FastQCFastQC Report
Fri 10 Feb 2017
SRR2031676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606037
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.031.4866051
GTATCAA29100.031.0881041
ATCAACG29750.028.4367493
TCAACGC29600.028.259724
CAACGCA30050.027.994695
AACGCAG31400.026.7777276
GTACATG34750.026.0334931
TATCAAC34000.024.9078042
ACGCAGA36350.023.1312437
CGCAGAG36200.022.8355488
TACATGG36450.022.7115122
GGACCGT1100.002876453821.5927246
ACATGGG35300.021.1383863
TACACCG2308.97071E-820.6642235
TGCACCG2401.4053876E-719.8032135
GTGTAGC11400.019.2126221
CGTATAC1250.006011874419.0110843
ACACCGT3557.8216544E-1118.7339696
CCTATAC5100.018.6383173
GTATAGA7700.018.5508651
Produced by FastQC (version 0.11.5)