##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031681_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1018143 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.974590013387118 33.0 31.0 34.0 30.0 34.0 2 32.27924269969935 34.0 31.0 34.0 31.0 34.0 3 32.385792565484415 34.0 31.0 34.0 31.0 34.0 4 35.79839865323437 37.0 37.0 37.0 35.0 37.0 5 35.79485592888229 37.0 37.0 37.0 35.0 37.0 6 35.824163206936554 37.0 37.0 37.0 35.0 37.0 7 35.80848269840288 37.0 37.0 37.0 35.0 37.0 8 35.80183530211375 37.0 37.0 37.0 35.0 37.0 9 37.57864759665391 39.0 39.0 39.0 35.0 39.0 10-11 37.517826572495224 39.0 38.0 39.0 35.0 39.0 12-13 37.472538238734636 39.0 38.0 39.0 35.0 39.0 14-15 38.88645209955773 41.0 39.0 41.0 36.0 41.0 16-17 38.821076214244954 41.0 39.0 41.0 36.0 41.0 18-19 38.826559235785155 41.0 39.0 41.0 36.0 41.0 20-21 38.830625953328756 41.0 39.0 41.0 36.0 41.0 22-23 38.7400502679879 41.0 39.0 41.0 35.0 41.0 24-25 38.67703849066388 41.0 39.0 41.0 35.0 41.0 26-27 38.56924125589431 41.0 39.0 41.0 35.0 41.0 28-29 38.46493567210107 40.0 38.5 41.0 35.0 41.0 30-31 38.31488847833752 40.0 38.0 41.0 34.0 41.0 32-33 38.13950643475425 40.0 38.0 41.0 34.0 41.0 34-35 37.98993019644588 40.0 38.0 41.0 34.0 41.0 36-37 37.83170782493225 40.0 38.0 41.0 33.5 41.0 38-39 37.65806374939473 40.0 38.0 41.0 33.0 41.0 40-41 37.580406190486016 40.0 38.0 41.0 33.0 41.0 42-43 37.56025037740278 40.0 38.0 41.0 33.0 41.0 44-45 37.43655557225262 40.0 38.0 41.0 33.0 41.0 46-47 37.372627420706124 40.0 38.0 41.0 32.5 41.0 48-49 37.41613211503689 40.0 38.0 41.0 33.0 41.0 50-51 36.88873468658136 39.5 37.5 40.5 32.0 41.0 52-53 36.92216417536633 39.5 37.0 40.5 32.0 41.0 54-55 37.14598440494115 40.0 37.0 41.0 32.0 41.0 56-57 37.02201950020773 40.0 37.0 41.0 32.0 41.0 58-59 36.85103467783995 40.0 36.5 41.0 31.5 41.0 60-61 36.6389917722756 40.0 36.0 41.0 31.0 41.0 62-63 36.37754274203132 39.0 35.5 41.0 31.0 41.0 64-65 36.130235634876435 39.0 35.0 41.0 31.0 41.0 66-67 35.862129386540005 39.0 35.0 41.0 30.0 41.0 68-69 35.55317475050165 38.0 35.0 40.5 30.0 41.0 70-71 35.21792321903701 37.0 35.0 40.0 29.5 41.0 72-73 34.870866862513424 37.0 35.0 39.5 29.0 41.0 74-75 34.483418341038536 36.0 35.0 39.0 29.0 41.0 76-77 34.10790281915212 36.0 34.5 39.0 29.0 40.5 78-79 33.719462786661595 35.0 34.0 37.5 28.0 39.5 80-81 33.357299023810995 35.0 34.0 37.0 27.5 39.0 82-83 33.01111386121596 35.0 34.0 37.0 27.0 39.0 84-85 32.673673049856454 35.0 34.0 36.0 26.5 37.5 86-87 32.38564867607006 35.0 34.0 36.0 26.0 37.0 88-89 32.10692653193118 35.0 34.0 35.5 26.0 37.0 90-91 31.85727790693449 35.0 34.0 35.0 25.0 36.0 92-93 31.64501793952323 35.0 33.0 35.0 25.0 36.0 94-95 31.40080273596145 35.0 33.0 35.0 24.0 36.0 96-97 31.140703221453176 35.0 33.0 35.0 22.0 36.0 98-99 30.905623276887432 35.0 33.0 35.0 19.5 35.0 100-101 30.33166068027772 34.5 32.0 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05337380137206793 1101 2 0.210298658715125 1101 3 0.2601347258268163 1101 4 0.40423603007475606 1101 5 0.06592147770430756 1101 6 0.4177746096811603 1101 7 0.399931179197921 1101 8 0.3650499146399824 1101 9 0.3963621965500792 1101 10-11 0.23393104196600945 1101 12-13 0.3188730201046255 1101 14-15 0.3403115909280743 1101 16-17 0.4291981726115779 1101 18-19 0.43850362919178565 1101 20-21 0.41860315763671707 1101 22-23 0.40165895494933324 1101 24-25 