FastQCFastQC Report
Fri 10 Feb 2017
SRR2031679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646906
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.5339261036379319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.5269699152581673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.2484132161395937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10320.15952858684260154No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.1443795543711142No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.10140576838056842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.064.468111
GTATCAA37600.038.7900732
GTGGTAT8400.037.8935781
TGGTATC9000.033.7837262
TATCAAC46150.031.498233
ATCAACG46450.031.3970664
TCAACGC46600.031.1940635
CAACGCA48050.030.2456996
AACGCAG49150.029.6654157
GCGTCAG1652.6375346E-928.7929481
CGTCAGA1703.6288839E-927.9460952
ACGCAGA55050.026.3175418
CGCAGAG56200.025.8635379
GAGTACT30350.024.18558912-13
CAGAGTA57500.022.80224810-11
GTACATG40900.021.8375851
TTGCGTG550.002878496221.58969562-63
TACATGG40800.021.4253392
GTACTTT33500.021.34496114-15
GCAGAGT59500.020.8365769
Produced by FastQC (version 0.11.5)