##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031682_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849176
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.753240788717534	33.0	31.0	34.0	30.0	34.0
2	32.059761462876956	34.0	31.0	34.0	30.0	34.0
3	32.15237123988431	34.0	31.0	34.0	30.0	34.0
4	35.5889544688027	37.0	35.0	37.0	35.0	37.0
5	35.583074651191275	37.0	35.0	37.0	35.0	37.0
6	35.613852723110405	37.0	37.0	37.0	35.0	37.0
7	35.59649000913827	37.0	37.0	37.0	35.0	37.0
8	35.586316617520986	37.0	37.0	37.0	35.0	37.0
9	37.330689986528114	39.0	38.0	39.0	35.0	39.0
10-11	37.25253893185865	39.0	38.0	39.0	35.0	39.0
12-13	37.19678135039144	39.0	38.0	39.0	35.0	39.0
14-15	38.547924694056356	41.0	38.5	41.0	34.5	41.0
16-17	38.45640361950879	41.0	38.0	41.0	34.0	41.0
18-19	38.46783293451534	41.0	38.5	41.0	34.0	41.0
20-21	38.475114110620176	41.0	39.0	41.0	34.0	41.0
22-23	38.369104873430246	40.0	38.5	41.0	34.0	41.0
24-25	38.29051986867269	40.0	38.0	41.0	34.0	41.0
26-27	38.164513010259355	40.0	38.0	41.0	34.0	41.0
28-29	38.04104096206204	40.0	38.0	41.0	34.0	41.0
30-31	37.8538512628713	40.0	38.0	41.0	33.0	41.0
32-33	37.61929741302156	40.0	38.0	41.0	33.0	41.0
34-35	37.437791459014385	40.0	37.5	41.0	32.5	41.0
36-37	37.255511224999296	40.0	37.0	41.0	32.0	41.0
38-39	37.0567497197283	40.0	37.0	41.0	31.0	41.0
40-41	36.93491573007245	40.0	37.0	41.0	31.0	41.0
42-43	36.87321532874222	40.0	37.0	41.0	31.0	41.0
44-45	36.697738160287145	40.0	37.0	41.0	30.5	41.0
46-47	36.56129883557708	40.0	36.5	41.0	30.0	41.0
48-49	36.54219207796734	40.0	37.0	41.0	30.0	41.0
50-51	35.96229874607855	39.5	36.0	40.5	29.0	41.0
52-53	35.95914039021358	39.5	36.0	40.5	29.0	41.0
54-55	36.15280754519675	40.0	36.0	41.0	29.0	41.0
56-57	35.99657668139467	40.0	35.5	41.0	28.5	41.0
58-59	35.776996759211286	39.0	35.0	41.0	28.0	41.0
60-61	35.5052827682365	39.0	35.0	41.0	28.0	41.0
62-63	35.19960408678531	39.0	35.0	41.0	27.5	41.0
64-65	34.90203620921929	38.0	35.0	40.5	26.5	41.0
66-67	34.590059069026914	37.0	35.0	40.0	26.0	41.0
68-69	34.23172522539497	37.0	34.5	40.0	26.0	41.0
70-71	33.857014329185	36.0	34.0	39.0	26.0	41.0
72-73	33.491379878847255	36.0	34.0	39.0	25.5	41.0
74-75	33.09508158497178	35.0	34.0	38.5	24.5	40.0
76-77	32.72389174917803	35.0	34.0	37.0	24.0	39.0
78-79	32.34815044231114	35.0	33.5	37.0	23.0	39.0
80-81	32.017824926752525	35.0	33.0	36.0	22.0	39.0
82-83	31.714893025709628	35.0	33.0	36.0	20.5	37.0
84-85	31.4130174427916	35.0	33.0	35.5	20.0	37.0
86-87	31.154242465637275	35.0	33.0	35.0	18.5	37.0
88-89	30.892802552121115	35.0	32.5	35.0	17.5	36.0
90-91	30.66303510697429	35.0	32.0	35.0	13.0	36.0
92-93	30.4484247081877	35.0	32.0	35.0	7.0	36.0
94-95	30.19376842963061	35.0	32.0	35.0	2.0	35.5
96-97	29.91422272885715	35.0	32.0	35.0	2.0	35.0
98-99	29.635620295439345	35.0	31.0	35.0	2.0	35.0
100-101	29.017325030382395	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.12775005772655845
1101	2	0.09459930489808244
1101	3	0.21330276549512206
1101	4	0.26278081710918855
1101	5	0.11565091443238629
1101	6	0.17756625445454688
1101	7	0.23675585257163334
1101	8	0.1339804378537579
1101	9	0.1752635345570397
1101	10-11	0.14915655443700615
1101	12-13	0.29363778241678773
1101	14-15	0.3451317961893068
1101	16-17	0.3350205418737602
1101	18-19	0.26134556365702366
1101	20-21	0.2870784776815043
1101	22-23	0.2211606564367088
1101	24-25	0.04610685748934884
1101	26-27	-0.0764201283439121
1101	28-29	0.12810231283532403
1101	30-31	0.30806173829717665
1101	32-33	0.1419031236318773
1101	34-35	0.1422799763517233
1101	36-37	0.03503172967283774
1101	38-39	0.25792919562479
1101	40-41	-0.07132784069294473
1101	42-43	-0.0013093509218791155
1101	44-45	-0.16563484320350597
1101	46-47	0.11073816955408233
1101	48-49	0.2721628253302768