0.37921074791664466 1101 26-27 0.20935634799282354 1101 28-29 0.18333819783041605 1101 30-31 0.20405981464866585 1101 32-33 0.21605530699125808 1101 34-35 0.18919691738427247 1101 36-37 -0.052126089177214396 1101 38-39 0.1424419389812357 1101 40-41 -0.02638395672733651 1101 42-43 0.005126512873118827 1101 44-45 -0.27748558071869667 1101 46-47 -0.14039345048913532 1101 48-49 0.08887170703921754 1101 50-51 -0.011225459702195906 1101 52-53 -0.0061477953922164374 1101 54-55 0.0495520368529867 1101 56-57 0.10540067956267762 1101 58-59 -0.10569300620086608 1101 60-61 -0.21889692375529535 1101 62-63 -0.03945662898034641 1101 64-65 0.06234228926506802 1101 66-67 -0.08248878749686384 1101 68-69 -0.10416529602425584 1101 70-71 -0.14858873089625035 1101 72-73 0.21894325893688205 1101 74-75 0.33441953782305944 1101 76-77 0.12703236238927929 1101 78-79 0.016799913352684825 1101 80-81 -0.051117218398346154 1101 82-83 0.018998862780684078 1101 84-85 0.09930170371968217 1101 86-87 0.03525179190020822 1101 88-89 -0.42661592722565445 1101 90-91 -0.639000034146175 1101 92-93 -0.6579604684095806 1101 94-95 -0.5941960975036942 1101 96-97 -0.5072744143993368 1101 98-99 -0.4875969145997203 1101 100-101 -0.6123518700108761 1102 1 -0.19370594073855685 1102 2 -0.05169523337852411 1102 3 0.03429013811049941 1102 4 0.0036808892535873383 1102 5 -0.035055787310959374 1102 6 0.05556219027158704 1102 7 0.08719687312394342 1102 8 0.03792809584120249 1102 9 0.1980208324509931 1102 10-11 0.18318112679803988 1102 12-13 0.2247515406339744 1102 14-15 0.3571130839718464 1102 16-17 0.26238107047381476 1102 18-19 0.1804154038779089 1102 20-21 0.17055361572969474 1102 22-23 0.21146689522758777 1102 24-25 0.027459136108028304 1102 26-27 -0.009673173554517689 1102 28-29 -0.00573340040508441 1102 30-31 -0.18302060611821958 1102 32-33 0.004982351443238997 1102 34-35 -0.011706374098586991 1102 36-37 -0.008701589686204159 1102 38-39 -0.12273824425595592 1102 40-41 -0.21713862248912363 1102 42-43 -0.16423826486695248 1102 44-45 -0.4116552447838444 1102 46-47 -0.3262027480799077 1102 48-49 -0.14679100079926144 1102 50-51 -0.16205070571509594 1102 52-53 -0.27948203360056567 1102 54-55 -0.1430363581249594 1102 56-57 -0.2838052926124135 1102 58-59 -0.4281850319898055 1102 60-61 -0.4728025905350748 1102 62-63 -0.3997728827977909 1102 64-65 -0.25895190822389935 1102 66-67 -0.7253723979481705 1102 68-69 -0.5964571182163212 1102 70-71 -0.6213481472518652 1102 72-73 -0.45345714825687367 1102 74-75 -0.767118975919729 1102 76-77 -0.5849058798910036 1102 78-79 -0.6979839346283114 1102 80-81 -0.7207344562673654 1102 82-83 -0.5763163730523715 1102 84-85 -0.345218146975931 1102 86-87 -0.4818584897421445 1102 88-89 -0.38550225230200397 1102 90-91 -0.17971194456354667 1102 92-93 -0.29462147960740737 1102 94-95 -0.6268048521694567 1102 96-97 -0.5839992192856585 1102 98-99 -0.3009202943078968 1102 100-101 -0.3784299154808437 1103 1 0.013031585671328116 1103 2 0.22129420266892907 1103 3 0.2358399512097975 1103 4 0.30367503844910004 1103 5 0.2628346726272781 1103 6 0.2641693737593158 1103 7 0.13193472457852096 1103 8 0.29339242218607353 1103 9 0.14992851975799937 1103 10-11 0.20657459339969364 1103 12-13 0.3806793807119817 1103 14-15 0.5486560711325765 1103 16-17 0.4285016620551545 1103 18-19 0.37356736227219045 1103 20-21 0.2573971067097034 1103 22-23 