##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031679_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646906 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75299811719168 34.0 31.0 34.0 31.0 34.0 2 32.91323468942937 34.0 31.0 34.0 31.0 34.0 3 32.94474467697007 34.0 31.0 34.0 31.0 34.0 4 36.356203219633144 37.0 37.0 37.0 35.0 37.0 5 36.33797491443888 37.0 37.0 37.0 35.0 37.0 6 36.38081575994039 37.0 37.0 37.0 35.0 37.0 7 36.337710579280454 37.0 37.0 37.0 35.0 37.0 8 36.33439788779204 37.0 37.0 37.0 35.0 37.0 9 38.16880041304301 39.0 39.0 39.0 37.0 39.0 10-11 38.14808101949897 39.0 39.0 39.0 37.0 39.0 12-13 38.09431432078231 39.0 39.0 39.0 37.0 39.0 14-15 39.52698846509385 41.0 39.0 41.0 36.5 41.0 16-17 39.490542675442796 41.0 39.0 41.0 36.5 41.0 18-19 39.557699263880686 41.0 39.5 41.0 37.0 41.0 20-21 39.58940943506475 41.0 40.0 41.0 37.0 41.0 22-23 39.491577601691745 41.0 39.0 41.0 36.0 41.0 24-25 39.420499268827314 41.0 39.0 41.0 36.0 41.0 26-27 39.28379548187836 41.0 39.0 41.0 36.0 41.0 28-29 39.160844079356195 41.0 39.0 41.0 35.5 41.0 30-31 38.945143498437176 40.0 38.5 41.0 35.0 41.0 32-33 38.664275026047065 40.0 38.0 41.0 35.0 41.0 34-35 38.487607163946535 40.0 38.0 41.0 35.0 41.0 36-37 38.366557119581515 40.0 38.0 41.0 35.0 41.0 38-39 38.370101684015914 40.0 38.0 41.0 35.0 41.0 40-41 38.254050047456666 40.0 38.0 41.0 34.5 41.0 42-43 38.09338296444923 40.0 38.0 41.0 34.0 41.0 44-45 37.794140879818706 40.0 38.0 41.0 33.5 41.0 46-47 37.40834989936714 40.0 38.0 41.0 33.0 41.0 48-49 37.026882576448514 40.0 38.0 41.0 32.0 41.0 50-51 36.7853490306165 40.0 37.0 41.0 31.0 41.0 52-53 36.81907804225034 40.0 37.0 41.0 31.0 41.0 54-55 36.7440215425425 40.0 37.0 41.0 31.0 41.0 56-57 36.5566743854594 40.0 37.0 41.0 31.0 41.0 58-59 36.36687246678807 40.0 36.5 41.0 31.0 41.0 60-61 36.10294617765177 40.0 36.0 41.0 30.0 41.0 62-63 35.81935706269535 39.0 35.5 41.0 29.0 41.0 64-65 35.463381078549276 39.0 35.0 41.0 28.0 41.0 66-67 35.06577153404049 39.0 35.0 41.0 27.5 41.0 68-69 34.61846620683685 38.0 35.0 40.0 26.0 41.0 70-71 34.25530216136502 37.0 35.0 40.0 26.0 41.0 72-73 33.871599583247026 37.0 35.0 39.0 25.5 41.0 74-75 33.47843349729327 36.0 34.0 39.0 25.0 41.0 76-77 32.56011924452703 35.0 33.5 38.0 22.5 39.0 78-79 32.7096865696098 35.0 34.0 37.0 23.0 39.0 80-81 32.45624093763236 35.0 34.0 37.0 23.0 39.0 82-83 32.15693083693767 35.0 34.0 36.5 21.5 39.0 84-85 31.838162731525138 35.0 34.0 36.0 20.0 37.0 86-87 31.563162963398085 35.0 34.0 36.0 19.5 37.0 88-89 31.31407654280529 35.0 34.0 35.5 18.0 37.0 90-91 31.092766182412902 35.0 33.0 35.0 13.5 36.0 92-93 30.915340868688805 35.0 33.0 35.0 7.0 36.0 94-95 30.72352629284626 35.0 33.0 35.0 2.0 36.0 96-97 30.55082423103202 35.0 33.0 35.0 2.0 36.0 98-99 30.34089883228784 35.0 33.0 35.0 2.0 35.0 100-101 29.69292292852439 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18980010001622816 1101 2 0.1767724264239945 1101 3 0.19503769493719147 1101 4 0.2004370837658982 1101 5 0.11319168689529846 1101 6 0.1820516461098265 1101 7 0.20813174277085977 1101 8 0.23732404994928658 1101 9 0.18408991990892076 1101 10-11 0.1886175035923543 1101 12-13 0.2640277829182267 1101 14-15 0.1217803694568147 1101 16-17 0.27521292569542055 