##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031683_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1709067 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12002747698013 33.0 31.0 34.0 31.0 34.0 2 32.423789120028644 34.0 31.0 34.0 31.0 34.0 3 32.52627954316595 34.0 31.0 34.0 31.0 34.0 4 35.963436775737875 37.0 37.0 37.0 35.0 37.0 5 35.9673418303671 37.0 37.0 37.0 35.0 37.0 6 36.003735371404396 37.0 37.0 37.0 35.0 37.0 7 35.98690513596015 37.0 37.0 37.0 35.0 37.0 8 35.98173857432155 37.0 37.0 37.0 35.0 37.0 9 37.780301766987485 39.0 39.0 39.0 35.0 39.0 10-11 37.714560049430474 39.0 38.0 39.0 35.0 39.0 12-13 37.663206006552116 39.0 38.0 39.0 35.0 39.0 14-15 39.07840271914442 41.0 39.0 41.0 36.0 41.0 16-17 38.99995991965207 41.0 39.0 41.0 36.0 41.0 18-19 39.01090799834061 41.0 39.0 41.0 36.0 41.0 20-21 39.01886584902756 41.0 39.0 41.0 36.0 41.0 22-23 38.92109466744136 41.0 39.0 41.0 36.0 41.0 24-25 38.85045027491608 41.0 39.0 41.0 35.0 41.0 26-27 38.730368674838374 41.0 39.0 41.0 35.0 41.0 28-29 38.61243795591396 40.0 38.5 41.0 35.0 41.0 30-31 38.4341582278518 40.0 38.0 41.0 35.0 41.0 32-33 38.21487308572455 40.0 38.0 41.0 34.0 41.0 34-35 38.03337259452087 40.0 38.0 41.0 34.0 41.0 36-37 37.861092923799944 40.0 38.0 41.0 33.5 41.0 38-39 37.66674653480525 40.0 38.0 41.0 33.0 41.0 40-41 37.555737428667214 40.0 38.0 41.0 33.0 41.0 42-43 37.499032220503935 40.0 38.0 41.0 33.0 41.0 44-45 37.32837477992378 40.0 37.5 41.0 32.5 41.0 46-47 37.19142432684032 40.0 37.5 41.0 32.0 41.0 48-49 37.18223305464326 40.0 38.0 41.0 32.0 41.0 50-51 36.61353299782864 39.5 36.5 40.5 31.5 41.0 52-53 36.62182611916326 39.5 37.0 40.5 31.0 41.0 54-55 36.82411192773601 40.0 37.0 41.0 31.5 41.0 56-57 36.679108542848226 40.0 36.0 41.0 31.0 41.0 58-59 36.47516100890135 40.0 36.0 41.0 31.0 41.0 60-61 36.22853609601028 39.0 35.0 41.0 31.0 41.0 62-63 35.93717215299341 39.0 35.0 41.0 30.0 41.0 64-65 35.649674939601546 39.0 35.0 41.0 30.0 41.0 66-67 35.34669852030377 38.0 35.0 40.5 29.0 41.0 68-69 35.00321608222498 37.0 35.0 40.0 29.0 41.0 70-71 34.64491444747338 37.0 35.0 39.5 29.0 41.0 72-73 34.27474025301524 36.0 35.0 39.0 28.0 41.0 74-75 33.87609994224919 36.0 34.0 39.0 27.5 41.0 76-77 33.507950829312136 35.0 34.0 37.5 27.0 40.0 78-79 33.13330665210901 35.0 34.0 37.0 26.5 39.0 80-81 32.79485912489095 35.0 34.0 37.0 26.0 39.0 82-83 32.477701868914444 35.0 34.0 36.0 26.0 38.0 84-85 32.16136874680747 35.0 34.0 36.0 25.0 37.0 86-87 31.897422687349298 35.0 34.0 35.5 25.0 37.0 88-89 31.631380747507265 35.0 33.0 35.0 24.0 36.5 90-91 31.396234319660962 35.0 33.0 35.0 23.0 36.0 92-93 31.188007257761107 35.0 33.0 35.0 20.5 36.0 94-95 30.94559897300691 35.0 33.0 35.0 19.5 36.0 96-97 30.689011899474977 35.0 33.0 35.0 16.5 35.0 98-99 30.44305430974912 35.0 32.0 35.0 2.0 35.0 100-101 29.85998998283859 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0970200806247945 1101 2 -0.04002000471262335 1101 3 0.11146542591524167 1101 4 0.13825177223396423 1101 5 0.011451257859576458 1101 6 0.07672838458326936 1101 7 0.10187967112400287 1101 8 0.12363855758871267 1101 9 0.1910682660757672 1101 10-11 0.09599916710528333 1101 12-13 0.09369106632979651 1101 14-15 0.030908850737922933 1101 16-17 0.07101563735472638 1101 18-19 0.10475791450986804 1101 20-21 0.054606552708214906 