##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031675_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1565140 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13497386815237 33.0 31.0 34.0 30.0 34.0 2 32.39610258507226 34.0 31.0 34.0 31.0 34.0 3 32.502492428792316 34.0 31.0 34.0 31.0 34.0 4 36.0527160509603 37.0 35.0 37.0 35.0 37.0 5 35.66006108079788 37.0 35.0 37.0 33.0 37.0 6 35.73859463051229 37.0 35.0 37.0 33.0 37.0 7 35.71130633681332 37.0 35.0 37.0 33.0 37.0 8 35.82598809052225 37.0 35.0 37.0 35.0 37.0 9 37.7014854901159 39.0 38.0 39.0 35.0 39.0 10-11 37.64143846556857 39.0 37.5 39.0 35.0 39.0 12-13 37.653242201975544 39.0 37.5 39.0 35.0 39.0 14-15 39.037480033735 40.0 38.5 41.0 36.0 41.0 16-17 38.98358102150607 40.0 38.0 41.0 36.0 41.0 18-19 38.973407171243466 40.0 38.5 41.0 35.5 41.0 20-21 38.97987879678495 40.0 39.0 41.0 35.0 41.0 22-23 38.906112232771505 40.0 38.0 41.0 35.0 41.0 24-25 38.826067955582246 40.0 38.0 41.0 35.0 41.0 26-27 38.68591116449646 40.0 38.0 41.0 34.0 41.0 28-29 38.5779358396054 40.0 38.0 41.0 34.0 41.0 30-31 38.46550883627024 40.0 38.0 41.0 34.0 41.0 32-33 38.3205403350499 40.0 38.0 41.0 34.0 41.0 34-35 38.198558275936975 40.0 38.0 41.0 34.0 41.0 36-37 38.05435200684923 40.0 38.0 41.0 33.0 41.0 38-39 37.92798950892572 40.0 38.0 41.0 33.0 41.0 40-41 37.8103616928837 40.0 37.0 41.0 33.0 41.0 42-43 37.65908417138403 40.0 37.0 41.0 32.5 41.0 44-45 37.60357603792632 40.0 37.0 41.0 32.5 41.0 46-47 37.650065489349196 40.0 37.0 41.0 32.5 41.0 48-49 37.797212070485706 40.0 37.0 41.0 33.0 41.0 50-51 37.729576267937695 40.0 37.0 41.0 33.0 41.0 52-53 37.6217293660631 40.0 37.0 41.0 32.0 41.0 54-55 37.50250616558263 40.0 37.0 41.0 32.0 41.0 56-57 37.384766538456624 40.0 36.0 41.0 32.0 41.0 58-59 37.18809946714032 39.0 36.0 41.0 31.0 41.0 60-61 36.89381524975401 39.0 35.0 41.0 31.0 41.0 62-63 36.70244642651775 39.0 35.0 41.0 31.0 41.0 64-65 36.41661864114393 38.5 35.0 40.0 30.0 41.0 66-67 36.136150759676454 38.0 35.0 40.0 30.0 41.0 68-69 35.853799979554545 37.0 35.0 40.0 30.0 41.0 70-71 35.53259005584165 37.0 34.0 39.5 29.0 41.0 72-73 35.172343688104576 36.0 34.0 39.0 29.0 41.0 74-75 34.81589538316061 36.0 34.0 39.0 29.0 40.5 76-77 34.22014069028968 35.0 33.5 38.0 28.5 39.0 78-79 34.23940669844231 35.0 34.0 37.0 29.0 39.0 80-81 33.979950036418465 35.0 34.0 37.0 29.0 39.0 82-83 33.69245402967147 35.0 34.0 36.5 29.0 39.0 84-85 33.39292203892303 35.0 33.0 36.0 28.0 37.0 86-87 33.11842870286364 35.0 33.0 36.0 27.5 37.0 88-89 32.86083800810151 35.0 33.0 35.0 27.0 37.0 90-91 32.69358651622219 35.0 33.0 35.0 27.0 36.0 92-93 32.537744227353464 35.0 33.0 35.0 26.0 36.0 94-95 32.39419572689983 35.0 33.0 35.0 26.0 36.0 96-97 32.26406647328673 35.0 32.5 35.0 26.0 35.5 98-99 32.14479152024739 35.0 32.0 35.0 25.0 35.0 100-101 31.62489361974009 34.5 31.5 35.0 24.