##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849176
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75893336599245	34.0	31.0	34.0	31.0	34.0
2	32.92407580996166	34.0	31.0	34.0	31.0	34.0
3	32.96808082187909	34.0	31.0	34.0	31.0	34.0
4	36.36813452099447	37.0	37.0	37.0	35.0	37.0
5	36.33848813437968	37.0	37.0	37.0	35.0	37.0
6	36.3692497197283	37.0	37.0	37.0	35.0	37.0
7	36.3201821530519	37.0	37.0	37.0	35.0	37.0
8	36.31396318313283	37.0	37.0	37.0	35.0	37.0
9	38.16163080445043	39.0	39.0	39.0	37.0	39.0
10-11	38.12555583294865	39.0	39.0	39.0	37.0	39.0
12-13	38.07905192798666	39.0	39.0	39.0	36.0	39.0
14-15	39.54926363910426	41.0	39.0	41.0	37.0	41.0
16-17	39.5186127493005	41.0	39.0	41.0	37.0	41.0
18-19	39.54046334328808	41.0	39.5	41.0	37.0	41.0
20-21	39.544818741933355	41.0	39.5	41.0	37.0	41.0
22-23	39.455227184941634	41.0	39.0	41.0	36.0	41.0
24-25	39.38624619631266	41.0	39.0	41.0	36.0	41.0
26-27	39.24568935061754	41.0	39.0	41.0	36.0	41.0
28-29	39.14067460691305	40.5	39.0	41.0	35.5	41.0
30-31	38.952894335214374	40.0	38.5	41.0	35.0	41.0
32-33	38.71507849962788	40.0	38.0	41.0	35.0	41.0
34-35	38.55391520721264	40.0	38.0	41.0	35.0	41.0
36-37	38.44720764600036	40.0	38.0	41.0	34.5	41.0
38-39	38.48103514465788	40.0	38.0	41.0	35.0	41.0
40-41	38.3983084778656	40.0	38.0	41.0	35.0	41.0
42-43	38.27163921260139	40.0	38.0	41.0	34.0	41.0
44-45	38.04392140145271	40.0	38.0	41.0	34.0	41.0
46-47	37.76436863500617	40.0	38.0	41.0	33.0	41.0
48-49	37.45302799419673	40.0	37.5	41.0	33.0	41.0
50-51	37.24396532638699	40.0	37.0	41.0	32.5	41.0
52-53	37.28803922861692	40.0	37.0	41.0	33.0	41.0
54-55	37.19668007574401	40.0	37.0	41.0	33.0	41.0
56-57	36.99137811242899	40.0	36.5	41.0	32.0	41.0
58-59	36.77379718692003	40.0	36.0	41.0	32.0	41.0
60-61	36.489677640442025	39.0	35.5	41.0	31.0	41.0
62-63	36.18366275071364	39.0	35.0	41.0	31.0	41.0
64-65	35.822668681168565	38.5	35.0	41.0	30.5	41.0
66-67	35.423598288222934	37.5	35.0	40.0	30.0	41.0
68-69	34.987201122028885	37.0	35.0	40.0	29.0	41.0
70-71	34.62489931415867	37.0	35.0	39.0	29.0	41.0
72-73	34.232504215851606	36.0	34.5	39.0	28.5	41.0
74-75	33.85133117280752	36.0	34.0	39.0	28.0	40.5
76-77	32.94758507070384	35.0	33.5	37.0	26.5	39.0
78-79	33.12972399125741	35.0	34.0	37.0	27.0	39.0
80-81	32.91826429385663	35.0	34.0	36.5	27.0	39.0
82-83	32.65545893901853	35.0	34.0	36.0	27.0	37.5
84-85	32.36803265754096	35.0	34.0	36.0	26.0	37.0
86-87	32.130423492891936	35.0	34.0	35.5	26.0	37.0
88-89	31.903444044579686	35.0	34.0	35.0	26.0	36.0
90-91	31.70967738136735	35.0	33.5	35.0	25.0	36.0
92-93	31.533214551518178	35.0	33.0	35.0	25.0	36.0
94-95	31.359556793880188	35.0	33.0	35.0	24.0	36.0
96-97	31.191251872403363	35.0	33.0	35.0	23.5	35.0
98-99	30.972201875700677	35.0	33.0	35.0	20.0	35.0
100-101	30.27054992133551	34.5	32.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1871208372259474
1101	2	0.15150023183087313
1101	3	0.2280842839248578
1101	4	0.0905000124925266
1101	5	0.11658613415410457
1101	6	0.1084739928071059
1101	7	0.15172030371196144
1101	8	0.14890426118069655
1101	9	0.2270924505110159
1101	10-11	0.08748188188913986
1101	12-13	0.19385916235925293
1101	14-15	0.22087839628721184
1101	16-17	0.08694853736517416
1101	18-19	0.20169481933033495
1101	20-21	0.20873882362018037
1101	22-23	0.06907662576693241
1101	24-25	0.112189896038835
1101	26-27	0.1341662868237492
1101	28-29	0.0552863832513637
1101	30-31	0.17506045974398887
1101	32-33	0.2782981431524192
1101	34-35	-0.11691035196812294
1101	36-37	0.22832500983003712
1101	38-39	-0.014549193520458914
1101	40-41	-0.08433702478378535
1101	42-43	0.1590172023996601