0.38500890725423886 1103 24-25 0.4530219509950726 1103 26-27 0.36193742290249986 1103 28-29 0.1854344851791936 1103 30-31 0.41584563799034413 1103 32-33 0.21700380439302336 1103 34-35 0.20449515361146808 1103 36-37 0.21092655918426573 1103 38-39 0.20800513295841228 1103 40-41 0.2595062661830667 1103 42-43 0.2575936870264286 1103 44-45 0.026139652014464332 1103 46-47 0.1711838925831799 1103 48-49 0.14130456234067168 1103 50-51 -0.022790507437825624 1103 52-53 -0.0698633110262179 1103 54-55 -0.11311729425367645 1103 56-57 -0.06387030155114104 1103 58-59 -0.029872664039210406 1103 60-61 -0.11651460093650456 1103 62-63 -0.0841954408995278 1103 64-65 -0.04928247867733404 1103 66-67 -0.05438718748172988 1103 68-69 -0.05486504930684788 1103 70-71 -0.09524250772643228 1103 72-73 -0.03859675495279191 1103 74-75 -0.02135008269691241 1103 76-77 -0.0538205556589233 1103 78-79 -0.1470442304189845 1103 80-81 -0.08494898576517329 1103 82-83 -0.08297068802067287 1103 84-85 -0.22454530446008647 1103 86-87 -0.1010237749688585 1103 88-89 0.12023673680899805 1103 90-91 0.09075319439306284 1103 92-93 -0.3531685298268137 1103 94-95 0.02654343321978203 1103 96-97 -0.1093865100381386 1103 98-99 0.028404587863402497 1103 100-101 0.15569457663681874 1104 1 -0.07671209837402415 1104 2 0.11553506218797338 1104 3 0.19016329081985361 1104 4 0.11705034738166376 1104 5 -0.01651514691687339 1104 6 0.10712007204498519 1104 7 0.041184557853007675 1104 8 0.003800017023472435 1104 9 -0.0017303990761021737 1104 10-11 0.15040403320197981 1104 12-13 0.128903018466481 1104 14-15 0.23262847806051212 1104 16-17 0.23235951874966787 1104 18-19 0.12771343343455754 1104 20-21 0.08038058617304245 1104 22-23 0.2907507622226575 1104 24-25 0.3412239144331508 1104 26-27 0.27944936339129356 1104 28-29 0.19144824984122266 1104 30-31 0.1462848126009959 1104 32-33 0.2529466371575282 1104 34-35 0.3994414091526437 1104 36-37 0.23895912459951063 1104 38-39 0.14001545032532903 1104 40-41 0.42589893908722587 1104 42-43 0.3862426464630886 1104 44-45 0.29862246457575736 1104 46-47 0.42709774453093274 1104 48-49 0.54259693023522 1104 50-51 0.44037786571347937 1104 52-53 0.43509432107923374 1104 54-55 0.48717361724450114 1104 56-57 0.39012019999842806 1104 58-59 0.35095782515305274 1104 60-61 0.5542417444402901 1104 62-63 0.43112859503472123 1104 64-65 0.37678885532289286 1104 66-67 0.4073560503276852 1104 68-69 0.515382783261515 1104 70-71 0.41978424191069763 1104 72-73 0.29077807341799655 1104 74-75 0.3117159994497314 1104 76-77 0.37324929252279304 1104 78-79 0.30474451364754884 1104 80-81 0.3067002727966752 1104 82-83 0.23456601611018613 1104 84-85 0.3412812372605174 1104 86-87 0.30840197823815174 1104 88-89 0.47801129449602797 1104 90-91 0.2690109618403902 1104 92-93 0.27554785536796444 1104 94-95 0.28161017246108244 1104 96-97 0.2730272585468505 1104 98-99 0.3440846318004773 1104 100-101 0.19154791703147112 1105 1 -0.21186601322420984 1105 2 0.034456382447508815 1105 3 0.03786368084683289 1105 4 0.08437191915391651 1105 5 -0.011787892415156875 1105 6 0.026483362122995402 1105 7 0.04571816578476984 1105 8 -0.0013760418815422781 1105 9 0.020327084338639168 1105 10-11 6.924526814628962E-4 1105 12-13 0.08027494745970642 1105 14-15 0.10856823347350542 1105 16-17 0.07525633474442373 1105 18-19 0.007165026348459946 1105 20-21 0.031102090510721325 