##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031681_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1018143 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.736662728123655 34.0 31.0 34.0 31.0 34.0 2 32.90530996137085 34.0 31.0 34.0 31.0 34.0 3 32.958434129586905 34.0 31.0 34.0 31.0 34.0 4 36.36403432523722 37.0 37.0 37.0 35.0 37.0 5 36.329823020931244 37.0 37.0 37.0 35.0 37.0 6 36.35556891320768 37.0 37.0 37.0 35.0 37.0 7 36.304162578341156 37.0 37.0 37.0 35.0 37.0 8 36.29953159821361 37.0 37.0 37.0 35.0 37.0 9 38.1466395192031 39.0 39.0 39.0 37.0 39.0 10-11 38.11161005870491 39.0 39.0 39.0 36.5 39.0 12-13 38.06281976107482 39.0 39.0 39.0 35.0 39.0 14-15 39.55368155553788 41.0 39.0 41.0 37.0 41.0 16-17 39.53011364808283 41.0 39.0 41.0 37.0 41.0 18-19 39.538352667552594 41.0 39.5 41.0 37.0 41.0 20-21 39.532085866130785 41.0 39.5 41.0 37.0 41.0 22-23 39.44192024106633 41.0 39.0 41.0 36.0 41.0 24-25 39.378248438578865 41.0 39.0 41.0 36.0 41.0 26-27 39.25346930637445 41.0 39.0 41.0 36.0 41.0 28-29 39.15182985101307 40.5 39.0 41.0 36.0 41.0 30-31 38.99497516557105 40.0 39.0 41.0 35.0 41.0 32-33 38.808402159618055 40.0 38.0 41.0 35.0 41.0 34-35 38.67396966830789 40.0 38.0 41.0 35.0 41.0 36-37 38.5755375227252 40.0 38.0 41.0 35.0 41.0 38-39 38.64349556005394 40.0 38.0 41.0 35.0 41.0 40-41 38.58553759147782 40.0 38.0 41.0 35.0 41.0 42-43 38.49149922947955 40.0 38.0 41.0 35.0 41.0 44-45 38.3211100994654 40.0 38.0 41.0 34.0 41.0 46-47 38.13900012080818 40.0 38.0 41.0 34.0 41.0 48-49 37.92544907738893 40.0 38.0 41.0 33.0 41.0 50-51 37.777524866349815 40.0 38.0 41.0 33.0 41.0 52-53 37.83826289627292 40.0 38.0 41.0 33.0 41.0 54-55 37.770525849512296 40.0 37.5 41.0 33.0 41.0 56-57 37.59964513825661 40.0 37.0 41.0 33.0 41.0 58-59 37.41998275291388 40.0 37.0 41.0 33.0 41.0 60-61 37.169456058726524 40.0 36.0 41.0 33.0 41.0 62-63 36.90168129624227 39.0 36.0 41.0 32.0 41.0 64-65 36.586512896518464 39.0 35.0 41.0 32.0 41.0 66-67 36.22981644032322 38.5 35.0 41.0 31.0 41.0 68-69 35.835658645200134 38.0 35.0 40.0 31.0 41.0 70-71 35.50817910647129 37.0 35.0 40.0 31.0 41.0 72-73 35.14694890599847 37.0 35.0 39.5 30.0 41.0 74-75 34.78222656345916 36.0 35.0 39.0 30.0 41.0 76-77 33.845241778414234 35.0 33.5 38.0 28.5 39.0 78-79 34.06326419766182 35.0 34.0 37.5 29.0 39.0 80-81 33.832954211736464 35.0 34.0 37.0 29.5 39.0 82-83 33.537877292286055 35.0 34.0 37.0 29.0 39.0 84-85 33.21587242656483 35.0 34.0 36.0 29.0 37.5 86-87 32.93763105968415 35.0 34.0 36.0 29.0 37.0 88-89 32.68283531881082 35.0 34.0 35.5 29.0 37.0 90-91 32.463467803638586 35.0 34.0 35.0 29.0 36.0 92-93 32.28225455559779 35.0 34.0 35.0 28.5 36.0 94-95 32.090504968359056 35.0 34.0 35.0 27.0 36.0 96-97 31.917044560538155 35.0 34.0 35.0 27.0 36.0 98-99 31.70687172627028 35.0 33.5 35.0 26.5 35.0 100-101 30.98693061780123 34.5 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25832245964836886 1101 2 0.18499914944762708 1101 3 0.2635911345233879 1101 4 0.12701843839956695 1101 5 0.11447111137883326 1101 6 0.05704660444200016 1101 7 0.2207574411410249 1101 8 0.21094070945992627 1101 9 0.19687553895411014 1101 10-11 0.11559214952693253 1101 12-13 0.21912997587798344 1101 14-15 0.17810992371433088 1101 16-17 0.04394217875060491 1101 18-19 0.11315304310428331 