FastQCFastQC Report
Fri 10 Feb 2017
SRR2031683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709067
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.3857075234616314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.32789820410785536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32500.19016223471636864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29340.17167261435625403No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.11754951678313373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.063.4820371
GTATCAA60300.040.727751
TGGTATC12550.034.065592
GTGGTAT13150.033.594981
TCAACGC74150.032.992354
ATCAACG75100.032.5750053
CAACGCA75950.032.083475
TATCAAC77150.032.078862
AACGCAG78100.031.3191366
ACGCAGA85650.028.336567
CGCAGAG88350.027.4208458
GCAGAGT95850.024.6805259
GAGTACT60750.023.14834212-13
CAGAGTA90950.023.0609410-11
GTACTTT65000.021.48892814-15
GTACATG52200.021.4762611
TACATGG53200.020.626122
AGAGTAC86850.020.59416210-11
CATGGGG30550.019.902884
ACATGGG52350.019.5999183
Produced by FastQC (version 0.11.5)