1101	44-45	-0.0891401224186481
1101	46-47	0.1128012601492614
1101	48-49	0.11190182876432431
1101	50-51	0.21467094012735544
1101	52-53	0.10757942113632879
1101	54-55	0.22706020910711544
1101	56-57	-0.052855318696487075
1101	58-59	0.19199703028347415
1101	60-61	0.17440203304349922
1101	62-63	0.36825279607933936
1101	64-65	0.41172126260454434
1101	66-67	0.042413322617910865
1101	68-69	0.07034384212666822
1101	70-71	0.11794394254643237
1101	72-73	0.05709957588690884
1101	74-75	-0.10117305119880626
1101	76-77	-0.2575060437638399
1101	78-79	-0.26703611475639377
1101	80-81	-0.07834619035499912
1101	82-83	0.03537436073506939
1101	84-85	0.031086726684790733
1101	86-87	0.0914871192340172
1101	88-89	0.05810937842330688
1101	90-91	0.09813910221862088
1101	92-93	-0.1418811427557536
1101	94-95	-0.020616204310414332
1101	96-97	0.13583397487113658
1101	98-99	-0.34628235180770517
1101	100-101	-0.31200488403453264
1102	1	0.24012063654584637
1102	2	0.14910495830757498
1102	3	0.1610092608650504
1102	4	0.16235637708960837
1102	5	0.16830268547573723
1102	6	0.17069218915325735
1102	7	0.14916117212288782
1102	8	0.13436139756768029
1102	9	0.1497993121135508
1102	10-11	0.2625145154196602
1102	12-13	0.22583910171336186
1102	14-15	0.15227433813048208
1102	16-17	0.17252755469546344
1102	18-19	0.22312892721522104
1102	20-21	0.36568263135023216
1102	22-23	0.2879477357488298
1102	24-25	0.2954089890015865
1102	26-27	0.26203081118428173
1102	28-29	0.22433451517735392
1102	30-31	0.06750973278492722
1102	32-33	0.2025993989680046
1102	34-35	0.324041829093197
1102	36-37	0.2437479201333943
1102	38-39	0.22450131342693425
1102	40-41	-0.012372035176802854
1102	42-43	-0.026790479781972465
1102	44-45	-0.14715508321238957
1102	46-47	-0.23987653045485757
1102	48-49	-0.016080526115423766
1102	50-51	-0.10203632283538155
1102	52-53	-0.09091621453170262
1102	54-55	0.08634387319440151
1102	56-57	-0.04670880012805867
1102	58-59	-0.010520492403500725
1102	60-61	-0.003935378330467643
1102	62-63	-0.03478281267177863
1102	64-65	-0.09739311216647195
1102	66-67	-0.10492085394007233
1102	68-69	0.03263255267415133
1102	70-71	-0.12816719004420207
1102	72-73	-0.11319774365288993
1102	74-75	-0.1409831967747337
1102	76-77	-0.15573821775533503
1102	78-79	-0.18809334909000341
1102	80-81	-0.2863225800647129
1102	82-83	-0.056213092523954344
1102	84-85	0.1153834165677523
1102	86-87	0.14923327915580842
1102	88-89	0.0027051000708020467
1102	90-91	-0.06544003662439835
1102	92-93	0.14762476073736863
1102	94-95	0.14932670312082408
1102	96-97	-0.1501867227960041
1102	98-99	-0.23311902582036836
1102	100-101	-0.25577855370035607
1103	1	0.274067407751879
1103	2	0.22768954088993354
1103	3	0.1645337208311446
1103	4	0.19451150324459832
1103	5	0.10861505567061158
1103	6	0.15379902646296983
1103	7	0.1679832501815639
1103	8	0.1630009774135459
1103	9	0.15202950908754076
1103	10-11	0.1272408572370054
1103	12-13	0.08433658003981037
1103	14-15	0.19444930608588606
1103	16-17	0.229936853778959
1103	18-19	0.17018626163958572
1103	20-21	0.2178353686451473
1103	22-23	0.2633308892937052
1103	24-25	0.10333394314081801
1103	26-27	0.18402472493929878
1103	28-29	0.29213446419124267
1103	30-31	0.21854095375982752
1103	32-33	0.2482079075884016
1103	34-35	0.2896110463136736
1103	36-37	0.17784301459141716
1103	38-39	0.049399653169196256
1103	40-41	0.08670415341478588
1103	42-43	0.056572714302213
1103	44-45	0.13745530975509013
1103	46-47	0.015043362509942426
1103	48-49	0.02206725162075429
1103	50-51	-0.08657112799175337
1103	52-53	-0.07043352217944232
1103	54-55	0.16984433759592577
1103	56-57	-0.14312782757676956