1101	50-51	0.34103136331096096
1101	52-53	0.19568715223251587
1101	54-55	0.4382351544206813
1101	56-57	0.18309072680518312
1101	58-59	-0.055798655784208506
1101	60-61	-0.08055197220843979
1101	62-63	0.16512157169077568
1101	64-65	0.3297634017259341
1101	66-67	0.11829972829401214
1101	68-69	-0.10051608608095819
1101	70-71	-0.11388136669078364
1101	72-73	0.2982361764604704
1101	74-75	0.2865206650909542
1101	76-77	0.2074766232467553
1101	78-79	0.12606772841085956
1101	80-81	0.032840032718254974
1101	82-83	-0.0017226374246028797
1101	84-85	0.03951598451305571
1101	86-87	-0.1282775596361354
1101	88-89	-0.17346610239203386
1101	90-91	-0.7497807585491678
1101	92-93	-0.6291174820429859
1101	94-95	-0.5318156587139988
1101	96-97	-0.6738544923467167
1101	98-99	-0.6245440064440047
1101	100-101	-0.6635153366017121
1102	1	0.0826308110157683
1102	2	0.15465972986121557
1102	3	0.14293401120305305
1102	4	0.21543504160946725
1102	5	0.14293236183302582
1102	6	0.02823080017186186
1102	7	0.07091676119222257
1102	8	-0.042885012581024284
1102	9	0.13685078529486105
1102	10-11	0.3097162862252816
1102	12-13	0.16166115704604778
1102	14-15	0.5124833527488732
1102	16-17	0.357830873400971
1102	18-19	0.22810170787420958
1102	20-21	0.12126424117723644
1102	22-23	0.261670420720435
1102	24-25	0.11026649574965575
1102	26-27	0.036318823664082345
1102	28-29	0.33188006974728523
1102	30-31	0.01795655614777303
1102	32-33	0.11855242870797866
1102	34-35	0.4422451795265161
1102	36-37	0.09035592009431781
1102	38-39	0.15025971759558132
1102	40-41	0.3253283501041224
1102	42-43	0.3830390739234204
1102	44-45	-0.20998790762550357
1102	46-47	-0.007976999100051785
1102	48-49	0.14658402893220313
1102	50-51	0.029203020284228387
1102	52-53	0.03511453273119969
1102	54-55	0.2520316353385752
1102	56-57	0.22858343328015707
1102	58-59	-0.06192676333401437
1102	60-61	-0.24870532862918537
1102	62-63	-0.11169545776501622
1102	64-65	0.07810226757036531
1102	66-67	-0.02049910002876487
1102	68-69	0.04496225979949031
1102	70-71	0.1114271559538551
1102	72-73	0.2567036800920519
1102	74-75	-0.0422458150720999
1102	76-77	0.30988147049973946
1102	78-79	0.30031144979323443
1102	80-81	0.2323832553461358
1102	82-83	0.35408042359135194
1102	84-85	0.33276853029665787
1102	86-87	0.2831074460620755
1102	88-89	0.2662138772822047
1102	90-91	0.08192872294130993
1102	92-93	0.3130820455302761
1102	94-95	0.09295857617009773
1102	96-97	-0.24362857073715105
1102	98-99	-0.270300358705704
1102	100-101	-0.22123019779184006
1103	1	0.20867883423406042
1103	2	0.3832575937924574
1103	3	0.5139239797734945
1103	4	0.38398338126258835
1103	5	0.3595698808413985
1103	6	0.35725883718850326
1103	7	0.33892190258956845
1103	8	0.4058801785188706
1103	9	0.42837906743706355
1103	10-11	0.39410276812966316
1103	12-13	0.47608951531086774
1103	14-15	0.5677507000623905
1103	16-17	0.5393895867680669
1103	18-19	0.48208388412573555
1103	20-21	0.44546018475022464
1103	22-23	0.6214951425697706
1103	24-25	0.5178728757887754
1103	26-27	0.5862201494814272
1103	28-29	0.49732021721482056
1103	30-31	0.47199328397994833
1103	32-33	0.2305311357642026
1103	34-35	0.16384225727321677
1103	36-37	0.347430243948871
1103	38-39	0.37569769013082066
1103	40-41	0.5571618715156248
1103	42-43	0.5983029983926542
1103	44-45	0.5693759068492099
1103	46-47	0.6874834536780625
1103	48-49	0.6411698736883906
1103	50-51	0.7516067839867446
1103	52-53	0.615828587963783
1103	54-55	0.5466493909867225
1103	56-57	0.46463667990143165
1103	58-59	0.541132938557638
1103	60-61	0.6006832836392135
1103	62-63	0.6712454072519094
1103	64-65	0.6653741002360647
1103	66-67	0.6704136049630591
1103	68-69	0.548623098717222
1103	70-71	0.5761593807848016
1103	72-73	0.4081083832940138
1103	74-75	0.40693296971122095