1105 22-23 -0.19310936572076542 1105 24-25 0.04013083650065852 1105 26-27 -0.003635722633347882 1105 28-29 -0.14299646292395352 1105 30-31 -0.04357995374733292 1105 32-33 -0.002140572144796238 1105 34-35 -0.056917488565510155 1105 36-37 0.009605266248563282 1105 38-39 -0.035961439665193495 1105 40-41 -0.09255330083403379 1105 42-43 -0.17411926758516927 1105 44-45 -0.12905965432778999 1105 46-47 0.017587647570955767 1105 48-49 0.1466170330273755 1105 50-51 0.06432078326104573 1105 52-53 0.1076994825576918 1105 54-55 0.0022381812891154595 1105 56-57 0.009646836653665503 1105 58-59 0.024847888578001687 1105 60-61 0.1518213901354173 1105 62-63 0.0707272682139859 1105 64-65 0.02943589002436653 1105 66-67 0.08171428281293203 1105 68-69 0.08647613457740277 1105 70-71 -0.039760711431867435 1105 72-73 -0.3970294828989793 1105 74-75 -0.2932313286832482 1105 76-77 -0.10723568961616081 1105 78-79 0.02931416262327957 1105 80-81 -0.06105278909732448 1105 82-83 -0.11629764759178585 1105 84-85 -0.13629335084183225 1105 86-87 -0.10764767729877178 1105 88-89 -0.2411670061528035 1105 90-91 -0.3351139379190293 1105 92-93 -0.09691081817581804 1105 94-95 -0.01731406388796941 1105 96-97 -0.19351175326331216 1105 98-99 -0.14636517500859014 1105 100-101 -0.05557700095351237 1106 1 -0.02941121613457298 1106 2 0.03157870936235696 1106 3 0.06316268570532202 1106 4 -0.0026658626720106327 1106 5 -0.04641460143681542 1106 6 0.024915481944312035 1106 7 0.11035397238277511 1106 8 0.002256536474831705 1106 9 0.050131862824486007 1106 10-11 0.11471433418068955 1106 12-13 0.14248849747163916 1106 14-15 0.11081574346901135 1106 16-17 0.10830172154197015 1106 18-19 0.13064473101059093 1106 20-21 0.029207299787238128 1106 22-23 0.047349470434674856 1106 24-25 0.22622036267392787 1106 26-27 0.15811348536134062 1106 28-29 0.1885569643674856 1106 30-31 0.1347744818050458 1106 32-33 0.06846672871398596 1106 34-35 0.08199744802029585 1106 36-37 0.05125248675617655 1106 38-39 0.19214738283313437 1106 40-41 0.2591717975079675 1106 42-43 0.2760849439689679 1106 44-45 0.290975768150318 1106 46-47 0.3792673870748686 1106 48-49 0.29356708668802156 1106 50-51 0.4122751190196894 1106 52-53 0.41927803034362654 1106 54-55 0.42773591334721317 1106 56-57 0.26677165841214645 1106 58-59 0.5180483643188722 1106 60-61 0.37031323565297924 1106 62-63 0.45282063558616414 1106 64-65 0.46799068732907045 1106 66-67 0.404324448440434 1106 68-69 0.2541070439694053 1106 70-71 0.3207323643016906 1106 72-73 0.11508986525859655 1106 74-75 0.30341321416891986 1106 76-77 0.35685200413748674 1106 78-79 0.37724699110923154 1106 80-81 0.44416541293769285 1106 82-83 0.38154033382162567 1106 84-85 0.33629616780389426 1106 86-87 0.30232287924492596 1106 88-89 0.478895945634342 1106 90-91 0.43061271754951846 1106 92-93 0.41697857852367903 1106 94-95 0.39548900046824187 1106 96-97 0.5461975544390896 1106 98-99 0.3444178832825706 1106 100-101 0.4296846027577814 1107 1 -0.02336178489440144 1107 2 0.2615969744136777 1107 3 0.22827065759101828 1107 4 0.12398608405877809 1107 5 0.22864551139034006 1107 6 0.16858167079107034 1107 7 0.2092488211758905 1107 8 0.07735017376327846 1107 9 0.14223511816528145 1107 10-11 0.12787268523960194 1107 12-13 0.2813716705041003 1107 14-15 0.27822996708872694 1107 16-17 0.22704016138290228 1107 18-19 0.25396735192985176 1107 20-21 