1103	58-59	0.034538043581818556
1103	60-61	-0.04811324500711578
1103	62-63	0.05590384197927989
1103	64-65	0.3010723126495094
1103	66-67	0.3699907671510658
1103	68-69	0.26607409780310576
1103	70-71	0.34105484380273765
1103	72-73	0.38461484492657405
1103	74-75	0.5705601672750618
1103	76-77	0.43830621147647264
1103	78-79	0.5427257981582656
1103	80-81	0.5216121790971968
1103	82-83	0.4764590257353163
1103	84-85	0.3367558701587967
1103	86-87	0.40307194553010817
1103	88-89	0.4700463522969862
1103	90-91	0.5193591475977151
1103	92-93	0.5900496676342222
1103	94-95	0.3707749227961159
1103	96-97	0.57596077683408
1103	98-99	0.6544601497376696
1103	100-101	0.5862660965901405
1104	1	0.0721269921619978
1104	2	0.09576912713383479
1104	3	0.19906490026016144
1104	4	0.11991043625373266
1104	5	-0.033399662147324705
1104	6	0.017321318829331744
1104	7	0.08113318243341894
1104	8	0.031627303086864345
1104	9	0.0735995681421926
1104	10-11	0.18821503823153307
1104	12-13	0.15792906559875775
1104	14-15	0.2027064298289858
1104	16-17	0.17678342375312184
1104	18-19	0.20630926782351366
1104	20-21	0.2384289374952644
1104	22-23	0.09570856059124111
1104	24-25	0.14712573389923023
1104	26-27	0.13443019505032083
1104	28-29	0.13335674942621978
1104	30-31	0.29881062194672836
1104	32-33	0.2969076185975581
1104	34-35	0.14771858079051725
1104	36-37	0.15655073991767665
1104	38-39	0.1753831898601632
1104	40-41	0.2812916028583672
1104	42-43	0.4158042907115984
1104	44-45	0.5896020260924146
1104	46-47	0.6266121650826761
1104	48-49	0.6013665422178534
1104	50-51	0.6934460552474562
1104	52-53	0.7011949463049305
1104	54-55	0.6535245052173977
1104	56-57	0.597282159077352
1104	58-59	0.5822690749497212
1104	60-61	0.6824095873068856
1104	62-63	0.8905499725243544
1104	64-65	0.9114933020101574
1104	66-67	0.8059322626362189
1104	68-69	0.8675027517034621
1104	70-71	0.9767846051051308
1104	72-73	0.7747949293485732
1104	74-75	0.7804996184301771
1104	76-77	0.9028326706085466
1104	78-79	0.9126468095922533
1104	80-81	1.1067481677956508
1104	82-83	1.00452866200078
1104	84-85	0.9187591438035838
1104	86-87	0.9739655418869901
1104	88-89	0.9844500497497215
1104	90-91	0.9564221380926021
1104	92-93	1.097767692716321
1104	94-95	1.0117980386675214
1104	96-97	1.0554419177196586
1104	98-99	1.1929899979750296
1104	100-101	1.0895643616263264
1105	1	0.1566702872060901
1105	2	0.11033422426702089
1105	3	0.08361470339055188
1105	4	0.05410717570249801
1105	5	0.11885701677623928
1105	6	-0.06084405164347828
1105	7	0.06594656305884428
1105	8	0.05028924054415995
1105	9	0.03806867100249889
1105	10-11	0.14086057531135054
1105	12-13	0.10536813364079478
1105	14-15	0.13462371205214652
1105	16-17	0.10373666315307872
1105	18-19	0.12943412545912736
1105	20-21	0.08758939436292223
1105	22-23	0.2453359726507287
1105	24-25	0.15121281209388826
1105	26-27	0.10989633673136012
1105	28-29	0.2871037801118419
1105	30-31	0.22314272959047798
1105	32-33	0.2553095580072764
1105	34-35	0.013785418836782526
1105	36-37	0.19073283617227332
1105	38-39	0.30503565805188515
1105	40-41	0.3643315299678065
1105	42-43	0.05490975425004052
1105	44-45	0.1419709695046052
1105	46-47	-0.036890486962484204
1105	48-49	0.036650132078257514
1105	50-51	-0.0325228361000498
1105	52-53	0.005623699030188334
1105	54-55	-0.04555622759311717
1105	56-57	-0.0416036565485598
1105	58-59	-0.1864416731547891
1105	60-61	-0.02527158154541098
1105	62-63	-0.16038881892402657
1105	64-65	-0.13328883980103967
1105	66-67	0.20982572526353493
1105	68-69	-0.0014618117666458375
1105	70-71	-0.12177059973688387
1105	72-73	-0.2500757224362644
1105	74-75	-0.18573968390297324