1101 18-19 0.19431191801049152 1101 20-21 0.2302588864878956 1101 22-23 0.030091074721461553 1101 24-25 0.1435607423717542 1101 26-27 0.14394117176054522 1101 28-29 0.06473346725272933 1101 30-31 0.32423535455286867 1101 32-33 0.5479324904622302 1101 34-35 0.26784248374764985 1101 36-37 0.4136988888886606 1101 38-39 0.44380187935382054 1101 40-41 0.36327681153426283 1101 42-43 0.2766789446850666 1101 44-45 0.2774096800137187 1101 46-47 0.3858487484561621 1101 48-49 0.5721131136365614 1101 50-51 0.6460734187436188 1101 52-53 0.5556629276030804 1101 54-55 0.5862150142454325 1101 56-57 0.6906291026110907 1101 58-59 0.6414083883912767 1101 60-61 0.5977290238648223 1101 62-63 0.7301594738331048 1101 64-65 0.647764366860379 1101 66-67 0.517307926486076 1101 68-69 0.5250901966726076 1101 70-71 0.5844862949625309 1101 72-73 0.5790346516504101 1101 74-75 0.601658595786752 1101 76-77 0.39426075320347564 1101 78-79 0.3866011294658165 1101 80-81 0.3316282339496084 1101 82-83 0.384137081324603 1101 84-85 0.40861979448394337 1101 86-87 0.3712972136484254 1101 88-89 0.6348786247623757 1101 90-91 0.5627622441473825 1101 92-93 0.5704251624738355 1101 94-95 0.4752331114100663 1101 96-97 0.6951584629607375 1101 98-99 0.6134102815662636 1101 100-101 0.7023741519550235 1102 1 0.13499066262965442 1102 2 0.15568954681116765 1102 3 0.13859486372121665 1102 4 0.031713550795544165 1102 5 0.08085653867993159 1102 6 0.12721196068937957 1102 7 0.09660724186341696 1102 8 0.11838878074178893 1102 9 -0.0033571460317958213 1102 10-11 0.1600179875608987 1102 12-13 0.18760612532596355 1102 14-15 0.09682574150762946 1102 16-17 -0.05430915536931735 1102 18-19 0.06587876616536192 1102 20-21 0.1344482391799744 1102 22-23 0.01628284725624951 1102 24-25 0.014386513696628356 1102 26-27 0.15758303503942983 1102 28-29 0.2506076354317983 1102 30-31 0.18194860319170658 1102 32-33 0.15286291998431523 1102 34-35 0.047712417201978496 1102 36-37 0.11413748537989932 1102 38-39 0.03309407536107756 1102 40-41 0.05334033240539782 1102 42-43 0.15192092895609477 1102 44-45 -0.005848033234919114 1102 46-47 0.1547083975789647 1102 48-49 0.00810827395114444 1102 50-51 0.19723796804790794 1102 52-53 0.1633913002588585 1102 54-55 0.3369258430415556 1102 56-57 0.2636478563920974 1102 58-59 0.13824746885105554 1102 60-61 0.07846102628466411 1102 62-63 0.04197133106238482 1102 64-65 -0.20810375171190998 1102 66-67 -0.1185057456252423 1102 68-69 -0.17052383841511443 1102 70-71 -0.28089483026433015 1102 72-73 -0.15866952257826483 1102 74-75 -0.3359094622894716 1102 76-77 -0.30071807317280985 1102 78-79 -0.25393123593043754 1102 80-81 -0.3436288743383571 1102 82-83 -0.21902383821562665 1102 84-85 -0.02044856607261991 1102 86-87 -0.16183950746470188 1102 88-89 -0.2648037708819011 1102 90-91 -0.2837592319566724 1102 92-93 -0.32304126220856233 1102 94-95 -0.2433391813909047 1102 96-97 -0.48952090788015923 1102 98-99 -1.053468121337545 1102 100-101 -0.9644437548810316 1103 1 0.26726298800543447 1103 2 0.12063207554680133 1103 3 0.1854560485134371 1103 4 0.11532687233989947 1103 5 0.21211206476304056 1103 6 0.1449396353136052 1103 7 0.16620641663500635 1103 8 0.23395023078599309 1103 9 0.18139086457828313 1103 10-11 