FastQCFastQC Report
Fri 10 Feb 2017
SRR2031682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849176
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.3528126089291266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.3453936521993085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.15603361376204697No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.10574957370439109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.10421867787125402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.060.2372061
GTATCAA30800.037.788271
GTGGTAT8050.034.8175131
TGGTATC8400.031.104632
ATCAACG37850.031.0007843
TATCAAC38000.030.75342
TCAACGC38250.030.3040034
CAACGCA39100.029.4004945
ATAGGCC1503.3309334E-828.503153
AACGCAG41000.028.1472476
ACGCAGA44450.025.7489057
CGCAGAG44750.025.5823238
GCAGAGT47400.022.849289
GCGTCAG2503.8198777E-1022.8025191
GAGTACT28400.021.1611612-13
CAGAGTA46450.021.01809510-11
GTACTTT30250.020.02405514-15
GTACATG24900.019.4599841
GTATAGG1507.71303E-419.00211
AGAGTAC42200.018.8568710-11
Produced by FastQC (version 0.11.5)