1101 22-23 0.0758362121357834 1101 24-25 0.047974716355334124 1101 26-27 -0.05375758241810047 1101 28-29 -0.10807156774141902 1101 30-31 0.07651726312843721 1101 32-33 -0.15927369416295534 1101 34-35 -0.10311101345743623 1101 36-37 -0.06788888927572856 1101 38-39 0.07712991499585797 1101 40-41 -0.07279321316666199 1101 42-43 -0.15786202369201163 1101 44-45 -0.36676565010307627 1101 46-47 -0.22031310677694904 1101 48-49 -0.03278843617297156 1101 50-51 0.17153851267115527 1101 52-53 0.1868378785758793 1101 54-55 0.2754951714538052 1101 56-57 0.05314625715948296 1101 58-59 -0.13901773645492455 1101 60-61 -0.1534117077832704 1101 62-63 -0.05138414196026275 1101 64-65 -0.012500966927994739 1101 66-67 -0.2135026997684193 1101 68-69 -0.3840441790044409 1101 70-71 -0.29757917216638674 1101 72-73 -0.12050352719305835 1101 74-75 -0.13253541003398794 1101 76-77 -0.3052545778734199 1101 78-79 -0.3869344969332573 1101 80-81 -0.44494739835590025 1101 82-83 -0.2528067118257269 1101 84-85 -0.3169374035982244 1101 86-87 -0.42734491104167915 1101 88-89 -0.5163457298267069 1101 90-91 -0.8534300594372475 1101 92-93 -0.6448371718972865 1101 94-95 -0.5592355852437301 1101 96-97 -0.6174822042801189 1101 98-99 -0.57527345646103 1101 100-101 -0.5182941777521854 1102 1 -0.10368495130264677 1102 2 0.1028044724246584 1102 3 0.1736942480313104 1102 4 0.26223238431570906 1102 5 0.2697118207925584 1102 6 0.20372100131707072 1102 7 0.10876312678169597 1102 8 0.058803799510592114 1102 9 0.15588703547299332 1102 10-11 0.20493902995306712 1102 12-13 0.21092795944436915 1102 14-15 0.47977748729768877 1102 16-17 0.33154944027342736 1102 18-19 0.3748426545185879 1102 20-21 0.2847636141200809 1102 22-23 0.2897292807140843 1102 24-25 0.1429089327895099 1102 26-27 0.2910124762581603 1102 28-29 0.15936737411454516 1102 30-31 0.043441938919599465 1102 32-33 0.15759567831417343 1102 34-35 0.2318323581392221 1102 36-37 0.11594042169974017 1102 38-39 0.1382945042291297 1102 40-41 0.1357245923102255 1102 42-43 0.2991509840854931 1102 44-45 0.029014433257088967 1102 46-47 0.08046552008008945 1102 48-49 0.32485886228750616 1102 50-51 0.18829662145113701 1102 52-53 0.039636007243139204 1102 54-55 0.11417091443466632 1102 56-57 0.25741085449660517 1102 58-59 0.20472225693058022 1102 60-61 0.12453749201609554 1102 62-63 0.25148812600127 1102 64-65 0.2688408995424325 1102 66-67 0.1610211989290491 1102 68-69 0.17112312173387778 1102 70-71 0.012781055004289499 1102 72-73 0.08170816732238961 1102 74-75 -0.2356212254457688 1102 76-77 0.2260418413191232 1102 78-79 0.1818523126240379 1102 80-81 0.1822219699597909 1102 82-83 0.3172447366830582 1102 84-85 0.20551442776280737 1102 86-87 0.20607909782437162 1102 88-89 0.16730398226752996 1102 90-91 0.2915776392221119 1102 92-93 0.30331635448294136 1102 94-95 0.08648174801951214 1102 96-97 -0.12901354757023853 1102 98-99 0.04888390720261171 1102 100-101 -0.11792819027591861 1103 1 -0.022769174071790133 1103 2 0.16161317800876418 1103 3 0.04943777791606152 1103 4 0.02064895663719568 1103 5 0.12492843022523203 1103 6 0.10229627414494757 1103 7 0.06240923894526418 1103 8 0.13256898511462367 1103 9 0.13020425009396774 1103 10-11 0.0985774273345541 1103 12-13 0.2419339862521923 1103 14-15 0.35881275505202836 1103 16-17 0.2854406618460672 1103 18-19 