##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031679_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646906 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.626693831870472 33.0 31.0 34.0 30.0 34.0 2 31.93740976277852 33.0 31.0 34.0 30.0 34.0 3 32.01663456514548 34.0 31.0 34.0 30.0 34.0 4 35.46650054258269 37.0 35.0 37.0 35.0 37.0 5 35.471158097157854 37.0 35.0 37.0 35.0 37.0 6 35.51246858121582 37.0 36.0 37.0 35.0 37.0 7 35.493762617752814 37.0 36.0 37.0 35.0 37.0 8 35.49611535524481 37.0 36.0 37.0 35.0 37.0 9 37.22451329868636 39.0 38.0 39.0 35.0 39.0 10-11 37.15330898152127 39.0 38.0 39.0 35.0 39.0 12-13 37.09167947120602 39.0 38.0 39.0 34.0 39.0 14-15 38.372840876417904 41.0 38.0 41.0 34.0 41.0 16-17 38.292006875805754 40.0 38.0 41.0 34.0 41.0 18-19 38.36055006446068 40.5 38.5 41.0 34.0 41.0 20-21 38.388636061498886 41.0 39.0 41.0 34.0 41.0 22-23 38.27937211897864 40.5 38.5 41.0 34.0 41.0 24-25 38.18846710341224 40.5 38.0 41.0 34.0 41.0 26-27 38.06228571075241 40.0 38.0 41.0 34.0 41.0 28-29 37.93728223265822 40.0 38.0 41.0 34.0 41.0 30-31 37.720959459334125 40.0 38.0 41.0 33.0 41.0 32-33 37.45611186169243 40.0 37.5 41.0 33.0 41.0 34-35 37.25375015844651 40.0 37.0 41.0 32.5 41.0 36-37 37.07137281150584 40.0 37.0 41.0 31.5 41.0 38-39 36.86027722729423 40.0 36.5 41.0 31.0 41.0 40-41 36.717478273504966 40.0 36.5 41.0 31.0 41.0 42-43 36.618394326223594 40.0 37.0 41.0 30.0 41.0 44-45 36.37133061062967 40.0 36.5 41.0 30.0 41.0 46-47 36.12675102719715 40.0 36.5 41.0 29.0 41.0 48-49 36.00792696311365 40.0 37.0 41.0 27.5 41.0 50-51 35.42553168466516 39.5 36.0 40.5 26.0 41.0 52-53 35.43537082667343 39.5 36.0 40.5 26.0 41.0 54-55 35.64544230537358 40.0 36.0 41.0 26.0 41.0 56-57 35.516058747329595 40.0 36.0 41.0 26.0 41.0 58-59 35.32534479507069 40.0 35.0 41.0 25.0 41.0 60-61 35.09108046609553 39.0 35.0 41.0 24.0 41.0 62-63 34.806420871038455 39.0 35.0 41.0 23.0 41.0 64-65 34.544831861197764 39.0 35.0 41.0 22.5 41.0 66-67 34.25250268199707 38.0 35.0 40.5 22.0 41.0 68-69 33.905432937706564 37.0 34.5 40.0 21.0 41.0 70-71 33.53923444828151 37.0 34.0 39.5 20.0 41.0 72-73 33.16952231081486 36.0 34.0 39.0 18.5 41.0 74-75 32.764232361425 36.0 34.0 39.0 17.0 41.0 76-77 32.38656079863226 35.0 34.0 37.5 13.5 40.0 78-79 31.98998618037242 35.0 33.5 37.0 9.5 39.0 80-81 31.626519154251156 35.0 33.0 37.0 7.0 39.0 82-83 31.292144453753714 35.0 33.0 36.0 4.5 38.5 84-85 30.962681595162202 35.0 33.0 36.0 2.0 37.0 86-87 30.679262520366173 35.0 32.5 35.5 2.0 37.0 88-89 30.402574717192294 35.0 32.0 35.0 2.0 36.5 90-91 30.157345889510996 35.0 32.0 35.0 2.0 36.0 92-93 29.920226431660858 35.0 32.0 35.0 2.0 36.0 94-95 29.658513756248976 35.0 31.5 35.0 2.0 36.0 96-97 29.384623113713587 35.0 31.0 35.0 2.0 35.5 98-99 29.110978256500946 35.0 31.0 35.0 2.0 35.0 100-101 28.52213459142441 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08504250235703026 1101 2 0.1455567212771669 1101 3 0.2108880217071487 1101 4 0.5107710185563334 1101 5 0.2835271401033097 1101 6 0.36545590787129356 1101 7 0.3955124927267164 1101 8 0.29179990892390606 1101 9 0.29605255625814664 1101 10-11 0.20249732012182875 1101 12-13 0.35308222731850947 1101 14-15 0.2948789601414319 1101 16-17 