1101 20-21 0.05321069133129441 1101 22-23 0.11008142637775364 1101 24-25 0.10757421843946702 1101 26-27 0.10335613384530973 1101 28-29 0.06019705003691911 1101 30-31 0.13676713572994714 1101 32-33 0.2890167158340162 1101 34-35 -0.03491681525869694 1101 36-37 0.08511741830783848 1101 38-39 0.09284510235968213 1101 40-41 -0.0817539680942474 1101 42-43 0.05793432875877613 1101 44-45 0.16252209850535593 1101 46-47 0.2638180259054792 1101 48-49 -0.06793850273420077 1101 50-51 0.056523008123647855 1101 52-53 0.03931558392177692 1101 54-55 0.17519845594959094 1101 56-57 -0.11064108358405633 1101 58-59 0.06361426534579806 1101 60-61 0.0882242264524038 1101 62-63 0.2978701856311261 1101 64-65 0.21290748854701036 1101 66-67 -0.11074684448281857 1101 68-69 -0.14394471233678985 1101 70-71 0.13197953492474568 1101 72-73 0.10847236805593496 1101 74-75 0.057493873437785226 1101 76-77 -0.01790727217773025 1101 78-79 -0.04110444219156051 1101 80-81 -0.004535228905396593 1101 82-83 0.05697500857444027 1101 84-85 0.08598586753907966 1101 86-87 0.3824270462653274 1101 88-89 0.13812030472064407 1101 90-91 -0.10990906102594522 1101 92-93 -0.15509902692533473 1101 94-95 -0.023724583746293604 1101 96-97 -0.12200632985179638 1101 98-99 -0.27211715589076846 1101 100-101 -0.41178664472145954 1102 1 0.1930683028787783 1102 2 0.16534933607810132 1102 3 0.16284596994924527 1102 4 0.09312498743248199 1102 5 0.08777005946163285 1102 6 0.11375104964051275 1102 7 0.1563665351349144 1102 8 -0.045742018738238244 1102 9 0.04786434112580906 1102 10-11 0.20100986245533647 1102 12-13 0.2142070033635619 1102 14-15 0.16967897698884826 1102 16-17 0.13788313565593313 1102 18-19 0.15780726773949993 1102 20-21 0.23614927293970567 1102 22-23 0.21520392383279585 1102 24-25 0.23950771012593464 1102 26-27 0.24608772529423817 1102 28-29 0.369356873586284 1102 30-31 0.11114650457962938 1102 32-33 0.3641799326646904 1102 34-35 0.37648257395066764 1102 36-37 0.43330133415446426 1102 38-39 0.20361877049676735 1102 40-41 0.3160397204025287 1102 42-43 0.3448939487112739 1102 44-45 0.3951946468596219 1102 46-47 0.30742440527433246 1102 48-49 0.2764286503027833 1102 50-51 0.41024543771306554 1102 52-53 0.4347869106947613 1102 54-55 0.5460325244938744 1102 56-57 0.42291643253403066 1102 58-59 0.37443815404617453 1102 60-61 0.3429646404327187 1102 62-63 0.38014979201049215 1102 64-65 0.24171033890330307 1102 66-67 0.2687523071968556 1102 68-69 0.3190854878668077 1102 70-71 0.364969015752699 1102 72-73 0.2827865859045957 1102 74-75 0.24230893960677236 1102 76-77 0.2262691784500248 1102 78-79 0.3436590461016138 1102 80-81 0.2700613099476783 1102 82-83 0.3176611300539065 1102 84-85 0.40919794762051964 1102 86-87 0.4202838497943375 1102 88-89 0.33579794299685517 1102 90-91 0.19364031121700975 1102 92-93 0.20079238807296917 1102 94-95 0.1380480664064052 1102 96-97 -0.04880035767670421 1102 98-99 -0.10399499776386278 1102 100-101 0.002758994912067436 1103 1 0.2569226828560218 1103 2 0.23653282380766427 1103 3 0.19403465839390321 1103 4 0.21157387415441065 1103 5 0.196125658356479 1103 6 0.2537165949029827 1103 7 0.13934069408176697 1103 8 0.19873319660386812 1103 9 0.2755035415158602 1103 10-11 0.2426441871749816 1103 12-13 0.2213404342102976 1103 14-15 