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2080686074891176 1101 2 0.17473757289942426 1101 3 0.22502108468672333 1101 4 0.22579803893358275 1101 5 0.27838050801616987 1101 6 0.2197931711953487 1101 7 0.3015227942777159 1101 8 0.15707073055812515 1101 9 0.2559239663743682 1101 10-11 0.04949191498577221 1101 12-13 0.09033504276646909 1101 14-15 0.17126053962012122 1101 16-17 0.16668685609076306 1101 18-19 0.11544904364578912 1101 20-21 0.04386129633854807 1101 22-23 0.09570408912841799 1101 24-25 0.11538781325715064 1101 26-27 0.19619709451023226 1101 28-29 0.10927520053646589 1101 30-31 -0.04724737306264615 1101 32-33 -0.016088597201751043 1101 34-35 -7.76698973268708E-4 1101 36-37 0.03606150981767797 1101 38-39 0.038404626695999866 1101 40-41 -0.09955145887288097 1101 42-43 -0.23935901503511303 1101 44-45 -0.142946704505448 1101 46-47 0.03411239805705435 1101 48-49 -0.020186359836252166 1101 50-51 0.12126680969614512 1101 52-53 -0.0750406310262548 1101 54-55 0.2236385258142093 1101 56-57 0.2012177130969519 1101 58-59 0.03519939195142996 1101 60-61 -0.22777695449345714 1101 62-63 -0.1737506342306787 1101 64-65 -0.17787429121156606 1101 66-67 -0.1746488356116842 1101 68-69 -0.10814407257647218 1101 70-71 -0.06983442407350537 1101 72-73 -0.21262729196168806 1101 74-75 -0.16423315380093584 1101 76-77 -0.06971254341151933 1101 78-79 -0.00856826968684743 1101 80-81 -0.07405504338655078 1101 82-83 -0.021426282645442996 1101 84-85 -0.1293874013198959 1101 86-87 -0.22799203436121473 1101 88-89 -0.19040514261180164 1101 90-91 -0.0759995009940937 1101 92-93 -0.4078805969679067 1101 94-95 -0.026784681241501573 1101 96-97 -0.2761747789356477 1101 98-99 -0.5537141728513362 1101 100-101 -0.43877244409584293 1102 1 0.13908662355163415 1102 2 0.2242788330179124 1102 3 0.16669944264677383 1102 4 0.2197118604247379 1102 5 0.004380179777591309 1102 6 0.09704729667777912 1102 7 0.11385821174953747 1102 8 0.05699131799929802 1102 9 0.0629387424044836 1102 10-11 0.17934071347375635 1102 12-13 0.12320840091182106 1102 14-15 0.28423840469289274 1102 16-17 0.2585544806493587 1102 18-19 0.32222875850175825 1102 20-21 0.19435180124360585 1102 22-23 0.16625552993693304 1102 24-25 0.23500906829322332 1102 26-27 0.229185865574415 1102 28-29 -0.08435379457701941 1102 30-31 -0.13083253244365523 1102 32-33 0.1520222309653505 1102 34-35 0.2690552640522412 1102 36-37 0.3797331230043923 1102 38-39 0.2776029304437415 1102 40-41 0.24000631905784076 1102 42-43 0.2526573757170283 1102 44-45 0.34574071178047916 1102 46-47 0.4676702924595304 1102 48-49 0.4360504378258483 1102 50-51 0.3629531089120803 1102 52-53 0.2663216649496505 1102 54-55 0.18311403233398948 1102 56-57 0.2084784033509166 1102 58-59 0.12021556564258162 1102 60-61 0.4306606298823894 1102 62-63 0.23267225352761045 1102 64-65 0.3450772618333815 1102 66-67 0.26334080899064105 1102 68-69 0.17560652963542367 1102 70-71 0.18219377267904946 1102 72-73 0.21930608619562264 1102 74-75 0.362641114941745 1102 76-77 0.4193144939176747 1102 78-79 0.08115203928095127 1102 80-81 -0.018456722697457906 1102 82-83 -0.31388050130527745 1102 84-85 -0.19846706270474357 1102 86-87 -0.035468197358135 1102 88-89 0.09011522141536688 1102 90-91 0.1266565843902896 1102 92-93 0.25670881363208053 1102 94-95 0.17293869834523434 1102 96-97 0.20970249160175314 1102 98-99 0.17657589475522428 1102 100-101 0.10236114371062044 1103 1 0.13763470286798452 1103 2 0.12462749634026693 1103 3 0.17330644060093192 1103 4 0.08295509710500681 1103 5 -0.04439727265303617 1103 6 0.008538818877497079 1103 7 0.10689911038220146 1103 8 0.12377764586423012 1103 9 0.17241255016006818 1103 10-11 0.2009616842673907 1103 12-13 0.12916734646327654 