0.24049093651685638 1103 20-21 0.08277319198442257 1103 22-23 0.14643806942224558 1103 24-25 0.10583702274571039 1103 26-27 0.15518863908603464 1103 28-29 0.052173068175378035 1103 30-31 0.15484301606365847 1103 32-33 0.11046717787807836 1103 34-35 0.12943952193707986 1103 36-37 0.13151542162659524 1103 38-39 0.1928103473239986 1103 40-41 0.24846718898216835 1103 42-43 0.3341178368514264 1103 44-45 0.16700998576462212 1103 46-47 0.03153767000930685 1103 48-49 0.3258023141050259 1103 50-51 0.2555855275025607 1103 52-53 0.2514525375219918 1103 54-55 0.17489222566343443 1103 56-57 0.16924522991060797 1103 58-59 0.22473764470286284 1103 60-61 0.14753849209592573 1103 62-63 0.26209281697326503 1103 64-65 0.22808315414778946 1103 66-67 0.12353972840632821 1103 68-69 0.08303690173053013 1103 70-71 0.1021943763349995 1103 72-73 -0.03323560796430769 1103 74-75 0.04747282844654421 1103 76-77 -0.0730263250075609 1103 78-79 -0.060460801377125506 1103 80-81 0.043914858945079516 1103 82-83 0.03320430554124698 1103 84-85 -0.011089363011969056 1103 86-87 0.07754439237984911 1103 88-89 0.034292112118262 1103 90-91 -0.023525655691940983 1103 92-93 -0.41781516917815864 1103 94-95 -0.02101074302343875 1103 96-97 0.0613666708257945 1103 98-99 0.023449365624074403 1103 100-101 -0.16511457247358052 1104 1 -0.008265562248851666 1104 2 0.0842924434520711 1104 3 0.25022919410046995 1104 4 0.08286599282840257 1104 5 0.08435139525561652 1104 6 0.22092937774257138 1104 7 0.09974826563107086 1104 8 0.10172461615143646 1104 9 0.14072103447093554 1104 10-11 0.26726302363138643 1104 12-13 0.32116861215791914 1104 14-15 0.3895002606790783 1104 16-17 0.3935801833553825 1104 18-19 0.3568906710985402 1104 20-21 0.28899672005700694 1104 22-23 0.33850086393762524 1104 24-25 0.3186777642194656 1104 26-27 0.34151670040988336 1104 28-29 0.3075832667909282 1104 30-31 0.3430903231108502 1104 32-33 0.3825998806908686 1104 34-35 0.45529623174149236 1104 36-37 0.5844502731696295 1104 38-39 0.39791163820415676 1104 40-41 0.537491951178545 1104 42-43 0.35973256859925584 1104 44-45 0.5030604326962305 1104 46-47 0.5802023882125624 1104 48-49 0.6964596686554287 1104 50-51 0.5156617658602656 1104 52-53 0.5545234100034051 1104 54-55 0.48202865472411816 1104 56-57 0.48786837833822716 1104 58-59 0.4731165583042838 1104 60-61 0.5063505825110752 1104 62-63 0.4704539302547701 1104 64-65 0.3933883554663211 1104 66-67 0.45467961916916266 1104 68-69 0.41385850468741125 1104 70-71 0.4406100685385894 1104 72-73 0.3645144839524477 1104 74-75 0.3693490567300941 1104 76-77 0.3302919195667755 1104 78-79 0.30892221208853243 1104 80-81 0.2403848984444963 1104 82-83 0.24125823638573962 1104 84-85 0.3779307190646364 1104 86-87 0.2837369840675592 1104 88-89 0.38577465903943775 1104 90-91 0.40132153641468093 1104 92-93 0.2688061928715406 1104 94-95 0.3640121994789105 1104 96-97 0.3222250444524484 1104 98-99 0.24546274521391354 1104 100-101 0.1528119123638234 1105 1 -0.13620602298108153 1105 2 0.014993812064290069 1105 3 -0.03536513170542577 1105 4 -0.039111157123130624 1105 5 0.0018518542153103112 1105 6 0.1781305018866064 1105 7 0.12646251267091912 1105 8 0.14415111091705768 1105 9 0.18059076067029167 1105 10-11 0.11612917906847287 1105 12-13 0.026989598885890587 1105 14-15 0.12607247784726638 1105 16-17 0.19954152051629848 