FastQCFastQC Report
Fri 10 Feb 2017
SRR2031682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849176
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.46421472109433143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.4392493428924039No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT34210.40286112655091527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.20619989260176924No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT13070.1539139118392418RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.13577868427746428No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT9230.10869360415273158RNA PCR Primer, Index 33 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.059.7633821
TGGTATC7550.041.5354082
GTGGTAT8050.040.1384051
GTATCAA34200.038.2079471
TATCAAC41700.031.4481092
TCAACGC41500.031.3706844
ATCAACG41900.031.07123
CAACGCA42850.030.5932985
AACGCAG44900.029.3022846
ACGCAGA50700.025.7627837
CGCAGAG50900.025.5682378
GAGTACT36450.023.52056312-13
GCAGAGT55650.023.1298169
GTACTTT38750.022.0019314-15
CAGAGTA53450.021.86026610-11
ATAACGT1100.00287184221.597273
GTACATG24000.020.5906071
TACATGG25200.019.0433062
AGAGTAC51100.018.82227710-11
AGTACTT39250.018.45432912-13
Produced by FastQC (version 0.11.5)