0.22656169769636136 1101 18-19 0.29600444092769607 1101 20-21 0.24865942446953682 1101 22-23 0.3696705465028458 1101 24-25 0.12030662687834592 1101 26-27 -0.1250190231368009 1101 28-29 -0.06923478419185614 1101 30-31 0.12713744127153603 1101 32-33 0.18766692438628496 1101 34-35 0.11418111198705105 1101 36-37 -0.11950477069083831 1101 38-39 0.22769479107098078 1101 40-41 -0.028164110424057753 1101 42-43 0.268453711685531 1101 44-45 0.2965427755055714 1101 46-47 0.4161621851301476 1101 48-49 0.19029752906131137 1101 50-51 0.1643318133861129 1101 52-53 0.08824965796615913 1101 54-55 0.26407912077104356 1101 56-57 -0.0033878041124424385 1101 58-59 -0.22347135073656688 1101 60-61 -0.23363746109799877 1101 62-63 0.1418816419897908 1101 64-65 0.2443456402249211 1101 66-67 -0.12174911715412406 1101 68-69 -0.16939877930403213 1101 70-71 -0.15690695187215198 1101 72-73 0.273177250169212 1101 74-75 0.23563611019120145 1101 76-77 0.16417078974243537 1101 78-79 -0.005726656973713773 1101 80-81 0.0453404877246264 1101 82-83 0.3064865384260571 1101 84-85 0.07796684482796223 1101 86-87 0.03563845679132882 1101 88-89 0.009463062323384008 1101 90-91 -0.322653419145837 1101 92-93 -0.15823328040440643 1101 94-95 -0.04247347749601005 1101 96-97 -0.1146630742991448 1101 98-99 -0.1470712379872232 1101 100-101 -0.11730157848936074 1102 1 -0.3240624660593916 1102 2 -0.23230171792428322 1102 3 -0.10916642475383043 1102 4 -0.2082549947527994 1102 5 -0.22303668324817494 1102 6 -0.1500613335080132 1102 7 -0.04958732578508318 1102 8 -0.08094661255219648 1102 9 -0.0031912428949070204 1102 10-11 -0.037051984173388064 1102 12-13 -0.10178770613581634 1102 14-15 0.05401386637252159 1102 16-17 0.011725037079301615 1102 18-19 0.08221133747941423 1102 20-21 -0.12346701231207646 1102 22-23 0.09734749750103333 1102 24-25 -0.07273632532976393 1102 26-27 -0.13425979748647165 1102 28-29 0.04333327388437169 1102 30-31 -0.42125941293294744 1102 32-33 -0.199507909249526 1102 34-35 0.1756753648606093 1102 36-37 -3.426412292526493E-4 1102 38-39 0.002498178850778743 1102 40-41 0.08986371776710911 1102 42-43 0.2448904932947258 1102 44-45 0.18150950266830534 1102 46-47 -0.006554088312078932 1102 48-49 0.17844029978121512 1102 50-51 -0.11469117511963844 1102 52-53 -0.09638434082392422 1102 54-55 -0.11258452109223072 1102 56-57 -0.4373260981233287 1102 58-59 -0.6784622763264068 1102 60-61 -0.2870706129431255 1102 62-63 -0.22797619225098487 1102 64-65 -0.15606270823242596 1102 66-67 -0.21418710868467628 1102 68-69 -0.35601402431310447 1102 70-71 -0.34252401176325975 1102 72-73 0.049281303405756205 1102 74-75 -0.3049416272558645 1102 76-77 -0.07000948853946909 1102 78-79 -0.28160384995616994 1102 80-81 -0.008939609069077648 1102 82-83 0.06987129341698406 1102 84-85 0.030053959165531552 1102 86-87 -0.05970334599149041 1102 88-89 -0.16429676828762751 1102 90-91 -0.23268064237632657 1102 92-93 0.03030271475590851 1102 94-95 -0.27910384652202325 1102 96-97 -0.6887102612319964 1102 98-99 -0.6030452246780875 1102 100-101 -0.32664822095760115 1103 1 0.045816696923221656 1103 2 0.11694673477244777 1103 3 0.2827728620130401 1103 4 0.14999728936155066 1103 5 0.1882954711874305 1103 6 0.21759266000804445 1103 7 