0.1453198494959267 1107 22-23 0.3257331289938179 1107 24-25 0.3346604348093294 1107 26-27 0.2943690342376897 1107 28-29 0.30227309310141237 1107 30-31 0.2581774458298369 1107 32-33 0.12624209170298428 1107 34-35 0.29857934018712484 1107 36-37 0.19037957914496673 1107 38-39 0.36152798951027876 1107 40-41 0.277848390497887 1107 42-43 0.3481058909771946 1107 44-45 0.2846824175538174 1107 46-47 0.23985244672528694 1107 48-49 0.13436809010982387 1107 50-51 0.11026422934984481 1107 52-53 0.22059588847735512 1107 54-55 0.33338247304387636 1107 56-57 0.2673635385564168 1107 58-59 0.28732146151669014 1107 60-61 0.30102857829609775 1107 62-63 0.25234724707234335 1107 64-65 0.1903370528150603 1107 66-67 0.10090032932455273 1107 68-69 0.1354454791862807 1107 70-71 0.14036613846241863 1107 72-73 0.10984155304182508 1107 74-75 0.026958946158188724 1107 76-77 0.025639574654455544 1107 78-79 0.05624333809896598 1107 80-81 0.04937268658978411 1107 82-83 -0.028942996428995116 1107 84-85 -0.06883827684606558 1107 86-87 -0.009339009222664174 1107 88-89 -0.08345030425005007 1107 90-91 0.12147961387801232 1107 92-93 0.2652324164964881 1107 94-95 0.3828444984798942 1107 96-97 0.424075456039013 1107 98-99 0.4676543810167786 1107 100-101 0.38235904555811473 1108 1 0.08360783728993226 1108 2 0.17539375266011348 1108 3 0.2165200069583335 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-0.3974116180581433 2316 64-65 -0.42624231832512294 2316 66-67 -0.36375369816730796 2316 68-69 -0.4307599461407676 2316 70-71 -0.28994795941924423 2316 72-73 -0.3537184443019612 2316 74-75 -0.28515341190011156 2316 76-77 -0.20085620859808984 2316 78-79 -0.14258875566556384 2316 80-81 -0.2942220906198614 2316 82-83 -0.3180031099823495 2316 84-85 -0.36379270609103287 2316 86-87 -0.2741640308044353 2316 88-89 -0.2843301767515527 2316 90-91 -0.19286106279374593 2316 92-93 -0.06756732120189923 2316 94-95 0.0974121988584038 2316 96-97 0.025602366856464442 2316 98-99 -0.01139759786627792 2316 100-101 -0.059996439533854584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8669.0 3 1011.0 4 703.0 5 688.0 6 773.0 7 869.0 8 1042.0 9 1198.0 10 1258.0 11 1520.0 12 1856.0 13 2203.0 14 2531.0 15 3109.0 16 3532.0 17 4692.0 18 4256.0 19 2695.0 20 2579.0 21 2703.0 22 2774.0 23 3136.0 24 3606.0 25 4149.0 26 5022.0 27 5989.0 28 7181.0 29 9064.0 30 11457.0 31 14714.0 32 19093.0 33 25783.0 34 37484.0 35 59567.0 36 103980.0 37 204564.0 38 317576.0 39 135062.0 40 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.608041580066114 21.252831628864072 15.03382216534647 23.10530462572334 2 15.71275822417119 22.42390618990426 39.559534805485306 22.303800780439246 3 17.687936663128937 26.783906895035603 32.322047018715224 23.20610942312024 4 13.398929399798941 19.619899550080362 38.47816207730276 28.503008972817927 5 13.601063024110818 37.34481992127642 35.256933557058865 13.797183497553902 6 28.151323306998965 37.929359380562126 19.63631428959153 14.283003022847376 7 24.111229340691978 33.85618781063948 24.77956520798463 17.25301764068391 8 23.42236303349971 34.8856990021279 22.846236444947905 18.84570151942448 9 24.711823708774542 17.47474677949536 23.19136990301859 34.62205960871152 10-11 22.970743548626547 27.01514783567936 30.37088453646561 19.64322407922848 12-13 23.671685704395983 25.87026810157651 29.568710862519275 20.889335331508228 14-15 