0.3135444084856758 1103 16-17 0.30302924324573155 1103 18-19 0.36320235956463165 1103 20-21 0.3769131279697788 1103 22-23 0.2865505839985687 1103 24-25 0.21771476359577946 1103 26-27 0.3352117298448647 1103 28-29 0.34743975998316046 1103 30-31 0.4397985442350816 1103 32-33 0.37506177891854975 1103 34-35 0.3009005814266956 1103 36-37 0.27434602779984374 1103 38-39 0.18934930844573472 1103 40-41 0.12461244915272829 1103 42-43 0.3637335391549641 1103 44-45 0.5038629387497551 1103 46-47 0.2842012620921395 1103 48-49 0.228744593745013 1103 50-51 0.04577607317543908 1103 52-53 0.038639340403854305 1103 54-55 0.3163799972713832 1103 56-57 0.2538028294136012 1103 58-59 0.20742173928914553 1103 60-61 0.07313203844994831 1103 62-63 -0.021145836138373397 1103 64-65 0.045551067003415824 1103 66-67 -0.009537307625187452 1103 68-69 0.001971884746382102 1103 70-71 -0.047493197801664166 1103 72-73 -0.09694622735722902 1103 74-75 -0.027006841313102825 1103 76-77 0.007300870307659579 1103 78-79 -0.055455532152898 1103 80-81 0.012290466456697402 1103 82-83 -0.015120927479003399 1103 84-85 -0.09040289749568586 1103 86-87 -7.523926890016241E-4 1103 88-89 0.06418344600351844 1103 90-91 0.006749363711733736 1103 92-93 0.12338494454948545 1103 94-95 0.013400577376664558 1103 96-97 0.1013033315805636 1103 98-99 0.15598484946513125 1103 100-101 0.11987519839004435 1104 1 0.1226976132236004 1104 2 0.02451559223573696 1104 3 0.10276715221525023 1104 4 -0.0038097416315565624 1104 5 0.1090422269493132 1104 6 0.05760203486218529 1104 7 0.014075894740827266 1104 8 0.08609172510412577 1104 9 0.058647592357331746 1104 10-11 0.14002340925336654 1104 12-13 0.1567784319349883 1104 14-15 0.1811980656587977 1104 16-17 0.17712389920272642 1104 18-19 0.043378696310931275 1104 20-21 0.22611848476236673 1104 22-23 0.1985893918130941 1104 24-25 0.2155939170224883 1104 26-27 0.2470360011563102 1104 28-29 0.2793642627488495 1104 30-31 0.2812468740377554 1104 32-33 0.16225444005385015 1104 34-35 0.1881371306009143 1104 36-37 0.17744172824313154 1104 38-39 0.057128622713513266 1104 40-41 0.10981065636311627 1104 42-43 0.2176525694009257 1104 44-45 0.1446638586822786 1104 46-47 0.17073351857482066 1104 48-49 0.17339601483480038 1104 50-51 0.17586120125986326 1104 52-53 0.2412721323696374 1104 54-55 0.2576913914889545 1104 56-57 0.24296076750939477 1104 58-59 0.34445601118903113 1104 60-61 0.49337104437361035 1104 62-63 0.44435792008930974 1104 64-65 0.44066723545502384 1104 66-67 0.27732496729537814 1104 68-69 0.48304792629046034 1104 70-71 0.46400534580195796 1104 72-73 0.3289577681706106 1104 74-75 0.42115746177425706 1104 76-77 0.3951601883022491 1104 78-79 0.42163564708683765 1104 80-81 0.48375465970137554 1104 82-83 0.31757553892090584 1104 84-85 0.4484208540244552 1104 86-87 0.4191212882179798 1104 88-89 0.3920467114697601 1104 90-91 0.45194080382785273 1104 92-93 0.33598081171828653 1104 94-95 0.4488097659137935 1104 96-97 0.45426552902280193 1104 98-99 0.38217987908342366 1104 100-101 0.521372295404678 1105 1 0.13310212951725475 1105 2 0.04100744541062795 1105 3 -0.005638475837528745 1105 4 -0.04263701800394415 1105 5 0.015720527598148237 1105 6 -0.029983169628593487 1105 7 0.03174557470000394 1105 8 0.1375174194979465 1105 9 0.012910550730154569 