0.24040677972626412 1103 8 0.13404012484833316 1103 9 0.17068143615018272 1103 10-11 0.2394069017682554 1103 12-13 0.3133611297026775 1103 14-15 0.4977804446218599 1103 16-17 0.3429584588299619 1103 18-19 0.4353206802439331 1103 20-21 0.14652267233277882 1103 22-23 0.22194225140613355 1103 24-25 0.3148410398338086 1103 26-27 0.26679384236027914 1103 28-29 0.13791771243432294 1103 30-31 0.2987523760443622 1103 32-33 0.295201787184304 1103 34-35 0.1928135906195223 1103 36-37 0.27181326799561845 1103 38-39 0.18765880366657228 1103 40-41 0.36873197594571394 1103 42-43 0.4607614039932244 1103 44-45 0.19768987402109417 1103 46-47 0.4122935391562308 1103 48-49 0.21881754705501777 1103 50-51 0.2664685655228567 1103 52-53 0.1760589247227884 1103 54-55 0.14109216620514076 1103 56-57 0.2508407159055537 1103 58-59 0.2896190676170036 1103 60-61 0.4659576806024006 1103 62-63 0.40380246970008926 1103 64-65 0.37237083140801275 1103 66-67 0.25488156210813173 1103 68-69 0.22230162555427313 1103 70-71 0.17132066846073002 1103 72-73 0.17011832079449363 1103 74-75 0.25221280834684734 1103 76-77 0.32829584597276096 1103 78-79 0.3348491414968251 1103 80-81 0.15948803608270268 1103 82-83 0.06613566123307635 1103 84-85 -0.06593778855035382 1103 86-87 0.23957457914850622 1103 88-89 0.37840143389333747 1103 90-91 0.31200694972505616 1103 92-93 -0.27912846708906613 1103 94-95 -0.054641718611616596 1103 96-97 0.11752316051866885 1103 98-99 0.02951445701952693 1103 100-101 0.19999486775445163 1104 1 0.23955470494694353 1104 2 0.43452915235486245 1104 3 0.505553256222008 1104 4 0.44603775683059155 1104 5 0.4313968142887745 1104 6 0.5351837990277559 1104 7 0.4440087333282179 1104 8 0.39937241160919257 1104 9 0.3572591441564654 1104 10-11 0.6079573988901856 1104 12-13 0.5891635019336192 1104 14-15 0.7813361746061602 1104 16-17 0.6447768785055317 1104 18-19 0.6266166858256526 1104 20-21 0.6758703088232778 1104 22-23 0.6426207792275207 1104 24-25 0.738101113093883 1104 26-27 0.7095942958750143 1104 28-29 0.602399659059131 1104 30-31 0.8043035708812667 1104 32-33 0.8250639884750797 1104 34-35 0.4988749895380664 1104 36-37 0.6208103027069427 1104 38-39 0.735508969373889 1104 40-41 0.67578325799699 1104 42-43 0.6300183375358657 1104 44-45 0.5737626108081173 1104 46-47 0.6574618628959925 1104 48-49 0.43999319680209226 1104 50-51 0.41722296984869445 1104 52-53 0.3077090206585993 1104 54-55 0.5924178246736602 1104 56-57 0.6179870616233316 1104 58-59 0.4714087065495036 1104 60-61 0.6675798679388762 1104 62-63 0.6028823580656919 1104 64-65 0.7218519058890891 1104 66-67 0.6727997550263254 1104 68-69 0.8079374183329193 1104 70-71 0.6409090166205047 1104 72-73 0.5108517936708239 1104 74-75 0.6770252974450486 1104 76-77 0.6949799555140075 1104 78-79 0.6576818643295468 1104 80-81 0.5368907920568908 1104 82-83 0.4967479061310378 1104 84-85 0.5687473675633719 1104 86-87 0.5576846436871428 1104 88-89 0.7575833630752626 1104 90-91 0.5772302185197553 1104 92-93 0.5710721259686693 1104 94-95 0.7064345067503197 1104 96-97 0.47937559379967354 1104 98-99 0.3285463774800199 1104 100-101 0.4934843148153263 1105 1 -0.16930429319776863 1105 2 0.044377637203346865 1105 3 -0.20162052238034178 1105 4 -0.2070234676592051 