FastQCFastQC Report
Fri 10 Feb 2017
SRR2031679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646906
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.6515629782379512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.6498625766340087No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT34300.5302161365020575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.31843884582922405No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT14160.21888806101659283RNA PCR Primer, Index 33 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.18426170108176457No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT9740.15056283293090497RNA PCR Primer, Index 33 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9330.14422497240711943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.12119225977189885No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG6530.10094202248858414RNA PCR Primer, Index 33 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.063.0684661
GTGGTAT7950.040.636471
GTATCAA40900.040.5393031
TGGTATC8700.038.2195742
CGGAGAT756.908789E-638.0070531
GCGGTAT550.002576449734.5518651
ACCGTGC550.0025803634.5411838
TATCAAC50800.032.6338842
TCAACGC51050.032.381024
ATCAACG51300.032.223223
CAACGCA52000.031.7845295
AACGCAG54000.030.783236
CGTCAGA1004.9487622E-528.5008812
CATTCCG700.00839396727.1395029
TGCTCCG700.00839396727.1395025
ACGCAGA61950.026.986177
CGCAGAG61650.026.8863728
GCAGAGT65350.024.9280579
GAGTACT36150.024.83095412-13
GGTCGGG1151.279294E-424.7872071
Produced by FastQC (version 0.11.5)