Basic Statistics
Measure | Value |
---|---|
Filename | SRR2031682_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 849176 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3942 | 0.46421472109433143 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3730 | 0.4392493428924039 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT | 3421 | 0.40286112655091527 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1751 | 0.20619989260176924 | No Hit |
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT | 1307 | 0.1539139118392418 | RNA PCR Primer, Index 33 (95% over 22bp) |
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1153 | 0.13577868427746428 | No Hit |
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT | 923 | 0.10869360415273158 | RNA PCR Primer, Index 33 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 1940 | 0.0 | 59.763382 | 1 |
TGGTATC | 755 | 0.0 | 41.535408 | 2 |
GTGGTAT | 805 | 0.0 | 40.138405 | 1 |
GTATCAA | 3420 | 0.0 | 38.207947 | 1 |
TATCAAC | 4170 | 0.0 | 31.448109 | 2 |
TCAACGC | 4150 | 0.0 | 31.370684 | 4 |
ATCAACG | 4190 | 0.0 | 31.0712 | 3 |
CAACGCA | 4285 | 0.0 | 30.593298 | 5 |
AACGCAG | 4490 | 0.0 | 29.302284 | 6 |
ACGCAGA | 5070 | 0.0 | 25.762783 | 7 |
CGCAGAG | 5090 | 0.0 | 25.568237 | 8 |
GAGTACT | 3645 | 0.0 | 23.520563 | 12-13 |
GCAGAGT | 5565 | 0.0 | 23.129816 | 9 |
GTACTTT | 3875 | 0.0 | 22.00193 | 14-15 |
CAGAGTA | 5345 | 0.0 | 21.860266 | 10-11 |
ATAACGT | 110 | 0.002871842 | 21.59727 | 3 |
GTACATG | 2400 | 0.0 | 20.590607 | 1 |
TACATGG | 2520 | 0.0 | 19.043306 | 2 |
AGAGTAC | 5110 | 0.0 | 18.822277 | 10-11 |
AGTACTT | 3925 | 0.0 | 18.454329 | 12-13 |