FastQCFastQC Report
Fri 10 Feb 2017
SRR2031675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565140
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC1601.8616665E-623.7479578
GGTATCA32500.023.3900741
GTACATG43000.022.2086721
TACATGG42950.020.7918192
GTATCAA40450.020.554861
CGTACAC1951.0332351E-519.4867483
TATAGTA8800.019.4320092
CAACGCA42600.018.9537665
TCAACGC42400.018.9323644
GAGTACA35400.018.9239081
GCTACAC7800.018.8777873
ACATGGG44050.018.8701843
GTATAGT5050.018.8162731
TAGACAG11000.018.5665825
AACGCAG44050.018.545516
TAATACT13500.018.295894
TCCGTAG1300.00755414218.2734951
ATCAACG44200.018.2688263
CCTAGAC6000.018.2079283
TTAATAC10800.018.0320073
Produced by FastQC (version 0.11.5)