1105 10-11 0.04201318471447024 1105 12-13 0.14567906618446358 1105 14-15 0.10625342579584895 1105 16-17 0.07673182185345695 1105 18-19 -0.013968026733472527 1105 20-21 0.016786542646748615 1105 22-23 0.011761459320560164 1105 24-25 0.17963033169041864 1105 26-27 -0.11262263411379081 1105 28-29 0.10909652927942659 1105 30-31 0.11810875163489953 1105 32-33 -0.2643546490326756 1105 34-35 -0.07938332758965316 1105 36-37 -0.032275228817447044 1105 38-39 -0.04414625517523518 1105 40-41 -0.0986760754295446 1105 42-43 -0.0505183134451741 1105 44-45 -0.16601586356019737 1105 46-47 -0.13386342317629385 1105 48-49 -0.19580028355405688 1105 50-51 -0.11767966044459399 1105 52-53 -0.1107135423405694 1105 54-55 -0.2591695292663161 1105 56-57 -0.2548612781274002 1105 58-59 -0.19871920022745826 1105 60-61 -0.22811962413991438 1105 62-63 -0.3809387008919529 1105 64-65 -0.31302116215378106 1105 66-67 -0.11609879518913147 1105 68-69 -0.2757197069383821 1105 70-71 -0.26408867074684395 1105 72-73 -0.30673380068556355 1105 74-75 -0.295277892166105 1105 76-77 -0.170557762051331 1105 78-79 -0.09379912363562681 1105 80-81 -0.266202054594757 1105 82-83 -0.2673270910273402 1105 84-85 -0.1494936863269274 1105 86-87 -0.22690189469373223 1105 88-89 -0.13029326811761166 1105 90-91 -0.12432405000316749 1105 92-93 -0.16520157145587433 1105 94-95 -0.039442721875083464 1105 96-97 -0.01938145100198696 1105 98-99 -0.013064161158609977 1105 100-101 0.05722652737960132 1106 1 0.09994114112902963 1106 2 0.010107946745158358 1106 3 0.02775048964115001 1106 4 0.058686969702499425 1106 5 0.005983109890344451 1106 6 0.041322042228799205 1106 7 -0.04208136487962122 1106 8 -0.04357489186714503 1106 9 -0.05431150540812979 1106 10-11 -0.01200394367749169 1106 12-13 -0.003699696244055417 1106 14-15 0.060684354114265204 1106 16-17 0.11236184514583414 1106 18-19 0.1838924297799025 1106 20-21 0.19367579524751477 1106 22-23 0.17478712394905216 1106 24-25 0.22555857482583264 1106 26-27 0.08096056354449388 1106 28-29 0.16624511657422403 1106 30-31 0.16801207644417815 1106 32-33 0.23321234074491315 1106 34-35 0.25411489483425953 1106 36-37 0.22426136903311544 1106 38-39 0.16988820807851113 1106 40-41 0.2746934044600735 1106 42-43 0.19547380629011002 1106 44-45 0.19226949063232723 1106 46-47 0.31031875202625514 1106 48-49 0.4415499989439695 1106 50-51 0.37418091220804683 1106 52-53 0.25902873601085474 1106 54-55 -0.10385906103147846 1106 56-57 0.08204853542604695 1106 58-59 0.028761072226672013 1106 60-61 0.24485971382321736 1106 62-63 0.3145860658079016 1106 64-65 0.34349263798773677 1106 66-67 0.2752147399827862 1106 68-69 0.2671453628849818 1106 70-71 0.1732439757875781 1106 72-73 0.3283625196351565 1106 74-75 0.26146313231132723 1106 76-77 0.304321205771636 1106 78-79 0.23665366897619577 1106 80-81 0.09897745585670492 1106 82-83 0.14684060978068914 1106 84-85 0.2509814093266627 1106 86-87 0.06529591199459617 1106 88-89 0.016136847225894257 1106 90-91 0.23282356946435812 1106 92-93 0.1711780878259077 1106 94-95 0.1662689645316746 1106 96-97 0.14744669943708644 1106 98-99 0.37474530161122743 1106 100-101 0.41732548234121936 1107 1 0.09779557560604957 1107 2 -0.014806508077739977 1107 3 0.06542389202716237 1107 4 0.06204057184806544 1107 5 0.013685643877217046 