1103	76-77	0.34043035566866564
1103	78-79	0.3471295416645077
1103	80-81	0.5091736970534981
1103	82-83	0.47113100146373554
1103	84-85	0.40530252252751353
1103	86-87	0.5507301915753864
1103	88-89	0.4842731245773564
1103	90-91	0.5770226461448082
1103	92-93	0.15689249126660698
1103	94-95	0.5862465924713938
1103	96-97	0.59416693927556
1103	98-99	0.6145360846025483
1103	100-101	0.9384659891239835
1104	1	0.1335208401225465
1104	2	0.2097527223780986
1104	3	0.29597530536192096
1104	4	0.2359901866403291
1104	5	0.19722222927401845
1104	6	0.21812044419491627
1104	7	0.20624420378526054
1104	8	0.1930134640832506
1104	9	0.3614985724511044
1104	10-11	0.31390016891004535
1104	12-13	0.32942496542005273
1104	14-15	0.4805491569667595
1104	16-17	0.5103274995818055
1104	18-19	0.3500318793578927
1104	20-21	0.44219163381347215
1104	22-23	0.4647014806720762
1104	24-25	0.4443882099620282
1104	26-27	0.4069957384148779
1104	28-29	0.32547848639399746
1104	30-31	0.2685281106024249
1104	32-33	0.49029179293820846
1104	34-35	0.4198088484377749
1104	36-37	0.3009837106504065
1104	38-39	0.23009520705087994
1104	40-41	0.09408036658987129
1104	42-43	0.05654704793200693
1104	44-45	0.2970173204932465
1104	46-47	0.153466752656243
1104	48-49	0.27169287579724255
1104	50-51	-0.08175399951462481
1104	52-53	0.17981408069146454
1104	54-55	0.2570082354308596
1104	56-57	0.22092078744270083
1104	58-59	0.13767150066689737
1104	60-61	-0.004406996284586739
1104	62-63	0.05964858852829735
1104	64-65	0.014670130771968104
1104	66-67	0.141556969549967
1104	68-69	0.22949282191765974
1104	70-71	0.16759560753563818
1104	72-73	-0.002278137711307693
1104	74-75	-0.09397713070545421
1104	76-77	-0.03333481125665827
1104	78-79	-0.05498032918952589
1104	80-81	0.010228972070223108
1104	82-83	0.08965799714755107
1104	84-85	0.046827382192066835
1104	86-87	0.1468884132120074
1104	88-89	0.14074162932991285
1104	90-91	0.16793335782296026
1104	92-93	-0.005861818975755995
1104	94-95	0.22928602223235472
1104	96-97	0.2701338360708512
1104	98-99	0.30150908382488595
1104	100-101	0.04591556854212797
1105	1	-0.058627153759850614
1105	2	0.0013670531841256661
1105	3	0.10140955742010505
1105	4	0.2735808930930972
1105	5	0.19765827105593559
1105	6	0.1365090420277255
1105	7	0.15185938064250593
1105	8	0.10846366095286442
1105	9	0.19323200123636752
1105	10-11	0.22559031892144077
1105	12-13	0.33148640175469524
1105	14-15	0.26553174783591516
1105	16-17	0.15336547426534963
1105	18-19	0.20919038379965826
1105	20-21	0.09230064425894824
1105	22-23	-0.09023002171964833
1105	24-25	0.02069714881855589
1105	26-27	0.1345379596247298
1105	28-29	-0.05062219370101673
1105	30-31	0.18532873537871097
1105	32-33	0.20404500630581168
1105	34-35	-0.006038016291654458
1105	36-37	0.10231461429818722
1105	38-39	0.2632083501311584
1105	40-41	0.30407841416398185
1105	42-43	0.2774445130323855
1105	44-45	0.21840940015222543
1105	46-47	0.41838491347105133
1105	48-49	0.22950908744021348
1105	50-51	0.1425829891938406
1105	52-53	0.33926219497346466
1105	54-55	0.35980508551708823
1105	56-57	0.320112157720267
1105	58-59	0.3447815849466167
1105	60-61	0.39582566780054407
1105	62-63	0.317982193031888
1105	64-65	0.36759388854100195
1105	66-67	0.11933673590966976
1105	68-69	0.24055494160135993
1105	70-71	0.1474091508632327
1105	72-73	-0.1248188946017521
1105	74-75	0.14829126277344074
1105	76-77	0.30965597980122794
1105	78-79	0.24165824423796067
1105	80-81	0.1315120412837132
1105	82-83	0.22716247145687518
1105	84-85	0.3219697465798177
1105	86-87	0.28543580835046356
1105	88-89	0.11439201493402607
1105	90-91	0.0757593315953713
1105	92-93	0.09923401259496956
1105	94-95	0.1062522778049022
1105	96-97	-0.00946331338735007
1105	98-99	0.1304700678475328
1105	100-101	0.08115197477687275
1106	1	-0.17474339372685677