21.57242944810015 27.939324407192906 27.37861709572103 23.109629048985912 16-17 20.738256188815747 30.21450298897608 28.30455834220641 20.74268248000177 18-19 20.76514771862716 28.957404963904555 30.052587860776182 20.224859456692105 20-21 21.681094108754866 28.10412936456145 29.727235040513712 20.487541486169967 22-23 21.78494403702476 28.081749903868186 29.106008557944126 21.027297501162927 24-25 21.323845732915167 28.536450940606787 29.168373113833013 20.971330212645032 26-27 21.008828515574297 28.5467956559534 29.680548945258877 20.76382688321343 28-29 21.068484892886485 28.356107872200344 29.698536270200716 20.876870964712456 30-31 21.45803885514254 28.10977573360407 29.414468783877794 21.01771662737559 32-33 21.20460508817307 28.492766263309292 29.23814079668202 21.064487851835608 34-35 21.390892077600355 28.547151942614995 29.14817411793862 20.913781861846033 36-37 21.471934698694465 28.406117080126865 29.25193617387456 20.870012047304108 38-39 21.246111555520375 28.490627171002075 29.32832360904681 20.934937664430745 40-41 21.26682393756103 28.480306884748096 29.229769236593704 21.023099941097165 42-43 21.490157864539057 28.407406442312748 28.831947939953555 21.27048775319463 44-45 21.465444160115183 28.50244586543511 28.880389607579726 21.15172036686998 46-47 21.513442705495684 28.56552790327625 28.814831132711927 21.10619825851614 48-49 21.266171016149134 28.65135717032667 28.900739738234364 21.18173207528983 50-51 21.361514351942457 28.589509957898606 28.700787053109124 21.348188637049816 52-53 21.514551249810097 28.701506281622596 28.46934706819099 21.314595400376312 54-55 21.57628309343517 28.588824591581545 28.41263106974147 21.422261245241817 56-57 21.406981952856057 28.808803926186823 28.41479862852917 21.369415492427947 58-59 21.385516104798203 28.72250148481166 28.528640148520502 21.363342261869644 60-61 21.384705319553245 28.56443303150669 28.76586384417702 21.28499780476305 62-63 21.313712861307913 28.69749696400564 28.572222249536612 21.41656792514983 64-65 21.340698497248884 28.643393260117715 28.42790664738335 21.58800159525005 66-67 21.389817407235853 28.693527973069855 28.43650950530706 21.48014511438723 68-69 21.29111760419115 28.57045915211215 28.76184219455178 21.376581049144924 70-71 21.347977610736777 28.534313551955126 28.724693091661457 21.39301574564664 72-73 21.339210644062128 28.491304497470267 28.438644711157878 21.73084014730973 74-75 21.294470508015962 28.753436974744474 28.363097915618923 21.588994601620644 76-77 21.321287358266137 28.631482362035214 28.531711162130414 21.51551911756824 78-79 21.230496889828636 28.752145157721337 28.457846728788137 21.55951122366189 80-81 21.336045070167668 28.575566143087062 28.433209793920128 21.655178992825142 82-83 21.307155987123767 28.76513267181834 28.325893010817726 21.601818330240167 84-85 21.49478070298367 28.458285206836987 28.35000980969198 21.696924280487366 86-87 21.197162978272875 28.74975227939835 28.402132044546924 21.65095269778185 88-89 21.251611520144127 28.59434141314214 28.472549333905008 21.68149773280873 90-91 21.335037341982538 28.623411465965393 28.454059625223 21.58749156682907 92-93 21.396671645248276 28.560075185270613 28.389029678215106 21.654223491266006 94-95 21.371230041759084 28.529178502012726 28.453458510607437 21.646132945620757 96-97 21.3042091037225 28.37213281018401 