FastQCFastQC Report
Fri 10 Feb 2017
SRR2031676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606037
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG34900.027.5056211
GGTATCA21150.026.2888131
GTATCAA29200.025.2257631
TACATGG36250.025.1569962
ACATGGG34450.025.092723
TCAACGC29450.024.3532664
CAACGCA30450.023.864715
TATACCG1000.001654852323.7477265
ATCAACG30850.023.5560153
TACACCG1652.4329038E-623.0281015
AACGCAG31300.022.9131436
CATGGGG21850.021.9551054
CGTTAGG1353.784951E-421.1209261
CCTATAC6100.021.0232333
TATCAAC34550.020.7584272
CTAACGC1150.00372530620.650843
ACGCAGA35750.019.6624537
CGTACTC1250.006034415718.9987742
CGCAGAG37050.018.844358
GTATAGG5300.017.9328611
Produced by FastQC (version 0.11.5)