1105 5 -0.062109679217719815 1105 6 0.059611752027137754 1105 7 0.17939834371782837 1105 8 0.03581335162155597 1105 9 0.08406804953679625 1105 10-11 0.1341246839860375 1105 12-13 0.0621845285260747 1105 14-15 0.25408663842805623 1105 16-17 0.022366550738055935 1105 18-19 0.028634311057565753 1105 20-21 -0.019901181591066575 1105 22-23 -0.1838325293686438 1105 24-25 -0.15394434145157732 1105 26-27 -0.032250169183733135 1105 28-29 -0.11934585732924319 1105 30-31 0.040736780528767724 1105 32-33 0.08785774234026178 1105 34-35 0.05828284358878477 1105 36-37 0.17387925756161593 1105 38-39 0.2253821913899614 1105 40-41 0.34650836622210335 1105 42-43 0.16948185021366413 1105 44-45 0.4398726912039095 1105 46-47 0.6143650787277792 1105 48-49 0.4813119970494668 1105 50-51 0.37710237615667097 1105 52-53 0.6182764294402929 1105 54-55 0.47050997062294897 1105 56-57 0.4504314029962373 1105 58-59 0.3670874325853717 1105 60-61 0.451979991624718 1105 62-63 0.5024649183625414 1105 64-65 0.5752068842440607 1105 66-67 0.6262629584895265 1105 68-69 0.4679559712086174 1105 70-71 0.5704853926254501 1105 72-73 0.30831623399240726 1105 74-75 0.5197896759237111 1105 76-77 0.5885328312097471 1105 78-79 0.5903117344594122 1105 80-81 0.6558611074557739 1105 82-83 0.712347782445196 1105 84-85 0.9746688270562593 1105 86-87 0.8231840252579516 1105 88-89 0.7042824972743986 1105 90-91 0.6374884127065208 1105 92-93 0.6648414518808501 1105 94-95 0.7683547293848285 1105 96-97 0.7686844991800079 1105 98-99 0.532898572903644 1105 100-101 0.7972490025085861 1106 1 0.07520385822527587 1106 2 0.27035644562896266 1106 3 0.25051245102922337 1106 4 0.11773687837774105 1106 5 0.22950296345874932 1106 6 0.26578324011352095 1106 7 0.24404380463988673 1106 8 0.3857586191200184 1106 9 0.2636437929423252 1106 10-11 0.1785231047142446 1106 12-13 0.21596160251591812 1106 14-15 0.4028540943763659 1106 16-17 0.33624814786433177 1106 18-19 0.2842204802075585 1106 20-21 0.3083702809888962 1106 22-23 0.27477003827647195 1106 24-25 0.41129494054302995 1106 26-27 0.27526811040900867 1106 28-29 0.37386970370546635 1106 30-31 0.4222475569863562 1106 32-33 0.5036214998593422 1106 34-35 0.36660882611688805 1106 36-37 0.5530280343167746 1106 38-39 0.5335580580764727 1106 40-41 0.30173797703681515 1106 42-43 0.4711814803707455 1106 44-45 0.4780499394875477 1106 46-47 0.7675217624695634 1106 48-49 0.9451314223050602 1106 50-51 0.735499298383381 1106 52-53 1.031850886897736 1106 54-55 1.1129234082491593 1106 56-57 0.8806461350726735 1106 58-59 0.848920758833593 1106 60-61 0.7190400592166952 1106 62-63 0.7578850301656317 1106 64-65 0.8903013642321653 1106 66-67 0.7503170958415453 1106 68-69 0.7213196288275867 1106 70-71 0.7733755875369255 1106 72-73 0.6971959712764004 1106 74-75 0.48981964595368055 1106 76-77 0.8074920634668459 1106 78-79 0.8140999143646184 1106 80-81 0.7602699964391313 1106 82-83 0.6246190218440972 1106 84-85 0.7191504093038041 1106 86-87 0.5774178233747271 1106 88-89 0.4102799572612206 1106 90-91 0.4717087136821405 1106 92-93 0.5448577122162597 1106 94-95 0.6864021075385907 1106 96-97 0.9023022112551686 1106 98-99 1.0453900707674784 1106 100-101 1.075535875210356 1107 1 0.025303876410401216 