1106	2	-0.05721611359357581
1106	3	-0.12947778752453587
1106	4	-0.1790126159960792
1106	5	-0.02093885466977241
1106	6	0.033339726333423414
1106	7	0.09144886067169011
1106	8	0.08233187579376278
1106	9	0.05086659404751259
1106	10-11	0.044341310323993355
1106	12-13	0.07509859679927189
1106	14-15	0.18551207840918238
1106	16-17	0.14691897827854916
1106	18-19	-0.14321118975447433
1106	20-21	0.1627140194691279
1106	22-23	-0.0388404718957176
1106	24-25	0.11686954182638942
1106	26-27	0.029089211171317686
1106	28-29	0.02635664580280661
1106	30-31	0.01304041965268965
1106	32-33	-0.04445123429568554
1106	34-35	0.03651932643224853
1106	36-37	0.003263188264746475
1106	38-39	0.31856509642727815
1106	40-41	0.11726010256219155
1106	42-43	0.178537435211247
1106	44-45	0.09004101082447136
1106	46-47	0.1810050714609659
1106	48-49	0.15154836284391138
1106	50-51	0.34687219321655505
1106	52-53	0.41553674210077673
1106	54-55	0.27854021447873833
1106	56-57	0.25070604750802516
1106	58-59	0.49422322702650234
1106	60-61	0.2211230885676514
1106	62-63	-0.06778761122559018
1106	64-65	-0.015593510335939698
1106	66-67	-0.03274124384985555
1106	68-69	0.0796162023481628
1106	70-71	-0.22180978254624506
1106	72-73	-0.22430653776027754
1106	74-75	-0.09482919660285205
1106	76-77	0.16875943464086163
1106	78-79	0.1698410430070325
1106	80-81	0.3133067178485476
1106	82-83	0.25011859594531316
1106	84-85	0.28881248634061407
1106	86-87	0.07494280368690553
1106	88-89	0.03920404500921393
1106	90-91	0.06848164370476084
1106	92-93	0.15229546369106473
1106	94-95	0.3009304288787895
1106	96-97	0.40592801302995696
1106	98-99	0.45058840165673786
1106	100-101	0.355933866214329
1107	1	0.17153163077621159
1107	2	0.2703483998551306
1107	3	0.35625616847507047
1107	4	0.3503147619887983
1107	5	0.28434058208178214
1107	6	0.37378658095305894
1107	7	0.43680311233603675
1107	8	0.3389290520903927
1107	9	0.29116626067008866
1107	10-11	0.48996314569726707
1107	12-13	0.3704699751775564
1107	14-15	0.4613437414639776
1107	16-17	0.4764980002583954
1107	18-19	0.4560677925456602
1107	20-21	0.4504394222860455
1107	22-23	0.442085286342909
1107	24-25	0.3482472079131185
1107	26-27	0.4740259779868623
1107	28-29	0.3654155583073404
1107	30-31	0.44121335537494843
1107	32-33	0.42441036489851314
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2312	64-65	-0.08822326470163233
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2312	82-83	0.2609691551035773
2312	84-85	0.2959410255855879
2312	86-87	0.3669309895205508
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2312	100-101	0.2373594980390905
2313	1	0.17057036236147738
2313	2	0.08977163880629035
2313	3	-0.05318800646812605
2313	4	0.08209534257959916
2313	5	0.06712328180348948
2313	6	0.042539612842148244
2313	7	-0.46182583550270806
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2313	10-11	-0.2443284773527452
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2313	32-33	-0.45161537105267513
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2313	36-37	-0.09032928348064928
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2313	48-49	-0.4428279080219326
2313	50-51	-0.6724934378422205
2313	52-53	-0.403059276246978
2313	54-55	-0.511224291124897
2313	56-57	-0.9477228076487307
2313	58-59	-1.1269523534340777
2313	60-61	-0.8203792521899089
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2313	68-69	-0.2803012800370439
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2313	76-77	-1.0372272301342846
2313	78-79	-0.7086641823204332
2313	80-81	-0.628572744959417
2313	82-83	-0.5183414693121655
2313	84-85	-0.4092877169979232
2313	86-87	-0.41430744457526103
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2313	90-91	-0.27613343171965
2313	92-93	-0.0732164292488342