1105 18-19 0.18150625278945398 1105 20-21 0.249282835343962 1105 22-23 0.012867378536270735 1105 24-25 0.1696761737798269 1105 26-27 0.18511515294302683 1105 28-29 0.07594565764138395 1105 30-31 0.1828862061399903 1105 32-33 0.21871384568927965 1105 34-35 0.29182577403673093 1105 36-37 0.18840380596765982 1105 38-39 0.25085499419935076 1105 40-41 0.2766443969216894 1105 42-43 0.1734262129105204 1105 44-45 0.26933711705968477 1105 46-47 0.4085492284286758 1105 48-49 0.35204477058171335 1105 50-51 0.23122238000605222 1105 52-53 0.35304438678878824 1105 54-55 0.24949658619557624 1105 56-57 0.31692125310161856 1105 58-59 0.36882806871550144 1105 60-61 0.4111768648705336 1105 62-63 0.4469598486936306 1105 64-65 0.3993656305378934 1105 66-67 0.3029602053416838 1105 68-69 0.38877714872869973 1105 70-71 0.32586846936289504 1105 72-73 0.05870638161020736 1105 74-75 0.28823123572549036 1105 76-77 0.36251796531528413 1105 78-79 0.45740563424096337 1105 80-81 0.5247883495234902 1105 82-83 0.4771313080568049 1105 84-85 0.4233338420758912 1105 86-87 0.4738027130538143 1105 88-89 0.3919144784569504 1105 90-91 0.37391894292933614 1105 92-93 0.4557750145199897 1105 94-95 0.3614280609025009 1105 96-97 0.4056069424863118 1105 98-99 0.46410946764589056 1105 100-101 0.5954985525591567 1106 1 0.02541860724107181 1106 2 0.003559518507117332 1106 3 0.12120637987919736 1106 4 0.050945875214949865 1106 5 0.11148353271222078 1106 6 0.19391756766641066 1106 7 0.1532838289318903 1106 8 0.1541641132204674 1106 9 0.2401740509396646 1106 10-11 0.22974918223412288 1106 12-13 0.22201547701115487 1106 14-15 0.16584839590456113 1106 16-17 0.2959406991812372 1106 18-19 0.15763340216272326 1106 20-21 0.14987990053833755 1106 22-23 0.2172942689475974 1106 24-25 0.3254781354725438 1106 26-27 0.3429574427361075 1106 28-29 0.18555818321775064 1106 30-31 0.3041261806702167 1106 32-33 0.21050736130541026 1106 34-35 0.20236320498683114 1106 36-37 0.21143483153066 1106 38-39 0.21418184926812245 1106 40-41 0.19262993032329945 1106 42-43 0.2391523507204809 1106 44-45 0.2851409868169412 1106 46-47 0.32792085362986967 1106 48-49 0.35209301425890516 1106 50-51 0.28614590926051875 1106 52-53 0.38804616837177974 1106 54-55 0.3879373288663288 1106 56-57 0.2475665402906273 1106 58-59 0.29368032019550583 1106 60-61 0.11097561465460615 1106 62-63 0.08957466322469543 1106 64-65 0.23544030090891255 1106 66-67 0.32091137045359375 1106 68-69 0.3308551032835183 1106 70-71 0.24977590365934077 1106 72-73 0.14782979941634977 1106 74-75 0.17696469284210536 1106 76-77 0.07672314440954153 1106 78-79 0.18661242868531502 1106 80-81 0.29140820597054073 1106 82-83 0.22348014706458486 1106 84-85 0.2680253391187506 1106 86-87 0.18318677681207873 1106 88-89 0.1340066712206145 1106 90-91 0.23371486675419817 1106 92-93 0.31776575462497547 1106 94-95 0.307097910808654 1106 96-97 0.43816347431883074 1106 98-99 0.4954893596812404 1106 100-101 0.5156321387592264 1107 1 0.015668428504852727 1107 2 0.1897947826115285 1107 3 0.15242222708886288 1107 4 0.24187001232058236 1107 5 0.0763472994953176 1107 6 0.16904961199929147 1107 7 0.10838481883132545 1107 8 0.17805886199766263 1107 9 0.11099107229667737 1107 10-11 0.1248420463284603 1107 12-13 0.17764519541106694 1107 14-15 0.17437183003357148 1107 16-17 0.242633606739183 