0.2580507019729197 1103 12-13 0.20208446308016192 1103 14-15 0.3252666492588787 1103 16-17 0.14840230941111798 1103 18-19 0.1917703075741457 1103 20-21 0.28862595801285806 1103 22-23 0.18947029199541277 1103 24-25 0.1335067612651457 1103 26-27 0.2749128316795719 1103 28-29 0.3202215464250173 1103 30-31 0.20536445936618009 1103 32-33 0.22834184718738015 1103 34-35 0.19559776939767204 1103 36-37 0.21819731443972046 1103 38-39 0.10009967512529983 1103 40-41 0.2895251246246744 1103 42-43 0.2372952289855661 1103 44-45 0.512564875695233 1103 46-47 0.5134690813396432 1103 48-49 0.5098324118821793 1103 50-51 0.34481385958932265 1103 52-53 0.15946409719372667 1103 54-55 0.43214393912172966 1103 56-57 0.32358006977258924 1103 58-59 0.39311779010068193 1103 60-61 0.17804841207894384 1103 62-63 0.0323412102253684 1103 64-65 0.30014853240243866 1103 66-67 0.2144739183889044 1103 68-69 0.08118556329429083 1103 70-71 -0.01744802802443246 1103 72-73 -0.09541429121958345 1103 74-75 0.16886507351954805 1103 76-77 0.22779651568660597 1103 78-79 0.1908521416115576 1103 80-81 0.06757065410254626 1103 82-83 0.08413708132460584 1103 84-85 0.0401762497246132 1103 86-87 -0.06943602971639606 1103 88-89 0.15885523294951653 1103 90-91 0.12904654464220755 1103 92-93 0.06603919756155463 1103 94-95 -0.02332739241359505 1103 96-97 -0.04103137059751916 1103 98-99 0.3041210328033337 1103 100-101 0.10300393153217158 1104 1 0.22615885404415081 1104 2 0.06076812996856518 1104 3 0.06727185175780903 1104 4 0.1161728517573053 1104 5 0.1360524101319598 1104 6 0.14896893934291455 1104 7 0.20943858129574267 1104 8 0.22581312979175294 1104 9 0.19651383684411172 1104 10-11 0.16658026764534384 1104 12-13 0.1604832071931952 1104 14-15 0.21607861507085602 1104 16-17 0.16616351645803462 1104 18-19 0.29059727425826054 1104 20-21 0.34177875759423415 1104 22-23 0.16543837153492547 1104 24-25 0.22747588737713187 1104 26-27 0.3841488337727128 1104 28-29 0.42978896313528736 1104 30-31 0.26310037424495647 1104 32-33 0.21757087213069326 1104 34-35 0.19264556287404133 1104 36-37 0.30427353980165606 1104 38-39 0.4358444858701134 1104 40-41 0.4786276887272436 1104 42-43 0.7029589303635575 1104 44-45 0.7173398626130734 1104 46-47 0.8843002343136632 1104 48-49 0.9234378540590455 1104 50-51 1.2010322443791424 1104 52-53 1.1897928953796608 1104 54-55 0.9691097510875437 1104 56-57 1.027077366127017 1104 58-59 1.278313150296043 1104 60-61 1.2851171370048178 1104 62-63 1.3303832475531223 1104 64-65 1.4208488638170493 1104 66-67 1.1581421549142874 1104 68-69 1.2386598350542712 1104 70-71 1.079455861736605 1104 72-73 1.305797988564123 1104 74-75 1.306214628726245 1104 76-77 1.2487541295013642 1104 78-79 1.3719484262792747 1104 80-81 1.461613912431524 1104 82-83 1.2048853806443276 1104 84-85 1.3311582835637843 1104 86-87 1.3782789537128721 1104 88-89 1.278469745421173 1104 90-91 1.254992820588484 1104 92-93 1.336957564908495 1104 94-95 1.39787006092671 1104 96-97 1.369299171875884 1104 98-99 1.2995989675669826 1104 100-101 1.4599287527427087 1105 1 0.14591766666011097 1105 2 0.16597839589312002 1105 3 0.08953530259269371 1105 4 0.12421243122545889 1105 5 0.17184399949497475 1105 6 0.13381742419139897 1105 7 0.18191848234707209 1105 