1105	76-77	-0.07635609643450181
1105	78-79	0.19440464370112664
1105	80-81	-0.03298207202733039
1105	82-83	-0.15550843409111081
1105	84-85	-0.08878047417175594
1105	86-87	0.019655561736655613
1105	88-89	-0.1189650685347452
1105	90-91	-0.10606850653334376
1105	92-93	-0.10804240132553034
1105	94-95	-0.17047718830198733
1105	96-97	-0.13246605794009625
1105	98-99	-0.014212826534421907
1105	100-101	-0.06369293556543809
1106	1	-0.004336660543032167
1106	2	0.013284638276729765
1106	3	-0.06118082974609251
1106	4	-0.04797714903338601
1106	5	-0.07750413927845301
1106	6	-0.06026983128244723
1106	7	0.033101792905618765
1106	8	-0.09631143836831058
1106	9	-0.05687121034644349
1106	10-11	-0.12383597619399467
1106	12-13	0.003118839519416383
1106	14-15	-0.018440911532131565
1106	16-17	-0.03250425559949832
1106	18-19	0.06672831028829052
1106	20-21	0.007720418124861794
1106	22-23	-0.04003568433984839
1106	24-25	-0.04120176866180003
1106	26-27	0.03212913391418937
1106	28-29	0.08648990364853404
1106	30-31	-0.0614183566694777
1106	32-33	0.030486424223447273
1106	34-35	-0.04486119770456298
1106	36-37	0.008062552869077422
1106	38-39	-0.22744586220444774
1106	40-41	0.08893893455522317
1106	42-43	-0.08963991105245128
1106	44-45	-0.1516856403745237
1106	46-47	-0.08405428252735447
1106	48-49	-0.19923070926561337
1106	50-51	-0.19067137199674988
1106	52-53	-0.08493973487101414
1106	54-55	-0.1640899035551726
1106	56-57	-0.15067062777409035
1106	58-59	-0.2416271050890657
1106	60-61	-0.157540545093525
1106	62-63	-0.2436222188796009
1106	64-65	-0.2233811592071504
1106	66-67	-0.2739009966044321
1106	68-69	-0.2638297569460448
1106	70-71	-0.38048066604188335
1106	72-73	-0.41766082443176344
1106	74-75	-0.4066955545923534
1106	76-77	-0.3157536409286479
1106	78-79	-0.32964916169844827
1106	80-81	-0.23971567819464923
1106	82-83	-0.3777380587857593
1106	84-85	-0.3470225984698416
1106	86-87	-0.3100881012182022
1106	88-89	-0.2682607761582325
1106	90-91	-0.17696885867953327
1106	92-93	-0.3370484607779609
1106	94-95	-0.36016818795301475
1106	96-97	-0.21804868539480893
1106	98-99	-0.26897790378450637
1106	100-101	-0.3592102097635923
1107	1	0.15627461053045977
1107	2	0.07764208127530026
1107	3	0.1411060104938855
1107	4	0.20035681171421516
1107	5	0.15209557041526978
1107	6	0.20736135765064034
1107	7	0.154531890477017
1107	8	0.12206773012124472
1107	9	0.1967058988677266
1107	10-11	0.2635974246287702
1107	12-13	0.1935517892821892
1107	14-15	0.21512507379654977
1107	16-17	0.2664959512776406
1107	18-19	0.31321309464430414
1107	20-21	0.27686672774193255
1107	22-23	0.3055619023196243
1107	24-25	0.2817758377357862
1107	26-27	0.2052903861652453
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2309	5	0.04341316996910649
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2309	7	0.03528776902803088
2309	8	0.029374181431983004
2309	9	0.03293797715905811
2309	10-11	-0.004500096558800237
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2311	34-35	0.22984232940589067
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2312	56-57	9.831527844852417E-4
2312	58-59	-0.058857871590753064
2312	60-61	0.0022719031149662783
2312	62-63	-5.84771874834189E-4
2312	64-65	0.10169087375696506
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2312	68-69	0.14748226932793074
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2312	96-97	0.17868252584866795
2312	98-99	0.17629478248120378
2312	100-101	0.32221022707167535
2313	1	-0.2372633168063345
2313	2	-0.20196575184952792
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2313	80-81	0.1984067505186431
2313	82-83	-0.5248002898350848