1107 18-19 0.22205796603060435 1107 20-21 0.14042459719069456 1107 22-23 0.1320075666071716 1107 24-25 0.08382834507421677 1107 26-27 0.12869720595392664 1107 28-29 0.05725628751152101 1107 30-31 0.08750733447155312 1107 32-33 0.19387583822088317 1107 34-35 0.053393261017092186 1107 36-37 0.08986388978764381 1107 38-39 0.20776466791463832 1107 40-41 0.1367173835133073 1107 42-43 0.07785472578623143 1107 44-45 0.11984659960657496 1107 46-47 0.0997483251501805 1107 48-49 0.18933164206868724 1107 50-51 0.1767872966944921 1107 52-53 0.21786842425904496 1107 54-55 0.30196875179558447 1107 56-57 0.33821572282833756 1107 58-59 0.27263708335348724 1107 60-61 0.33741629155306896 1107 62-63 0.30073890527290104 1107 64-65 0.2599173203132068 1107 66-67 0.21546527005265403 1107 68-69 0.40484284213652444 1107 70-71 0.3780805552103317 1107 72-73 0.41700378613160893 1107 74-75 0.32423585536765387 1107 76-77 0.30978532886531696 1107 78-79 0.257186463273527 1107 80-81 0.267141418151688 1107 82-83 0.2588577135434349 1107 84-85 0.35246954034896305 1107 86-87 0.428630184359001 1107 88-89 0.37027154097151893 1107 90-91 0.401583606655997 1107 92-93 0.5108870873829936 1107 94-95 0.5341448735527798 1107 96-97 0.6567098370761855 1107 98-99 0.7274204667119513 1107 100-101 0.5973181330837605 1108 1 -0.08880072277298012 1108 2 -0.05502057402714655 1108 3 0.1016481276310941 1108 4 0.07398060097064274 1108 5 0.1809298236501391 1108 6 0.07619496582059782 1108 7 0.15153393316693098 1108 8 0.12492540140546993 1108 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13 4403.0 14 5249.0 15 6518.0 16 8066.0 17 11472.0 18 10860.0 19 6325.0 20 5499.0 21 5420.0 22 5659.0 23 6059.0 24 6596.0 25 7626.0 26 8961.0 27 10717.0 28 12817.0 29 15765.0 30 19873.0 31 25607.0 32 33388.0 33 45465.0 34 67553.0 35 110306.0 36 192397.0 37 371223.0 38 505335.0 39 174120.0 40 167.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.639047824963484 19.882624610277027 14.130457073783253 23.347870490976238 2 16.546437149485204 21.80807771254725 38.596458331990505 23.04902680597704 3 18.618389173422926 25.96835158602417 31.061898213441346 24.351361027111558 4 13.76940673516838 18.893851252531313 37.04067211055276 30.29606990174754 5 14.157455569369434 36.686688328808344 34.52125534181148 14.634600760010732 6 29.59183733240705 36.55422633841518 18.78218379123084 15.071752537946928 7 25.388971031030984 33.25057935594833 23.56303322421837 17.79741638880232 8 24.339327228732742 33.85505351629563 21.95190240136548 19.853716853606148 9 25.519372997934163 16.772516120492075 22.039139014314422 35.66897186725934 10-11 23.7606247952054 26.494147742596137 29.12354206008948 20.621685402108987 12-13 24.909550945669647 25.23230609137368 28.42687911882573 21.43126384413094 14-15 22.60252544278442 26.92801849468237 26.75612481098432 23.71333125154889 16-17 21.508557058236395 29.197590307088483 27.721810134877998 21.572042499797124 18-19 21.36672863640598 27.743567148499455 29.94116878482868 20.94853543026588 20-21 22.38506482816479 27.01902926772969 29.589102330134743 21.006803573970778 22-23 22.292088031505518 27.004441997009376 29.14324964374686 21.560220327738243 24-25 21.813195100343385 27.34939706108463 29.440873913648314 21.396533924923673 26-27 21.50591045559463 27.56529529726248 29.734097592735182 