1107 2 0.262245880071589 1107 3 0.3767899560301373 1107 4 0.39615113551539594 1107 5 0.3643638472558024 1107 6 0.35605419846958597 1107 7 0.38228712160660194 1107 8 0.3049802957885035 1107 9 0.44760451307325866 1107 10-11 0.5000906624520169 1107 12-13 0.327036343377884 1107 14-15 0.3695753164167357 1107 16-17 0.28398409985560846 1107 18-19 0.36352580844906157 1107 20-21 0.22430045011056166 1107 22-23 0.1621131915770775 1107 24-25 0.39860172359449564 1107 26-27 0.40183657740301015 1107 28-29 0.5994561739727899 1107 30-31 0.45601733330932603 1107 32-33 0.32255221453473126 1107 34-35 0.38597598378191833 1107 36-37 0.4624115585939066 1107 38-39 0.41415453016229975 1107 40-41 0.15078325799699854 1107 42-43 0.4094793527111733 1107 44-45 0.43358730991852923 1107 46-47 0.28750721436991 1107 48-49 0.3000141282515969 1107 50-51 0.05450275355704548 1107 52-53 0.2179392666031319 1107 54-55 0.5470750721880506 1107 56-57 0.38075524582008313 1107 58-59 0.3144053924033372 1107 60-61 0.09159870624343114 1107 62-63 0.3832896491872688 1107 64-65 0.1723708314080099 1107 66-67 0.06855677578334252 1107 68-69 0.12059222384486645 1107 70-71 0.20123519837525805 1107 72-73 0.24533199600816857 1107 74-75 0.14366579979984095 1107 76-77 0.11547533315224712 1107 78-79 0.2100628167104972 1107 80-81 0.3167529933476594 1107 82-83 0.33109292619034036 1107 84-85 0.41098528837272497 1107 86-87 0.5002583398322642 1107 88-89 0.4946407501326533 1107 90-91 0.5444855822036914 1107 92-93 0.6644612765006777 1107 94-95 0.5992044352345332 1107 96-97 0.4927368357323445 1107 98-99 0.5790871065921763 1107 100-101 0.47520854296812587 1108 1 -0.13809153239714078 1108 2 -0.09153912876785242 1108 3 -0.004058364476037468 1108 4 -0.009067457883595864 1108 5 0.006713410600703185 1108 6 0.03310754084174761 1108 7 -0.019499375980828404 1108 8 -0.10292601942730073 1108 9 -0.07968344003117522 1108 10-11 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-0.5233497000999492 2316 100-101 -0.7408211094820487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7407.0 3 832.0 4 646.0 5 602.0 6 659.0 7 798.0 8 936.0 9 1107.0 10 1201.0 11 1519.0 12 1996.0 13 2523.0 14 3021.0 15 3897.0 16 4968.0 17 7295.0 18 6589.0 19 3494.0 20 2879.0 21 2766.0 22 2918.0 23 3054.0 24 3272.0 25 3594.0 26 4317.0 27 4988.0 28 5831.0 29 7048.0 30 8662.0 31 10598.0 32 13302.0 33 17867.0 34 25059.0 35 38937.0 36 65162.0 37 128057.0 38 186611.0 39 62448.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40089060651666 20.95712330379096 15.313725125880232 21.32826096381214 2 16.429965005729148 22.653835434021865 39.393639094484534 21.522560465764453 3 18.277222449308216 27.902158968451563 32.05798744008027 21.76263114215996 4 13.485571302709223 20.395738203957382 37.774433249565284 28.344257243768105 5 13.155262848260785 37.85693053671949 34.5251144097448 14.462692205274925 6 27.000575794348546 37.86126451868546 19.793704648455883 15.344455038510116 7 23.859838675473316 35.333662524940905 23.817889964351334 16.98860883523444 8 24.08894914582695 34.11770532500054 22.18389096795961 19.609454561212896 9 26.032968954827485 17.33524421820613 22.590745105808026 34.04104172115836 10-11 23.171630964217595 27.859439258121547 28.877471670812717 