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2313	86-87	-0.7696804783875422
2313	88-89	-0.29022133794005
2313	90-91	-0.08333146330430097
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2314	1	-0.1870124511431257
2314	2	-0.18038070485638258
2314	3	-0.1720056445921898
2314	4	-0.1568941043430101
2314	5	-0.13904844949218642
2314	6	-0.08268542081808761
2314	7	-0.024457836504993224
2314	8	-0.09372773191115868
2314	9	-0.06982494299266762
2314	10-11	-0.08050433247796462
2314	12-13	-0.4296731122701729
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2314	20-21	-0.28828293294945695
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2314	58-59	-0.14437202919708625
2314	60-61	-0.12663814865298662
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2314	78-79	-0.35477517898886646
2314	80-81	-0.09637277036327419
2314	82-83	1.2021048291188663E-4
2314	84-85	0.014629424846873462
2314	86-87	0.0464985902295183
2314	88-89	0.09482742186491677
2314	90-91	0.07325994366340183
2314	92-93	-0.36806349837194574
2314	94-95	-0.17993530236826416
2314	96-97	-0.14335000048027524
2314	98-99	-0.09439819608830291
2314	100-101	0.12231664107136098
2315	1	-0.7682363523011659
2315	2	-0.7288868824791805
2315	3	-0.4119427921232983
2315	4	-0.26481954237306127
2315	5	-0.33933688205493695
2315	6	-0.20998319515189223
2315	7	-0.2117560413351569
2315	8	-0.1737031936185076
2315	9	-0.28180135179201216
2315	10-11	-0.23101124194457867
2315	12-13	-0.17589550669094223
2315	14-15	-0.39099586212700643
2315	16-17	-0.4941972812671196
2315	18-19	-0.31201308342588163
2315	20-21	-0.29768389726130096
2315	22-23	-0.24970137154637229
2315	24-25	-0.2739423845693878
2315	26-27	-0.27573463470275783
2315	28-29	-0.29189859633705595
2315	30-31	-0.3359156631540827
2315	32-33	-0.23952409056928303
2315	34-35	-0.3936858455310315
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2315	80-81	-0.05192940215973607
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2315	84-85	0.005255366557669561
2315	86-87	-0.10048363027072327
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2315	92-93	-0.20235468611140917
2315	94-95	-0.14747049544588364
2315	96-97	-0.13317414271627115
2315	98-99	-0.05562123625361082
2315	100-101	0.02598769880092533
2316	1	-0.5146392938640574
2316	2	-0.3967058151506251
2316	3	-0.3832568724942931
2316	4	-0.20456881355149648
2316	5	-0.37375217363682367
2316	6	-0.3007157096484292
2316	7	-0.33531925972596355
2316	8	-0.5548244958033735
2316	9	-0.49914464183991214
2316	10-11	-0.4082413826666169
2316	12-13	-0.8159637371725452
2316	14-15	-0.5048074302385359
2316	16-17	-0.45797014460375607
2316	18-19	-0.631089566191605
2316	20-21	-0.6868595210650952
2316	22-23	-0.5636286101204746
2316	24-25	-0.3675586711125547
2316	26-27	-0.49527371054636404
2316	28-29	-0.4164960134147435
2316	30-31	-0.36033118554426125
2316	32-33	-0.28102741598411285
2316	34-35	-0.22940271832725756
2316	36-37	-0.6219740771675504
2316	38-39	-0.4270603990098536
2316	40-41	-0.11969200330843677
2316	42-43	-0.3408076821948569
2316	44-45	-0.26247466111915685
2316	46-47	-0.2636639879772602
2316	48-49	-0.04760744350156898
2316	50-51	0.11755459142896285
2316	52-53	0.16173812753290662
2316	54-55	0.14125176386639282
2316	56-57	0.07676485156373758
2316	58-59	-0.06456663649403538
2316	60-61	0.09536872065658031
2316	62-63	0.22359482744334258
2316	64-65	0.21480304086671254
2316	66-67	0.32086325415221495
2316	68-69	0.30961091145339736
2316	70-71	0.22551474417038975
2316	72-73	0.11469357872162789
2316	74-75	0.23794140852907475
2316	76-77	-0.19114299475808139
2316	78-79	-0.0418484504671639