21.194696654407704 28-29 21.525901839279694 27.344190483498853 29.800859172103007 21.32904850511845 30-31 21.87715435568621 27.099239323913764 29.570601951505804 21.453004368894224 32-33 21.696013729300255 27.41809790033664 29.248622457421742 21.63726591294136 34-35 21.986925745993453 27.50663583709379 29.11320846458065 21.393229952332106 36-37 21.89312853673518 27.233450371398384 29.424113551625386 21.44930754024105 38-39 21.676506689057735 27.512720177761796 29.42872029139626 21.382052841784212 40-41 21.78921545656473 27.413916718994553 29.334272111266273 21.462595713174444 42-43 21.911072726276988 27.40779659239904 28.997438310192607 21.683692371131364 44-45 21.941636208444958 27.638470916230567 28.842847292562375 21.5770455827621 46-47 21.946356494689546 27.6174129032636 28.697049226425154 21.7391813756217 48-49 21.83086007166678 27.838841144887354 28.71487261452506 21.615426168920806 50-51 21.819555831541702 28.02031036725034 28.407127081856583 21.75300671935138 52-53 22.033422773899286 28.028752184030846 28.127037232930295 21.810787809139573 54-55 22.032936397307736 27.952626548249633 28.16906522854472 21.845371825897907 56-57 21.87922966094174 28.15171523780708 28.196675407782884 21.772379693468295 58-59 21.78333722850233 28.016076211763647 28.40790678304143 21.792679776692598 60-61 21.883999270174108 28.026105040455256 28.45082153736126 21.63907415200938 62-63 21.835286964752633 27.999329915747452 28.39051502769369 21.77486809180623 64-65 21.88898103186002 27.923938792721376 28.320771977782965 21.86630819763564 66-67 21.85077776238405 27.97252795694547 28.206118821155513 21.97057545951497 68-69 21.736052242613912 28.043731571760073 28.330118063761343 21.890098121864675 70-71 21.752991431713642 27.969399604901103 28.32220622756701 21.95540273581825 72-73 21.841980709324215 27.958563590928954 28.20517401557922 21.99428168416761 74-75 21.75831390593616 28.244014160500708 27.938056448143712 22.059615485419425 76-77 21.81644404829658 28.131812961318857 28.06883677715423 21.98290621323033 78-79 21.70638411173316 28.131327821309434 28.059886508195248 22.102401558762164 80-81 21.762204554195417 28.2229885549031 27.984525847031783 22.0302810438697 82-83 21.817618323938035 28.1317337695283 27.982719367829763 22.067928538703907 84-85 21.830985915492956 28.061636047621253 27.946994030062335 22.160384006823456 86-87 21.737096490714162 28.229597515964617 27.904915343605367 22.12839064971586 88-89 21.801865129574956 28.151770232906888 27.872528000374903 22.17383663714326 90-91 21.778127845869534 28.104983715291926 27.96028282872072 22.156605610117815 92-93 21.84834659149833 28.026503049368184 28.016776944678973 22.108373414454515 94-95 21.76580231603871 27.982021381973 28.003314317111844 22.24886198487645 96-97 21.79295254293673 27.85024680859039 27.962728037315305 22.394072611157576 98-99 21.76184451746294 27.991395922288014 27.861163336906163 22.38559622334288 100-101 21.742999063045495 28.041776803256546 27.926195642429096 22.289028491268862 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5483.0 1 3206.5 2 889.0 3 772.0 4 637.5 5 628.5 6 830.0 7 1492.0 8 2364.0 9 2639.0 10 2118.0 11 1526.0 12 1485.0 13 1612.0 14 1724.5 15 1846.5 16 1933.5 17 2090.0 18 2297.5 19 2491.5 20 2656.5 21 2954.0 22 3407.0 23 4084.5 24 4901.5 25 6123.5 26 8387.5 27 