1107 6 0.007543257432715222 1107 7 0.07971069097906991 1107 8 0.06405018905396531 1107 9 0.08066953593100123 1107 10-11 0.13140271959819216 1107 12-13 0.12041479557135659 1107 14-15 0.16024391205377952 1107 16-17 -0.012736994214193942 1107 18-19 1.9687812910973435E-4 1107 20-21 0.028097324887760067 1107 22-23 0.14590522253408977 1107 24-25 0.19482589194412014 1107 26-27 0.24131499802150813 1107 28-29 0.11613934111875324 1107 30-31 0.1551594915926202 1107 32-33 0.11342019240495205 1107 34-35 0.1741578986259924 1107 36-37 0.17560003545316505 1107 38-39 0.12756357569157473 1107 40-41 0.2720559541687635 1107 42-43 0.2246666759839968 1107 44-45 0.28811997153494673 1107 46-47 0.2547068728067927 1107 48-49 0.18706176718590228 1107 50-51 0.3482162169338423 1107 52-53 0.4506765210843753 1107 54-55 0.3134123946237466 1107 56-57 0.39599760136519535 1107 58-59 0.2901946475526671 1107 60-61 0.48020490017298556 1107 62-63 0.4573673244780281 1107 64-65 0.36639215708512296 1107 66-67 0.4009730864176362 1107 68-69 0.3873484748797935 1107 70-71 0.420147587181269 1107 72-73 0.3058190534370624 1107 74-75 0.3419842642820967 1107 76-77 0.3750289187097664 1107 78-79 0.592207747400316 1107 80-81 0.5658275437139082 1107 82-83 0.6558494417422196 1107 84-85 0.6145258696984399 1107 86-87 0.48904683680732575 1107 88-89 0.5041940468929624 1107 90-91 0.39765005147675225 1107 92-93 0.42857485560543296 1107 94-95 0.37914871575705433 1107 96-97 0.49841717479082703 1107 98-99 0.6493037035348408 1107 100-101 0.7668856182886934 1108 1 0.027634936639877594 1108 2 0.031267260207279435 1108 3 -0.03279808589619648 1108 4 0.018121743884321972 1108 5 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0.02442853181389637 2316 96-97 -0.0970245046035565 2316 98-99 -0.10107148738089222 2316 100-101 0.1296582957573129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 5.0 5 9.0 6 14.0 7 58.0 8 155.0 9 256.0 10 526.0 11 773.0 12 1091.0 13 1605.0 14 2050.0 15 2545.0 16 3154.0 17 4478.0 18 4560.0 19 2837.0 20 2256.0 21 2475.0 22 3083.0 23 3536.0 24 4329.0 25 5207.0 26 5504.0 27 5273.0 28 6747.0 29 8391.0 30 11124.0 31 14624.0 32 18864.0 33 25241.0 34 35521.0 35 56671.0 36 100320.0 37 210601.0 38 337661.0 39 136521.0 40 75.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.03518847248432 21.628827434041263 15.418975644115068 22.917008449359347 2 15.455392808279386 22.490259226847307 39.98917637306351 22.065171591809797 3 17.470251688152242 26.78728054020872 32.94340085942296 22.799066912216084 4 13.156475482707977 19.888010766684186 38.33774577461676 28.617767975991082 5 13.172707566618836 37.547181486294164 35.708834613605354 13.571276333481643 6 27.9337971188723 38.162124573856524 19.469858359778538 14.434219947492641 7 23.76886154498926 33.88138994227726 24.637600022786582 17.712148489946895 8 23.103827261985792 35.29307769144413 22.947365939755024 18.655729106815055 9 24.356794674225526 17.141796388130153 23.00953795292017 35.49187098472415 10-11 22.66967672485763 27.082322505112256 30.510577110069125 19.73742365996099 12-13 23.51571612028768 25.631137095249436 29.84292473794189 21.010222046520994 14-15 21.24549302013568 27.767072012477616 27.30696964964646 23.68046531774024 16-17 20.532110443168364 30.19650154277259 28.445210333212362 