8 0.22024726708303177 1105 9 0.028069186256608702 1105 10-11 0.07254037896259291 1105 12-13 0.2330326815283854 1105 14-15 0.24259376658599763 1105 16-17 0.1577374187462297 1105 18-19 0.19876053956438255 1105 20-21 0.1500270569139559 1105 22-23 0.2146034921286173 1105 24-25 0.15616327142165431 1105 26-27 0.10643547570221301 1105 28-29 0.15420145540492314 1105 30-31 0.019980398982120562 1105 32-33 -0.12194675310121283 1105 34-35 0.020915507215413243 1105 36-37 0.12485177109417833 1105 38-39 -0.05874425871863309 1105 40-41 -0.15475975716020685 1105 42-43 -0.29970804551771124 1105 44-45 -0.09017405204369311 1105 46-47 -0.19594095307038373 1105 48-49 -0.5252325231827584 1105 50-51 -0.5133307735553103 1105 52-53 -0.4147602895306619 1105 54-55 -0.6074595129817268 1105 56-57 -0.5763935677010537 1105 58-59 -0.711405464422576 1105 60-61 -0.8191654851349526 1105 62-63 -0.7500125471283852 1105 64-65 -0.9200215752676684 1105 66-67 -0.9236837572687691 1105 68-69 -0.969712150103426 1105 70-71 -0.9988527344291356 1105 72-73 -1.048716544521838 1105 74-75 -0.9944965648420876 1105 76-77 -0.9916215662314727 1105 78-79 -0.7891137258543068 1105 80-81 -0.9172126181807236 1105 82-83 -0.8482222260347072 1105 84-85 -0.6903321307837693 1105 86-87 -0.6011196238999901 1105 88-89 -0.6613107122164301 1105 90-91 -0.6448989543032901 1105 92-93 -0.5907248717025126 1105 94-95 -0.5706287022149077 1105 96-97 -0.6081281626943102 1105 98-99 -0.5827448790625773 1105 100-101 -0.39644106791282496 1106 1 0.08973980197160358 1106 2 0.05069208645787171 1106 3 0.12609980192314652 1106 4 0.09565056755720747 1106 5 0.11893148647607887 1106 6 0.09889489990717237 1106 7 0.05214931534386125 1106 8 0.02369382052958713 1106 9 0.06584258307255908 1106 10-11 0.11953460813767691 1106 12-13 0.04932941670809754 1106 14-15 0.2181926358018913 1106 16-17 0.15032993261533534 1106 18-19 0.051490256655803535 1106 20-21 0.14083545064788439 1106 22-23 0.16333826799105822 1106 24-25 0.07191374435297604 1106 26-27 0.11315614864628998 1106 28-29 0.26270199741610867 1106 30-31 0.2736496021194057 1106 32-33 0.24379920307026737 1106 34-35 -0.0357897556068707 1106 36-37 7.577799789117989E-4 1106 38-39 0.23477870767667497 1106 40-41 0.2254225605221123 1106 42-43 0.22123776399192963 1106 44-45 0.08545085496419347 1106 46-47 0.04116502605686634 1106 48-49 0.10459509600656247 1106 50-51 0.19471384140420156 1106 52-53 0.48852392625355634 1106 54-55 0.48677205332431583 1106 56-57 0.3969934176540164 1106 58-59 0.3289424854998444 1106 60-61 0.49466961593419256 1106 62-63 0.5260491571248025 1106 64-65 0.46968844875086546 1106 66-67 0.5954340929661015 1106 68-69 0.5421602859711427 1106 70-71 0.4852615555362263 1106 72-73 0.7533218426229382 1106 74-75 0.5585541078894352 1106 76-77 0.513066564786449 1106 78-79 0.3002902212624079 1106 80-81 0.31614312182395565 1106 82-83 0.38255497548718154 1106 84-85 0.5857481718922735 1106 86-87 0.516306832622206 1106 88-89 0.5844780734659736 1106 90-91 0.4487228494248967 1106 92-93 0.5852972444791753 1106 94-95 0.7234083777264289 1106 96-97 0.5506943977239942 1106 98-99 0.4469833714103508 1106 100-101 0.521534573467072 1107 1 0.17495529569773538 1107 2 0.1325978875126097 1107 3 0.19913126218865074 1107 4 