20.09145810684814 12-13 24.861124224361962 26.970766823354964 28.450999178121627 19.717109774161447 14-15 22.673982977116015 28.26455134576146 27.660328754059886 21.401136923062634 16-17 20.08021620131929 30.728719521211 28.692039304545197 20.499024972924516 18-19 20.052577635616547 28.716508077076707 31.57057881845853 19.660335468848213 20-21 22.115017690682805 27.362234436572457 31.236536542812537 19.2862113299322 22-23 21.86252678946672 27.616312144016458 30.772380282333312 19.748780784183513 24-25 20.948515357173257 28.061800143937226 31.232288369717466 19.75739612917205 26-27 20.39581417953647 28.313645551830565 31.569094516829026 19.72144575180394 28-29 20.264376845355798 28.115347555713782 31.83063164860169 19.78964395032873 30-31 20.832143528739874 27.91564033928971 31.41517486451705 19.83704126745337 32-33 20.537134826939386 28.118248283632447 31.083277101273428 20.261339788154743 34-35 20.762237643165292 28.343129226849367 31.042817626106974 19.85181550387837 36-37 20.786393797518418 28.22316425941506 31.065968317312937 19.92447362575358 38-39 20.33964676365111 28.43481410283199 31.202647337880734 20.022891795636163 40-41 20.52809821800323 28.335110738260227 31.207618953276178 19.929172090460366 42-43 20.74209584656625 28.288109255923082 30.676147936283886 20.293646961226777 44-45 20.842153658251526 28.514230296200378 30.264466558358883 20.37914948718921 46-47 20.970398428514596 28.69036691212096 30.006671692365323 20.332562966999117 48-49 20.760191139257163 29.019075995117017 29.827075171481145 20.393657694144675 50-51 20.86609907120743 28.98660990712074 29.358049535603715 20.78924148606811 52-53 21.000603612387984 29.15602606367337 29.13575087833341 20.70761944560524 54-55 21.19355495182773 29.110737800626925 28.988865941729003 20.706841305816344 56-57 21.03924649211742 29.265376250880355 28.907274261080886 20.78810299592134 58-59 20.834794021663846 29.219261119997768 29.255014127081047 20.690930731257335 60-61 20.993732486521246 29.254483953571338 29.202325611989917 20.549457947917496 62-63 20.93118098171378 29.14434736850251 29.286349953181713 20.638121696602 64-65 20.987021712527103 28.95248725353677 29.20822925753545 20.85226177640068 66-67 20.83044061431677 29.254193184870093 29.051876997330574 20.863489203482562 68-69 20.816166357190237 29.2797726257714 29.052522239345123 20.85153877769324 70-71 20.912623448023943 28.953374355624728 29.238249946019906 20.895752250331427 72-73 20.918976606897278 29.00854633385263 29.001658498002914 21.07081856124718 74-75 20.822422993116426 29.40251572327044 28.759811568365272 21.015249715247858 76-77 20.66673426217822 29.383846272326835 28.842282385667485 21.107137079827464 78-79 20.847403972002944 29.2894775745689 28.77786489555284 21.085253557875312 80-81 20.72731438266359 29.322686159137945 28.68777215852184 21.262227299676624 82-83 20.75175806270585 29.481811743123526 28.667742597172392 21.098687596998232 84-85 20.786721215808107 29.393535524277 28.612603576033667 21.207139683881223 86-87 20.554207448517147 29.264939799602924 28.803306667905122 21.377546083974813 88-89 20.679962324926613 29.257078200543145 28.690713806428448 21.372245668101797 90-91 20.528744902295117 29.343585115514436 