1103 14-15 0.15429808015067437 1103 16-17 0.2092078955465766 1103 18-19 0.1752716200097737 1103 20-21 0.09211230212082455 1103 22-23 -0.11682043738624515 1103 24-25 -0.11449758221176154 1103 26-27 -0.044677740895387785 1103 28-29 0.011179551032718393 1103 30-31 0.06022404086164812 1103 32-33 0.05390578186757011 1103 34-35 -0.01601696773703054 1103 36-37 -0.015031969382278021 1103 38-39 0.07183693351554865 1103 40-41 0.07633084508415067 1103 42-43 0.03674968751876406 1103 44-45 -0.016097652517046868 1103 46-47 0.1374797518998463 1103 48-49 -0.0386643887632232 1103 50-51 0.054983025560702004 1103 52-53 0.017779150486084916 1103 54-55 0.011757543816798943 1103 56-57 -0.01202686504699102 1103 58-59 0.11796837690503281 1103 60-61 0.14808296983215996 1103 62-63 0.10308150292003404 1103 64-65 0.2216528740177779 1103 66-67 0.011594457004598269 1103 68-69 0.14736287812067417 1103 70-71 0.12989832149089864 1103 72-73 0.06677043572504715 1103 74-75 0.12217876673575745 1103 76-77 0.053146168250350456 1103 78-79 0.21566262565454508 1103 80-81 0.3208991304147162 1103 82-83 0.28668347123065985 1103 84-85 1.9717026630416967E-4 1103 86-87 -0.07555942047230957 1103 88-89 0.0020898740167893948 1103 90-91 0.0809959554202706 1103 92-93 -0.08813499612684694 1103 94-95 0.005178116524696463 1103 96-97 -0.06975885088356648 1103 98-99 -0.06586501156705538 1103 100-101 -0.11520487062039209 1104 1 0.18450065693357942 1104 2 0.15541621439658826 1104 3 0.16725574257048947 1104 4 0.07527248822352561 1104 5 0.17444294384714 1104 6 0.07393861760849063 1104 7 0.12417890926861475 1104 8 0.2150714014739279 1104 9 0.2817049225310413 1104 10-11 0.15030590866290794 1104 12-13 0.15141913088008607 1104 14-15 0.1444653315854012 1104 16-17 0.23125131991722014 1104 18-19 0.29739683419807506 1104 20-21 0.1840044250425663 1104 22-23 0.01763007491719293 1104 24-25 0.0586481590837451 1104 26-27 0.1365653866228982 1104 28-29 0.1378983999916059 1104 30-31 0.07133851940785263 1104 32-33 0.04402495021471253 1104 34-35 0.15193288597382093 1104 36-37 0.0982231293677529 1104 38-39 0.15056497557221604 1104 40-41 -0.1085165712512719 1104 42-43 -0.040906441666408 1104 44-45 0.06991327740016118 1104 46-47 0.2173491601767381 1104 48-49 0.19565081093215042 1104 50-51 0.3171917586150528 1104 52-53 0.2506785703806358 1104 54-55 0.27522712227869306 1104 56-57 0.1525717431661775 1104 58-59 0.11037804869667411 1104 60-61 0.24208977723171188 1104 62-63 0.16090165857323768 1104 64-65 0.002133180350753605 1104 66-67 -0.1397706169947881 1104 68-69 0.022201144098822567 1104 70-71 0.022844969662578762 1104 72-73 0.18426413149800425 1104 74-75 -0.006177335639449666 1104 76-77 0.07584721383894788 1104 78-79 0.27366491421524586 1104 80-81 0.21432515239888517 1104 82-83 0.19388991183880933 1104 84-85 0.2799805146733192 1104 86-87 0.11474809758555438 1104 88-89 0.16115067046698783 1104 90-91 0.0244322739848144 1104 92-93 0.10980413359840924 1104 94-95 -0.01491430014099393 1104 96-97 0.12501990863106016 1104 98-99 0.08290309001551321 1104 100-101 -0.05226750879855757 1105 1 0.10545612128306203 1105 2 0.06692458137302992 1105 3 -0.04115540793070949 1105 4 0.06259155165896857 1105 5 -0.05826021021780292 1105 6 -0.10983198297229535 1105 7 0.0742519735316165 1105 8 -0.004266770502944439 1105 9 0.06315424165411798 1105 10-11 0.061482038269957684 1105 12-13 0.06084320928250975 1105 14-15 0.029574107870828925 1105 16-17 -0.007113382588208594 1105 18-19 0.0708090843909801 1105 20-21 -0.01600992686623215 1105 22-23 -0.10397858767129264 1105 24-25 0.04938558625246259 1105 26-27 0.12621896509428865 1105 28-29 0.11735932738584864 1105 30-31 -0.11140508094554491 1105 32-33 0.10841469586906527 1105 34-35 0.06011515148785662 1105 36-37 0.19775128706726974 1105 38-39 0.14619789475047185 1105 40-41 0.03748847241953257 1105 42-43 0.006175740718390443 1105 44-45 -0.22263127875308442 1105 46-47 0.0065839238548619505 1105 48-49 0.17674077316986114 1105 50-51 0.24762460845681744 1105 52-53 0.248042572065593 1105 54-55 0.16843727150126142 1105 56-57 -0.07035626989592458 1105 58-59 -0.022986597892227678 1105 60-61 0.08716744528883424 1105 62-63 0.09098829191366065 1105 64-65 0.1714945576866711 1105 66-67 0.12385018320799901 1105 68-69 0.11713342918365299 1105 70-71 0.19265228130775824 1105 72-73 0.4110960501431862 1105 74-75 0.094430942988879 1105 76-77 -0.004767644300819995 1105 78-79 0.053125002861577286 1105 80-81 0.1379865107231737 1105 82-83 -0.014760747789821949 1105 84-85 -0.1419916985401315 1105 86-87 -0.0730004979776524 1105 88-89 0.05755784716694734 1105 90-91 -0.005186244823647712 1105 92-93 -0.035212585443403555 1105 94-95 -0.26369578580586506 1105 96-97 -0.16865788915240643 1105 98-99 -0.2361333190996291 1105 100-101 0.014384060405600252 1106 1 0.07821294432122983 1106 2 0.04834193762940231 1106 3 0.0119333251227971 1106 4 0.1203411371795795 1106 5 0.18198546631776225 1106 6 -0.16416506114836693 1106 7 -0.05311745185665018 1106 8 0.015461360040831096 1106 9 -0.027294774660227006 1106 10-11 0.1286966279444357 1106 12-13 0.06369876366909466 1106 14-15 0.005947875769628297 1106 16-17 -0.06378686352578455 1106 18-19 -0.11222029739078465 1106 20-21 -0.030726666029011085 1106 22-23 -0.0695400666643522 1106 24-25 -0.08709331611179039 1106 26-27 0.022271706462781538 1106 28-29 0.035834315323327814 1106 30-31 -0.0913445506295929 1106 32-33 -0.05912204496249984 1106 34-35 -0.2979347275240656 1106 36-37 -0.32170611678620986 1106 38-39 -0.16917058377460137 1106 40-41 -0.19735400727369523 1106 42-43 -0.041254487072947654 1106 44-45 -0.11894613071955007 1106 46-47 0.01013012606274799 1106 48-49 0.1075408544332106 1106 50-51 0.006307815602184519 1106 52-53 0.06518052386626039 1106 54-55 0.14397799931619204 1106 56-57 -0.08171636088185608 1106 58-59 0.06496873991300589 1106 60-61 -0.08741136384023207 1106 62-63 -0.05317026957985149 1106 64-65 0.2166270096995646 1106 66-67 0.05035264831736441 1106 68-69 0.041339025471657465 1106 70-71 0.06447747802674542 1106 72-73 0.17202715042724748 1106 74-75 0.11913101228039835 1106 76-77 0.11217041415800821 1106 78-79 0.09070658365150308 1106 80-81 -0.009513280676699765 1106 82-83 0.10509771248727162 1106 84-85 0.13623960816736513 1106 86-87 0.00934757764080274 1106 88-89 0.1764268172627581 1106 90-91 0.10542714839304779 1106 92-93 0.030086964343098543 1106 94-95 0.06788727151019458 1106 96-97 -0.10000667945305253 1106 98-99 0.10129779750965895 1106 100-101 0.04972364327789691 1107 1 0.05330230982614381 1107 2 0.10542589931394275 1107 3 0.12672436343825666 