.080113388130824E-4 80-81 0.004572102049317741 82-83 0.007112158743383151 84-85 0.0010160226776261647 86-87 0.0010160226776261647 88-89 5.080113388130824E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0010160226776261647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 98423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.19699663696494 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95257127631228 49.16228930229723 2 9.677591260325073 11.070583095414689 3 2.14228617106315 3.675970047651463 4 0.8171240785149658 1.869481726832143 5 0.33040234479083397 0.944901090192333 6 0.18118838262723155 0.6218058787072127 7 0.13855582200905942 0.5547483819838859 8 0.08881783462119194 0.40640907105046586 9 0.07105426769695354 0.3657681639454193 >10 0.46718181010746956 5.079097365453197 >50 0.05329070077271516 2.1641283033437304 >100 0.0639488409272582 7.2950428253558615 >500 0.0053290700772715156 2.2047692104487773 >1k 0.010658140154543031 14.585005537323593 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3793 3.8537740162360423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3394 3.448380967863203 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 3143 3.1933592757790357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1647 1.673389350050293 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1238 1.2578360749011919 RNA PCR Primer, Index 33 (95% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1140 1.1582658524938276 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 911 0.925596659317436 RNA PCR Primer, Index 33 (95% over 24bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 719 0.7305203052132124 RNA PCR Primer, Index 33 (96% over 29bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 540 0.5486522459181289 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.4399378194121293 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 433 0.4399378194121293 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 390 0.3962488442742042 RNA PCR Primer, Index 33 (96% over 27bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 383 0.38913668553082104 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 362 0.3678002093006716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 307 0.31191896203123254 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.2926145311563354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 282 0.2865183950905784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 266 0.27026203224855977 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 254 0.25806976011704585 RNA PCR Primer, Index 15 (95% over 24bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 232 0.2357172612092702 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 206 0.20930067159098992 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 200 0.20320453552523293 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 194 0.19710839945947592 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 186 0.18898021803846662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 186 0.18898021803846662 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 178 0.1808520366174573 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.17475590055170032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 159 0.1615476057425602 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 146 0.14833931093342004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 144 0.1463072655781677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 143 0.14529124290054155 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.13411499344665373 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 131 0.13309897076902755 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 131 0.13309897076902755 RNA PCR Primer, Index 33 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 126 0.12801885738089674 No Hit CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 120 0.12192272131513976 RNA PCR Primer, Index 33 (96% over 29bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 119 0.12090669863751358 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 116 0.1178586306046351 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 116 0.1178586306046351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 113 0.1148105625717566 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 112 0.11379453989413042 No Hit ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 109 0.11074647186125194 RNA PCR Primer, Index 15 (96% over 25bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 106 0.10769840382837345 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 104 0.10566635847312111 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 101 0.10261829044024262 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0010160226776261647 0.0 5 0.0 0.0 0.0 0.0010160226776261647 0.0 6 0.0 0.0 0.0 0.0010160226776261647 0.0 7 0.0 0.0 0.0 0.0010160226776261647 0.0 8 0.0 0.0 0.0 0.0010160226776261647 0.0 9 0.0 0.0 0.0 0.0010160226776261647 0.0 10-11 0.0 0.0 0.0 0.0010160226776261647 0.0 12-13 0.0 0.0 0.0 0.0010160226776261647 0.0 14-15 0.0 0.0 0.0 0.0015240340164392472 0.0 16-17 0.0 0.0 0.0 0.0020320453552523294 0.0 18-19 0.0 0.0 0.0 0.0020320453552523294 0.0 20-21 0.0 0.0 0.0 0.004064090710504659 0.0 22-23 0.0 0.0 0.0 0.0086361927598224 0.0 24-25 0.0 0.0 0.0 0.012192272131513974 0.0 26-27 0.0 0.0 0.0 0.020320453552523293 0.0 28-29 0.0 0.0 0.0 0.04826107718724282 0.0 30-31 0.0 0.0 0.0 0.08178982554890625 0.0 32-33 0.0 0.0 0.0 0.14021112951241071 0.0 34-35 0.0 0.0 0.0 0.24587748798553183 0.0 36-37 0.0 0.0 0.0 0.37897645875455943 0.0 38-39 0.0 0.0 0.0 0.48870690793818516 0.0 40-41 0.0 0.0 0.0 0.5684646881318391 0.0 42-43 0.0 0.0 0.0 0.66092275179582 0.0 44-45 0.0 0.0 0.0 0.783861495788586 0.0 46-47 0.0 0.0 0.0 0.919500523251679 0.0 48-49 0.0 0.0 0.0 1.0780000609613607 0.0 50-51 0.0 0.0 0.0 1.191794600855491 0.0 52-53 0.0 0.0 0.0 1.3259095943021448 0.0 54-55 0.0 0.0 0.0 1.4839011206730133 0.0 56-57 0.0 0.0 0.0 1.6871056561982463 0.0 58-59 0.0 0.0 0.0 1.8557654206841896 0.0 60-61 0.0 0.0 0.0 2.0335693892687687 0.0 62-63 0.0 0.0 0.0 2.2352498907775624 0.0 64-65 0.0 0.0 0.0 2.465379027259888 0.0 66-67 0.0 0.0 0.0 2.7427532182518313 0.0 68-69 0.0 0.0 0.0 3.069912520447456 0.0 70-71 0.0 0.0 0.0 3.3843715391727542 0.0 72-73 0.0 0.0 0.0 3.6622537415035104 0.0 74-75 0.0 0.0 0.0 3.968584578807799 0.0 76-77 0.0 0.0 0.0 4.2825355861942835 0.0 78-79 0.0 0.0 0.0 4.6447476707680115 0.0 80-81 0.0 0.0 0.0 4.993751460532599 0.0 82-83 0.0 0.0 0.0 5.371711896609533 0.0 84-85 0.0 0.0 0.0 5.670930575170438 0.0 86-87 0.0 0.0 0.0 6.034158682421792 0.0 88-89 0.0 0.0 0.0 6.486288773965435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1610 0.0 63.463585 1 GTGGTAT 475 0.0 48.024143 1 TGGTATC 505 0.0 45.14827 2 GTATCAA 2790 0.0 39.518078 1 GGGGTAT 65 1.3194187E-4 36.53827 7 ATTGATA 55 0.002568294 34.54527 2 TGGGGTA 85 1.6172067E-5 33.529232 6 TATCAAC 3520 0.0 31.576534 2 ATGGGCT 140 1.6727427E-8 30.535551 5 ATCAACG 3665 0.0 30.327257 3 TCAACGC 3755 0.0 29.600372 4 CAACGCA 3820 0.0 28.972357 5 AACGCAG 3920 0.0 28.596788 6 TGGGCAG 85 6.3655106E-4 27.941027 6 ATGGGTA 110 9.390923E-5 25.90895 5 CTTATAC 1105 0.0 25.374748 1 ACGCAGA 4410 0.0 25.311657 7 CATGGGC 320 0.0 25.234241 4 CGCAGAG 4455 0.0 25.162603 8 GAGTACT 3250 0.0 24.84602 12-13 >>END_MODULE