28.801403711910883 21.32626627027956 92-93 20.623081657061242 29.213232647342284 28.76958983747547 21.394095858121002 94-95 20.590661785761274 29.152394146009243 28.75552391050298 21.501420157726507 96-97 20.576963105281607 29.10249753743753 28.82602508757282 21.494514269708045 98-99 20.524505422649376 29.208250629708303 28.696668253028594 21.57057569461372 100-101 20.431038220229283 29.276162988746368 28.744216798072575 21.54858199295177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1847.0 1 1075.0 2 307.5 3 284.0 4 232.5 5 246.5 6 350.0 7 587.5 8 943.5 9 1106.5 10 954.0 11 752.0 12 711.5 13 773.5 14 858.5 15 914.5 16 960.0 17 1032.0 18 1115.0 19 1231.5 20 1365.5 21 1534.0 22 1726.5 23 1983.0 24 2245.5 25 2722.0 26 3729.5 27 5294.5 28 7150.5 29 9438.0 30 12246.5 31 14630.5 32 17242.0 33 20244.5 34 22508.0 35 23837.5 36 25186.5 37 25920.0 38 24660.5 39 23535.5 40 22837.5 41 22015.0 42 21596.5 43 21981.0 44 22524.5 45 22506.5 46 22559.0 47 22155.5 48 21797.0 49 21591.5 50 20535.5 51 18982.5 52 17490.0 53 16080.0 54 14216.0 55 12440.5 56 10983.5 57 9362.0 58 8309.5 59 7584.5 60 6514.0 61 5415.5 62 4202.5 63 2864.0 64 1905.5 65 1390.0 66 1057.5 67 734.0 68 551.5 69 446.0 70 344.0 71 267.0 72 230.0 73 209.5 74 163.0 75 120.5 76 95.0 77 62.0 78 43.5 79 38.0 80 30.5 81 28.0 82 22.5 83 18.5 84 13.0 85 7.0 86 5.0 87 3.0 88 3.0 89 4.0 90 2.5 91 1.0 92 0.5 93 0.5 94 2.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16292939005048646 2 0.16787601289831908 3 0.16107440648254925 4 0.16617561129437664 5 0.1517994886428631 6 0.13015801368359545 7 0.1358775463514019 8 0.12119225977189885 9 0.12103767780790409 10-11 0.1095213214902938 12-13 0.1275301202956844 14-15 0.14716202972302003 16-17 0.15867838604063034 18-19 0.15520029185074802 20-21 0.14600266499305928 22-23 0.13858273072131036 24-25 0.12227433351986225 26-27 0.12675721047571054 28-29 0.13270861608950915 30-31 0.12235162450185962 32-33 0.11964644013195117 34-35 0.13711420206336006 36-37 0.14012855036125804 38-39 0.12722095636769484 40-41 0.12876677600764252 42-43 0.12211975155586748 44-45 0.14028313232525283 46-47 0.13804169384732867 48-49 0.1529588533728239 50-51 0.14005125937926066 52-53 0.12304724333983609 54-55 0.11369503451815256 56-57 0.13309507099949605 58-59 0.12467035396178117 60-61 0.12312453432183346 62-63 0.12227433351986225 64-65 0.14569350106506973 66-67 0.13719149304535744 68-69 0.14260186178517437 70-71 0.1289986489536347 72-73 0.12977155877360855 74-75 0.11284483371618133 76-77 0.14012855036125804 78-79 0.14059229625324235 80-81 0.14113333312722404 82-83 0.1473939026690122 84-85 0.136495874207381 86-87 0.14507517320909064 88-89 0.13224487019752482 90-91 0.13981938643326852 92-93 0.15512300086875064 94-95 0.13154925135954837 96-97 0.11276754273418395 98-99 0.11956914914995377 100-101 0.16540270147440278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 646906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.88233620366295 #Duplication Level Percentage of deduplicated Percentage of total 1 67.84001415009313 37.91058478796756 2 18.07655875345826 20.20320667332042 3 6.9297541498638955 11.617525536342683 4 2.951706255658703 6.59792965412699 5 1.4621652598873507 4.085460531917057 