28.44105794676836 21.882600139325135 98-99 21.207899072325567 28.516610775457952 28.369856406872923 21.90563374534356 100-101 21.285145302827758 28.448722753568017 28.429049351712226 21.837082591892003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1877.0 1 1063.0 2 248.5 3 222.0 4 162.5 5 150.5 6 205.0 7 335.5 8 548.5 9 639.0 10 531.5 11 394.5 12 380.5 13 419.0 14 437.5 15 526.0 16 579.5 17 606.5 18 700.5 19 777.5 20 850.0 21 938.5 22 1045.5 23 1291.0 24 1630.0 25 2146.0 26 3266.0 27 5152.0 28 7774.5 29 12013.0 30 18475.0 31 25395.5 32 31463.5 33 37087.5 34 41972.5 35 45144.0 36 47588.5 37 47948.5 38 44043.0 39 39985.5 40 37211.0 41 33689.0 42 31376.5 43 30134.0 44 28606.0 45 27720.5 46 27346.5 47 27039.5 48 27435.5 49 27926.0 50 27466.5 51 26763.5 52 26316.0 53 26097.0 54 25195.5 55 23448.0 56 21615.0 57 19729.0 58 17897.0 59 16101.0 60 13856.0 61 11509.5 62 9463.0 63 7371.5 64 5467.5 65 4118.5 66 3095.5 67 2281.0 68 1689.0 69 1258.5 70 976.0 71 742.5 72 541.5 73 419.5 74 307.0 75 213.5 76 172.0 77 123.0 78 80.5 79 59.0 80 39.0 81 29.0 82 26.5 83 23.5 84 18.0 85 9.0 86 7.0 87 7.5 88 7.5 89 9.5 90 9.0 91 6.5 92 5.0 93 5.5 94 4.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1475234814755884 2 0.15086289450499585 3 0.145755556930608 4 0.14899675192973874 5 0.13956782102317652 6 0.1199242149678385 7 0.12660304102665343 8 0.11579905769621754 9 0.11236142663653337 10-11 0.10155744330609746 12-13 0.11933490678617836 14-15 0.13667038913001414 16-17 0.14639397412740648 18-19 0.14374208730993582 20-21 0.1363757350391841 22-23 0.12866561966246393 24-25 0.11457133231775891 26-27 0.1189420346650716 28-29 0.12321451898210763 30-31 0.11398202413609876 32-33 0.1117230094397349 34-35 0.1268976951174835 36-37 0.12945136390467743 38-39 0.11663391095356938 40-41 0.11923668875590168 42-43 0.11427667822692883 44-45 0.13013889011661428 46-47 0.12856740163218724 48-49 0.1406482193562201 50-51 0.1288620557230173 52-53 0.11476776837831228 54-55 0.10602636368368687 56-57 0.12557175170874818 58-59 0.11579905769621754 60-61 0.11476776837831228 62-63 0.11570083966594084 64-65 0.13500068261531042 66-67 0.1267012590569301 68-69 0.13102285238910447 70-71 0.12051352314949866 72-73 0.12159392148254222 74-75 0.10592814565341017 76-77 0.1293040368592624 78-79 0.12945136390467743 80-81 0.13048265322258268 82-83 0.13613018996349235 84-85 0.12684858610234515 86-87 0.13480424655475703 88-89 0.12237966572475574 90-91 0.13013889011661428 92-93 0.1428581250374456 94-95 0.12164303049768059 96-97 0.1077942882286673 98-99 0.1117230094397349 100-101 0.15145220268665602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1018143.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.767821154037755 #Duplication Level Percentage of deduplicated Percentage of total 1 61.28965321864646 29.276731935412982 2 19.166803930728555 18.311129245150994 3 8.144316843600548 11.671088112207846 4 3.9770409616140507 7.598983255066492 5 2.2559876786173083 5.3881807978952185 6 1.3338276421727877 3.8228424156972944 7 0.8739862688845121 2.922389384821209 8 0.6169295172769647 2.3575503076746314 9 0.44646263848031964 1.919389272019895 >10 1.8415468238115733 14.104666072517002 >50 0.04143371022715374 1.32429091626412 >100 0.01118243751932297 0.8158808887569887 >500 4.1416421022860575E-4 0.15104871687907642 >1k 4.1416421022860575E-4 