2316	80-81	0.07269242733330117
2316	82-83	0.15658140530542397
2316	84-85	0.22947541208016276
2316	86-87	0.19845039051622848
2316	88-89	0.1555513026950628
2316	90-91	0.07144759487325203
2316	92-93	0.22758833974977577
2316	94-95	0.21907126842658187
2316	96-97	0.2797516541937384
2316	98-99	0.30565144066094163
2316	100-101	0.4447888982938544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	1.0
5	15.0
6	13.0
7	44.0
8	124.0
9	232.0
10	470.0
11	820.0
12	1299.0
13	1798.0
14	2263.0
15	2984.0
16	4057.0
17	6231.0
18	6685.0
19	3896.0
20	2694.0
21	2720.0
22	3240.0
23	3567.0
24	4101.0
25	4865.0
26	4968.0
27	4704.0
28	5756.0
29	7485.0
30	9540.0
31	12221.0
32	16083.0
33	21695.0
34	30816.0
35	49176.0
36	87736.0
37	186231.0
38	276287.0
39	84262.0
40	94.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.222141082826184	19.502272622874166	14.329125732168539	23.94646056213112
2	16.469141850452672	21.45644719115943	38.42984257680387	23.64456838158403
3	18.656297215722343	25.43793226490618	31.273810266839856	24.631960252531616
4	13.93668835949233	19.207750522689125	36.0498247887158	30.80573632910274
5	14.195526015808266	36.271750496952336	34.55502746191602	14.977696025323372
6	29.710095433691013	36.29494945688526	18.579776159476953	15.415178949946773
7	25.502958161794492	33.12422866402254	22.74911208041678	18.623701093766194
8	24.599140814153955	33.71798072484385	21.668652905875813	20.01422555512638
9	25.384843660207075	16.192874033180402	21.472109433144603	36.95017287346793
10-11	23.875747710573375	26.179217370260915	28.818777743823272	21.126257175342435
12-13	25.158403651541995	24.61958688162812	28.24268952648775	21.979319940342133
14-15	22.656813966496895	26.18989018753496	26.414932140018255	24.738363705949894
16-17	21.594827383167445	28.77453589648172	27.487152069564893	22.14348465078595
18-19	21.555404690084313	26.89070385415483	29.93330581641347	21.62058563934739
20-21	22.21989316702309	26.115316495049317	29.90133965161521	21.76345068631238
22-23	22.09106239460371	26.465479476574938	29.589625707744922	21.853832421076433
24-25	21.973065654234222	26.680746982957597	29.867659943286196	21.478527419521985
26-27	21.594081296791874	26.968784316496354	30.03853751740665	21.398596869305116
28-29	21.463956656481653	26.72232013364369	30.1838307384026	21.629892471472054
30-31	21.96683724389128	26.459665875899624	29.994035390799663	21.579461489409432
32-33	21.7476962934613	26.73006153030883	29.791326876085613	21.730915300144257
34-35	21.87862898761698	26.642039605872807	29.819472220062988	21.659859186447225
36-37	21.680336484192413	26.884406280049017	29.803558568823398	21.631698666935176
38-39	21.449322637474445	26.568638303484786	30.16070873411401	21.821330324926752
40-41	21.655699171903116	26.515527994196724	30.118432456875844	21.710340377024316
42-43	22.01522417025446	26.481023957342174	29.72175379426644	21.781998078136926
44-45	21.799191215955233	26.85438589880072	29.499479495416736	21.846943389827313
46-47	21.95522482971728	26.817938801850268	29.179227863246254	22.047608505186204
48-49	21.985136179072416	26.80840014319764	29.051986867268976	22.154476810460967
50-51	21.85919055649241	27.161860438825403	28.931046096451148	22.047902908231038
52-53	21.951397590134437	27.236815454040155	28.832362195822775	21.97942476000264
54-55	21.989493344135962	27.23646217038635	28.558037438646405	22.216007046831283
56-57	22.022113201503576	27.586389629476106	28.29542992265443	22.096067246365887
58-59	21.698039328829736	27.7133589307721	28.771505469730275	21.81709627066789
60-61	22.00485542366604	27.962671060061577	28.398045818571084	21.6344276977013