0.12045507746810102 1107 5 0.09758215023313 1107 6 0.044084934458908265 1107 7 0.03287308330167349 1107 8 0.047625444461210975 1107 9 -0.003224520037100831 1107 10-11 0.08454642846864857 1107 12-13 0.09439215538785817 1107 14-15 0.2500529740452109 1107 16-17 0.2355817965906013 1107 18-19 0.12339423919807757 1107 20-21 0.2381986075855167 1107 22-23 0.22023952276465053 1107 24-25 0.17966060741898815 1107 26-27 0.2714940282607685 1107 28-29 0.14158906779253755 1107 30-31 0.0839969379986627 1107 32-33 0.1625298813754199 1107 34-35 0.1879054617053626 1107 36-37 0.24383834008074246 1107 38-39 0.11975779478341764 1107 40-41 0.23055076565031385 1107 42-43 0.05011574180608136 1107 44-45 0.16812043125078446 1107 46-47 0.31346908133964746 1107 48-49 0.3611144631642276 1107 50-51 0.3456685604440253 1107 52-53 0.3466435843732114 1107 54-55 0.32017812715591276 1107 56-57 0.25349459968711585 1107 58-59 0.029015225998115568 1107 60-61 0.1506979847285166 1107 62-63 0.27422155210571475 1107 64-65 0.4804904127443166 1107 66-67 0.281995285910277 1107 68-69 0.1504163325250616 1107 70-71 0.42186821129180885 1107 72-73 0.15501305920776787 1107 74-75 0.15860866326313783 1107 76-77 0.3064289943190843 1107 78-79 0.4763222270816456 1107 80-81 0.42569031222220843 1107 82-83 0.384137081324603 1107 84-85 0.4384668480152065 1107 86-87 0.431418671138303 1107 88-89 0.22637660047088204 1107 90-91 0.15554227113793218 1107 92-93 0.21048364200599678 1107 94-95 0.40573243664622893 1107 96-97 0.3734985439323957 1107 98-99 0.47591590459820665 1107 100-101 0.5312090597373 1108 1 0.07399425570093143 1108 2 0.06174145864684988 1108 3 0.06023373324446624 1108 4 0.09192162716171026 1108 5 0.07321187734030588 1108 6 -0.0705008081206131 1108 7 0.03628922824192671 1108 8 0.02943613420347191 1108 9 0.10142914666944591 1108 10-11 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74-75 -0.5247230655003747 2316 76-77 -0.7677652442055631 2316 78-79 -0.5270420209634494 2316 80-81 -0.3705806675281238 2316 82-83 -0.2786080167146139 2316 84-85 -0.32283948575670607 2316 86-87 -0.39343611529345424 2316 88-89 -0.4861871811792575 2316 90-91 -0.5165221683858263 2316 92-93 -0.43679768187107015 2316 94-95 -0.19369954563931913 2316 96-97 -0.05616995194386121 2316 98-99 -0.04131931463951943 2316 100-101 0.09865019876792758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 1.0 5 7.0 6 21.0 7 47.0 8 124.0 9 271.0 10 496.0 11 891.0 12 1363.0 13 1968.0 14 2379.0 15 3391.0 16 4791.0 17 7616.0 18 7379.0 19 3956.0 20 2630.0 21 2536.0 22 3041.0 23 3163.0 24 3577.0 25 4189.0 26 3865.0 27 3413.0 28 4148.0 29 5222.0 30 6810.0 31 8722.0 32 11082.0 33 14622.0 34 21089.0 35 32959.0 36 55569.0 37 122200.0 38 222533.0 39 80760.0 40 73.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.17760743120599 21.30024459403883 15.891036602523863 21.63111137223131 2 15.90277412792585 22.44004538526463 40.282204833468846 21.374975653340673 3 17.706665100202198 27.829529504152163 33.16967901906346 21.29412637658218 4 12.85493397655936 20.775427528836936 38.014658131552096 28.354980363051613 5 12.472600346881928 37.70485974778407 35.519689104754015 14.302850800579991 6 26.23874256847208 38.3304529560709 19.886660503999035 15.544143971457986 7 