11624.0 28 15350.0 29 20059.5 30 25870.5 31 31681.5 32 36811.0 33 41760.5 34 46899.0 35 50805.0 36 54187.5 37 56467.0 38 55611.0 39 53962.5 40 53735.5 41 53388.5 42 52814.0 43 53729.5 44 54860.0 45 55822.5 46 56772.5 47 57077.5 48 57521.5 49 57619.0 50 56273.0 51 54262.5 52 53136.5 53 52033.0 54 49562.0 55 46999.5 56 43826.5 57 39481.5 58 36171.0 59 32859.5 60 28120.0 61 23210.0 62 18379.0 63 13820.5 64 10111.0 65 7343.5 66 5369.5 67 4071.0 68 3033.5 69 2187.0 70 1658.0 71 1276.5 72 916.5 73 656.0 74 536.0 75 398.0 76 289.0 77 196.5 78 122.0 79 95.0 80 77.0 81 60.0 82 55.5 83 50.5 84 36.0 85 22.0 86 15.5 87 14.5 88 13.5 89 10.0 90 10.0 91 12.0 92 8.5 93 5.5 94 4.0 95 3.0 96 2.0 97 1.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1407785651469486 2 0.14452329838444017 3 0.13914024435554603 4 0.14294348904987342 5 0.1315922664237271 6 0.11000153885131478 7 0.11696440221477565 8 0.10707596600952449 9 0.10344825568570455 10-11 0.09148266276278226 12-13 0.11061590914809075 14-15 0.128608182125101 16-17 0.13905247717029234 18-19 0.13574657985906932 20-21 0.12696986133369845 22-23 0.12079690263752095 24-25 0.10558392386021144 26-27 0.11003079457973268 28-29 0.11459468821292554 30-31 0.10356527859937616 32-33 0.10353602287095824 34-35 0.11983146359973015 36-37 0.12126499429220738 38-39 0.10757331339262884 40-41 0.1101770732218222 42-43 0.1050573207486892 44-45 0.12193787604581915 46-47 0.12035806671125239 48-49 0.13334761012880128 50-51 0.12035806671125239 52-53 0.10461848482242064 54-55 0.0968364610632585 56-57 0.11705216940002938 58-59 0.10672489726850966 60-61 0.10593499260122627 62-63 0.10643233998433063 64-65 0.12746720871680278 66-67 0.1183394214504171 68-69 0.1233421510098785 70-71 0.111142512259613 72-73 0.11240050858158282 74-75 0.09604655639597512 76-77 0.12126499429220738 78-79 0.12120648283537158 80-81 0.1223767119720877 82-83 0.12784753318623554 84-85 0.11784207406731276 86-87 0.126794326963191 88-89 0.11392180645931377 90-91 0.12272778071310253 92-93 0.13574657985906932 94-95 0.11281008877943345 96-97 0.09777264437263139 98-99 0.10163440052379456 100-101 0.14475734421178338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1709067.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.426706151594914 #Duplication Level Percentage of deduplicated Percentage of total 1 60.83601720536962 29.460879286378066 2 19.5949485765794 18.978376335472475 3 8.486673915404186 12.329449917170521 4 4.128729599565452 7.99763100390193 5 2.2885191753665284 5.541272281388259 6 1.3348544838998488 3.8785563508174117 7 0.8526571609535771 2.890396444708643 8 0.5603886600306001 2.1710221575990314 9 0.3886770785188816 1.6940115602364838 >10 1.4731476776818444 11.282390676725084 >50 0.04099431174843056 1.3597565523408555 >100 0.013303894560526532 1.0212655953088206 >500 3.6275344036608783E-4 0.10546028208673881 >1k 4.836712538214504E-4 0.57537630859761 >5k 2.418356269107252E-4 0.7141552472681102 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6592 0.3857075234616314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5604 0.32789820410785536 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3250 0.19016223471636864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2934 0.17167261435625403 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2009 