2313	94-95	-0.08521536012201025
2313	96-97	-0.20413133344944612
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2314	1	0.20475169230282475
2314	2	-0.01458847708657629
2314	3	-0.06162557271785829
2314	4	-0.04116096439516781
2314	5	0.09203318216388823
2314	6	0.029826730360341003
2314	7	0.0912658187973463
2314	8	-0.01644033563368197
2314	9	-0.01661249693837874
2314	10-11	-0.2708990377371734
2314	12-13	-0.31108103768018935
2314	14-15	-0.7707373935854065
2314	16-17	-0.35296838056800794
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2314	20-21	-0.11664308114757915
2314	22-23	-0.696098082213652
2314	24-25	-0.21031076849998698
2314	26-27	-0.1875795675169769
2314	28-29	-0.023902428850121282
2314	30-31	-0.12870791185022767
2314	32-33	-0.12254675399380943
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2314	82-83	-0.4291251595107397
2314	84-85	-0.4730605952397404
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2314	90-91	-0.5560889118977279
2314	92-93	-0.5327457911313864
2314	94-95	-0.3826077705523723
2314	96-97	-0.3960417991409173
2314	98-99	-0.22588156539572424
2314	100-101	0.0182673685093917
2315	1	-0.29304201384035977
2315	2	-0.4716141777194096
2315	3	-0.4573808785855249
2315	4	-0.855380259242132
2315	5	-0.8257958246810091
2315	6	-0.7178485892832072
2315	7	-0.471791135871392
2315	8	-0.5676263531118337
2315	9	-0.64038020110128
2315	10-11	-0.9390078672175619
2315	12-13	-0.5022967594073364
2315	14-15	-0.5195911970207732
2315	16-17	-0.3067223088473128
2315	18-19	-0.25249301500726773
2315	20-21	-0.4577199677978925
2315	22-23	-0.7871588252503727
2315	24-25	-0.6617292540137854
2315	26-27	-0.4819153116299404
2315	28-29	-0.2132185853785984
2315	30-31	-0.3466951261082656
2315	32-33	-0.22018871016927477
2315	34-35	-0.3842668343720632
2315	36-37	-0.2665708796636963
2315	38-39	-0.7489600382484909
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2315	66-67	-0.17807584471984228
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2315	70-71	-0.2135491885324754
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2315	74-75	-0.14799371709948872
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2315	84-85	-0.31616619599741824
2315	86-87	-0.3201973455367302
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2315	90-91	-0.20047739181507396
2315	92-93	-0.33801076164238353
2315	94-95	-0.06344314874342416
2315	96-97	-0.17834516992743588
2315	98-99	-0.5533870542243449
2315	100-101	-0.6654846225362832
2316	1	-0.21015165488020315
2316	2	-0.41853182177041504
2316	3	-0.7134307189727132
2316	4	-0.3770238628342142
2316	5	-0.39702503861898464
2316	6	-0.9586153699336606
2316	7	-0.6083346574377586
2316	8	-0.5564534563967669
2316	9	-0.5291492472238772
2316	10-11	-1.0333889281545012
2316	12-13	-0.6366169757279678
2316	14-15	-1.0098732802608907
2316	16-17	-0.9275779684441474
2316	18-19	-0.628628858114439
2316	20-21	-0.6882978940031563
2316	22-23	-0.5415668197393728
2316	24-25	-0.5650338961011414
2316	26-27	-0.6144543093538104
2316	28-29	-0.6123096445755962
2316	30-31	-0.7493881262254476
2316	32-33	-0.9239902114884373
2316	34-35	-0.9920019303705558
2316	36-37	-0.6728751418051004
2316	38-39	-0.4027458268494044
2316	40-41	-0.6089675379013428
2316	42-43	-0.4955684736758812
2316	44-45	-0.4467405863566185
2316	46-47	-0.439297422494036
2316	48-49	-0.42751804738927746
2316	50-51	-0.6894854989749959
2316	52-53	-0.6182113212921863
2316	54-55	-0.5731151054708121
2316	56-57	-0.4734554916503342
2316	58-59	-0.5134169345451767
2316	60-61	-0.5112941454348743
2316	62-63	-0.4201438261372843
2316	64-65	-0.5156248676913364
2316	66-67	-0.43175546150706623
2316	68-69	-0.6064693776816128
2316	70-71	-0.46189447063347444
2316	72-73	-0.35785170745002404
2316	74-75	-0.12753433758319233