0.33582867963619156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1751 0.17197977101448422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1644 0.16147044177487838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.9643606055338002E-4 10-11 0.0 0.0 0.0 0.0 1.9643606055338002E-4 12-13 0.0 0.0 0.0 0.0 1.9643606055338002E-4 14-15 0.0 0.0 0.0 9.821803027669001E-5 1.9643606055338002E-4 16-17 0.0 0.0 0.0 9.821803027669001E-5 1.9643606055338002E-4 18-19 0.0 0.0 0.0 9.821803027669001E-5 1.9643606055338002E-4 20-21 0.0 0.0 0.0 3.9287212110676004E-4 1.9643606055338002E-4 22-23 0.0 0.0 0.0 8.839622724902102E-4 2.4554507569172503E-4 24-25 0.0 0.0 0.0 0.0012768343935969702 2.9465409083007E-4 26-27 0.0 0.0 0.0 0.00216079666608718 2.9465409083007E-4 28-29 0.0 0.0 0.0 0.0034867400748224957 2.9465409083007E-4 30-31 0.0 0.0 0.0 0.005893081816601401 2.9465409083007E-4 32-33 0.0 0.0 0.0 0.013554088178183222 2.9465409083007E-4 34-35 0.0 0.0 0.0 0.029072536961900243 2.9465409083007E-4 36-37 0.0 0.0 0.0 0.04660445536628941 2.9465409083007E-4 38-39 0.0 0.0 0.0 0.06300686642249664 2.9465409083007E-4 40-41 0.0 0.0 0.0 0.08014591270577906 2.9465409083007E-4 42-43 0.0 0.0 0.0 0.10229407853317266 2.9465409083007E-4 44-45 0.0 0.0 0.0 0.13274166791894657 2.9465409083007E-4 46-47 0.0 0.0 0.0 0.16525183594053094 2.9465409083007E-4 48-49 0.0 0.0 0.0 0.19997190964334088 2.9465409083007E-4 50-51 0.0 0.0 0.0 0.23459376531587411 2.9465409083007E-4 52-53 0.0 0.0 0.0 0.27609088310777563 2.9465409083007E-4 54-55 0.0 0.0 0.0 0.32750802195762285 2.9465409083007E-4 56-57 0.0 0.0 0.0 0.38609507701766843 2.9465409083007E-4 58-59 0.0 0.0 0.0 0.4394274674579111 2.9465409083007E-4 60-61 0.0 0.0 0.0 0.4961483799426996 2.9465409083007E-4 62-63 0.0 0.0 0.0 0.565097437196936 2.9465409083007E-4 64-65 0.0 0.0 0.0 0.6575697127024396 2.9465409083007E-4 66-67 0.0 0.0 0.0 0.7722392630504753 2.9465409083007E-4 68-69 0.0 4.9109015138345005E-5 0.0 0.8862703962017123 2.9465409083007E-4 70-71 0.0 9.821803027669001E-5 0.0 0.9941629024606563 2.9465409083007E-4 72-73 0.0 9.821803027669001E-5 0.0 1.113694245307388 3.9287212110676004E-4 74-75 0.0 9.821803027669001E-5 0.0 1.2622981251160201 3.9287212110676004E-4 76-77 0.0 9.821803027669001E-5 0.0 1.4192996465133092 3.9287212110676004E-4 78-79 0.0 9.821803027669001E-5 0.0 1.5866140610896504 3.9287212110676004E-4 80-81 0.0 9.821803027669001E-5 0.0 1.7546160018779289 4.910901513834501E-4 82-83 0.0 9.821803027669001E-5 0.0 1.9405918422068413 5.8930818166014E-4 84-85 0.0 9.821803027669001E-5 0.0 2.1495998106356375 5.8930818166014E-4 86-87 0.0 9.821803027669001E-5 0.0 2.3870418988295357 5.8930818166014E-4 88-89 0.0 9.821803027669001E-5 0.0 2.6699589350415414 5.8930818166014E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1615 0.0 52.654552 1 GTATCAA 2645 0.0 37.53842 1 TCAACGC 3025 0.0 32.19308 4 CAACGCA 3085 0.0 31.56696 5 ATCAACG 3145 0.0 30.966251 3 TATCAAC 3165 0.0 30.920671 2 AACGCAG 3175 0.0 30.518026 6 GTGGTAT 840 0.0 29.974504 1 TGGTATC 925 0.0 27.22009 2 ACGCAGA 3690 0.0 26.002575 7 CGCAGAG 3745 0.0 25.875639 8 GCAGAGT 4095 0.0 22.504044 9 CAGAGTA 3930 0.0 20.850666 10-11 GAGTACT 2200 0.0 20.297346 12-13 GTACATG 2535 0.0 19.115147 1 AGAGTAC 3505 0.0 18.635372 10-11 TACATGG 2545 0.0 18.293371 2 CTATAAC 515 0.0 17.526766 3 CGGAGAT 190 1.8633816E-4 17.502491 1 ACATGGG 2425 0.0 17.043663 3 >>END_MODULE