1107 4 0.1403174692841347 1107 5 0.10877382366807353 1107 6 0.0922807749776382 1107 7 0.13758713929616562 1107 8 0.09474706435271685 1107 9 0.0935162172968802 1107 10-11 0.10619380036400372 1107 12-13 0.0312723505770478 1107 14-15 0.08381891715702494 1107 16-17 0.15223853582630653 1107 18-19 0.08124293010027372 1107 20-21 0.03586285307287085 1107 22-23 0.09124889809213244 1107 24-25 0.13935311345702672 1107 26-27 0.13532517915044906 1107 28-29 0.18474918769821613 1107 30-31 0.1802131320111542 1107 32-33 0.32047015510452326 1107 34-35 0.2509073608360808 1107 36-37 0.19892474782505332 1107 38-39 0.19122548693585628 1107 40-41 0.18605019011236124 1107 42-43 0.08497865216968137 1107 44-45 -0.01757649963153085 1107 46-47 0.27173804189161643 1107 48-49 0.25778224602168365 1107 50-51 0.36985349673489054 1107 52-53 0.060117060394411226 1107 54-55 0.08581973161064127 1107 56-57 0.2792504621744456 1107 58-59 0.18243114356582169 1107 60-61 0.17220502774824098 1107 62-63 0.08838853012971981 1107 64-65 0.020200931728162175 1107 66-67 0.3310589499817951 1107 68-69 0.2855232859721042 1107 70-71 0.25907086497937826 1107 72-73 0.2971606750586133 1107 74-75 0.2630714376043528 1107 76-77 0.11234321073977327 1107 78-79 0.22054314252102358 1107 80-81 0.3570135778465442 1107 82-83 0.3754955635261439 1107 84-85 0.37293688950114046 1107 86-87 0.028751411979492048 1107 88-89 -0.11722613738425025 1107 90-91 0.08317129774513887 1107 92-93 0.3541922620419342 1107 94-95 0.4163577599485322 1107 96-97 0.2479995272605322 1107 98-99 0.12123922121386244 1107 100-101 0.21170298448656766 1108 1 0.03505162112090687 1108 2 0.09767092937857313 1108 3 0.1552298896355495 1108 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-0.9281180086031284 2316 80-81 -1.3260690457767055 2316 82-83 -1.571479386125688 2316 84-85 -1.4542819501015352 2316 86-87 -1.3584512635848576 2316 88-89 -1.1172126258695911 2316 90-91 -0.8392964310688029 2316 92-93 -0.6468726586525833 2316 94-95 -0.7046789216803582 2316 96-97 -0.7132492709257932 2316 98-99 -0.7031548229104843 2316 100-101 -0.973502724768398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 1.0 11 2.0 12 3.0 13 24.0 14 82.0 15 218.0 16 525.0 17 1039.0 18 1585.0 19 2256.0 20 3317.0 21 4473.0 22 5711.0 23 6689.0 24 7519.0 25 9078.0 26 10993.0 27 13807.0 28 17847.0 29 22528.0 30 28152.0 31 34761.0 32 43967.0 33 57255.0 34 79360.0 35 117799.0 36 197736.0 37 343941.0 38 426778.0 39 127657.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.314839258992684 20.937784252348006 14.429967572460178 22.317408916199135 2 15.117795402168127 20.82665390520526 41.348412814534775 22.70713787809184 3 18.434744051159736 25.749566970814698 30.9802272900072 24.835461688018366 4 12.137382045331398 17.06994469037858 39.62823193236073 31.164441331929293 5 12.20178386598004 39.005775841138814 36.425112130544235 12.367328162336916 6 30.51950624225309 39.19949653066179 17.598745160177366 12.682252066907754 7 26.892865813920803 32.82677587947404 23.037491853763882 17.24286645284128 8 24.568664783980985 38.20987259925629 20.231800350128424 16.989662266634294 9 25.032201592189836 16.72304075034821 21.040993137994047 37.20376451946791 10-11 24.03526202128883 27.150159091199512 29.57553317914052 19.239045708371137 12-13 