20.826177680846687 18-19 20.555187783236523 28.6569064040183 30.409216003268703 20.378689809476477 20-21 21.36345287449798 27.823350943826163 30.042243574822002 20.77095260685385 22-23 21.347443335562883 28.159354825402716 29.419050172716403 21.07415166631799 24-25 21.11874265206361 28.624122544672016 29.47886495315491 20.778269850109467 26-27 20.850601367102804 28.70606503928493 29.96576595423904 20.477567639373227 28-29 20.787440863848232 28.387415522414155 30.037850083808443 20.787293529929165 30-31 21.377803205346993 28.024171469121463 29.8028026528703 20.795222672661243 32-33 20.99174917410711 28.488356960557017 29.622777995767773 20.8971158695681 34-35 21.236431468139582 28.2376244626245 29.66656565423395 20.859378415001967 36-37 21.029851743152427 28.440140340124426 29.763735907545247 20.7662720091779 38-39 20.8508038654688 28.22079020334079 29.997652589076385 20.930753342114023 40-41 20.94897278673035 27.969548481893014 30.000697348014967 21.080781383361668 42-43 21.310022266027463 27.94415912106649 29.711347030819834 21.03447158208621 44-45 21.0739061212423 28.3675770495893 29.421260078397633 21.137256750770767 46-47 21.40210166941186 28.1265500032903 29.288960391614932 21.182387935682907 48-49 21.170208899928593 28.202521649709322 29.265879154499906 21.361390295862172 50-51 21.094139035479298 28.293226000669847 29.2210426236786 21.391592340172256 52-53 21.279672894671968 28.544467722117624 29.0838811443972 21.091978238813212 54-55 21.143984685844718 28.491184440692514 28.88273061839054 21.482100255072226 56-57 21.239747265364493 28.69100902329044 28.775034548192153 21.294209163152917 58-59 20.91183031000679 28.72078370438614 29.25715447497837 21.110231510628704 60-61 21.20038384624149 28.915762054618533 28.907855293502898 20.97599880563708 62-63 20.966886402414048 29.144847800825314 28.668344398703 21.219921398057643 64-65 21.017709110910303 29.124366479393395 28.680312733273087 21.17761167642321 66-67 21.0401365305045 29.035716478200545 28.661681829901653 21.262465161393298 68-69 20.896575703732516 28.98153141074563 28.809250894061233 21.312641991460623 70-71 20.903223715356013 28.890861981923937 28.93083675950899 21.275077543211065 72-73 21.049907102861663 28.796639495747556 28.666195509799753 21.487257891591028 74-75 20.960527976559696 28.928993969504223 28.559964364136057 21.55051368980002 76-77 20.955668038959836 28.915885124181646 28.616118543342644 21.51232829351588 78-79 20.955413105622974 28.9793450871716 28.560589430385246 21.504652376820175 80-81 21.12801673755243 28.787067686897753 28.560413331106897 21.524502244442917 82-83 21.151763547378682 28.840255489061168 28.43133547305013 21.57664549051002 84-85 21.21533568668588 28.621766350201643 28.503411182549833 21.659486780562645 86-87 20.907876107368114 28.620840202588386 28.652712563849082 21.818571126194417 88-89 21.05976792929839 28.554380643881284 28.57702001103976 21.80883141578057 90-91 20.99773803876273 28.58095572036541 28.53852553128588 21.88278070958598 92-93 21.080634056316256 28.619948278385255 28.54874020643466 21.750677458863834 94-95 21.108822631005665 28.446691672977174 28.574571548397426 21.869914147619735 96-97 21.182889428542666 28.4064362306848 28.53348131523829 21.877193025534243 98-99 20.896878383919738 28.539718163223714 