2316	76-77	-0.2016789411874953
2316	78-79	-0.29704965454314447
2316	80-81	-0.3347354217915637
2316	82-83	-0.18152759552966558
2316	84-85	-0.1773691865626823
2316	86-87	4.7082671017406597E-4
2316	88-89	-0.05891660415772648
2316	90-91	-0.13536149054420932
2316	92-93	0.05042006156201495
2316	94-95	-0.17739408955352687
2316	96-97	-0.22124754867318686
2316	98-99	-0.22902594932730125
2316	100-101	-0.4254803969691139
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7879.0
3	987.0
4	758.0
5	758.0
6	776.0
7	915.0
8	1122.0
9	1278.0
10	1495.0
11	1734.0
12	2153.0
13	2663.0
14	3001.0
15	3679.0
16	4570.0
17	6336.0
18	6081.0
19	3723.0
20	3232.0
21	3341.0
22	3574.0
23	3771.0
24	4291.0
25	4829.0
26	5769.0
27	6923.0
28	8065.0
29	9737.0
30	11992.0
31	14945.0
32	18858.0
33	24962.0
34	35537.0
35	56166.0
36	97651.0
37	185053.0
38	231994.0
39	68503.0
40	75.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.21312194621446	19.183699463276735	13.791464555154182	23.811714035354626
2	17.04446546384336	21.43841093344215	37.67393093948064	23.843192663233847
3	19.114998744001575	25.34675580914013	30.39563360697981	25.14261183987848
4	14.3071285613027	18.778062009744083	35.977634128593145	30.937175300360064
5	14.743552702799912	36.207341823203315	33.79788228884505	15.25122318515173
6	30.191057270020927	35.96804904648215	18.399151118578125	15.441742564918796
7	26.15761566134222	32.82474610924878	22.67265337501076	18.344984854398238
8	25.14984885426344	33.394011704952206	21.24576155286328	20.210377887921073
9	26.068293442047015	16.547318980172644	21.245735196682	36.138652381098346
10-11	24.453966504483848	26.15237719145726	28.352719631979582	21.04093667207931
12-13	25.608812887084735	24.789516687161843	27.679985191595524	21.921685234157895
14-15	23.270495505322504	26.38097978426963	25.958398732079957	24.390125978327905
16-17	22.10570821033613	28.697080735306084	26.98547856575923	22.211732488598557
18-19	22.092195675080916	27.272239463220853	29.093580786051948	21.54198407564628
20-21	23.044757709562788	26.41990798582347	28.86300642141738	21.672327883196356
22-23	22.894954517610756	26.540325713925235	28.54928600106948	22.01543376739453
24-25	22.53187031877982	26.754857033045155	28.7486014550762	21.964671193098823
26-27	22.247499012574202	26.882092517375746	28.999722931269268	21.87068553878078
28-29	22.194140735998264	26.869737669004763	29.155657112672984	21.780464482323993
30-31	22.484809472947067	26.669955884398732	28.894716738227782	21.950517904426412
32-33	22.31036031628326	26.807269752082874	28.696327751887175	22.186042179746696
34-35	22.555172407694215	26.868445209036995	28.66695397843275	21.909428404836035
36-37	22.46300295551865	26.832607276837116	28.782082234520672	21.922307533123565
38-39	22.241524649402557	27.030517746508764	28.808823442722485	21.9191341613662
40-41	22.512099507766674	26.905591416865622	28.692427859816664	21.889881215551036
42-43	22.514089874566075	27.01548132520568	28.30548368899177	22.164945111236474
44-45	22.606793640873864	27.008245167718563	28.22742159426228	22.15753959714529
46-47	22.433627979452396	27.159482100439874	28.154082252901997	22.25280766720573
48-49	22.42211113351754	27.300579854735595	28.113992105862113	22.163316905884752
50-51	22.555714083577023	27.5366121120649	27.754810866899348	22.152862937458732
52-53	22.69890299039748	27.53871365160956	27.503168418384487	22.25921493960847
54-55	22.560243502637377	27.508512748935647	27.616318307552934	22.314925440874045
56-57	22.494602634833903	27.546430310612323	27.677370212675644	22.28159684187813
58-59	22.436506570399835	27.598921478239753	27.675082940539824	22.28948901082059
60-61	22.502828032626564	27.58692037120765	27.7546865172255	22.155565078940278
62-63	22.46782016547945	27.616534740545294	27.674068205451057	22.241576888524193