22.990357177086008 35.538548104361375 23.940263345833863 17.530831372718755 8 23.567102484750492 34.34177453911387 22.798366377804502 19.292756598331135 9 25.16424333674444 16.994741121584898 22.93084312094802 34.91017242072264 10-11 22.600965209783183 27.67913730897534 29.636763301005093 20.083134180236385 12-13 24.219225219139425 26.76387818622658 29.06869705080572 19.94819954382827 14-15 21.887509072036025 28.002467130052228 28.149088236226156 21.960935561685595 16-17 19.62079029460595 30.65189566206728 29.311576168113056 20.415737875213715 18-19 19.270883250425875 28.307049246722087 32.55341579765839 19.868651705193646 20-21 20.889433704433102 26.941626758756293 32.550787904270486 19.61815163254012 22-23 20.782540276330717 27.501677214309346 32.02080364071442 19.69497886864552 24-25 20.181371018355062 27.984436687865006 32.52937830225721 19.304813991522725 26-27 19.669348667887864 28.330434917028 32.79925182213771 19.200964592946416 28-29 19.452620643597417 28.04561700759356 33.06483279329194 19.43692955551708 30-31 20.173039184239432 27.71633569354411 32.687927371153904 19.42269775106256 32-33 19.859639359720514 27.993523005696353 32.417820236356185 19.72901739822694 34-35 19.893745221974783 27.815546776142224 32.575831946466025 19.714876055416976 36-37 19.582734624817487 28.075365002856024 32.545833568436734 19.796066803889754 38-39 19.486293217252584 27.720024238451956 32.893264245500895 19.90041829879457 40-41 19.608954005682435 27.63152606406495 32.8782697949934 19.88125013525922 42-43 20.080274413902483 27.495725808695543 32.350913424825244 20.073086352576727 44-45 19.83193848874489 28.046346764444912 31.963685605018348 20.158029141791854 46-47 20.2098140997301 27.866567940318998 31.391268592345718 20.532349367605185 48-49 20.05523213573533 28.076335665459894 31.24588425520864 20.622547943596135 50-51 20.031658386226127 28.27018144830934 30.936720327219103 20.761439838245433 52-53 20.287723409583464 28.511329312141175 30.83160459170266 20.3693426865727 54-55 20.30287244205495 28.600368523402164 30.258337378228056 20.83842165631483 56-57 20.53930555598495 29.010474473880286 29.75239061007318 20.697829360061583 58-59 19.93901732093584 29.058826257332992 30.601711224986666 20.4004451967445 60-61 20.39664983235249 29.54769954227361 29.869622951554586 20.186027673819318 62-63 20.121885703552138 29.683744939787626 29.853803633226683 20.340565723433546 64-65 20.219758475912517 29.39563327417869 29.944643000686362 20.439965249222436 66-67 20.02425524119588 29.661964798209222 29.851801867607197 20.4619780929877 68-69 20.166923410474993 29.611050521461745 29.655262500898537 20.566763567164724 70-71 20.12225896981862 29.304357437059974 29.935902539088012 20.637481054033394 72-73 20.32500403878158 29.227560800088426 29.63182544424665 20.815609716883344 74-75 20.290077999816035 29.51648771257481 29.26960295148645 20.923831336122703 76-77 19.946853529533158 29.567013942832766 29.435461742811587 21.050670784822493 78-79 20.117729896762196 29.39367402761922 29.419875731271 21.06872034434758 80-81 20.0625175850032 29.438841291288043 29.325756193785963 21.172884929922798 82-83 20.2243960129706 29.385597068860896 29.195212163608886 21.194794754559616 84-85 20.2199912040619 