28.427258463329053 22.136144989527498 100-101 20.9553583969057 28.56356402073996 28.432392295294022 22.04868528706031 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 856.0 1 501.5 2 185.0 3 202.0 4 141.0 5 131.0 6 204.5 7 396.5 8 624.5 9 707.5 10 601.0 11 423.0 12 382.0 13 434.0 14 481.5 15 548.5 16 612.5 17 677.0 18 729.0 19 753.5 20 847.5 21 986.5 22 1132.5 23 1317.5 24 1559.5 25 2117.5 26 3259.0 27 5259.0 28 8251.0 29 12507.0 30 18671.5 31 25334.0 32 31503.0 33 37006.0 34 41380.5 35 45319.0 36 48252.0 37 48531.5 38 45275.5 39 41279.5 40 38051.5 41 34939.0 42 32881.0 43 31481.0 44 29969.0 45 28672.5 46 28356.0 47 28055.0 48 28167.0 49 28462.0 50 27696.0 51 26773.0 52 26019.0 53 25410.5 54 24233.0 55 22507.5 56 20412.5 57 18269.5 58 16613.0 59 14988.0 60 12936.5 61 10653.0 62 8567.0 63 6674.5 64 4990.5 65 3704.5 66 2709.5 67 1959.5 68 1441.0 69 1014.5 70 753.5 71 544.5 72 385.5 73 279.5 74 208.5 75 154.0 76 111.0 77 65.0 78 28.0 79 19.5 80 11.5 81 6.0 82 6.0 83 4.0 84 2.0 85 2.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019250733934231243 2 0.0 3 0.0017679245449804203 4 0.018464989692017722 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.821803027669001E-5 12-13 4.9109015138345005E-5 14-15 0.0 16-17 8.348532573518652E-4 18-19 9.821803027669001E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.419811362451051E-4 28-29 0.004665356438142775 30-31 4.9109015138345005E-5 32-33 2.4554507569172503E-4 34-35 0.011442400527234386 36-37 0.004419811362451051 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.9287212110676004E-4 60-61 0.0026518868174706303 62-63 0.010018239088222382 64-65 0.0022590146963638704 66-67 0.005451100680356296 68-69 0.0035358490899608406 70-71 9.821803027669001E-5 72-73 0.008004769467550236 74-75 0.006629717043676576 76-77 0.0017188155298420752 78-79 7.366352270751751E-4 80-81 0.007169916210198371 82-83 0.009379821891423896 84-85 0.0020625786358104904 86-87 0.0019152515903954552 88-89 4.910901513834501E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 4.9109015138345005E-5 98-99 4.9109015138345005E-5 100-101 0.00117861636332028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1018143.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.66727501302026 #Duplication Level Percentage of deduplicated Percentage of total 1 59.27634674222778 27.069892284444634 2 19.980490984491915 18.249091533679284 3 8.615340187442829 11.803173290120316 4 4.181776267553185 7.638813074130908 5 2.3228503129891545 5.303912202867796 6 1.448895076609824 3.9700253957131135 7 0.9480242329350788 3.0305578355117677 8 0.6922943100346033 2.5292155717039475 9 0.47761933520499106 1.963041617910801 >10 1.9917045068933996 14.599041783614272 >50 0.04822759289689632 1.4591484106581363 >100 0.014268549687179614 0.9794819079230254 >500 0.0010809505165647651 0.32738247586632807 >1k 0.0010809505165647651 1.0772226158557012 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 3953 0.38825587368375564 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2203 0.21637432069954812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2170 0.21313312570041734 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1562 0.1534165632921898 RNA PCR Primer, Index 33 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1023 