64-65	22.48328894543915	27.546162132458505	27.559605267114495	22.410943654987843
66-67	22.382515259117593	27.585973108108185	27.624117217378384	22.407394415395835
68-69	22.41799642123323	27.63533133071755	27.62348071917293	22.323191528876286
70-71	22.455197132616487	27.51780324467082	27.598330503678554	22.428669119034144
72-73	22.422600340860924	27.62075891370182	27.446850278813862	22.509790466623397
74-75	22.375694189847586	27.761581815674102	27.437754261214607	22.4249697332637
76-77	22.46776523302774	27.752675176134183	27.346284231937034	22.433275358901042
78-79	22.331181786452095	27.85471044911281	27.34095689988586	22.473150864549236
80-81	22.216051326133428	27.921378853321734	27.390052908555575	22.472516911989267
82-83	22.314876997395668	27.828664857333724	27.382738775050548	22.473719370220063
84-85	22.395694849843856	27.79511390454315	27.23851713328786	22.570674112325133
86-87	22.390449804221458	27.826849342206504	27.21507456447308	22.567626289098957
88-89	22.297484510313684	27.74342830530157	27.266151424579522	22.692935759805223
90-91	22.237846015431593	27.747738824418633	27.35903064091158	22.655384519238194
92-93	22.232233359612568	27.712426933602373	27.37779418346366	22.677545523321395
94-95	22.231346737134388	27.651839166177854	27.392566697223643	22.724247399464122
96-97	22.342891734004183	27.588940634333266	27.340436756175613	22.727730875486944
98-99	22.19134156009891	27.753456390970793	27.253366499753906	22.801835549176392
100-101	22.208187084842994	27.685487903834545	27.340801441727052	22.765523569595413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1886.0
1	1088.0
2	279.0
3	258.5
4	219.0
5	220.5
6	315.5
7	546.0
8	879.0
9	1092.0
10	987.0
11	793.0
12	741.0
13	743.5
14	818.0
15	917.5
16	990.5
17	1051.5
18	1085.0
19	1200.5
20	1375.0
21	1514.5
22	1735.0
23	2114.0
24	2592.5
25	3326.0
26	4447.5
27	5767.5
28	7195.5
29	8934.5
30	10787.5
31	12634.5
32	14586.0
33	16053.0
34	17314.0
35	18527.0
36	20141.5
37	22054.0
38	22987.5
39	23512.0
40	24383.5
41	25575.5
42	26828.5
43	28367.5
44	29469.0
45	30163.5
46	30925.5
47	31655.0
48	32495.5
49	32717.0
50	32156.5
51	31202.0
52	30022.5
53	29150.5
54	28182.5
55	26290.5
56	23961.5
57	21453.0
58	19662.5
59	17709.5
60	14882.0
61	12184.5
62	9646.0
63	7158.5
64	4986.5
65	3647.0
66	2778.5
67	2076.5
68	1596.0
69	1191.5
70	908.5
71	748.0
72	563.5
73	399.0
74	311.5
75	250.5
76	176.0
77	117.5
78	89.0
79	72.5
80	55.0
81	40.5
82	30.5
83	25.0
84	22.5
85	13.5
86	11.5
87	13.0
88	7.5
89	4.0
90	3.5
91	4.0
92	7.5
93	5.5
94	1.5
95	0.5
96	1.0
97	1.5
98	2.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1475548060708263
2	0.15096988139090128
3	0.14661271632735734
4	0.15038107530123318
5	0.13954704325134012
6	0.11787897915155397
7	0.12659330927864187
8	0.11634808331841692
9	0.11234420190867382
10-11	0.10050919950634496
12-13	0.11882106889502295
14-15	0.13813390863613667
16-17	0.1480258509425608
18-19	0.14478741744938622
20-21	0.1359553261043647
22-23	0.12959622033594922
24-25	0.1145816650494126
26-27	0.11905659133089018
28-29	0.12353151761236776
30-31	0.11187315703693934
32-33	0.1117553958190057
34-35	0.12771204084901128
36-37	0.1297139815538828
38-39	0.11552375479288157
40-41	0.11787897915155397
42-43	0.11310964982524235
44-45	0.12994950398975005
46-47	0.1291251754642147
48-49	0.14190226761001254
50-51	0.1290662948552479
52-53	0.11316853043420916
54-55	0.10474860335195531
56-57	0.12682883171450912
58-59	0.1151704711390807
60-61	0.11440502322251217
62-63	0.11469942626734624
64-65	0.1365441321940328
66-67	0.12676995110554232
68-69	0.13171592225875436
70-71	0.11964539742055828
72-73	0.12123517386266217
74-75	0.10421867787125402
76-77	0.129655100944916
78-79	0.1300083845987169
80-81	0.13059719068838496
