FastQCFastQC Report
Fri 10 Feb 2017
SRR2031681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018143
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.17197977101448422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16440.16147044177487838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.052.6545521
GTATCAA26450.037.538421
TCAACGC30250.032.193084
CAACGCA30850.031.566965
ATCAACG31450.030.9662513
TATCAAC31650.030.9206712
AACGCAG31750.030.5180266
GTGGTAT8400.029.9745041
TGGTATC9250.027.220092
ACGCAGA36900.026.0025757
CGCAGAG37450.025.8756398
GCAGAGT40950.022.5040449
CAGAGTA39300.020.85066610-11
GAGTACT22000.020.29734612-13
GTACATG25350.019.1151471
AGAGTAC35050.018.63537210-11
TACATGG25450.018.2933712
CTATAAC5150.017.5267663
CGGAGAT1901.8633816E-417.5024911
ACATGGG24250.017.0436633
Produced by FastQC (version 0.11.5)