62-63	21.765366115636294	27.97417427886314	28.38974367430006	21.870715931200504
64-65	21.76945972920218	27.917965748403567	28.378422141946576	21.93415238044768
66-67	21.69382081851676	28.005137001425574	28.345248420890236	21.955793759167435
68-69	21.746319243098363	28.001427316386113	28.27122779828204	21.98102564223349
70-71	21.78812166146947	27.832981619829106	28.319570972330826	22.0593257463706
72-73	21.77676025195541	27.784991140812505	28.188173971006403	22.250074636225687
74-75	21.827635344411338	27.945073192561797	28.05506812855511	22.172223334471752
76-77	21.910308520178315	27.942105582356348	27.886109496783025	22.261476400682316
78-79	21.839954873741867	27.965738430213865	27.844973874581502	22.349332821462763
80-81	21.858716358058313	27.920787415021064	27.870675974479088	22.349820252441535
82-83	21.881521734009826	27.849364727164804	27.875098341271297	22.394015197554072
84-85	22.027035063619017	27.909166331530173	27.69136195742481	22.372436647426007
86-87	21.993747935458533	27.789832181115564	27.71416912841463	22.50225075501128
88-89	22.063021390248384	27.826694709670125	27.614546876251218	22.495737023830273
90-91	21.958227740774586	27.788821163104	27.743659736026455	22.509291360094963
92-93	22.048844997974506	27.649804045333358	27.811431316947257	22.489919639744883
94-95	22.05161238659595	27.616830904311946	27.701501220006218	22.63005548908589
96-97	22.1276272527721	27.506253120672277	27.68813532177075	22.677984304784875
98-99	22.0186981261835	27.602464035724044	27.53204282739974	22.84679501069272
100-101	21.89401130049955	27.91179816431652	27.50216094379662	22.692029591387314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1042.0
1	633.5
2	229.0
3	220.0
4	190.5
5	208.5
6	312.0
7	608.0
8	1018.5
9	1253.5
10	1081.5
11	840.5
12	834.5
13	844.0
14	895.0
15	988.5
16	1087.5
17	1206.5
18	1318.5
19	1398.5
20	1534.5
21	1758.0
22	2020.0
23	2395.0
24	2876.0
25	3598.0
26	4722.0
27	6183.5
28	7748.5
29	9407.0
30	11420.0
31	13478.0
32	15171.5
33	16599.5
34	18000.5
35	19451.5
36	21316.0
37	22920.5
38	23933.5
39	24978.0
40	26306.0
41	27550.0
42	28776.0
43	30259.5
44	31290.0
45	31769.5
46	32441.0
47	33247.5
48	33670.5
49	33507.0
50	32281.5
51	30746.0
52	29257.0
53	27711.5
54	26130.5
55	23822.5
56	21039.5
57	18503.5
58	16856.0
59	15144.5
60	12760.5
61	10442.5
62	8233.5
63	6198.0
64	4371.0
65	3078.5
66	2216.0
67	1618.0
68	1261.5
69	975.0
70	722.0
71	518.5
72	391.0
73	305.5
74	213.5
75	126.0
76	85.0
77	62.5
78	35.5
79	20.0
80	12.5
81	7.5
82	5.0
83	3.0
84	1.5
85	1.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018488511215578395
2	0.0
3	0.0017664182690043054
4	0.023670004804657693
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.8880608966810184E-5
12-13	5.8880608966810184E-5
14-15	1.1776121793362037E-4
16-17	0.0014131346152034443
18-19	1.7664182690043054E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.1216426276767125E-4
28-29	0.006182463941515069
30-31	5.8880608966810184E-5
32-33	1.1776121793362037E-4
34-35	0.01295373397269824
36-37	0.005004851762178865
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	2.944030448340509E-4
60-61	0.002649627403506458
62-63	0.011776121793362036
64-65	0.002178582531771977
66-67	0.005004851762178865
68-69	0.003532836538008611
70-71	0.0
72-73	0.006771270031183171
74-75	0.005417016024946536
76-77	0.0011187315703693934
78-79	3.532836538008611E-4
80-81	0.008066643428452994
82-83	0.010834032049893073
84-85	0.002296343749705597
86-87	0.001943060095904736