82-83	0.13725069950163452
84-85	0.12665218988760868
86-87	0.13560204245056384
88-89	0.1214118156895626
90-91	0.1307738325152854
92-93	0.14502293988525347
94-95	0.12170621873439663
96-97	0.10592621553129151
98-99	0.10934129085136651
100-101	0.15356062818544094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	849176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.219895324328206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.47264069867681	38.772829287829914
2	20.07304749772131	23.77447543310648
3	7.828969720447924	13.908923020267833
4	3.2047749490245	7.591457480770406
5	1.442136680941687	4.2701591644370405
6	0.7464362425552721	2.652232569024484
7	0.38938473107339416	1.6141526110540663
8	0.24535145756194077	1.1623750107591588
9	0.14674463617493938	0.7821181794317882
>10	0.4397356377996432	3.806928489292829
>50	0.006587048129088645	0.2440912571041331
>100	0.0029933571040526756	0.283980599468329
>500	5.986713947513367E-4	0.27901713519374016
>1k	5.986713947513367E-4	0.8572597622598014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2996	0.3528126089291266	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2933	0.3453936521993085	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1325	0.15603361376204697	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.10574957370439109	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	885	0.10421867787125402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.1776121793362035E-4	0.0
14-15	0.0	0.0	0.0	1.1776121793362035E-4	0.0
16-17	0.0	0.0	0.0	1.1776121793362035E-4	0.0
18-19	0.0	0.0	0.0	1.1776121793362035E-4	0.0
20-21	0.0	0.0	0.0	1.1776121793362035E-4	0.0
22-23	0.0	0.0	0.0	4.710448717344815E-4	0.0
24-25	0.0	0.0	0.0	0.0011187315703693934	0.0
26-27	0.0	0.0	0.0	0.002001940704871546	0.0
28-29	0.0	0.0	0.0	0.002944030448340509	0.0
30-31	0.0	0.0	0.0	0.005417016024946536	0.0
32-33	0.0	0.0	0.0	0.010716270831959453	0.0
34-35	0.0	0.0	0.0	0.019783884612848218	0.0
36-37	0.0	0.0	0.0	0.032560976758646026	0.0
38-39	0.0	0.0	0.0	0.04580911377617832	0.0
40-41	0.0	0.0	0.0	0.0561132203453701	0.0
42-43	0.0	0.0	0.0	0.07024456649740454	0.0
44-45	0.0	0.0	0.0	0.0886153164950493	0.0
46-47	0.0	0.0	0.0	0.10598509614025832	0.0
48-49	0.0	0.0	0.0	0.12529793588137206	0.0
50-51	0.0	0.0	0.0	0.14643607450045693	0.0
52-53	0.0	0.0	0.0	0.17222578122791976	0.0
54-55	0.0	0.0	0.0	0.20631765381970285	0.0
56-57	0.0	0.0	0.0	0.23911415301421612	0.0
58-59	0.0	0.0	0.0	0.2694376666321234	0.0
60-61	0.0	0.0	0.0	0.30458939018530906	0.0
62-63	0.0	0.0	0.0	0.34456932367377313	0.0
64-65	0.0	0.0	0.0	0.4023312010702139	0.0
66-67	0.0	0.0	0.0	0.47016166259997927	0.0
68-69	0.0	0.0	0.0	0.5420548861484544	0.0
70-71	0.0	0.0	0.0	0.6115340047292905	0.0
72-73	0.0	0.0	0.0	0.6846637210660688	0.0
74-75	0.0	0.0	0.0	0.7774595607977617	0.0
76-77	0.0	0.0	0.0	0.8808539101434802	0.0
78-79	0.0	0.0	0.0	0.9858380359313028	0.0
80-81	0.0	0.0	0.0	1.0891735046680546	0.0
82-83	0.0	0.0	0.0	1.2073468868644426	0.0
84-85	0.0	0.0	0.0	1.3459518403723139	0.0
86-87	0.0	0.0	0.0	1.5088156047745107	0.0
88-89	0.0	0.0	0.0	1.7176062441708195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1735	0.0	60.237206	1
GTATCAA	3080	0.0	37.78827	1
GTGGTAT	805	0.0	34.817513	1
TGGTATC	840	0.0	31.10463	2
ATCAACG	3785	0.0	31.000784	3
TATCAAC	3800	0.0	30.7534	2
TCAACGC	3825	0.0	30.304003	4
CAACGCA	3910	0.0	29.400494	5
ATAGGCC	150	3.3309334E-8	28.50315	3
AACGCAG	4100	0.0	28.147247	6
ACGCAGA	4445	0.0	25.748905	7
CGCAGAG	4475	0.0	25.582323	8
GCAGAGT	4740	0.0	22.84928	9
GCGTCAG	250	3.8198777E-10	22.802519	1
GAGTACT	2840	0.0	21.16116	12-13
CAGAGTA	4645	0.0	21.018095	10-11
GTACTTT	3025	0.0	20.024055	14-15
GTACATG	2490	0.0	19.459984	1
GTATAGG	150	7.71303E-4	19.0021	1
AGAGTAC	4220	0.0	18.85687	10-11
>>END_MODULE