24.181319243007017 24.32517218906935 29.93776914525218 21.555739422671454 14-15 21.57963504862185 26.355437852204915 26.644677153481478 25.420249945691758 16-17 21.475203496172863 30.004025199023733 27.22127094061873 21.299500364184674 18-19 21.50015334091519 29.0386482998326 28.706633272422916 20.754565086829295 20-21 22.179805001469514 28.067808630537844 28.704397050743065 21.047989317249574 22-23 22.023425213012388 27.77919230519801 28.563355192307753 21.634027289481846 24-25 21.75699937385793 28.14492633246866 28.426306911841753 21.671767381831657 26-27 21.89315332813678 28.0491521525231 28.46710198448701 21.590592534853112 28-29 21.770853969491505 27.984292022673678 28.659965107584945 21.584888900249872 30-31 21.976245333882048 28.079813582137575 28.279478538653173 21.664462545327204 32-33 21.7827478692002 28.153743435092064 28.455761145967774 21.60774754973996 34-35 22.01323843234471 28.027684424396536 28.439181159512888 21.51989598374586 36-37 22.20191165007603 27.80422198653156 28.417457863194347 21.576408500198067 38-39 21.88555017442529 28.126142070357922 28.440363162400807 21.54794459281598 40-41 21.87248425061017 28.025927393076657 28.522336659979295 21.579251696333873 42-43 21.97116551873954 27.768250763509972 28.403593288779277 21.85699042897121 44-45 21.983241115810724 28.013628173837485 28.15284894642013 21.85028176393166 46-47 21.942382151117474 28.007430645181902 28.260634831388888 21.789552372311743 48-49 21.979754299503046 27.916722723636955 28.19017421707453 21.91334875978547 50-51 21.723201594400937 28.200455406199392 28.04716236953079 22.029180629868883 52-53 21.95161455205285 28.04078229423566 28.165978762283245 21.841624391428244 54-55 21.907711003643794 28.07414350400958 27.99798355021669 22.02016194212994 56-57 22.058825408589648 27.95877685063317 27.985771240911355 21.996626499865826 58-59 21.97528655583526 27.947244335969945 27.984205885735463 22.093263222459335 60-61 21.952114780896387 28.111409948704217 28.10738474582281 21.829090524576582 62-63 21.838174220836475 28.15310451461211 28.118538916646436 21.89018234790498 64-65 21.874944094458005 28.05448713853074 28.094355776480057 21.9762129905312 66-67 21.910947263503587 28.01829229334117 27.852109076504338 22.218651366650906 68-69 21.862549037146835 28.005258315550048 27.911912033428322 22.220280613874795 70-71 21.841816067572232 27.786619727308736 28.15911036712371 22.212453837995323 72-73 21.80118072504696 28.040973970379646 27.899357245997162 22.25848805857623 74-75 21.70428843426147 27.974654024560103 27.90993137994045 22.411126161237974 76-77 21.71102904532502 27.969926013008422 28.038322450387827 22.280722491278734 78-79 21.92590808344573 27.926085703679615 27.726486563157092 22.421519649717563 80-81 21.818804546052874 27.980679403078156 27.7106089476933 22.489907103175668 82-83 21.777006489851182 27.92086432524269 27.809558617278967 22.492570567627162 84-85 21.931232988742224 27.81067508337912 27.76221296497438 22.49587896290428 86-87 21.529958981305185 27.93593544347471 28.077648005929184 22.456457569290926 88-89 21.834340697956733 27.681485362331802 27.778185976973308 22.70598796273816 90-91 21.73035638984372 27.82083391901044 27.722599895217044 22.7262097959288 92-93 21.923853457198717 27.711354894769798 27.796650778844068 