29.20460140410379 29.312038609376103 21.26336878245821 86-87 19.985453544867543 29.142995827748948 29.40594503264064 21.465605594742872 88-89 20.260951426954932 29.200249805998435 29.082457621092527 21.456341145954102 90-91 20.145662584672273 29.136304192572027 29.203779219855747 21.514254002899957 92-93 20.1595749614318 29.108247566106975 29.156167974945358 21.57600949751587 94-95 20.278216618797785 29.014184441016162 29.078413247055984 21.62918569313007 96-97 20.27019402370207 28.989628315109396 29.056330484127894 21.683847177060635 98-99 20.310833413200683 28.997025843012743 28.97190627386359 21.720234469922985 100-101 20.056345648477354 29.332895347039727 28.84217035090431 21.768588653578604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1191.0 1 717.5 2 264.5 3 252.5 4 195.5 5 192.0 6 312.5 7 631.0 8 1008.0 9 1160.5 10 971.5 11 714.5 12 672.0 13 769.5 14 878.0 15 934.5 16 988.5 17 1058.0 18 1190.0 19 1357.5 20 1436.5 21 1561.5 22 1777.5 23 2063.0 24 2427.5 25 2997.0 26 3964.5 27 5404.0 28 7231.5 29 9664.0 30 12661.5 31 15243.0 32 17851.5 33 20792.5 34 23107.5 35 24530.0 36 26210.0 37 27192.0 38 26242.5 39 25475.5 40 24732.0 41 23997.5 42 23924.0 43 24325.5 44 24862.0 45 24698.0 46 24080.0 47 23219.0 48 22558.5 49 21588.0 50 20038.5 51 18163.5 52 15981.5 53 14168.5 54 11987.0 55 9887.0 56 8407.0 57 7065.5 58 6290.5 59 5681.5 60 4872.0 61 3920.5 62 2976.0 63 2095.5 64 1356.5 65 896.5 66 649.0 67 490.0 68 339.5 69 235.5 70 177.0 71 131.5 72 97.0 73 77.0 74 74.5 75 70.5 76 45.5 77 20.0 78 10.0 79 5.5 80 2.0 81 1.5 82 4.5 83 4.5 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018549835679372274 2 0.0 3 0.0021641474959267653 4 0.025660606023131645 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.729098199738448E-5 14-15 7.729098199738448E-5 16-17 0.0012366557119581516 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.5458196399476895E-4 28-29 0.006492442487780296 30-31 7.729098199738448E-5 32-33 1.5458196399476895E-4 34-35 0.009970536677662597 36-37 0.004251004009856146 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5458196399476895E-4 60-61 0.0020095655319319963 62-63 0.01089802846163121 64-65 0.0021641474959267653 66-67 0.005487659721814297 68-69 0.003478094189882301 70-71 7.729098199738448E-5 72-73 0.008115553109725369 74-75 0.007033479361761987 76-77 0.00224143847792415 78-79 2.3187294599215342E-4 80-81 0.007419934271748909 82-83 0.009043044893693983 84-85 0.0025506024059136875 86-87 0.0020095655319319963 88-89 6.183278559790758E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 7.729098199738448E-5 98-99 0.0 100-101 9.274917839686137E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 646906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.22403045144396 #Duplication Level Percentage of deduplicated Percentage of total 1 64.98193022210941 33.936183027130475 2 19.32956600347893 20.18935687157759 3 7.439447209851317 11.65553752887555 4 3.3343272176242422 6.965280245931472 5 1.704532538892807 4.450877960830751 6 0.9571283849854698 2.999106115405353 7 0.5521203961760626 2.0183766667933396 8 0.37591272351228566 1.5705342015832673 9 0.24103885177078357 1.1329218301372639 >10 1.0196103299404002 8.932635083560015 >50 