0.10047704497305389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 9.821803027669001E-5 10-11 0.0 0.0 0.0 0.0 9.821803027669001E-5 12-13 0.0 0.0 0.0 0.0 9.821803027669001E-5 14-15 0.0 0.0 0.0 0.0 9.821803027669001E-5 16-17 0.0 0.0 0.0 0.0 9.821803027669001E-5 18-19 0.0 0.0 0.0 0.0 9.821803027669001E-5 20-21 0.0 0.0 0.0 2.9465409083007E-4 9.821803027669001E-5 22-23 0.0 0.0 0.0 7.857442422135202E-4 1.9643606055338002E-4 24-25 0.0 0.0 0.0 0.00117861636332028 3.43763105968415E-4 26-27 0.0 0.0 0.0 0.0020625786358104904 3.9287212110676004E-4 28-29 0.0 0.0 0.0 0.0032903040142691154 3.9287212110676004E-4 30-31 0.0 0.0 0.0 0.005745754771186366 3.9287212110676004E-4 32-33 0.0 0.0 0.0 0.013210325072214806 3.9287212110676004E-4 34-35 0.0 0.0 0.0 0.028925209916485208 3.9287212110676004E-4 36-37 0.0 0.0 0.0 0.04640801930573603 3.9287212110676004E-4 38-39 0.0 0.0 0.0 0.061680923013761324 3.9287212110676004E-4 40-41 0.0 0.0 0.0 0.0786726422516287 3.9287212110676004E-4 42-43 0.0 0.0 0.0 0.10077169906388395 4.419811362451051E-4 44-45 0.0 0.0 0.0 0.13043354420744435 4.910901513834501E-4 46-47 0.0 0.0 0.0 0.16284549419875205 4.910901513834501E-4 48-49 0.0 0.0 0.0 0.1979584400226687 4.910901513834501E-4 50-51 0.0 0.0 0.0 0.2323347506195102 4.910901513834501E-4 52-53 0.0 0.0 0.0 0.2739300864416885 4.910901513834501E-4 54-55 0.0 0.0 0.0 0.32554366135208906 4.910901513834501E-4 56-57 0.0 0.0 0.0 0.384179825427273 4.910901513834501E-4 58-59 0.0 0.0 0.0 0.43800330601889914 4.910901513834501E-4 60-61 0.0 0.0 0.0 0.49541174471562444 4.910901513834501E-4 62-63 0.0 0.0 0.0 0.5645572380304142 4.910901513834501E-4 64-65 0.0 0.0 0.0 0.6567348594450878 4.910901513834501E-4 66-67 9.821803027669001E-5 0.0 0.0 0.7717481728990918 4.910901513834501E-4 68-69 9.821803027669001E-5 0.0 0.0 0.8867614863530958 4.910901513834501E-4 70-71 9.821803027669001E-5 0.0 0.0 0.9950959737482848 4.910901513834501E-4 72-73 9.821803027669001E-5 0.0 0.0 1.1130558281105896 4.910901513834501E-4 74-75 9.821803027669001E-5 0.0 0.0 1.2614141628435298 4.910901513834501E-4 76-77 9.821803027669001E-5 0.0 0.0 1.4192014284830323 4.910901513834501E-4 78-79 9.821803027669001E-5 0.0 0.0 1.5869578241956188 4.910901513834501E-4 80-81 9.821803027669001E-5 0.0 0.0 1.756924125589431 4.910901513834501E-4 82-83 9.821803027669001E-5 0.0 0.0 1.9441276912968022 4.910901513834501E-4 84-85 9.821803027669001E-5 0.0 0.0 2.152742787604492 4.910901513834501E-4 86-87 9.821803027669001E-5 0.0 0.0 2.3907250749649114 4.910901513834501E-4 88-89 9.821803027669001E-5 0.0 0.0 2.6762448889792494 4.910901513834501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1660 0.0 58.380077 1 GTATCAA 2970 0.0 39.82772 1 CGGAGAT 140 4.5474735E-10 33.932396 1 TCAACGC 3595 0.0 32.63768 4 TATCAAC 3625 0.0 32.62966 2 ATCAACG 3610 0.0 32.502068 3 CAACGCA 3700 0.0 31.70836 5 AACGCAG 3730 0.0 31.453335 6 GTGGTAT 835 0.0 31.29095 1 TGGTATC 835 0.0 30.720516 2 ACGCAGA 4185 0.0 28.033678 7 CGCAGAG 4290 0.0 27.1261 8 CGTCAGA 280 1.8189894E-12 23.75151 2 GCAGAGT 4715 0.0 23.673624 9 GAGTACT 2680 0.0 23.483328 12-13 CAGAGTA 4680 0.0 21.059633 10-11 GTACTTT 3130 0.0 19.651876 14-15 AGAGTAC 4230 0.0 19.313751 10-11 AGTACTT 3215 0.0 17.28556 12-13 TACTCCG 180 0.0026189396 15.832785 5 >>END_MODULE