22.56814086918742 94-95 21.70042296535773 27.541753453365192 27.842876675568956 22.914946905708117 96-97 21.7574466181939 27.48239774077718 27.972417802880255 22.787737838148665 98-99 21.809199177070422 27.431316048404614 27.771509257957756 22.987975516567207 100-101 21.640524170361758 27.580152574210615 27.698320916978673 23.081002338448954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 15.5 2 23.0 3 47.5 4 46.5 5 35.5 6 70.5 7 138.5 8 245.5 9 319.5 10 303.0 11 279.5 12 295.0 13 327.0 14 365.5 15 413.0 16 447.5 17 495.0 18 540.0 19 594.0 20 680.0 21 767.5 22 924.0 23 1128.5 24 1345.5 25 1795.5 26 2598.0 27 3931.0 28 6568.5 29 11092.0 30 17412.0 31 25228.0 32 35233.5 33 46299.0 34 56578.5 35 65151.5 36 72747.5 37 76162.0 38 73676.0 39 68480.0 40 61302.5 41 54273.0 42 48868.0 43 45832.0 44 44881.5 45 44630.5 46 44867.0 47 45940.5 48 48063.0 49 47927.0 50 46124.5 51 45586.0 52 45157.0 53 44974.5 54 44105.5 55 42308.5 56 39699.0 57 36395.5 58 33084.5 59 28974.0 60 24037.0 61 19513.5 62 15358.5 63 11482.5 64 8395.0 65 5996.0 66 4206.5 67 3034.0 68 2184.0 69 1498.5 70 1108.0 71 827.0 72 528.0 73 339.5 74 273.5 75 203.0 76 135.5 77 88.0 78 51.0 79 22.0 80 12.5 81 15.5 82 11.0 83 6.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04657730298887001 2 7.028125279527709E-4 3 1.9167614398711937E-4 4 0.008433750335433251 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002012599511864753 24-25 0.0 26-27 0.0 28-29 0.02108437583858313 30-31 0.017155014886847182 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03268078254980385 50-51 0.010446349847298005 52-53 0.0 54-55 8.305966239441838E-4 56-57 0.0 58-59 0.0 60-61 9.583807199355969E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.9167614398711937E-4 80-81 0.01137278454323575 82-83 0.005175255887652223 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1565140.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.3373626365861 #Duplication Level Percentage of deduplicated Percentage of total 1 66.89251220606745 31.66515107970877 2 15.869125752976196 15.024051209884432 3 6.589560806979403 9.357972886074567 4 3.3151333616846093 6.277186805228363 5 2.0142658055561062 4.767501544204232 6 1.2597865146511562 3.5780982653233595 7 0.8662371463457403 2.870376734809487 8 0.608237897758819 2.303390234841919 9 0.44637091009546115 1.9017019477450634 >10 2.019435234662366 16.09584274727377 >50 0.0817594542879607 2.6338815778622937 >100 0.03607742323441945 2.962710869585344 >500 0.0012252155729891366 0.3838854543803927 >1k 2.7227012733091927E-4 0.1782486430780873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.389204799570645E-5 0.0 5 0.0 0.0 0.0 6.389204799570645E-5 0.0 6 0.0 0.0 0.0 6.389204799570645E-5 0.0 7 0.0 0.0 0.0 6.389204799570645E-5 0.0 8 0.0 0.0 0.0 6.389204799570645E-5 0.0 9 0.0 0.0 0.0 6.389204799570645E-5 0.0 10-11 1.277840959914129E-4 0.0 0.0 6.389204799570645E-5 0.0 12-13 1.9167614398711937E-4 0.0 0.0 3.194602399785323E-4 6.389204799570645E-5 14-15 2.555681919828258E-4 0.0 0.0 5.111363839656516E-4 1.277840959914129E-4 16-17 3.514062639763855E-4 0.0 0.0 0.001533409151896955 1.277840959914129E-4 18-19 3.8335228797423875E-4 0.0 0.0 0.00207649155986046 1.277840959914129E-4 20-21 3.8335228797423875E-4 0.0 0.0 0.0037376848077488277 