FastQCFastQC Report
Fri 10 Feb 2017
SRR2031681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018143
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT39530.38825587368375564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22030.21637432069954812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.21313312570041734No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT15620.1534165632921898RNA PCR Primer, Index 33 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.10047704497305389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.058.3800771
GTATCAA29700.039.827721
CGGAGAT1404.5474735E-1033.9323961
TCAACGC35950.032.637684
TATCAAC36250.032.629662
ATCAACG36100.032.5020683
CAACGCA37000.031.708365
AACGCAG37300.031.4533356
GTGGTAT8350.031.290951
TGGTATC8350.030.7205162
ACGCAGA41850.028.0336787
CGCAGAG42900.027.12618
CGTCAGA2801.8189894E-1223.751512
GCAGAGT47150.023.6736249
GAGTACT26800.023.48332812-13
CAGAGTA46800.021.05963310-11
GTACTTT31300.019.65187614-15
AGAGTAC42300.019.31375110-11
AGTACTT32150.017.2855612-13
TACTCCG1800.002618939615.8327855
Produced by FastQC (version 0.11.5)