FastQCFastQC Report
Fri 10 Feb 2017
SRR2031675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565140
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37550.027.1385041
GGTATCA26750.026.5243421
GTACATG40950.024.4201031
TCAACGC40350.024.2644564
CAACGCA40600.024.2321115
ATCAACG41000.023.7623353
TACATGG42600.023.2275892
AACGCAG43400.022.989926
TATCAAC44000.022.1641752
ACGCAGA47800.020.37677
CGCAGAG47750.020.1990328
ACATGGG45650.020.1966843
GGATACG1250.00594539119.047681
GTGTACG2001.25773495E-519.047681
CCTATAC5050.018.8216513
AGAGTAC38150.018.43122710-11
GTATAGA7250.018.3908631
GAGTACA33700.018.3694271
TAGCACG1300.00753890918.279894
ACCGGTC650.007641358318.23784320-21
Produced by FastQC (version 0.11.5)