1.277840959914129E-4 22-23 3.8335228797423875E-4 0.0 0.0 0.006996179255529856 1.277840959914129E-4 24-25 4.791903599677984E-4 0.0 0.0 0.011053324303257218 1.277840959914129E-4 26-27 5.111363839656516E-4 0.0 0.0 0.013385384055100502 1.277840959914129E-4 28-29 5.111363839656516E-4 0.0 0.0 0.01661193247888368 1.277840959914129E-4 30-31 5.430824079635048E-4 0.0 0.0 0.022426108846492966 1.277840959914129E-4 32-33 5.750284319613581E-4 0.0 0.0 0.03239326833382317 1.9167614398711937E-4 34-35 6.708665039549177E-4 0.0 0.0 0.04660924901286786 1.9167614398711937E-4 36-37 7.667045759484775E-4 0.0 0.0 0.07159103977918908 1.9167614398711937E-4 38-39 7.667045759484775E-4 0.0 0.0 0.11040545893658076 1.9167614398711937E-4 40-41 8.305966239441839E-4 0.0 0.0 0.15816476481337133 1.9167614398711937E-4 42-43 8.305966239441839E-4 0.0 0.0 0.20228222395440665 1.9167614398711937E-4 44-45 8.305966239441839E-4 0.0 0.0 0.2524374816310362 1.9167614398711937E-4 46-47 8.625426479420372E-4 0.0 0.0 0.316904558058704 2.555681919828258E-4 48-49 8.944886719398904E-4 0.0 0.0 0.3828411515902731 2.555681919828258E-4 50-51 8.944886719398904E-4 0.0 0.0 0.452291807761606 2.555681919828258E-4 52-53 9.583807199355968E-4 0.0 0.0 0.5248412282607307 3.194602399785323E-4 54-55 9.583807199355968E-4 0.0 0.0 0.6080925667991361 3.8335228797423875E-4 56-57 9.583807199355968E-4 0.0 0.0 0.7066779968565112 3.8335228797423875E-4 58-59 9.583807199355968E-4 0.0 0.0 0.8128665806253753 3.8335228797423875E-4 60-61 9.583807199355968E-4 0.0 0.0 0.9247096106418595 4.472443359699452E-4 62-63 9.583807199355968E-4 0.0 0.0 1.0401625413701012 4.472443359699452E-4 64-65 9.583807199355968E-4 0.0 0.0 1.167052148689574 4.472443359699452E-4 66-67 9.583807199355968E-4 0.0 0.0 1.307039625848167 4.472443359699452E-4 68-69 0.0010222727679313033 0.0 0.0 1.4725200301570465 4.472443359699452E-4 70-71 0.001118110839924863 0.0 0.0 1.653749824296868 4.472443359699452E-4 72-73 0.0011500568639227161 0.0 0.0 1.8415604993802472 8.305966239441839E-4 74-75 0.0011500568639227161 0.0 0.0 2.0334922115593494 8.305966239441839E-4 76-77 0.0011500568639227161 0.0 0.0 2.2422275323613223 8.305966239441839E-4 78-79 0.0011500568639227161 0.0 0.0 2.482717201017161 8.305966239441839E-4 80-81 0.0011500568639227161 0.0 0.0 2.752629157775023 8.625426479420372E-4 82-83 0.0012139489119184226 0.0 0.0 3.0486410161391313 0.0010861648159270097 84-85 0.001277840959914129 0.0 0.0 3.3287757005763066 0.0010861648159270097 86-87 0.001277840959914129 0.0 0.0 3.6212096042526545 0.0010861648159270097 88-89 0.001277840959914129 0.0 0.0 3.960220810917873 0.0010861648159270097 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGGC 160 1.8616665E-6 23.747957 8 GGTATCA 3250 0.0 23.390074 1 GTACATG 4300 0.0 22.208672 1 TACATGG 4295 0.0 20.791819 2 GTATCAA 4045 0.0 20.55486 1 CGTACAC 195 1.0332351E-5 19.486748 3 TATAGTA 880 0.0 19.432009 2 CAACGCA 4260 0.0 18.953766 5 TCAACGC 4240 0.0 18.932364 4 GAGTACA 3540 0.0 18.923908 1 GCTACAC 780 0.0 18.877787 3 ACATGGG 4405 0.0 18.870184 3 GTATAGT 505 0.0 18.816273 1 TAGACAG 1100 0.0 18.566582 5 AACGCAG 4405 0.0 18.54551 6 TAATACT 1350 0.0 18.29589 4 TCCGTAG 130 0.007554142 18.273495 1 ATCAACG 4420 0.0 18.268826 3 CCTAGAC 600 0.0 18.207928 3 TTAATAC 1080 0.0 18.032007 3 >>END_MODULE