0.044209852756842916 1.562556936929317 >100 0.017209170533607217 1.5090954040845899 >500 0.001186839347138834 0.5181656259617448 >1k 0.001780259020708251 2.559372501199204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4215 0.6515629782379512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 0.6498625766340087 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 3430 0.5302161365020575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2060 0.31843884582922405 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1416 0.21888806101659283 RNA PCR Primer, Index 33 (95% over 22bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1192 0.18426170108176457 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 974 0.15056283293090497 RNA PCR Primer, Index 33 (95% over 24bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 933 0.14422497240711943 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 784 0.12119225977189885 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 653 0.10094202248858414 RNA PCR Primer, Index 33 (96% over 29bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.3187294599215342E-4 0.0 22-23 0.0 0.0 0.0 6.183278559790757E-4 0.0 24-25 0.0 0.0 0.0 0.0013139466939555361 0.0 26-27 0.0 0.0 0.0 0.0021641474959267653 0.0 28-29 0.0 0.0 0.0 0.006260569541788143 0.0 30-31 0.0 0.0 0.0 0.012598430065573669 0.0 32-33 0.0 0.0 0.0 0.025119569149149954 0.0 34-35 0.0 0.0 0.0 0.0473020809823993 0.0 36-37 0.0 0.0 0.0 0.07234435914955187 0.0 38-39 0.0 0.0 0.0 0.09444958000080383 0.0 40-41 0.0 0.0 0.0 0.11377232550014994 0.0 42-43 0.0 0.0 0.0 0.13943293152328157 0.0 44-45 0.0 0.0 0.0 0.1736728365481229 0.0 46-47 0.0 0.0 0.0 0.20822190550095376 0.0 48-49 0.0 0.0 0.0 0.24910883497757014 0.0 50-51 0.0 0.0 0.0 0.2862857973183121 0.0 52-53 0.0 0.0 0.0 0.33126914884078984 0.0 54-55 0.0 0.0 0.0 0.3873051107888936 0.0 56-57 0.0 0.0 0.0 0.44983351522477766 0.0 58-59 0.0 0.0 0.0 0.5020822190550096 0.0 60-61 0.0 0.0 0.0 0.558272762967108 0.0 62-63 0.0 0.0 0.0 0.6319310688106154 0.0 64-65 0.0 0.0 0.0 0.7311726896952571 0.0 66-67 0.0 0.0 0.0 0.853369732233122 0.0 68-69 0.0 0.0 0.0 0.984841692610673 0.0 70-71 0.0 0.0 0.0 1.1037925139046476 0.0 72-73 0.0 0.0 0.0 1.2386652774900835 0.0 74-75 0.0 0.0 0.0 1.3994305200446433 0.0 76-77 0.0 0.0 0.0 1.5775089425666171 0.0 78-79 0.0 0.0 0.0 1.7777698769218402 0.0 80-81 0.0 0.0 0.0 1.9660507090674688 0.0 82-83 0.0 0.0 0.0 2.1597419099529143 0.0 84-85 0.0 0.0 0.0 2.382571811051374 0.0 86-87 0.0 0.0 0.0 2.65100339152829 0.0 88-89 0.0 0.0 0.0 2.9837410690270305 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2290 0.0 63.068466 1 GTGGTAT 795 0.0 40.63647 1 GTATCAA 4090 0.0 40.539303 1 TGGTATC 870 0.0 38.219574 2 CGGAGAT 75 6.908789E-6 38.007053 1 GCGGTAT 55 0.0025764497 34.551865 1 ACCGTGC 55 0.00258036 34.541183 8 TATCAAC 5080 0.0 32.633884 2 TCAACGC 5105 0.0 32.38102 4 ATCAACG 5130 0.0 32.22322 3 CAACGCA 5200 0.0 31.784529 5 AACGCAG 5400 0.0 30.78323 6 CGTCAGA 100 4.9487622E-5 28.500881 2 CATTCCG 70 0.008393967 27.139502 9 TGCTCCG 70 0.008393967 27.139502 5 ACGCAGA 6195 0.0 26.98617 7 CGCAGAG 6165 0.0 26.886372 8 GCAGAGT 6535 0.0 24.928057 9 GAGTACT 3615 0.0 24.830954 12-13 GGTCGGG 115 1.279294E-4 24.787207 1 >>END_MODULE