FastQCFastQC Report
Fri 10 Feb 2017
SRR2031683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709067
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76400.44702753022555586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70540.41273981651977365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.21748708505868994No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT31190.18249723387087807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27920.1633639874855696No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.14639566500318596No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.1100600503081506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.060.8876841
GTATCAA61250.039.7979771
GTGGTAT14500.033.0981031
ATCAACG76350.031.8563693
TCAACGC76700.031.7100724
TATCAAC77950.031.4462552
CAACGCA78150.031.0563935
AACGCAG80200.030.2625566
TGGTATC15950.028.5920892
ACGCAGA89950.026.929497
CGCAGAG91700.026.2083878
CGTATAC1109.4730836E-525.9115833
GCAGAGT99300.023.72429
GAGTACT65500.022.9142112-13
CAGAGTA96200.021.97070710-11
GTACATG51550.021.7536961
TACATGG52350.020.5989232
GTACTTT72000.020.4167814-15
ACATGGG49700.020.1679343
AGAGTAC90750.019.54803510-11
Produced by FastQC (version 0.11.5)