88-89	4.710448717344815E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0011776121793362036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	849176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16116379202518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.95497159217515	35.35624471110441
2	21.188554556128565	23.799477658859956
3	8.384239195807119	14.126058922417235
4	3.5119402643580786	7.889386096576892
5	1.6498813720208851	4.632962898573808
6	0.8415260913516174	2.835665079099656
7	0.4742963588492938	1.8645924846707438
8	0.2792032408684214	1.2544303153340541
9	0.18156102770171925	0.917701075350421
>10	0.5188930205486911	4.287417029793636
>50	0.007151631864492936	0.27913573831274296
>100	0.0056785001918832805	0.5958914338223928
>500	8.412592536144964E-4	0.3534008185288284
>1k	0.0012618888804217445	1.8076357375552106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3942	0.46421472109433143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3730	0.4392493428924039	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	3421	0.40286112655091527	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.20619989260176924	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	1307	0.1539139118392418	RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1153	0.13577868427746428	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	923	0.10869360415273158	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.1776121793362035E-4	0.0
14-15	0.0	0.0	0.0	1.1776121793362035E-4	0.0
16-17	0.0	0.0	0.0	1.1776121793362035E-4	0.0
18-19	0.0	0.0	0.0	1.1776121793362035E-4	0.0
20-21	0.0	0.0	0.0	1.1776121793362035E-4	0.0
22-23	0.0	0.0	0.0	4.710448717344815E-4	0.0
24-25	0.0	0.0	0.0	0.0011187315703693934	0.0
26-27	0.0	0.0	0.0	0.002001940704871546	0.0
28-29	0.0	0.0	0.0	0.002944030448340509	0.0
30-31	0.0	0.0	0.0	0.005534777242880156	0.0
32-33	0.0	0.0	0.0	0.010775151440926263	0.0
34-35	0.0	0.0	0.0	0.02007828765768227	0.0
36-37	0.0	0.0	0.0	0.032796499194513266	0.0
38-39	0.0	0.0	0.0	0.046162397429979185	0.0
40-41	0.0	0.0	0.0	0.05658426521710458	0.0
42-43	0.0	0.0	0.0	0.07106889502293989	0.0
44-45	0.0	0.0	0.0	0.0896751674564519	0.0
46-47	0.0	0.0	0.0	0.10710382771062771	0.0
48-49	0.0	0.0	0.0	0.12659330927864187	0.0
50-51	0.0	0.0	0.0	0.14737816424392586	0.0
52-53	0.0	0.0	0.0	0.17334451279828916	0.0
54-55	0.0	0.0	0.0	0.20737750478110545	0.0
56-57	0.0	0.0	0.0	0.24076281006528683	0.0
58-59	0.0	0.0	0.0	0.2713218461190613	0.0
60-61	0.0	0.0	0.0	0.3066502114991474	0.0
62-63	0.0	0.0	0.0	0.34733671229521323	0.0
64-65	0.0	0.0	0.0	0.40533411212752124	0.0
66-67	0.0	0.0	0.0	0.4739300215738551	0.0
68-69	0.0	0.0	0.0	0.5466475736478658	0.0
70-71	0.0	0.0	0.0	0.616362214664569	0.0
72-73	0.0	0.0	0.0	0.6903162595268826	0.0
74-75	0.0	0.0	0.0	0.7848785175275796	0.0
76-77	0.0	0.0	0.0	0.8875662995656967	0.0
78-79	0.0	0.0	0.0	0.9934925150969881	0.0
80-81	0.0	0.0	0.0	1.0984766408848108	0.0
82-83	0.0	0.0	0.0	1.2177098740426011	0.0
84-85	0.0	0.0	0.0	1.358905574345012	0.0
86-87	0.0	0.0	0.0	1.5241834437148483	0.0
88-89	0.0	0.0	0.0	1.7368013226939998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1940	0.0	59.763382	1
TGGTATC	755	0.0	41.535408	2
GTGGTAT	805	0.0	40.138405	1
GTATCAA	3420	0.0	38.207947	1
TATCAAC	4170	0.0	31.448109	2
TCAACGC	4150	0.0	31.370684	4
ATCAACG	4190	0.0	31.0712	3
CAACGCA	4285	0.0	30.593298	5
AACGCAG	4490	0.0	29.302284	6
ACGCAGA	5070	0.0	25.762783	7
CGCAGAG	5090	0.0	25.568237	8
GAGTACT	3645	0.0	23.520563	12-13
GCAGAGT	5565	0.0	23.129816	9
GTACTTT	3875	0.0	22.00193	14-15
CAGAGTA	5345	0.0	21.860266	10-11
ATAACGT	110	0.002871842	21.59727	3
GTACATG	2400	0.0	20.590607	1
TACATGG	2520	0.0	19.043306	2
AGAGTAC	5110	0.0	18.822277	10-11
AGTACTT	3925	0.0	18.454329	12-13
>>END_MODULE