0.11754951678313373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.851145683580573E-5 0.0 5 0.0 0.0 0.0 5.851145683580573E-5 0.0 6 0.0 0.0 0.0 5.851145683580573E-5 0.0 7 0.0 0.0 0.0 5.851145683580573E-5 0.0 8 0.0 0.0 0.0 5.851145683580573E-5 0.0 9 0.0 0.0 0.0 1.1702291367161146E-4 0.0 10-11 0.0 0.0 0.0 1.1702291367161146E-4 0.0 12-13 0.0 0.0 0.0 1.1702291367161146E-4 8.77671852537086E-5 14-15 0.0 0.0 0.0 1.1702291367161146E-4 1.755343705074172E-4 16-17 0.0 0.0 0.0 1.4627864208951433E-4 2.9255728417902865E-4 18-19 0.0 0.0 0.0 1.755343705074172E-4 3.510687410148344E-4 20-21 0.0 0.0 0.0 2.0479009892532008E-4 4.3883592626854304E-4 22-23 0.0 0.0 0.0 4.3883592626854304E-4 4.973473831043488E-4 24-25 0.0 0.0 0.0 7.899046672833774E-4 5.266031115222517E-4 26-27 0.0 0.0 0.0 0.0012287405935519206 5.266031115222517E-4 28-29 0.0 0.0 0.0 0.002896317113372384 5.266031115222517E-4 30-31 0.0 0.0 0.0 0.007665000845490552 5.266031115222517E-4 32-33 0.0 0.0 0.0 0.017056089667637372 5.851145683580573E-4 34-35 0.0 0.0 0.0 0.03305897311223024 7.606489388654746E-4 36-37 0.0 0.0 0.0 0.051987429398613394 7.606489388654746E-4 38-39 0.0 0.0 0.0 0.07114993151233978 7.606489388654746E-4 40-41 0.0 0.0 0.0 0.08782569671054441 7.899046672833774E-4 42-43 0.0 0.0 0.0 0.1102063289502401 8.484161241191832E-4 44-45 0.0 0.0 0.0 0.1407785651469486 8.776718525370861E-4 46-47 0.0 0.0 0.0 0.174393397099119 0.0010239504946266003 48-49 0.0 0.0 0.0 0.20549223640734973 0.0013457635072235319 50-51 0.0 0.0 0.0 0.23626926270298354 0.0013750192356414346 52-53 0.0 0.0 0.0 0.27371659507789925 0.0014920421493130461 54-55 0.0 0.0 0.0 0.3185656267425443 0.0016090650629846577 56-57 0.0 0.0 0.0 0.3705238004127398 0.0016383207914025606 58-59 0.0 0.0 0.0 0.4196734241548167 0.0016675765198204636 60-61 0.0 0.0 0.0 0.4704321129598781 0.0018138551619099777 62-63 0.0 0.0 0.0 0.5291191041661912 0.0018138551619099777 64-65 0.0 0.0 0.0 0.6010881960742323 0.0018138551619099777 66-67 0.0 0.0 0.0 0.6937703437021486 0.0018138551619099777 68-69 0.0 0.0 0.0 0.7919233125442127 0.0018138551619099777 70-71 0.0 0.0 0.0 0.883727788319592 0.0018138551619099777 72-73 0.0 0.0 0.0 0.9852451659297149 0.0018723666187457834 74-75 0.0 0.0 0.0 1.1103426606446676 0.0018723666187457834 76-77 0.0 0.0 0.0 1.2410279994874398 0.0018723666187457834 78-79 0.0 0.0 0.0 1.3802267552998215 0.0019308780755815892 80-81 0.0 0.0 0.0 1.5217952251140534 0.0020771567176711037 82-83 0.0 0.0 0.0 1.6808001090653555 0.0023112025450143263 84-85 0.0 0.0 0.0 1.860752094563876 0.0023404582734322292 86-87 0.0 0.0 0.0 2.080345592068655 0.0023697140018501322 88-89 0.0 0.0 0.0 2.3288437492503222 0.0025159926439396467 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3270 0.0 63.482037 1 GTATCAA 6030 0.0 40.72775 1 TGGTATC 1255 0.0 34.06559 2 GTGGTAT 1315 0.0 33.59498 1 TCAACGC 7415 0.0 32.99235 4 ATCAACG 7510 0.0 32.575005 3 CAACGCA 7595 0.0 32.08347 5 TATCAAC 7715 0.0 32.07886 2 AACGCAG 7810 0.0 31.319136 6 ACGCAGA 8565 0.0 28.33656 7 CGCAGAG 8835 0.0 27.420845 8 GCAGAGT 9585 0.0 24.680525 9 GAGTACT 6075 0.0 23.148342 12-13 CAGAGTA 9095 0.0 23.06094 10-11 GTACTTT 6500 0.0 21.488928 14-15 GTACATG 5220 0.0 21.476261 1 TACATGG 5320 0.0 20.62612 2 AGAGTAC 8685 0.0 20.594162 10-11 CATGGGG 3055 0.0 19.90288 4 ACATGGG 5235 0.0 19.599918 3 >>END_MODULE