6 0.8030555471694792 2.6925972046284814 7 0.48631095872276814 1.9023334746409972 8 0.3025023690063384 1.3523631269773375 9 0.21962057370574412 1.104561966335916 >10 0.8760282342618988 8.327229023634853 >50 0.03810042778168365 1.424503096802331 >100 0.012514694462499606 1.0578839830846252 >500 5.562086427578651E-4 0.24710379507312577 >1k 0.0011124172855157303 1.4767171451476986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3454 0.5339261036379319 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3409 0.5269699152581673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.2484132161395937 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1032 0.15952858684260154 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.1443795543711142 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 656 0.10140576838056842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5458196399476895E-4 0.0 12-13 0.0 0.0 0.0 3.8645490998692235E-4 0.0 14-15 0.0 0.0 0.0 4.6374589198430684E-4 0.0 16-17 0.0 0.0 0.0 4.6374589198430684E-4 0.0 18-19 0.0 0.0 0.0 4.6374589198430684E-4 0.0 20-21 0.0 0.0 0.0 6.956188379764602E-4 0.0 22-23 0.0 0.0 0.0 0.0010820737479633827 0.0 24-25 0.0 0.0 0.0 0.0017776925859398429 0.0 26-27 0.0 0.0 0.0 0.0026278933879110723 0.0 28-29 0.0 0.0 0.0 0.006724315433772449 0.0 30-31 0.0 0.0 0.0 0.013062175957557976 0.0 32-33 0.0 0.0 0.0 0.02558331504113426 0.0 34-35 0.0 0.0 0.0 0.04761124491038884 0.0 36-37 0.0 0.0 0.0 0.07257623209554402 0.0 38-39 0.0 0.0 0.0 0.09483603491079075 0.0 40-41 0.0 0.0 0.0 0.11408148942813948 0.0 42-43 0.0 0.0 0.0 0.13951022250527897 0.0 44-45 0.0 0.0 0.0 0.17405929145810983 0.0 46-47 0.0 0.0 0.0 0.2085310694289433 0.0 48-49 0.0 0.0 0.0 0.24903154399557276 0.0 50-51 0.0 0.0 0.0 0.28559017848033563 0.0 52-53 0.0 0.0 0.0 0.3298006201828395 0.0 54-55 0.0 0.0 0.0 0.3848317993649773 0.0 56-57 0.0 0.0 0.0 0.4465872939808875 0.0 58-59 0.0 0.0 0.0 0.4976766330811586 0.0 60-61 0.0 0.0 0.0 0.5530942671732832 0.0 62-63 0.0 0.0 0.0 0.62559320828683 0.0 64-65 0.0 0.0 0.0 0.7242937922974899 0.0 66-67 0.0 0.0 0.0 0.8457179250153809 0.0 68-69 0.0 0.0 0.0 0.9770353034289371 0.0 70-71 0.0 0.0 0.0 1.0952905058849354 0.0 72-73 0.0 0.0 0.0 1.2283855768844316 0.0 74-75 0.0 0.0 0.0 1.3859818891770983 0.0 76-77 0.0 0.0 0.0 1.562591783041122 0.0 78-79 0.0 0.0 0.0 1.7596837871344522 0.0 80-81 0.0 0.0 0.0 1.9450275619641801 0.0 82-83 0.0 0.0 0.0 2.1352406686597436 0.0 84-85 0.0 0.0 0.0 2.3538195657483465 0.0 86-87 0.0 0.0 0.0 2.619468670873357 0.0 88-89 0.0 0.0 0.0 2.948341799272228 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2255 0.0 64.46811 1 GTATCAA 3760 0.0 38.790073 2 GTGGTAT 840 0.0 37.893578 1 TGGTATC 900 0.0 33.783726 2 TATCAAC 4615 0.0 31.49823 3 ATCAACG 4645 0.0 31.397066 4 TCAACGC 4660 0.0 31.194063 5 CAACGCA 4805 0.0 30.245699 6 AACGCAG 4915 0.0 29.665415 7 GCGTCAG 165 2.6375346E-9 28.792948 1 CGTCAGA 170 3.6288839E-9 27.946095 2 ACGCAGA 5505 0.0 26.317541 8 CGCAGAG 5620 0.0 25.863537 9 GAGTACT 3035 0.0 24.185589 12-13 CAGAGTA 5750 0.0 22.802248 10-11 GTACATG 4090 0.0 21.837585 1 TTGCGTG 55 0.0028784962 21.589695 62-63 TACATGG 4080 0.0 21.425339 2 GTACTTT 3350 0.0 21.344961 14-15 GCAGAGT 5950 0.0 20.836576 9 >>END_MODULE